Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

5HSA

Alcohol Oxidase AOX1 from Pichia Pastoris

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005782	cellular_component	peroxisomal matrix
A	0015945	biological_process	methanol metabolic process
A	0016491	molecular_function	oxidoreductase activity
A	0016614	molecular_function	oxidoreductase activity, acting on CH-OH group of donors
A	0046188	biological_process	methane catabolic process
A	0047639	molecular_function	alcohol oxidase activity
A	0050660	molecular_function	flavin adenine dinucleotide binding
B	0005782	cellular_component	peroxisomal matrix
B	0015945	biological_process	methanol metabolic process
B	0016491	molecular_function	oxidoreductase activity
B	0016614	molecular_function	oxidoreductase activity, acting on CH-OH group of donors
B	0046188	biological_process	methane catabolic process
B	0047639	molecular_function	alcohol oxidase activity
B	0050660	molecular_function	flavin adenine dinucleotide binding
C	0005782	cellular_component	peroxisomal matrix
C	0015945	biological_process	methanol metabolic process
C	0016491	molecular_function	oxidoreductase activity
C	0016614	molecular_function	oxidoreductase activity, acting on CH-OH group of donors
C	0046188	biological_process	methane catabolic process
C	0047639	molecular_function	alcohol oxidase activity
C	0050660	molecular_function	flavin adenine dinucleotide binding
D	0005782	cellular_component	peroxisomal matrix
D	0015945	biological_process	methanol metabolic process
D	0016491	molecular_function	oxidoreductase activity
D	0016614	molecular_function	oxidoreductase activity, acting on CH-OH group of donors
D	0046188	biological_process	methane catabolic process
D	0047639	molecular_function	alcohol oxidase activity
D	0050660	molecular_function	flavin adenine dinucleotide binding
E	0005782	cellular_component	peroxisomal matrix
E	0015945	biological_process	methanol metabolic process
E	0016491	molecular_function	oxidoreductase activity
E	0016614	molecular_function	oxidoreductase activity, acting on CH-OH group of donors
E	0046188	biological_process	methane catabolic process
E	0047639	molecular_function	alcohol oxidase activity
E	0050660	molecular_function	flavin adenine dinucleotide binding
F	0005782	cellular_component	peroxisomal matrix
F	0015945	biological_process	methanol metabolic process
F	0016491	molecular_function	oxidoreductase activity
F	0016614	molecular_function	oxidoreductase activity, acting on CH-OH group of donors
F	0046188	biological_process	methane catabolic process
F	0047639	molecular_function	alcohol oxidase activity
F	0050660	molecular_function	flavin adenine dinucleotide binding
G	0005782	cellular_component	peroxisomal matrix
G	0015945	biological_process	methanol metabolic process
G	0016491	molecular_function	oxidoreductase activity
G	0016614	molecular_function	oxidoreductase activity, acting on CH-OH group of donors
G	0046188	biological_process	methane catabolic process
G	0047639	molecular_function	alcohol oxidase activity
G	0050660	molecular_function	flavin adenine dinucleotide binding
H	0005782	cellular_component	peroxisomal matrix
H	0015945	biological_process	methanol metabolic process
H	0016491	molecular_function	oxidoreductase activity
H	0016614	molecular_function	oxidoreductase activity, acting on CH-OH group of donors
H	0046188	biological_process	methane catabolic process
H	0047639	molecular_function	alcohol oxidase activity
H	0050660	molecular_function	flavin adenine dinucleotide binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	36
Details	binding site for residue FAS A 701

Chain	Residue
A	GLY13
A	GLY92
A	GLY93
A	ASN97
A	PHE98
A	MET99
A	MET100
A	THR225
A	LYS226
A	VAL227
A	CYS268
A	GLY15
A	GLY269
A	SER272
A	TRP566
A	ASP605
A	LEU606
A	ASN616
A	THR617
A	TYR618
A	HOH848
A	HOH890
A	SER16
A	HOH892
A	HOH920
A	HOH937
A	HOH940
A	HOH964
A	HOH965
A	HOH1012
A	SER17
A	GLU38
A	ALA39
A	ALA87
A	ASN88
A	VAL89

