5KZE
N-acetylneuraminate lyase from methicillin-resistant Staphylococcus aureus
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005829 | cellular_component | cytosol |
| A | 0005975 | biological_process | carbohydrate metabolic process |
| A | 0008747 | molecular_function | N-acetylneuraminate lyase activity |
| A | 0016829 | molecular_function | lyase activity |
| A | 0019262 | biological_process | N-acetylneuraminate catabolic process |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005829 | cellular_component | cytosol |
| B | 0005975 | biological_process | carbohydrate metabolic process |
| B | 0008747 | molecular_function | N-acetylneuraminate lyase activity |
| B | 0016829 | molecular_function | lyase activity |
| B | 0019262 | biological_process | N-acetylneuraminate catabolic process |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0005829 | cellular_component | cytosol |
| C | 0005975 | biological_process | carbohydrate metabolic process |
| C | 0008747 | molecular_function | N-acetylneuraminate lyase activity |
| C | 0016829 | molecular_function | lyase activity |
| C | 0019262 | biological_process | N-acetylneuraminate catabolic process |
| D | 0005737 | cellular_component | cytoplasm |
| D | 0005829 | cellular_component | cytosol |
| D | 0005975 | biological_process | carbohydrate metabolic process |
| D | 0008747 | molecular_function | N-acetylneuraminate lyase activity |
| D | 0016829 | molecular_function | lyase activity |
| D | 0019262 | biological_process | N-acetylneuraminate catabolic process |
| E | 0005737 | cellular_component | cytoplasm |
| E | 0005829 | cellular_component | cytosol |
| E | 0005975 | biological_process | carbohydrate metabolic process |
| E | 0008747 | molecular_function | N-acetylneuraminate lyase activity |
| E | 0016829 | molecular_function | lyase activity |
| E | 0019262 | biological_process | N-acetylneuraminate catabolic process |
| F | 0005737 | cellular_component | cytoplasm |
| F | 0005829 | cellular_component | cytosol |
| F | 0005975 | biological_process | carbohydrate metabolic process |
| F | 0008747 | molecular_function | N-acetylneuraminate lyase activity |
| F | 0016829 | molecular_function | lyase activity |
| F | 0019262 | biological_process | N-acetylneuraminate catabolic process |
| G | 0005737 | cellular_component | cytoplasm |
| G | 0005829 | cellular_component | cytosol |
| G | 0005975 | biological_process | carbohydrate metabolic process |
| G | 0008747 | molecular_function | N-acetylneuraminate lyase activity |
| G | 0016829 | molecular_function | lyase activity |
| G | 0019262 | biological_process | N-acetylneuraminate catabolic process |
| H | 0005737 | cellular_component | cytoplasm |
| H | 0005829 | cellular_component | cytosol |
| H | 0005975 | biological_process | carbohydrate metabolic process |
| H | 0008747 | molecular_function | N-acetylneuraminate lyase activity |
| H | 0016829 | molecular_function | lyase activity |
| H | 0019262 | biological_process | N-acetylneuraminate catabolic process |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 6 |
| Details | binding site for residue SO4 A 301 |
| Chain | Residue |
| A | ALA11 |
| A | GLY47 |
| A | SER48 |
| A | SER49 |
| A | TYR137 |
| A | LYS165 |
| site_id | AC2 |
| Number of Residues | 5 |
| Details | binding site for residue GOL A 302 |
| Chain | Residue |
| A | HOH411 |
| A | HOH427 |
| A | ASP191 |
| A | GLU192 |
| A | SER208 |
| site_id | AC3 |
| Number of Residues | 8 |
| Details | binding site for residue SO4 B 301 |
| Chain | Residue |
| B | ALA11 |
| B | GLY47 |
| B | SER48 |
| B | SER49 |
| B | TYR137 |
| B | LYS165 |
| B | HOH402 |
| B | HOH417 |
| site_id | AC4 |
| Number of Residues | 5 |
| Details | binding site for residue GOL B 302 |
| Chain | Residue |
| B | GLY189 |
| B | PHE190 |
| B | ASP191 |
| B | GLU192 |
| B | HOH401 |
| site_id | AC5 |
| Number of Residues | 8 |
| Details | binding site for residue SO4 C 301 |
| Chain | Residue |
| C | ALA11 |
| C | TYR44 |
| C | GLY47 |
| C | SER48 |
| C | SER49 |
| C | TYR137 |
| C | LYS165 |
| C | HOH496 |
| site_id | AC6 |
| Number of Residues | 5 |
| Details | binding site for residue GOL C 302 |
| Chain | Residue |
| C | SER49 |
| C | GLU192 |
| C | SER208 |
| C | ILE251 |
| C | TYR252 |
