5HM8
2.85 Angstrom Crystal Structure of S-adenosylhomocysteinase from Cryptosporidium parvum in Complex with Adenosine and NAD.
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000166 | molecular_function | nucleotide binding |
| A | 0004013 | molecular_function | adenosylhomocysteinase activity |
| A | 0005829 | cellular_component | cytosol |
| A | 0006730 | biological_process | one-carbon metabolic process |
| A | 0016787 | molecular_function | hydrolase activity |
| A | 0033353 | biological_process | S-adenosylmethionine cycle |
| B | 0000166 | molecular_function | nucleotide binding |
| B | 0004013 | molecular_function | adenosylhomocysteinase activity |
| B | 0005829 | cellular_component | cytosol |
| B | 0006730 | biological_process | one-carbon metabolic process |
| B | 0016787 | molecular_function | hydrolase activity |
| B | 0033353 | biological_process | S-adenosylmethionine cycle |
| C | 0000166 | molecular_function | nucleotide binding |
| C | 0004013 | molecular_function | adenosylhomocysteinase activity |
| C | 0005829 | cellular_component | cytosol |
| C | 0006730 | biological_process | one-carbon metabolic process |
| C | 0016787 | molecular_function | hydrolase activity |
| C | 0033353 | biological_process | S-adenosylmethionine cycle |
| D | 0000166 | molecular_function | nucleotide binding |
| D | 0004013 | molecular_function | adenosylhomocysteinase activity |
| D | 0005829 | cellular_component | cytosol |
| D | 0006730 | biological_process | one-carbon metabolic process |
| D | 0016787 | molecular_function | hydrolase activity |
| D | 0033353 | biological_process | S-adenosylmethionine cycle |
| E | 0000166 | molecular_function | nucleotide binding |
| E | 0004013 | molecular_function | adenosylhomocysteinase activity |
| E | 0005829 | cellular_component | cytosol |
| E | 0006730 | biological_process | one-carbon metabolic process |
| E | 0016787 | molecular_function | hydrolase activity |
| E | 0033353 | biological_process | S-adenosylmethionine cycle |
| F | 0000166 | molecular_function | nucleotide binding |
| F | 0004013 | molecular_function | adenosylhomocysteinase activity |
| F | 0005829 | cellular_component | cytosol |
| F | 0006730 | biological_process | one-carbon metabolic process |
| F | 0016787 | molecular_function | hydrolase activity |
| F | 0033353 | biological_process | S-adenosylmethionine cycle |
| G | 0000166 | molecular_function | nucleotide binding |
| G | 0004013 | molecular_function | adenosylhomocysteinase activity |
| G | 0005829 | cellular_component | cytosol |
| G | 0006730 | biological_process | one-carbon metabolic process |
| G | 0016787 | molecular_function | hydrolase activity |
| G | 0033353 | biological_process | S-adenosylmethionine cycle |
| H | 0000166 | molecular_function | nucleotide binding |
| H | 0004013 | molecular_function | adenosylhomocysteinase activity |
| H | 0005829 | cellular_component | cytosol |
| H | 0006730 | biological_process | one-carbon metabolic process |
| H | 0016787 | molecular_function | hydrolase activity |
| H | 0033353 | biological_process | S-adenosylmethionine cycle |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 15 |
| Details | binding site for residue ADN A 501 |
| Chain | Residue |
| A | HIS53 |
| A | HIS356 |
| A | THR408 |
| A | HIS410 |
