5I3D
Sulfolobus solfataricus beta-glycosidase - E387Y mutant
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004553 | molecular_function | hydrolase activity, hydrolyzing O-glycosyl compounds |
| A | 0004565 | molecular_function | beta-galactosidase activity |
| A | 0005975 | biological_process | carbohydrate metabolic process |
| A | 0008422 | molecular_function | beta-glucosidase activity |
| A | 0016798 | molecular_function | hydrolase activity, acting on glycosyl bonds |
| B | 0004553 | molecular_function | hydrolase activity, hydrolyzing O-glycosyl compounds |
| B | 0004565 | molecular_function | beta-galactosidase activity |
| B | 0005975 | biological_process | carbohydrate metabolic process |
| B | 0008422 | molecular_function | beta-glucosidase activity |
| B | 0016798 | molecular_function | hydrolase activity, acting on glycosyl bonds |
| C | 0004553 | molecular_function | hydrolase activity, hydrolyzing O-glycosyl compounds |
| C | 0004565 | molecular_function | beta-galactosidase activity |
| C | 0005975 | biological_process | carbohydrate metabolic process |
| C | 0008422 | molecular_function | beta-glucosidase activity |
| C | 0016798 | molecular_function | hydrolase activity, acting on glycosyl bonds |
| D | 0004553 | molecular_function | hydrolase activity, hydrolyzing O-glycosyl compounds |
| D | 0004565 | molecular_function | beta-galactosidase activity |
| D | 0005975 | biological_process | carbohydrate metabolic process |
| D | 0008422 | molecular_function | beta-glucosidase activity |
| D | 0016798 | molecular_function | hydrolase activity, acting on glycosyl bonds |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 9 |
| Details | binding site for residue PE8 A 501 |
| Chain | Residue |
| A | GLU112 |
| A | ASN113 |
| A | LYS116 |
| A | ASP119 |
| A | HOH749 |
| A | HOH805 |
| B | ILE413 |
| B | ASN414 |
| B | GLY416 |
| site_id | AC2 |
| Number of Residues | 6 |
| Details | binding site for residue ACT A 502 |
| Chain | Residue |
| A | GLU206 |
| A | TRP361 |
| A | ACT503 |
| A | HOH606 |
| A | HOH667 |
| A | HOH732 |
| site_id | AC3 |
| Number of Residues | 5 |
| Details | binding site for residue ACT A 503 |
| Chain | Residue |
| A | PHE359 |
| A | GLU432 |
| A | TRP433 |
| A | ACT502 |
| A | HOH609 |
| site_id | AC4 |
| Number of Residues | 7 |
| Details | binding site for residue ACT A 504 |
| Chain | Residue |
| A | TRP84 |
| A | PHE88 |
| A | LEU92 |
| A | LEU118 |
| A | GLU182 |
| A | ARG185 |
| A | PHE186 |
| site_id | AC5 |
| Number of Residues | 6 |
| Details | binding site for residue ACT B 501 |
| Chain | Residue |
| B | GLU206 |
| B | TRP361 |
| B | ACT502 |
| B | HOH608 |
| B | HOH640 |
| B | HOH641 |
| site_id | AC6 |
| Number of Residues | 4 |
| Details | binding site for residue ACT B 502 |
| Chain | Residue |
| B | PHE359 |
| B | GLU432 |
| B | ACT501 |
| B | HOH731 |
| site_id | AC7 |
| Number of Residues | 5 |
| Details | binding site for residue ACT B 503 |
| Chain | Residue |
| B | TRP84 |
| B | PHE88 |
| B | LEU92 |
| B | GLU182 |
| B | PHE186 |
| site_id | AC8 |
| Number of Residues | 5 |
| Details | binding site for residue ACT B 504 |
| Chain | Residue |
| B | GLU279 |
| B | GLU282 |
| B | TRP287 |
| B | LYS374 |
| B | HOH719 |