site_id	AC2
Number of Residues	12
Details	binding site for residue P6G A 702

Chain	Residue
A	LYS407
A	THR478
A	SER479
A	GLU494
A	TRP518
A	THR519
E	LYS407
E	THR478
E	SER479
E	GLU494
E	TRP518
E	THR519

site_id	AC3
Number of Residues	6
Details	binding site for residue PGE A 703

Chain	Residue
A	TRP48
A	GLN336
A	PHE340
A	HOH802
E	PRO506
E	LEU507

site_id	AC4
Number of Residues	11
Details	binding site for residue PO4 A 704

Chain	Residue
A	ASP341
A	HOH801
A	HOH803
A	HOH858
A	HOH908
B	ASP341
B	HOH850
C	ASP341
C	HOH1098
D	HOH804
D	HOH963

site_id	AC5
Number of Residues	5
Details	binding site for residue CA A 705

Chain	Residue
A	GLU382
A	HOH1005
A	HOH1075
A	HOH1107
A	HOH1109

site_id	AC6
Number of Residues	35
Details	binding site for residue FAS B 701

Chain	Residue
B	HOH810
B	HOH817
B	HOH820
B	HOH846
B	HOH886
B	HOH911
B	GLY13
B	GLY15
B	SER16
B	SER17
B	GLU38
B	ALA39
B	ALA87
B	VAL89
B	GLY92
B	GLY93
B	ASN97
B	PHE98
B	MET99
B	MET100
B	TRP193
B	THR225
B	LYS226
B	VAL227
B	CYS268
B	GLY269
B	SER272
B	TRP566
B	HIS567
B	ASP605
B	LEU606
B	ASN616
B	THR617
B	TYR618
B	HOH807

site_id	AC7
Number of Residues	10
Details	binding site for residue P6G B 702

Chain	Residue
A	ASN42
A	LEU44
A	LEU221
A	CYS223
A	ASN224
B	GLN161
B	LEU164
B	GLU168
B	TYR174
B	HIS189

site_id	AC8
Number of Residues	13
Details	binding site for residue P6G B 703

Chain	Residue
B	LYS407
B	THR478
B	SER479
B	GLU494
B	TRP518
B	THR519
G	LYS407
G	THR478
G	SER479
G	LEU483
G	GLU494
G	TRP518
G	THR519

site_id	AC9
Number of Residues	6
Details	binding site for residue PGE B 704

Chain	Residue
B	TRP48
B	GLN336
B	PHE340
B	HOH909
G	PRO506
G	LEU507

site_id	AD1
Number of Residues	7
Details	binding site for residue PGE B 705

Chain	Residue
B	PRO506
B	LEU507
B	LEU509
B	HOH877
G	TRP48
G	GLN336
G	PHE340

site_id	AD2
Number of Residues	4
Details	binding site for residue CA B 706

Chain	Residue
B	GLU382
B	HOH941
B	HOH984
B	HOH1007

site_id	AD3
Number of Residues	37
Details	binding site for residue FAS C 701

Chain	Residue
C	GLY13
C	GLY15
C	SER16
C	SER17
C	GLU38
C	ALA39
C	ALA87
C	ASN88
C	VAL89
C	GLY92
C	GLY93
C	ILE96
C	ASN97
C	PHE98
C	MET99
C	MET100
C	THR225
C	LYS226
C	VAL227
C	CYS268
C	SER272
C	TRP566
C	ASP605
C	LEU606
C	ASN616
C	THR617
C	TYR618
C	HOH808
C	HOH841
C	HOH869
C	HOH874
C	HOH883
C	HOH928
C	HOH933
C	HOH943
C	HOH985
C	HOH1015

site_id	AD4
Number of Residues	12
Details	binding site for residue P6G C 702

Chain	Residue
A	CYS160
A	GLN161
A	LEU164
A	GLU168
A	TYR174
A	HIS189
C	ASN42
C	LEU44
C	ASN46
C	LEU221
C	CYS223
C	ASN224

site_id	AD5
Number of Residues	8
Details	binding site for residue P6G C 703

Chain	Residue
C	GLN161
C	LEU164
C	TYR174
C	HIS189
C	HOH1036
D	ASN42
D	CYS223
D	ASN224