| A | MET415 |
| A | PHE419 |
| A | NAD502 |
| A | THR55 |
| A | GLU57 |
| A | THR58 |
| A | ASP137 |
| A | GLU211 |
| A | THR212 |
| A | LYS241 |
| A | ASP245 |
| site_id | AC2 |
| Number of Residues | 25 |
| Details | binding site for residue NAD A 502 |
| Chain | Residue |
| A | THR212 |
| A | THR213 |
| A | THR214 |
| A | ASN246 |
| A | GLY275 |
| A | GLY277 |
| A | GLU278 |
| A | VAL279 |
| A | THR297 |
| A | GLU298 |
| A | ILE299 |
| A | THR331 |
| A | GLY332 |
| A | ASN333 |
| A | ILE354 |
| A | GLY355 |
| A | HIS356 |
| A | LEU401 |
| A | ASN403 |
| A | HIS410 |
| A | ADN501 |
| A | HOH640 |
| B | GLN476 |
| B | LYS489 |
| B | TYR493 |
| site_id | AC3 |
| Number of Residues | 1 |
| Details | binding site for residue CL A 503 |
| Chain | Residue |
| A | ARG6 |
| site_id | AC4 |
| Number of Residues | 2 |
| Details | binding site for residue CL A 504 |
| Chain | Residue |
| A | ARG35 |
| A | HOH676 |
| site_id | AC5 |
| Number of Residues | 2 |
| Details | binding site for residue CL A 505 |
| Chain | Residue |
| A | LYS270 |
| A | ARG293 |
| site_id | AC6 |
| Number of Residues | 4 |
| Details | binding site for residue SO4 A 506 |
| Chain | Residue |
| A | LYS2 |
| A | MET3 |
| A | GLU4 |
| A | ARG6 |
| site_id | AC7 |
| Number of Residues | 15 |
| Details | binding site for residue ADN B 501 |
| Chain | Residue |
| B | HIS53 |
| B | THR55 |
| B | GLU57 |
| B | THR58 |
| B | ASP137 |
| B | GLU211 |
| B | THR212 |
| B | LYS241 |
| B | ASP245 |
| B | HIS356 |
| B | LEU401 |
| B | THR408 |
| B | HIS410 |
| B | MET415 |
| B | NAD502 |
| site_id | AC8 |
| Number of Residues | 25 |
| Details | binding site for residue NAD B 502 |
| Chain | Residue |
| A | GLN476 |
| A | LYS489 |
| A | TYR493 |
| B | THR212 |
| B | THR213 |
| B | THR214 |
| B | ASN246 |
| B | GLY275 |
| B | GLY277 |
| B | GLU278 |
| B | VAL279 |
| B | THR297 |
| B | GLU298 |
| B | ILE299 |
| B | THR331 |
| B | GLY332 |
| B | ASN333 |
| B | ILE354 |
| B | GLY355 |
| B | HIS356 |
| B | LEU401 |
| B | ASN403 |
| B | HIS410 |
| B | ADN501 |
| B | HOH626 |
| site_id | AC9 |
| Number of Residues | 2 |
| Details | binding site for residue CL B 503 |
| Chain | Residue |
| B | LYS270 |
| B | ARG293 |
| site_id | AD1 |
| Number of Residues | 1 |
| Details | binding site for residue CL B 505 |
| Chain | Residue |
| B | ARG343 |
| site_id | AD2 |
| Number of Residues | 4 |
| Details | binding site for residue SO4 B 506 |
| Chain | Residue |
| B | LYS2 |
| B | MET3 |
| B | GLU4 |
| B | ARG6 |
| site_id | AD3 |
| Number of Residues | 15 |
| Details | binding site for residue ADN C 501 |
| Chain | Residue |
| C | THR58 |
| C | ASP137 |
| C | GLU211 |
| C | THR212 |
| C | LYS241 |
| C | ASP245 |
| C | HIS356 |
| C | LEU401 |
| C | THR408 |
| C | HIS410 |
| C | MET415 |
| C | NAD502 |
| C | HIS53 |
| C | THR55 |
| C | GLU57 |
| site_id | AD4 |
| Number of Residues | 22 |
| Details | binding site for residue NAD C 502 |
| Chain | Residue |
| C | THR212 |
| C | THR213 |
| C | THR214 |
| C | ASN246 |
| C | GLY277 |
| C | GLU278 |
| C | VAL279 |
| C | THR297 |
| C | GLU298 |
| C | ILE299 |
| C | THR331 |
| C | GLY332 |
| C | ASN333 |
| C | ILE354 |
| C | GLY355 |
| C | HIS356 |
| C | LEU401 |
| C | ASN403 |
| C | HIS410 |
| C | ADN501 |
| D | LYS489 |
| D | TYR493 |
| site_id | AD5 |
