5M4Z
Crystal structure of the complex of T.spiralis thymidylate synthase with N(4)-hydroxy-2'-deoxycytidine-5'-monophosphate, crystallized in the presence of N(5,10)-methylenetetrahydrofolate
Functional Information from GO Data
Chain | GOid | namespace | contents |
A | 0004146 | molecular_function | dihydrofolate reductase activity |
A | 0004799 | molecular_function | thymidylate synthase activity |
A | 0005739 | cellular_component | mitochondrion |
A | 0005829 | cellular_component | cytosol |
A | 0006231 | biological_process | dTMP biosynthetic process |
A | 0006235 | biological_process | dTTP biosynthetic process |
A | 0008168 | molecular_function | methyltransferase activity |
A | 0009165 | biological_process | nucleotide biosynthetic process |
A | 0016741 | molecular_function | transferase activity, transferring one-carbon groups |
A | 0032259 | biological_process | methylation |
B | 0004146 | molecular_function | dihydrofolate reductase activity |
B | 0004799 | molecular_function | thymidylate synthase activity |
B | 0005739 | cellular_component | mitochondrion |
B | 0005829 | cellular_component | cytosol |
B | 0006231 | biological_process | dTMP biosynthetic process |
B | 0006235 | biological_process | dTTP biosynthetic process |
B | 0008168 | molecular_function | methyltransferase activity |
B | 0009165 | biological_process | nucleotide biosynthetic process |
B | 0016741 | molecular_function | transferase activity, transferring one-carbon groups |
B | 0032259 | biological_process | methylation |
Functional Information from PDB Data
site_id | AC1 |
Number of Residues | 16 |
Details | binding site for residue 7K8 A 401 |
Chain | Residue |
A | TYR129 |
A | HIS250 |
A | TYR252 |
A | HOH502 |
A | HOH553 |
B | ARG169 |
B | ARG170 |
B | HOH578 |
A | CYS189 |
A | HIS190 |
A | GLN208 |
A | ARG209 |
A | SER210 |
A | ALA211 |
A | ASP212 |
A | ASN220 |
site_id | AC2 |
Number of Residues | 8 |
Details | binding site for residue GOL A 402 |
Chain | Residue |
A | GLN29 |
A | THR54 |
A | GLN55 |
A | SER56 |
A | TYR58 |
A | ILE221 |
A | HIS244 |
A | ILE246 |
site_id | AC3 |
Number of Residues | 9 |
Details | binding site for residue GOL A 403 |
Chain | Residue |
A | TYR152 |
A | GLN154 |
A | GLN157 |
A | MET161 |
A | ARG170 |
A | ILE172 |
A | HOH510 |
B | TRP176 |
B | LEU181 |
site_id | AC4 |
Number of Residues | 6 |
Details | binding site for residue GOL A 404 |
Chain | Residue |
A | ALA92 |
A | LYS93 |
A | SER96 |
A | ASP104 |
A | SER107 |
A | SER108 |
site_id | AC5 |
Number of Residues | 9 |
Details | binding site for residue GOL B 402 |
Chain | Residue |
A | TRP176 |
A | LEU181 |
B | TYR152 |
B | GLN154 |
B | GLN157 |
B | MET161 |
B | ARG170 |
B | ILE172 |
B | HOH544 |
site_id | AC6 |
Number of Residues | 7 |
Details | binding site for residue GOL B 403 |
Chain | Residue |
A | ARG57 |
A | TYR196 |
A | SER203 |
A | ILE243 |
A | HOH515 |
B | THR51 |
B | PHE52 |
site_id | AC7 |
Number of Residues | 19 |
Details | binding site for Di-peptide 7K8 B 401 and CYS B 189 |
Chain | Residue |
A | ARG169 |
A | ARG170 |
B | ARG43 |
B | TRP103 |
B | TYR129 |
B | PRO187 |
B | PRO188 |
B | HIS190 |
B | CYS191 |
B | GLN208 |
B | ARG209 |
B | SER210 |
B | ALA211 |
B | ASP212 |
B | ASN220 |
B | HIS250 |
B | TYR252 |
B | HOH502 |
B | HOH568 |
Functional Information from PROSITE/UniProt
site_id | PS00091 |
Number of Residues | 29 |
Details | THYMIDYLATE_SYNTHASE Thymidylate synthase active site. RriImtaWNpcdlekva.....LpPCHcfvQFyV |
Chain | Residue | Details |
A | ARG169-VAL197 |