5LTM
Crystal structure of phenylalanine ammonia-lyase from Anabaena variabilis (Y78F-C503S-C565S) bound to cinnamate
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003824 | molecular_function | catalytic activity |
| A | 0004397 | molecular_function | histidine ammonia-lyase activity |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0006559 | biological_process | L-phenylalanine catabolic process |
| A | 0009072 | biological_process | aromatic amino acid metabolic process |
| A | 0009698 | biological_process | phenylpropanoid metabolic process |
| A | 0009699 | biological_process | phenylpropanoid biosynthetic process |
| A | 0009800 | biological_process | cinnamic acid biosynthetic process |
| A | 0016829 | molecular_function | lyase activity |
| A | 0016841 | molecular_function | ammonia-lyase activity |
| A | 0045548 | molecular_function | phenylalanine ammonia-lyase activity |
| A | 0051289 | biological_process | protein homotetramerization |
| B | 0003824 | molecular_function | catalytic activity |
| B | 0004397 | molecular_function | histidine ammonia-lyase activity |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0006559 | biological_process | L-phenylalanine catabolic process |
| B | 0009072 | biological_process | aromatic amino acid metabolic process |
| B | 0009698 | biological_process | phenylpropanoid metabolic process |
| B | 0009699 | biological_process | phenylpropanoid biosynthetic process |
| B | 0009800 | biological_process | cinnamic acid biosynthetic process |
| B | 0016829 | molecular_function | lyase activity |
| B | 0016841 | molecular_function | ammonia-lyase activity |
| B | 0045548 | molecular_function | phenylalanine ammonia-lyase activity |
| B | 0051289 | biological_process | protein homotetramerization |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 9 |
| Details | binding site for residue HCI B 601 |
| Chain | Residue |
| A | PHE84 |
| A | GLY85 |
| A | LEU104 |
| A | LEU171 |
| A | ASN451 |
| A | HOH727 |
| B | GLN311 |
| B | TYR314 |
| B | ARG317 |
| site_id | AC2 |
| Number of Residues | 8 |
| Details | binding site for residue HCI A 601 |
| Chain | Residue |
| A | GLN311 |
| A | TYR314 |
| A | ARG317 |
| B | PHE84 |
| B | LEU104 |
| B | LEU171 |
| B | ASN451 |
| B | HOH711 |
| site_id | AC3 |
| Number of Residues | 12 |
| Details | binding site for Di-peptide GLY A 166 and MDO A 168 |
| Chain | Residue |
| A | SER164 |
| A | ILE165 |
| A | ASP170 |
| A | LEU171 |
| A | MET222 |
| A | ASN223 |
| A | GLY224 |
| A | MET228 |
| A | PHE363 |
| A | GLY365 |
| A | SER456 |
| B | TYR314 |
| site_id | AC4 |
| Number of Residues | 16 |
| Details | binding site for Di-peptide MDO A 168 and ASP A 170 |
| Chain | Residue |
| A | SER164 |
| A | ILE165 |
| A | GLY166 |
| A | LEU171 |
| A | VAL172 |
| A | PRO173 |
| A | LEU174 |
| A | MET222 |
| A | ASN223 |
| A | GLY224 |
| A | THR225 |
| A | VAL227 |
| A | MET228 |
| A | PHE363 |
| A | TYR459 |
| B | TYR314 |
Functional Information from PROSITE/UniProt
| site_id | PS00488 |
| Number of Residues | 17 |
| Details | PAL_HISTIDASE Phenylalanine and histidine ammonia-lyases signature. GSIGASGDLvPLSyitG |
| Chain | Residue | Details |
| B | GLY163-LEU181 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 2 |
| Details | ACT_SITE: Proton donor/acceptor => ECO:0000250|UniProtKB:Q68G84 |
| Chain | Residue | Details |
| B | PHE78 | |
| A | PHE78 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 10 |
| Details | BINDING: BINDING => ECO:0000250|UniProtKB:Q68G84 |
| Chain | Residue | Details |
| B | THR225 | |
| A | ASN453 | |
| B | ARG313 | |
| B | LEU319 | |
| B | LEU349 | |
| B | ASN453 | |
| A | THR225 | |
| A | ARG313 | |
| A | LEU319 | |
| A | LEU349 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 4 |
| Details | BINDING: BINDING => ECO:0000250|UniProtKB:P11544 |
| Chain | Residue | Details |
| B | LEU421 | |
| B | PHE450 | |
| A | LEU421 | |
| A | PHE450 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 2 |
| Details | MOD_RES: 2,3-didehydroalanine (Ser) => ECO:0000269|PubMed:17240984 |
| Chain | Residue | Details |
| B | ASP170 | |
| A | ASP170 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 4 |
| Details | CROSSLNK: 5-imidazolinone (Ala-Gly) => ECO:0000269|PubMed:17240984 |
| Chain | Residue | Details |
| B | MDO168 | |
| B | LEU171 | |
| A | MDO168 | |
| A | LEU171 |
