Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

5M4X

Mutant glyceraldehyde dehydrogenase (F34M+Y399C+S405N) from Thermoplasma acidophilum

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004777	molecular_function	succinate-semialdehyde dehydrogenase (NAD+) activity
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006096	biological_process	glycolytic process
A	0009255	biological_process	Entner-Doudoroff pathway through 6-phosphogluconate
A	0009450	biological_process	gamma-aminobutyric acid catabolic process
A	0016491	molecular_function	oxidoreductase activity
A	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
A	0042803	molecular_function	protein homodimerization activity
A	0043796	molecular_function	glyceraldehyde dehydrogenase (NADP+) activity
A	0051289	biological_process	protein homotetramerization
B	0004777	molecular_function	succinate-semialdehyde dehydrogenase (NAD+) activity
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0006096	biological_process	glycolytic process
B	0009255	biological_process	Entner-Doudoroff pathway through 6-phosphogluconate
B	0009450	biological_process	gamma-aminobutyric acid catabolic process
B	0016491	molecular_function	oxidoreductase activity
B	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
B	0042803	molecular_function	protein homodimerization activity
B	0043796	molecular_function	glyceraldehyde dehydrogenase (NADP+) activity
B	0051289	biological_process	protein homotetramerization
C	0004777	molecular_function	succinate-semialdehyde dehydrogenase (NAD+) activity
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0006096	biological_process	glycolytic process
C	0009255	biological_process	Entner-Doudoroff pathway through 6-phosphogluconate
C	0009450	biological_process	gamma-aminobutyric acid catabolic process
C	0016491	molecular_function	oxidoreductase activity
C	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
C	0042803	molecular_function	protein homodimerization activity
C	0043796	molecular_function	glyceraldehyde dehydrogenase (NADP+) activity
C	0051289	biological_process	protein homotetramerization
D	0004777	molecular_function	succinate-semialdehyde dehydrogenase (NAD+) activity
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0006096	biological_process	glycolytic process
D	0009255	biological_process	Entner-Doudoroff pathway through 6-phosphogluconate
D	0009450	biological_process	gamma-aminobutyric acid catabolic process
D	0016491	molecular_function	oxidoreductase activity
D	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
D	0042803	molecular_function	protein homodimerization activity
D	0043796	molecular_function	glyceraldehyde dehydrogenase (NADP+) activity
D	0051289	biological_process	protein homotetramerization

Functional Information from PROSITE/UniProt

site_id	PS00070
Number of Residues	12
Details	ALDEHYDE_DEHYDR_CYS Aldehyde dehydrogenases cysteine active site. YwNAGQSCIAAE

Chain	Residue	Details
A	TYR274-GLU285

site_id	PS00687
Number of Residues	8
Details	ALDEHYDE_DEHYDR_GLU Aldehyde dehydrogenases glutamic acid active site. LELGGKAP

Chain	Residue	Details
A	LEU246-PRO253

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	ACT_SITE: Proton acceptor => ECO:0000255\|PROSITE-ProRule:PRU10007

Chain	Residue	Details
A	GLU247
B	GLU247
C	GLU247
D	GLU247

site_id	SWS_FT_FI2
Number of Residues	4
Details	ACT_SITE: Proton donor => ECO:0000250\|UniProtKB:P28037

Chain	Residue	Details
A	CYS281
B	CYS281
C	CYS281
D	CYS281

site_id	SWS_FT_FI3
Number of Residues	4
Details	BINDING: BINDING => ECO:0000250\|UniProtKB:P28037

Chain	Residue	Details
A	THR146
B	THR146
C	THR146
D	THR146

site_id	SWS_FT_FI4
Number of Residues	4
Details	BINDING: BINDING => ECO:0000250\|UniProtKB:O57693

Chain	Residue	Details
A	ASN149
B	ASN149
C	ASN149
D	ASN149

site_id	SWS_FT_FI5
Number of Residues	8
Details	BINDING: BINDING => ECO:0000250

Chain	Residue	Details
C	ARG157
C	CYS281
D	ARG157
D	CYS281
A	ARG157
A	CYS281
B	ARG157
B	CYS281

site_id	SWS_FT_FI6
Number of Residues	4
Details	BINDING: BINDING => ECO:0000250\|UniProtKB:Q59931, ECO:0000250\|UniProtKB:Q84DC3

Chain	Residue	Details
A	LYS172
B	LYS172
C	LYS172
D	LYS172

site_id	SWS_FT_FI7
Number of Residues	4
Details	BINDING: BINDING => ECO:0000250\|UniProtKB:Q84DC3

Chain	Residue	Details
A	ARG204
B	ARG204
C	ARG204
D	ARG204

site_id	SWS_FT_FI8
Number of Residues	4
Details	BINDING: BINDING => ECO:0000250\|UniProtKB:Q59931

Chain	Residue	Details
A	GLY225
B	GLY225
C	GLY225
D	GLY225

site_id	SWS_FT_FI9
Number of Residues	4
Details	BINDING: BINDING => ECO:0000250\|UniProtKB:P28037, ECO:0000250\|UniProtKB:Q59931

Chain	Residue	Details
A	GLU381
B	GLU381
C	GLU381
D	GLU381

site_id	SWS_FT_FI10
Number of Residues	4
Details	SITE: Transition state stabilizer => ECO:0000250

Chain	Residue	Details
A	ASN149
B	ASN149
C	ASN149
D	ASN149

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)