5N4I

Crystal structure of OphA-DeltaC6 mutant W400A in complex with SAM

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0008168	molecular_function	methyltransferase activity
A	0032259	biological_process	methylation

Functional Information from PDB Data

site_id	AC1
Number of Residues	21
Details	binding site for residue SAM A 501

Chain	Residue
A	ILE19
A	PHE171
A	GLN172
A	TYR211
A	ILE212
A	ALA213
A	GLY242
A	VAL243
A	SER244
A	THR245
A	HOH630
A	TYR98
A	HOH701
A	HOH703
A	GLY99
A	HIS100
A	VAL103
A	PHE104
A	VAL105
A	SER129
A	ALA130

---

site_id	AC2
Number of Residues	7
Details	binding site for residue GOL A 502

Chain	Residue
A	GLU131
A	PHE135
A	PRO142
A	CYS147
A	THR149
A	HOH658
A	HOH768

---

site_id	AC3
Number of Residues	1
Details	binding site for residue SCN A 503

Chain	Residue
A	LEU333

---

site_id	AC4
Number of Residues	3
Details	binding site for residue SCN A 504

Chain	Residue
A	VAL327
A	LYS330
A	HOH671

---

site_id	AC5
Number of Residues	2
Details	binding site for residue SCN A 505

Chain	Residue
A	ASP334
A	HOH634

---

site_id	AC6
Number of Residues	6
Details	binding site for residue BCT A 507

Chain	Residue
A	TYR280
A	PRO281
A	ALA282
A	GLN284
A	GLU291
A	VAL292

---

site_id	AC7
Number of Residues	2
Details	binding site for residue MLI A 508

Chain	Residue
A	ALA302
A	GLN305

---

site_id	AC8
Number of Residues	3
Details	binding site for residues MVA A 401 and IML A 402

Chain	Residue
A	ALA400
A	VAL403
A	VAL404

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	3
Details	ACT_SITE: ACT_SITE => ECO:0000269|PubMed:30151425

Chain	Residue	Details
A	ARG72
A	TYR76
A	TYR98

---

site_id	SWS_FT_FI2
Number of Residues	1
Details	BINDING: BINDING => ECO:0000269|PubMed:30151425, ECO:0007744|PDB:5N0P, ECO:0007744|PDB:5N0T, ECO:0007744|PDB:5N0U

Chain	Residue	Details
A	TYR98

---

site_id	SWS_FT_FI3
Number of Residues	4
Details	BINDING: BINDING => ECO:0000269|PubMed:30151425, ECO:0007744|PDB:5N0O, ECO:0007744|PDB:5N0P, ECO:0007744|PDB:5N0Q, ECO:0007744|PDB:5N0R, ECO:0007744|PDB:5N0S, ECO:0007744|PDB:5N0T, ECO:0007744|PDB:5N0U, ECO:0007744|PDB:5N0V, ECO:0007744|PDB:5N0W, ECO:0007744|PDB:5N0X, ECO:0007744|PDB:5N4I, ECO:0007744|PDB:6GEW, ECO:0007744|PDB:6QZY, ECO:0007744|PDB:6QZZ, ECO:0007744|PDB:6R00, ECO:0007744|PDB:6TSC

Chain	Residue	Details
A	HIS100
A	ALA130
A	ALA213
A	THR245

---

site_id	SWS_FT_FI4
Number of Residues	1
Details	BINDING: BINDING => ECO:0000269|PubMed:30151425, ECO:0007744|PDB:5N0O, ECO:0007744|PDB:5N0Q, ECO:0007744|PDB:5N0R, ECO:0007744|PDB:5N0S, ECO:0007744|PDB:5N0T, ECO:0007744|PDB:5N0W, ECO:0007744|PDB:5N0X, ECO:0007744|PDB:5N4I, ECO:0007744|PDB:6QZY, ECO:0007744|PDB:6QZZ

Chain	Residue	Details
A	VAL103

---

site_id	SWS_FT_FI5
Number of Residues	1
Details	BINDING: BINDING => ECO:0000269|PubMed:30151425, ECO:0007744|PDB:5N0O, ECO:0007744|PDB:5N0P, ECO:0007744|PDB:5N0R, ECO:0007744|PDB:5N0S, ECO:0007744|PDB:5N0T, ECO:0007744|PDB:5N0U, ECO:0007744|PDB:5N0V, ECO:0007744|PDB:5N0W, ECO:0007744|PDB:5N0X, ECO:0007744|PDB:5N4I, ECO:0007744|PDB:6GEW, ECO:0007744|PDB:6QZY, ECO:0007744|PDB:6QZZ, ECO:0007744|PDB:6R00, ECO:0007744|PDB:6TSC

Chain	Residue	Details
A	GLN172

---

site_id	SWS_FT_FI6
Number of Residues	1
Details	BINDING: BINDING => ECO:0000269|PubMed:30151425, ECO:0007744|PDB:5N0P, ECO:0007744|PDB:5N0Q, ECO:0007744|PDB:6GEW

Chain	Residue	Details
A	SER244

---

site_id	SWS_FT_FI7
Number of Residues	4
Details	MOD_RES: N-methylvaline => ECO:0000269|PubMed:28715095, ECO:0000269|PubMed:30151425, ECO:0000269|PubMed:32491837, ECO:0000269|PubMed:33574430

Chain	Residue	Details
A	MVA401
A	VAL403
A	VAL404
A	VAL406

---

site_id	SWS_FT_FI8
Number of Residues	3
Details	MOD_RES: N-methylglycine => ECO:0000269|PubMed:28715095, ECO:0000269|PubMed:30151425, ECO:0000269|PubMed:32491837, ECO:0000269|PubMed:33574430

Chain	Residue	Details
A	GLY405
A	GLY408
A	GLY411

---

site_id	SWS_FT_FI9
Number of Residues	2
Details	MOD_RES: N-methylisoleucine => ECO:0000269|PubMed:28715095, ECO:0000269|PubMed:30151425, ECO:0000269|PubMed:32491837, ECO:0000269|PubMed:33574430

Chain	Residue	Details
A	ILE407
A	ILE410

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