5ICF

Crystal structure of (S)-norcoclaurine 6-O-methyltransferase with S-adenosyl-L-homocysteine and sanguinarine

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0008168	molecular_function	methyltransferase activity
A	0008171	molecular_function	O-methyltransferase activity
A	0009820	biological_process	alkaloid metabolic process
A	0016740	molecular_function	transferase activity
A	0030786	molecular_function	(RS)-norcoclaurine 6-O-methyltransferase activity
A	0032259	biological_process	methylation
A	0046983	molecular_function	protein dimerization activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	21
Details	binding site for residue SAH A 401

Chain	Residue
A	TRP149
A	LYS252
A	CYS253
A	ILE254
A	ASP257
A	TRP258
A	HOH525
A	HOH574
A	HOH598
A	HOH601
A	HOH609
A	MET166
A	HOH610
A	HOH622
A	THR170
A	GLY195
A	ASP218
A	LEU219
A	VAL222
A	ASP238
A	MET239

---

site_id	AC2
Number of Residues	12
Details	binding site for residue SAU A 402

Chain	Residue
A	TRP16
A	TYR20
A	THR113
A	PHE119
A	PHE140
A	PHE162
A	GLY165
A	MET166
A	ASP169
A	ASP306
A	ASN310
A	EDO412

---

site_id	AC3
Number of Residues	4
Details	binding site for residue K A 403

Chain	Residue
A	HIS124
A	HIS124
A	HOH605
A	HOH605

---

site_id	AC4
Number of Residues	3
Details	binding site for residue K A 404

Chain	Residue
A	ASP289
A	THR297
A	ARG300

---

site_id	AC5
Number of Residues	4
Details	binding site for residue NA A 405

Chain	Residue
A	GLN12
A	TRP16
A	TYR296
A	HOH683

---

site_id	AC6
Number of Residues	7
Details	binding site for residue EDO A 406

Chain	Residue
A	ASN38
A	HIS41
A	ASN42
A	VAL100
A	LYS101
A	TYR242
A	HOH510

---

site_id	AC7
Number of Residues	5
Details	binding site for residue EDO A 407

Chain	Residue
A	ILE40
A	GLY44
A	SER45
A	GLY91
A	LEU92

---

site_id	AC8
Number of Residues	3
Details	binding site for residue EDO A 408

Chain	Residue
A	HIS75
A	MET76
A	LYS298

---

site_id	AC9
Number of Residues	3
Details	binding site for residue EDO A 409

Chain	Residue
A	PRO220
A	HIS221
A	HOH541

---

site_id	AD1
Number of Residues	4
Details	binding site for residue EDO A 410

Chain	Residue
A	HIS55
A	ASN233
A	SER234
A	ILE235

---

site_id	AD2
Number of Residues	8
Details	binding site for residue EDO A 411

Chain	Residue
A	SER10
A	HIS155
A	HIS294
A	PRO295
A	HOH504
A	HOH513
A	HOH552
A	HOH615

---

site_id	AD3
Number of Residues	7
Details	binding site for residue EDO A 412

Chain	Residue
A	PHE162
A	MET166
A	ASP306
A	MET307
A	THR311
A	SAU402
A	HOH536

---

site_id	AD4
Number of Residues	3
Details	binding site for residue EDO A 413

Chain	Residue
A	LYS117
A	LYS117
A	ASP118

---

site_id	AD5
Number of Residues	9
Details	binding site for residue EDO A 414

Chain	Residue
A	HIS55
A	LEU56
A	THR190
A	LYS213
A	THR215
A	ASN233
A	ASN245
A	ALA246
A	HOH501

---

site_id	AD6
Number of Residues	5
Details	binding site for residue EDO A 415

Chain	Residue
A	TRP258
A	ASP259
A	GLU262
A	THR311
A	GLY312

---

site_id	AD7
Number of Residues	3
Details	binding site for residue EDO A 416

Chain	Residue
A	HOH515
A	HOH667
A	ASP116

---

site_id	AD8
Number of Residues	4
Details	binding site for residue EDO A 417

Chain	Residue
A	ASP116
A	LYS117
A	ASP118
A	LYS158

---

site_id	AD9
Number of Residues	4
Details	binding site for residue EDO A 418

Chain	Residue
A	ASP150
A	GLU154
A	HIS294
A	DMS420

---

site_id	AE1
Number of Residues	6
Details	binding site for residue DMS A 419

Chain	Residue
A	ASN5
A	PRO156
A	ASN159
A	GLN160
A	TYR350
A	HOH647

---

site_id	AE2
Number of Residues	10
Details	binding site for residue DMS A 420

Chain	Residue
A	LEU9
A	GLU154
A	TYR296
A	ARG300
A	HIS337
A	SER339
A	ALA340
A	EDO418
A	HOH511
A	HOH565

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	Active site: {"description":"Proton acceptor","evidences":[{"source":"PROSITE-ProRule","id":"PRU01020","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	5
Details	Binding site: {"evidences":[{"source":"PubMed","id":"27232113","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"5ICC","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5ICE","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5ICF","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	6
Details	Binding site: {"evidences":[{"source":"PubMed","id":"27232113","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"5ICE","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	1
Details	Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU01020","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"27232113","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"5ICC","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5ICE","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5ICF","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---