| Number of Residues | 3 |
| Details | binding site for residue CL C 503 |
| Chain | Residue |
| A | HOH674 |
| C | LYS270 |
| C | ARG293 |
| site_id | AD6 |
| Number of Residues | 1 |
| Details | binding site for residue CL C 504 |
| Chain | Residue |
| C | GLU491 |
| site_id | AD7 |
| Number of Residues | 4 |
| Details | binding site for residue SO4 C 505 |
| Chain | Residue |
| C | LYS2 |
| C | MET3 |
| C | GLU4 |
| C | ARG6 |
| site_id | AD8 |
| Number of Residues | 15 |
| Details | binding site for residue ADN D 501 |
| Chain | Residue |
| D | HIS53 |
| D | THR55 |
| D | GLU57 |
| D | THR58 |
| D | ASP137 |
| D | GLU211 |
| D | THR212 |
| D | LYS241 |
| D | ASP245 |
| D | HIS356 |
| D | LEU401 |
| D | THR408 |
| D | HIS410 |
| D | MET415 |
| D | NAD502 |
| site_id | AD9 |
| Number of Residues | 26 |
| Details | binding site for residue NAD D 502 |
| Chain | Residue |
| C | GLN476 |
| C | LYS489 |
| C | TYR493 |
| D | THR212 |
| D | THR213 |
| D | THR214 |
| D | ASN246 |
| D | GLY275 |
| D | GLY277 |
| D | GLU278 |
| D | VAL279 |
| D | THR297 |
| D | GLU298 |
| D | ILE299 |
| D | THR331 |
| D | GLY332 |
| D | ASN333 |
| D | ILE354 |
| D | GLY355 |
| D | HIS356 |
| D | LEU401 |
| D | ASN403 |
| D | HIS410 |
| D | ADN501 |
| D | HOH631 |
| D | HOH654 |
| site_id | AE1 |
| Number of Residues | 2 |
| Details | binding site for residue CL D 503 |
| Chain | Residue |
| D | GLU154 |
| D | TYR158 |
| site_id | AE2 |
| Number of Residues | 2 |
| Details | binding site for residue CL D 504 |
| Chain | Residue |
| D | LYS270 |
| D | ARG293 |
| site_id | AE3 |
| Number of Residues | 1 |
| Details | binding site for residue CL D 505 |
| Chain | Residue |
| D | ARG35 |
| site_id | AE4 |
| Number of Residues | 15 |
| Details | binding site for residue ADN E 501 |
| Chain | Residue |
| E | HIS53 |
| E | THR55 |
| E | GLU57 |
| E | THR58 |
| E | ASP137 |
| E | GLU211 |
| E | THR212 |
| E | LYS241 |
| E | ASP245 |
| E | HIS356 |
| E | LEU401 |
| E | THR408 |
| E | HIS410 |
| E | MET415 |
| E | NAD502 |
| site_id | AE5 |
| Number of Residues | 23 |
| Details | binding site for residue NAD E 502 |
| Chain | Residue |
| E | THR212 |
| E | THR213 |
| E | THR214 |
| E | ASN246 |
| E | GLY275 |
| E | GLY277 |
| E | GLU278 |
| E | VAL279 |
| E | GLU298 |
| E | ILE299 |
| E | THR331 |
| E | GLY332 |
| E | ASN333 |
| E | ILE354 |
| E | GLY355 |
| E | HIS356 |
| E | LEU401 |
| E | ASN403 |
| E | HIS410 |
| E | ADN501 |
| E | HOH634 |
| F | LYS489 |
| F | TYR493 |
| site_id | AE6 |
| Number of Residues | 1 |
| Details | binding site for residue CL E 503 |
| Chain | Residue |
| E | ARG343 |
| site_id | AE7 |
| Number of Residues | 4 |
| Details | binding site for residue SO4 E 504 |
| Chain | Residue |
| E | LYS2 |
| E | MET3 |
| E | GLU4 |
| E | ARG6 |
| site_id | AE8 |
| Number of Residues | 15 |
| Details | binding site for residue ADN F 501 |
| Chain | Residue |
| F | HIS53 |
| F | THR55 |
| F | GLU57 |
| F | THR58 |
| F | ASP137 |
| F | GLU211 |
| F | THR212 |
| F | LYS241 |
| F | ASP245 |
| F | HIS356 |
| F | LEU401 |
| F | THR408 |
| F | HIS410 |
| F | MET415 |
| F | NAD502 |
| site_id | AE9 |
| Number of Residues | 27 |
| Details | binding site for residue NAD F 502 |
| Chain | Residue |
| E | GLN476 |
| E | LYS489 |
| E | TYR493 |
| F | THR212 |
| F | THR213 |
