5JYC
Crystal structure of the E153Q mutant of the CFTR inhibitory factor Cif containing the adducted 14,15-EET hydrolysis intermediate
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004301 | molecular_function | epoxide hydrolase activity |
| A | 0005576 | cellular_component | extracellular region |
| A | 0043657 | cellular_component | host cell |
| B | 0004301 | molecular_function | epoxide hydrolase activity |
| B | 0005576 | cellular_component | extracellular region |
| B | 0043657 | cellular_component | host cell |
| C | 0004301 | molecular_function | epoxide hydrolase activity |
| C | 0005576 | cellular_component | extracellular region |
| C | 0043657 | cellular_component | host cell |
| D | 0004301 | molecular_function | epoxide hydrolase activity |
| D | 0005576 | cellular_component | extracellular region |
| D | 0043657 | cellular_component | host cell |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 13 |
| Details | binding site for residue EET A 401 |
| Chain | Residue |
| A | ASP129 |
| A | MET272 |
| A | HIS297 |
| A | TRP298 |
| A | HOH507 |
| A | GLN153 |
| A | PHE164 |
| A | PRO165 |
| A | VAL175 |
| A | HIS177 |
| A | PHE178 |
| A | HIS207 |
| A | TYR239 |
| site_id | AC2 |
| Number of Residues | 17 |
| Details | binding site for Di-peptide EET B 401 and ASP B 129 |
| Chain | Residue |
| B | PHE63 |
| B | HIS128 |
| B | ILE130 |
| B | GLY131 |
| B | ILE132 |
| B | MET152 |
| B | GLN153 |
| B | ALA154 |
| B | VAL175 |
| B | HIS177 |
| B | PHE178 |
| B | PHE203 |
| B | TYR239 |
| B | MET272 |
| B | PHE275 |
| B | HIS297 |
| B | HOH619 |
| site_id | AC3 |
| Number of Residues | 19 |
| Details | binding site for Di-peptide EET C 401 and ASP C 129 |
| Chain | Residue |
| C | PHE63 |
| C | HIS128 |
| C | ILE130 |
| C | GLY131 |
| C | ILE132 |
| C | MET152 |
| C | GLN153 |
| C | ALA154 |
| C | PHE164 |
| C | PRO165 |
| C | VAL175 |
| C | HIS177 |
| C | PHE178 |
| C | HIS207 |
| C | TYR239 |
| C | MET272 |
| C | HIS297 |
| C | TRP298 |
| C | HOH594 |
| site_id | AC4 |
| Number of Residues | 19 |
| Details | binding site for Di-peptide EET D 401 and ASP D 129 |
| Chain | Residue |
| D | PHE63 |
| D | HIS128 |
| D | ILE130 |
| D | GLY131 |
| D | ILE132 |
| D | MET152 |
| D | GLN153 |
| D | ALA154 |
| D | PHE164 |
| D | VAL175 |
| D | HIS177 |
| D | PHE178 |
| D | PHE203 |
| D | HIS207 |
| D | TYR239 |
| D | MET272 |
| D | HIS297 |
| D | HOH593 |
| D | HOH623 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 440 |
| Details | Domain: {"description":"AB hydrolase-1","evidences":[{"evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 4 |
| Details | Active site: {"description":"Nucleophile; hydroxyalkyl-enzyme intermediate","evidences":[{"source":"PubMed","id":"26752215","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"27980032","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"28392259","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"20118260","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"21933119","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"26136396","evidenceCode":"ECO:0000305"},{"source":"PDB","id":"4DNF","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4DNO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5JYC","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 4 |
| Details | Active site: {"evidences":[{"source":"PubMed","id":"20118260","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"21933119","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"26136396","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"26752215","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 4 |
| Details | Active site: {"description":"Proton acceptor","evidences":[{"source":"PubMed","id":"20118260","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"21933119","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"26136396","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"26752215","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 8 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"26752215","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"27980032","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"28392259","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"4DNF","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4DNO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4EHB","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4EUS","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5JYC","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