| site_id | AC7 |
| Number of Residues | 8 |
| Details | binding site for residue SO4 D 301 |
| Chain | Residue |
| D | ALA11 |
| D | GLY47 |
| D | SER48 |
| D | SER49 |
| D | TYR137 |
| D | LYS165 |
| D | HOH421 |
| D | HOH437 |
| site_id | AC8 |
| Number of Residues | 4 |
| Details | binding site for residue GOL D 302 |
| Chain | Residue |
| D | PHE190 |
| D | ASP191 |
| D | GLU192 |
| D | LEU247 |
| site_id | AC9 |
| Number of Residues | 8 |
| Details | binding site for residue SO4 E 301 |
| Chain | Residue |
| E | ALA11 |
| E | GLY47 |
| E | SER48 |
| E | SER49 |
| E | TYR137 |
| E | LYS165 |
| E | HOH417 |
| E | HOH505 |
| site_id | AD1 |
| Number of Residues | 3 |
| Details | binding site for residue GOL E 302 |
| Chain | Residue |
| E | GLY189 |
| E | LEU247 |
| E | ILE251 |
| site_id | AD2 |
| Number of Residues | 7 |
| Details | binding site for residue SO4 F 301 |
| Chain | Residue |
| F | ALA11 |
| F | TYR44 |
| F | GLY47 |
| F | SER48 |
| F | SER49 |
| F | TYR137 |
| F | LYS165 |
| site_id | AD3 |
| Number of Residues | 6 |
| Details | binding site for residue GOL F 302 |
| Chain | Residue |
| F | PHE190 |
| F | SER208 |
| F | HOH401 |
| F | HOH409 |
| F | HOH463 |
| F | HOH507 |
| site_id | AD4 |
| Number of Residues | 8 |
| Details | binding site for residue SO4 G 301 |
| Chain | Residue |
| G | ALA11 |
| G | TYR44 |
| G | GLY47 |
| G | SER48 |
| G | SER49 |
| G | TYR137 |
| G | LYS165 |
| G | HOH413 |
| site_id | AD5 |
| Number of Residues | 3 |
| Details | binding site for residue GOL G 302 |
| Chain | Residue |
| G | GLU192 |
| G | SER208 |
| G | HOH456 |
| site_id | AD6 |
| Number of Residues | 8 |
| Details | binding site for residue SO4 H 301 |
| Chain | Residue |
| H | ALA11 |
| H | TYR44 |
| H | GLY47 |
| H | SER48 |
| H | SER49 |
| H | TYR137 |
| H | LYS165 |
| H | HOH415 |
| site_id | AD7 |
| Number of Residues | 3 |
| Details | binding site for residue GOL H 302 |
| Chain | Residue |
| H | THR167 |
| H | GLY189 |
| H | GLU192 |
Functional Information from PROSITE/UniProt
| site_id | PS00666 |
| Number of Residues | 31 |
| Details | DHDPS_2 Dihydrodipicolinate synthase signature 2. YAIPdlTgvnIsieqfselfnhek.IvGVKYT |
| Chain | Residue | Details |
| A | TYR137-THR167 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 8 |
| Details | ACT_SITE: Proton donor => ECO:0000255|HAMAP-Rule:MF_01237 |
| Chain | Residue | Details |
| A | TYR137 | |
| B | TYR137 | |
| C | TYR137 | |
| D | TYR137 | |
| E | TYR137 | |
| F | TYR137 | |
| G | TYR137 | |
| H | TYR137 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 8 |
| Details | ACT_SITE: Schiff-base intermediate with substrate => ECO:0000255|HAMAP-Rule:MF_01237, ECO:0000269|PubMed:23418011 |
| Chain | Residue | Details |
| A | LYS165 | |
| B | LYS165 | |
| C | LYS165 | |
| D | LYS165 | |
| E | LYS165 | |
| F | LYS165 | |
| G | LYS165 | |
| H | LYS165 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 56 |
| Details | BINDING: BINDING => ECO:0000305|PubMed:27943302, ECO:0007744|PDB:5KZD |
| Chain | Residue | Details |
| A | SER48 | |
| B | ASP191 | |
| B | GLU192 | |
| B | SER208 | |
| C | SER48 | |
| C | SER49 | |
| C | GLY189 | |
| C | ASP191 | |
| C | GLU192 | |
| C | SER208 | |
| D | SER48 | |
| A | SER49 | |
| D | SER49 | |
| D | GLY189 | |
| D | ASP191 | |
| D | GLU192 | |
| D | SER208 | |
| E | SER48 | |
| E | SER49 | |
| E | GLY189 | |
| E | ASP191 | |
| E | GLU192 | |
| A | GLY189 | |
| E | SER208 | |
| F | SER48 | |
| F | SER49 | |
| F | GLY189 | |
| F | ASP191 | |
| F | GLU192 | |
| F | SER208 | |
| G | SER48 | |
| G | SER49 | |
| G | GLY189 | |
| A | ASP191 | |
| G | ASP191 | |
| G | GLU192 | |
| G | SER208 | |
| H | SER48 | |
| H | SER49 | |
| H | GLY189 | |
| H | ASP191 | |
| H | GLU192 | |
| H | SER208 | |
| A | TYR252 | |
| A | GLU192 | |
| B | TYR252 | |
| C | TYR252 | |
| D | TYR252 | |
| E | TYR252 | |
| F | TYR252 | |
| G | TYR252 | |
| H | TYR252 | |
| A | SER208 | |
| B | SER48 | |
| B | SER49 | |
| B | GLY189 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 8 |
| Details | BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_01237 |
| Chain | Residue | Details |
| A | THR167 | |
| B | THR167 | |
| C | THR167 | |
| D | THR167 | |
| E | THR167 | |
| F | THR167 | |
| G | THR167 | |
| H | THR167 |