site_id	AD6
Number of Residues	5
Details	binding site for residue PGE C 704

Chain	Residue
C	TRP48
C	GLN336
C	PHE340
C	HOH931
F	LEU507

site_id	AD7
Number of Residues	9
Details	binding site for residue GOL C 705

Chain	Residue
C	ASP635
C	LEU636
C	CA708
C	HOH844
C	HOH1072
C	HOH1091
E	LEU636
E	GLY637
E	TYR638

site_id	AD8
Number of Residues	1
Details	binding site for residue CL C 706

Chain	Residue
C	HOH1073

site_id	AD9
Number of Residues	6
Details	binding site for residue CA C 707

Chain	Residue
C	GLU382
C	HOH980
C	HOH1066
C	HOH1078
C	HOH1095
C	HOH1122

site_id	AE1
Number of Residues	6
Details	binding site for residue CA C 708

Chain	Residue
C	ASP635
C	GOL705
C	HOH844
C	HOH1072
C	HOH1091
C	HOH1101

site_id	AE2
Number of Residues	33
Details	binding site for residue FAS D 701

Chain	Residue
D	GLY13
D	GLY15
D	SER16
D	SER17
D	GLU38
D	ALA39
D	ALA87
D	VAL89
D	GLY92
D	GLY93
D	ASN97
D	PHE98
D	MET99
D	MET100
D	THR225
D	LYS226
D	VAL227
D	CYS268
D	GLY269
D	SER272
D	TRP566
D	HIS567
D	ASP605
D	LEU606
D	ASN616
D	THR617
D	TYR618
D	HOH803
D	HOH812
D	HOH825
D	HOH836
D	HOH851
D	HOH855

site_id	AE3
Number of Residues	12
Details	binding site for residue P6G D 702

Chain	Residue
D	LYS407
D	THR478
D	SER479
D	GLU494
D	TRP518
H	LYS407
H	THR478
H	SER479
H	LEU483
H	GLU494
H	TRP518
H	THR519

site_id	AE4
Number of Residues	4
Details	binding site for residue PGE D 703

Chain	Residue
D	TRP48
D	GLN336
D	PHE340
H	LEU507

site_id	AE5
Number of Residues	4
Details	binding site for residue CA D 704

Chain	Residue
D	GLU382
D	HOH807
D	HOH906
D	HOH962

site_id	AE6
Number of Residues	36
Details	binding site for residue FAS E 701

Chain	Residue
E	GLY13
E	GLY15
E	SER16
E	SER17
E	GLU38
E	ALA39
E	ALA87
E	VAL89
E	GLY92
E	GLY93
E	ILE96
E	ASN97
E	PHE98
E	MET99
E	MET100
E	THR225
E	LYS226
E	VAL227
E	SER267
E	CYS268
E	GLY269
E	SER272
E	TRP566
E	ASP605
E	LEU606
E	ASN616
E	THR617
E	TYR618
E	HOH834
E	HOH868
E	HOH927
E	HOH935
E	HOH937
E	HOH973
E	HOH985
E	HOH1037

site_id	AE7
Number of Residues	10
Details	binding site for residue P6G E 702

Chain	Residue
E	ASN42
E	LEU44
E	LEU221
E	CYS223
E	ASN224
E	HOH819
F	GLN161
F	LEU164
F	GLU168
F	TYR174

site_id	AE8
Number of Residues	6
Details	binding site for residue PGE E 703

Chain	Residue
A	PRO506
A	LEU507
A	LEU509
E	TRP48
E	GLN336
E	HOH891

site_id	AE9
Number of Residues	2
Details	binding site for residue CA E 704

Chain	Residue
E	GLU382
E	HOH951

site_id	AF1
Number of Residues	36
Details	binding site for residue FAS F 701

Chain	Residue
F	GLY13
F	GLY15
F	SER16
F	SER17
F	GLU38
F	ALA39
F	MET59
F	ALA87
F	VAL89
F	GLY92
F	GLY93
F	ILE96
F	ASN97
F	PHE98
F	MET99
F	MET100
F	THR225
F	LYS226
F	VAL227
F	CYS268
F	GLY269
F	SER272
F	TRP566
F	HIS567
F	ASP605
F	LEU606
F	ASN616
F	THR617
F	TYR618
F	HOH842
F	HOH869
F	HOH877
F	HOH895
F	HOH938
F	HOH941
F	HOH1014