| site_id | AC9 |
| Number of Residues | 7 |
| Details | binding site for residue ACT C 501 |
| Chain | Residue |
| C | GLU206 |
| C | TRP361 |
| C | ACT502 |
| C | HOH638 |
| C | HOH639 |
| C | HOH651 |
| C | HOH679 |
| site_id | AD1 |
| Number of Residues | 4 |
| Details | binding site for residue ACT C 502 |
| Chain | Residue |
| C | PHE359 |
| C | GLU432 |
| C | ACT501 |
| C | HOH612 |
| site_id | AD2 |
| Number of Residues | 6 |
| Details | binding site for residue ACT C 503 |
| Chain | Residue |
| C | TRP84 |
| C | PHE88 |
| C | LEU92 |
| C | TRP156 |
| C | GLU182 |
| C | PHE186 |
| site_id | AD3 |
| Number of Residues | 4 |
| Details | binding site for residue ACT D 501 |
| Chain | Residue |
| A | ARG463 |
| D | GLU464 |
| D | ASN468 |
| D | ACT502 |
| site_id | AD4 |
| Number of Residues | 3 |
| Details | binding site for residue ACT D 502 |
| Chain | Residue |
| A | ARG463 |
| D | GLU474 |
| D | ACT501 |
| site_id | AD5 |
| Number of Residues | 7 |
| Details | binding site for residue ACT D 503 |
| Chain | Residue |
| D | GLU206 |
| D | TYR322 |
| D | TRP361 |
| D | ACT504 |
| D | HOH609 |
| D | HOH647 |
| D | HOH726 |
| site_id | AD6 |
| Number of Residues | 4 |
| Details | binding site for residue ACT D 504 |
| Chain | Residue |
| D | PHE359 |
| D | GLU432 |
| D | ACT503 |
| D | HOH644 |
| site_id | AD7 |
| Number of Residues | 6 |
| Details | binding site for residue ACT D 505 |
| Chain | Residue |
| D | TRP84 |
| D | PHE88 |
| D | LEU92 |
| D | LEU118 |
| D | GLU182 |
| D | PHE186 |
Functional Information from PROSITE/UniProt
| site_id | PS00653 |
| Number of Residues | 15 |
| Details | GLYCOSYL_HYDROL_F1_2 Glycosyl hydrolases family 1 N-terminal signature. FrFGwSqAGFQsEmG |
| Chain | Residue | Details |
| A | PHE8-GLY22 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 4 |
| Details | ACT_SITE: Proton donor => ECO:0000255 |
| Chain | Residue | Details |
| A | GLU206 | |
| B | GLU206 | |
| C | GLU206 | |
| D | GLU206 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 4 |
| Details | ACT_SITE: Nucleophile => ECO:0000255|PROSITE-ProRule:PRU10055 |
| Chain | Residue | Details |
| A | TYR387 | |
| B | TYR387 | |
| C | TYR387 | |
| D | TYR387 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 16 |
| Details | SITE: Not N6-methylated |
| Chain | Residue | Details |
| A | LYS76 | |
| C | LYS102 | |
| C | LYS124 | |
| C | LYS138 | |
| D | LYS76 | |
| D | LYS102 | |
| D | LYS124 | |
| D | LYS138 | |
| A | LYS102 | |
| A | LYS124 | |
| A | LYS138 | |
| B | LYS76 | |
| B | LYS102 | |
| B | LYS124 | |
| B | LYS138 | |
| C | LYS76 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 12 |
| Details | MOD_RES: N6-methyllysine; partial => ECO:0000269|PubMed:14660666 |
| Chain | Residue | Details |
| A | LYS116 | |
| D | LYS116 | |
| D | LYS273 | |
| D | LYS311 | |
| A | LYS273 | |
| A | LYS311 | |
| B | LYS116 | |
| B | LYS273 | |
| B | LYS311 | |
| C | LYS116 | |
| C | LYS273 | |
| C | LYS311 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 8 |
| Details | MOD_RES: N6-methyllysine => ECO:0000269|PubMed:14660666 |
| Chain | Residue | Details |
| A | LYS135 | |
| A | LYS332 | |
| B | LYS135 | |
| B | LYS332 | |
| C | LYS135 | |
| C | LYS332 | |
| D | LYS135 | |
| D | LYS332 |
