5KDP

E491A mutant of choline TMA-lyase

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003824	molecular_function	catalytic activity
A	0005829	cellular_component	cytosol
A	0016829	molecular_function	lyase activity
A	0016840	molecular_function	carbon-nitrogen lyase activity
A	0033265	molecular_function	choline binding
A	0042426	biological_process	choline catabolic process
A	0042803	molecular_function	protein homodimerization activity
A	0120525	molecular_function	choline trimethylamine lyase activity
C	0003824	molecular_function	catalytic activity
C	0005829	cellular_component	cytosol
C	0016829	molecular_function	lyase activity
C	0016840	molecular_function	carbon-nitrogen lyase activity
C	0033265	molecular_function	choline binding
C	0042426	biological_process	choline catabolic process
C	0042803	molecular_function	protein homodimerization activity
C	0120525	molecular_function	choline trimethylamine lyase activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	6
Details	binding site for residue NA A 901

Chain	Residue
A	SER746
A	MET748
A	HOH1071
A	HOH1370
A	HOH1525
A	HOH1537

---

site_id	AC2
Number of Residues	5
Details	binding site for residue MLI A 902

Chain	Residue
A	HOH1007
A	HOH1283
A	GLU250
A	ARG572
A	LEU689

---

site_id	AC3
Number of Residues	7
Details	binding site for residue MLI A 903

Chain	Residue
A	ARG238
A	LYS259
A	THR633
A	GLN636
A	HOH1067
A	HOH1077
A	HOH1534

---

site_id	AC4
Number of Residues	7
Details	binding site for residue MLI A 904

Chain	Residue
A	LYS102
A	ARG105
A	TYR106
A	HOH1207
A	HOH1247
A	HOH1387
A	HOH1538

---

site_id	AC5
Number of Residues	9
Details	binding site for residue MLI A 905

Chain	Residue
A	GLY49
A	SER50
A	HIS51
A	ARG290
A	HOH1013
A	HOH1056
A	HOH1109
A	HOH1212
A	HOH1708

---

site_id	AC6
Number of Residues	6
Details	binding site for residue NA C 901

Chain	Residue
A	HOH1519
C	SER746
C	MET748
C	HOH1352
C	HOH1386
C	HOH1525

---

site_id	AC7
Number of Residues	4
Details	binding site for residue MLI C 902

Chain	Residue
C	ARG572
C	LEU689
C	HOH1005
C	HOH1366

---

site_id	AC8
Number of Residues	5
Details	binding site for residue MLI C 903

Chain	Residue
C	HIS519
C	ASP535
C	LEU536
C	SER537
C	ALA635

---

Functional Information from PROSITE/UniProt

site_id	PS00850
Number of Residues	9
Details	GLY_RADICAL_1 Glycine radical domain signature. VvRVAGYSA

Chain	Residue	Details
A	VAL816-ALA824

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1316
Details	Domain: {"description":"PFL","evidences":[{"source":"PROSITE-ProRule","id":"PRU00887","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	242
Details	Domain: {"description":"Glycine radical","evidences":[{"source":"PROSITE-ProRule","id":"PRU00493","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	Active site: {"description":"Cysteine radical intermediate","evidences":[{"source":"HAMAP-Rule","id":"MF_02058","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"23151509","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"24854437","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	Active site: {"description":"Proton acceptor","evidences":[{"source":"HAMAP-Rule","id":"MF_02058","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"24854437","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	2
Details	Modified residue: {"description":"Glycine radical","evidences":[{"source":"HAMAP-Rule","id":"MF_02058","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"23151509","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"24854437","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---