site_id	AF2
Number of Residues	11
Details	binding site for residue P6G F 702

Chain	Residue
C	LYS407
C	THR478
C	SER479
C	GLU494
C	TRP518
C	THR519
F	LYS407
F	THR478
F	SER479
F	GLU494
F	TRP518

site_id	AF3
Number of Residues	13
Details	binding site for residue P6G F 703

Chain	Residue
F	ASN42
F	LEU44
F	LEU221
F	CYS223
F	ASN224
F	HOH993
H	CYS160
H	GLN161
H	LEU164
H	ARG165
H	GLU168
H	TYR174
H	HIS189

site_id	AF4
Number of Residues	9
Details	binding site for residue PO4 F 704

Chain	Residue
E	ASP341
E	HOH808
F	ASP341
F	HOH801
F	HOH803
F	HOH871
F	HOH888
G	ASP341
H	ASP341

site_id	AF5
Number of Residues	3
Details	binding site for residue GOL F 705

Chain	Residue
F	TRP48
F	GLN336
F	HOH809

site_id	AF6
Number of Residues	4
Details	binding site for residue CA F 706

Chain	Residue
F	GLU382
F	HOH979
F	HOH1028
F	HOH1089

site_id	AF7
Number of Residues	39
Details	binding site for residue FAS G 701

Chain	Residue
G	GLY13
G	GLY15
G	SER16
G	SER17
G	GLU38
G	ALA39
G	MET59
G	ALA87
G	VAL89
G	GLY92
G	GLY93
G	ILE96
G	ASN97
G	PHE98
G	MET99
G	MET100
G	THR225
G	LYS226
G	VAL227
G	SER267
G	CYS268
G	SER272
G	TRP566
G	HIS567
G	ASP605
G	LEU606
G	ASN616
G	THR617
G	TYR618
G	HOH840
G	HOH849
G	HOH871
G	HOH872
G	HOH874
G	HOH897
G	HOH899
G	HOH904
G	HOH909
G	HOH936

site_id	AF8
Number of Residues	8
Details	binding site for residue PGE G 702

Chain	Residue
E	GLN161
E	TYR174
E	HIS189
G	ASN42
G	LEU44
G	LEU221
G	CYS223
G	HOH938

site_id	AF9
Number of Residues	6
Details	binding site for residue PGE G 703

Chain	Residue
G	GLN161
G	TYR174
G	HOH908
H	ASN42
H	LEU44
H	CYS223

site_id	AG1
Number of Residues	3
Details	binding site for residue CA G 704

Chain	Residue
G	GLU382
G	HOH953
G	HOH988

site_id	AG2
Number of Residues	34
Details	binding site for residue FAS H 701

Chain	Residue
H	GLY13
H	GLY15
H	SER16
H	SER17
H	GLU38
H	ALA39
H	ALA87
H	VAL89
H	GLY92
H	GLY93
H	ASN97
H	PHE98
H	MET99
H	MET100
H	TRP193
H	LYS226
H	VAL227
H	SER267
H	CYS268
H	GLY269
H	SER272
H	TRP566
H	ASP605
H	LEU606
H	ASN616
H	THR617
H	TYR618
H	ALA621
H	HOH801
H	HOH813
H	HOH827
H	HOH830
H	HOH836
H	HOH845

site_id	AG3
Number of Residues	2
Details	binding site for residue PGE H 702

Chain	Residue
H	TRP48
H	GLN336

Functional Information from PROSITE/UniProt

site_id	PS00623
Number of Residues	24
Details	GMC_OXRED_1 GMC oxidoreductases signature 1. ANvLGGGSsINfmmYtrGsasdyD

Chain	Residue	Details
A	ALA87-ASP110

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	16
Details	Motif: {"description":"Microbody targeting signal","evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	8
Details	Active site: {"description":"Proton acceptor","evidences":[{"source":"UniProtKB","id":"E4QP00","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	240
Details	Binding site: {"evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)