5MY0

KS-MAT DI-DOMAIN OF MOUSE FAS WITH MALONYL-COA

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004315	molecular_function	3-oxoacyl-[acyl-carrier-protein] synthase activity
A	0006633	biological_process	fatty acid biosynthetic process
A	0016740	molecular_function	transferase activity
A	0016746	molecular_function	acyltransferase activity
B	0004315	molecular_function	3-oxoacyl-[acyl-carrier-protein] synthase activity
B	0006633	biological_process	fatty acid biosynthetic process
B	0016740	molecular_function	transferase activity
B	0016746	molecular_function	acyltransferase activity
C	0004315	molecular_function	3-oxoacyl-[acyl-carrier-protein] synthase activity
C	0006633	biological_process	fatty acid biosynthetic process
C	0016740	molecular_function	transferase activity
C	0016746	molecular_function	acyltransferase activity
D	0004315	molecular_function	3-oxoacyl-[acyl-carrier-protein] synthase activity
D	0006633	biological_process	fatty acid biosynthetic process
D	0016740	molecular_function	transferase activity
D	0016746	molecular_function	acyltransferase activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	8
Details	binding site for residue COA A 900

Chain	Residue
A	HIS580
A	K5L581
A	CYS642
A	ASN644
A	THR650
A	LEU766
A	VAL770
A	ARG787

---

site_id	AC2
Number of Residues	10
Details	binding site for residue MLC C 900

Chain	Residue
C	HIS580
C	SER581
C	MET620
C	ASN644
C	PHE671
C	PHE682
C	LEU766
C	ALA769
C	ARG773
C	MET499

---

site_id	AC3
Number of Residues	15
Details	binding site for residue COA D 900

Chain	Residue
D	MET499
D	HIS580
D	K5L581
D	MET620
D	ALA622
D	ASN644
D	ASP647
D	THR648
D	THR650
D	PHE671
D	LYS673
D	VAL675
D	LEU766
D	ALA769
D	ARG773

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Functional Information from PROSITE/UniProt

site_id	PS00606
Number of Residues	17
Details	KS3_1 Ketosynthase family 3 (KS3) active site signature. GPSiaLDtACSSSllAL

Chain	Residue	Details
A	GLY152-LEU168
B	GLY152-LEU168

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	6
Details	ACT_SITE: For beta-ketoacyl synthase activity => ECO:0000255|PROSITE-ProRule:PRU01348

Chain	Residue	Details
B	CYS161
B	HIS293
B	HIS331
C	CYS161
C	HIS293
C	HIS331

---

site_id	SWS_FT_FI2
Number of Residues	2
Details	ACT_SITE: For malonyltransferase activity => ECO:0000255|PROSITE-ProRule:PRU10022

Chain	Residue	Details
B	SER581
C	SER581

---

site_id	SWS_FT_FI3
Number of Residues	6
Details	BINDING: BINDING => ECO:0000269|PubMed:31811668, ECO:0007744|PDB:6ROP

Chain	Residue	Details
B	ASP647
B	PHE671
B	ARG773
C	ASP647
C	PHE671
C	ARG773

---

site_id	SWS_FT_FI4
Number of Residues	4
Details	MOD_RES: N6-acetyllysine => ECO:0007744|PubMed:23806337

Chain	Residue	Details
B	LYS59
B	LYS790
C	LYS59
C	LYS790

---

site_id	SWS_FT_FI5
Number of Residues	4
Details	MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:P49327

Chain	Residue	Details
B	SER63
B	SER207
C	SER63
C	SER207

---

site_id	SWS_FT_FI6
Number of Residues	8
Details	MOD_RES: N6-acetyllysine => ECO:0000250|UniProtKB:P49327

Chain	Residue	Details
B	LYS70
B	LYS298
B	LYS528
B	LYS673
C	LYS70
C	LYS298
C	LYS528
C	LYS673

---

site_id	SWS_FT_FI7
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:21183079

Chain	Residue	Details
B	SER725
C	SER725

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Catalytic Information from CSA

site_id	MCSA1
Number of Residues	4
Details	M-CSA 985

Chain	Residue	Details
C	MET499	transition state stabiliser
C	SER581	nucleophile, proton acceptor
C	LEU582	transition state stabiliser
C	HIS683	activator, proton acceptor, proton donor

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