| F | THR214 |
| F | ASN246 |
| F | GLY275 |
| F | GLY277 |
| F | GLU278 |
| F | VAL279 |
| F | THR297 |
| F | GLU298 |
| F | ILE299 |
| F | THR331 |
| F | GLY332 |
| F | ASN333 |
| F | ILE354 |
| F | GLY355 |
| F | HIS356 |
| F | LEU401 |
| F | ASN403 |
| F | HIS410 |
| F | ADN501 |
| F | HOH624 |
| F | HOH628 |
| F | HOH641 |
| site_id | AF1 |
| Number of Residues | 2 |
| Details | binding site for residue CL F 503 |
| Chain | Residue |
| F | LYS270 |
| F | ARG293 |
| site_id | AF2 |
| Number of Residues | 15 |
| Details | binding site for residue ADN G 501 |
| Chain | Residue |
| G | HIS53 |
| G | THR55 |
| G | GLU57 |
| G | THR58 |
| G | ASP137 |
| G | GLU211 |
| G | THR212 |
| G | LYS241 |
| G | ASP245 |
| G | HIS356 |
| G | THR408 |
| G | HIS410 |
| G | MET415 |
| G | PHE419 |
| G | NAD502 |
| site_id | AF3 |
| Number of Residues | 23 |
| Details | binding site for residue NAD G 502 |
| Chain | Residue |
| G | THR212 |
| G | THR213 |
| G | THR214 |
| G | ASN246 |
| G | GLY277 |
| G | GLU278 |
| G | VAL279 |
| G | THR297 |
| G | GLU298 |
| G | ILE299 |
| G | THR331 |
| G | GLY332 |
| G | ASN333 |
| G | ILE354 |
| G | GLY355 |
| G | HIS356 |
| G | LEU401 |
| G | ASN403 |
| G | HIS410 |
| G | ADN501 |
| H | GLN476 |
| H | LYS489 |
| H | TYR493 |
| site_id | AF4 |
| Number of Residues | 1 |
| Details | binding site for residue CL G 503 |
| Chain | Residue |
| G | ARG35 |
| site_id | AF5 |
| Number of Residues | 1 |
| Details | binding site for residue CL G 504 |
| Chain | Residue |
| G | ARG6 |
| site_id | AF6 |
| Number of Residues | 3 |
| Details | binding site for residue CL G 505 |
| Chain | Residue |
| E | HOH673 |
| G | LYS270 |
| G | ARG293 |
| site_id | AF7 |
| Number of Residues | 4 |
| Details | binding site for residue SO4 G 506 |
| Chain | Residue |
| G | LYS2 |
| G | MET3 |
| G | GLU4 |
| G | ARG6 |
| site_id | AF8 |
| Number of Residues | 15 |
| Details | binding site for residue ADN H 501 |
| Chain | Residue |
| H | HIS53 |
| H | THR55 |
| H | GLU57 |
| H | THR58 |
| H | ASP137 |
| H | GLU211 |
| H | THR212 |
| H | LYS241 |
| H | ASP245 |
| H | HIS356 |
| H | LEU401 |
| H | THR408 |
| H | HIS410 |
| H | MET415 |
| H | NAD502 |
| site_id | AF9 |
| Number of Residues | 28 |
| Details | binding site for residue NAD H 502 |
| Chain | Residue |
| G | GLN476 |
| G | LYS489 |
| G | TYR493 |
| H | THR212 |
| H | THR213 |
| H | THR214 |
| H | ASN246 |
| H | GLY275 |
| H | GLY277 |
| H | GLU278 |
| H | VAL279 |
| H | THR297 |
| H | GLU298 |
| H | ILE299 |
| H | CYS303 |
| H | THR331 |
| H | GLY332 |
| H | ASN333 |
| H | ILE354 |
| H | GLY355 |
| H | HIS356 |
| H | LEU401 |
| H | ASN403 |
| H | HIS410 |
| H | ADN501 |
| H | HOH615 |
| H | HOH629 |
| H | HOH633 |
| site_id | AG1 |
| Number of Residues | 2 |
| Details | binding site for residue CL H 503 |
| Chain | Residue |
| H | LYS270 |
| H | ARG293 |
Functional Information from PROSITE/UniProt
| site_id | PS00738 |
| Number of Residues | 15 |
| Details | ADOHCYASE_1 S-adenosyl-L-homocysteine hydrolase signature 1. SCNiYSTQDhAAAAL |
| Chain | Residue | Details |
| A | SER76-LEU90 |
| site_id | PS00739 |
| Number of Residues | 17 |
| Details | ADOHCYASE_2 S-adenosyl-L-homocysteine hydrolase signature 2. GKkivVlGYGeVGKGc.A |
| Chain | Residue | Details |
| A | GLY268-ALA284 |
