5N0T

Crystal structure of OphA-DeltaC6 mutant Y76F in complex with SAM

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0008168	molecular_function	methyltransferase activity
A	0016740	molecular_function	transferase activity
A	0032259	biological_process	methylation
B	0008168	molecular_function	methyltransferase activity
B	0016740	molecular_function	transferase activity
B	0032259	biological_process	methylation

Functional Information from PDB Data

site_id	AC1
Number of Residues	20
Details	binding site for residue SAM A 501

Chain	Residue
A	TYR98
A	GLN172
A	TYR211
A	ILE212
A	ALA213
A	GLY242
A	VAL243
A	SER244
A	THR245
B	VAL401
B	ILE402
A	GLY99
B	VAL403
A	HIS100
A	VAL103
A	PHE104
A	VAL105
A	SER129
A	ALA130
A	PHE171

---

site_id	AC2
Number of Residues	22
Details	binding site for residue SAM B 501

Chain	Residue
A	VAL401
A	ILE402
A	VAL403
B	TYR98
B	GLY99
B	HIS100
B	VAL103
B	PHE104
B	VAL105
B	SER129
B	ALA130
B	PHE171
B	GLN172
B	TYR211
B	ILE212
B	ALA213
B	MET215
B	GLY242
B	VAL243
B	SER244
B	THR245
B	HOH705

---

site_id	AC3
Number of Residues	10
Details	binding site for Di-peptide GLY B 174 and CSO B 175

Chain	Residue
B	GLN172
B	VAL173
B	VAL176
B	GLY177
B	ILE178
B	PHE186
B	ASN188
B	GLY241
B	HOH654
B	HOH696

---

site_id	AC4
Number of Residues	10
Details	binding site for Di-peptide CSO B 175 and VAL B 176

Chain	Residue
B	SER153
B	GLN172
B	VAL173
B	GLY174
B	GLY177
B	ILE178
B	PHE186
B	ASN188
B	LYS190
B	HOH631

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	252
Details	Region: {"description":"Clasp domain","evidences":[{"source":"PubMed","id":"28715095","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

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site_id	SWS_FT_FI2
Number of Residues	6
Details	Active site: {"evidences":[{"source":"PubMed","id":"30151425","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PubMed","id":"30151425","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"5N0P","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5N0T","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5N0U","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	8
Details	Binding site: {"evidences":[{"source":"PubMed","id":"30151425","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"5N0O","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5N0P","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5N0Q","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5N0R","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5N0S","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5N0T","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5N0U","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5N0V","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5N0W","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5N0X","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5N4I","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6GEW","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6QZY","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6QZZ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6R00","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6TSC","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PubMed","id":"30151425","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"5N0O","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5N0Q","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5N0R","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5N0S","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5N0T","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5N0W","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5N0X","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5N4I","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6QZY","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6QZZ","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PubMed","id":"30151425","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"5N0O","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5N0P","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5N0R","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5N0S","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5N0T","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5N0U","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5N0V","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5N0W","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5N0X","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5N4I","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6GEW","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6QZY","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6QZZ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6R00","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6TSC","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PubMed","id":"30151425","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"5N0P","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5N0Q","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6GEW","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	8
Details	Modified residue: {"description":"N-methylvaline","evidences":[{"source":"PubMed","id":"28715095","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"30151425","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"32491837","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"33574430","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	2
Details	Modified residue: {"description":"N-methylglycine","evidences":[{"source":"PubMed","id":"28715095","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"30151425","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"32491837","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"33574430","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	2
Details	Modified residue: {"description":"N-methylisoleucine","evidences":[{"source":"PubMed","id":"28715095","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"30151425","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"32491837","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"33574430","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

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