5HT0

Crystal structure of an Antibiotic_NAT family aminoglycoside acetyltransferase HMB0038 from an uncultured soil metagenomic sample in complex with coenzyme A

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0016740	molecular_function	transferase activity
A	0016746	molecular_function	acyltransferase activity
A	0046353	molecular_function	aminoglycoside 3-N-acetyltransferase activity
A	0046677	biological_process	response to antibiotic
B	0016740	molecular_function	transferase activity
B	0016746	molecular_function	acyltransferase activity
B	0046353	molecular_function	aminoglycoside 3-N-acetyltransferase activity
B	0046677	biological_process	response to antibiotic
C	0016740	molecular_function	transferase activity
C	0016746	molecular_function	acyltransferase activity
C	0046353	molecular_function	aminoglycoside 3-N-acetyltransferase activity
C	0046677	biological_process	response to antibiotic
D	0016740	molecular_function	transferase activity
D	0016746	molecular_function	acyltransferase activity
D	0046353	molecular_function	aminoglycoside 3-N-acetyltransferase activity
D	0046677	biological_process	response to antibiotic
E	0016740	molecular_function	transferase activity
E	0016746	molecular_function	acyltransferase activity
E	0046353	molecular_function	aminoglycoside 3-N-acetyltransferase activity
E	0046677	biological_process	response to antibiotic
F	0016740	molecular_function	transferase activity
F	0016746	molecular_function	acyltransferase activity
F	0046353	molecular_function	aminoglycoside 3-N-acetyltransferase activity
F	0046677	biological_process	response to antibiotic

Functional Information from PDB Data

site_id	AC1
Number of Residues	18
Details	binding site for residue COA A 301

Chain	Residue
A	HIS29
A	GLY96
A	PHE97
A	PRO160
A	THR163
A	THR165
A	HOH402
A	HOH408
B	GLN66
B	ARG93
A	ALA30
A	ALA31
A	LEU32
A	ARG33
A	PRO42
A	TYR62
A	ASP94
A	ASN95

---

site_id	AC2
Number of Residues	2
Details	binding site for residue SO4 A 302

Chain	Residue
A	ASP131
A	HOH409

---

site_id	AC3
Number of Residues	4
Details	binding site for residue SO4 A 303

Chain	Residue
A	ALA133
A	LEU134
A	ASP135
A	ARG141

---

site_id	AC4
Number of Residues	2
Details	binding site for residue SO4 A 304

Chain	Residue
A	ALA21
A	ASP22

---

site_id	AC5
Number of Residues	13
Details	binding site for residue COA B 301

Chain	Residue
A	GLN66
A	ARG93
B	HIS29
B	ALA30
B	ALA31
B	LEU32
B	ARG33
B	PRO42
B	TYR62
B	ASN95
B	GLY96
B	PHE97
B	ALA159

---

site_id	AC6
Number of Residues	1
Details	binding site for residue SO4 B 302

Chain	Residue
B	ASP131

---

site_id	AC7
Number of Residues	4
Details	binding site for residue SO4 B 303

Chain	Residue
B	ALA133
B	LEU134
B	ASP135
B	ARG141

---

site_id	AC8
Number of Residues	2
Details	binding site for residue SO4 B 304

Chain	Residue
B	ALA21
B	ASP22

---

site_id	AC9
Number of Residues	2
Details	binding site for residue SO4 B 305

Chain	Residue
B	ARG8
F	ARG260

---

site_id	AD1
Number of Residues	1
Details	binding site for residue SO4 B 306

Chain	Residue
B	ARG78

---

site_id	AD2
Number of Residues	13
Details	binding site for residue COA C 301

Chain	Residue
C	HIS29
C	ALA30
C	ALA31
C	LEU32
C	ARG33
C	PRO42
C	ASN95
C	GLY96
C	PHE97
C	ALA159
C	THR163
D	GLN66
D	ARG93

---

site_id	AD3
Number of Residues	2
Details	binding site for residue SO4 C 302

Chain	Residue
C	ALA112
C	ASP131

---

site_id	AD4
Number of Residues	1
Details	binding site for residue SO4 C 303

Chain	Residue
C	ARG8

---

site_id	AD5
Number of Residues	2
Details	binding site for residue SO4 C 304

Chain	Residue
C	ARG78
C	TRP196

---

site_id	AD6
Number of Residues	13
Details	binding site for residue COA D 301

Chain	Residue
C	GLN66
C	ARG93
D	HIS29
D	ALA30
D	ALA31
D	LEU32
D	ARG33
D	PRO42
D	ASN95
D	PHE97
D	PRO160
D	HOH408
D	HOH417

---

site_id	AD7
Number of Residues	1
Details	binding site for residue SO4 D 302

Chain	Residue
D	ASP131

---

site_id	AD8
Number of Residues	2
Details	binding site for residue SO4 D 303

Chain	Residue
D	ARG8
D	HOH411

---

site_id	AD9
Number of Residues	2
Details	binding site for residue SO4 D 304

Chain	Residue
D	ALA21
D	ASP22

---

site_id	AE1
Number of Residues	15
Details	binding site for residue COA E 301

Chain	Residue
F	ARG93
E	HIS29
E	ALA30
E	ALA31
E	LEU32
E	ARG33
E	PRO42
E	TYR62
E	ASN95
E	PHE97
E	ALA159
E	THR163
E	THR165
E	HOH406
F	GLN66

---

site_id	AE2
Number of Residues	2
Details	binding site for residue SO4 E 302

Chain	Residue
E	ASP131
E	HIS132

---

site_id	AE3
Number of Residues	1
Details	binding site for residue SO4 E 303

Chain	Residue
E	ARG8

---

site_id	AE4
Number of Residues	2
Details	binding site for residue SO4 E 304

Chain	Residue
E	ARG78
E	TRP196

---

site_id	AE5
Number of Residues	13
Details	binding site for residue COA F 301

Chain	Residue
E	GLN66
E	ARG93
F	HIS29
F	ALA30
F	ALA31
F	LEU32
F	ARG33
F	PRO42
F	ASN95
F	GLY96
F	PHE97
F	PRO160
F	THR163

---

site_id	AE6
Number of Residues	3
Details	binding site for residue SO4 F 302

Chain	Residue
F	ALA130
F	ASP131
F	HOH424

---

site_id	AE7
Number of Residues	1
Details	binding site for residue SO4 F 303

Chain	Residue
F	ARG197

---

site_id	AE8
Number of Residues	1
Details	binding site for residue SO4 F 304

Chain	Residue
F	ARG8

---