Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

5HAT

Structure function studies of R. palustris RubisCO (S59F/M331A mutant; CABP-bound)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000287	molecular_function	magnesium ion binding
A	0004497	molecular_function	monooxygenase activity
A	0015977	biological_process	carbon fixation
A	0015979	biological_process	photosynthesis
A	0016491	molecular_function	oxidoreductase activity
A	0016829	molecular_function	lyase activity
A	0016984	molecular_function	ribulose-bisphosphate carboxylase activity
A	0019253	biological_process	reductive pentose-phosphate cycle
A	0046872	molecular_function	metal ion binding
B	0000287	molecular_function	magnesium ion binding
B	0004497	molecular_function	monooxygenase activity
B	0015977	biological_process	carbon fixation
B	0015979	biological_process	photosynthesis
B	0016491	molecular_function	oxidoreductase activity
B	0016829	molecular_function	lyase activity
B	0016984	molecular_function	ribulose-bisphosphate carboxylase activity
B	0019253	biological_process	reductive pentose-phosphate cycle
B	0046872	molecular_function	metal ion binding
C	0000287	molecular_function	magnesium ion binding
C	0004497	molecular_function	monooxygenase activity
C	0015977	biological_process	carbon fixation
C	0015979	biological_process	photosynthesis
C	0016491	molecular_function	oxidoreductase activity
C	0016829	molecular_function	lyase activity
C	0016984	molecular_function	ribulose-bisphosphate carboxylase activity
C	0019253	biological_process	reductive pentose-phosphate cycle
C	0046872	molecular_function	metal ion binding
D	0000287	molecular_function	magnesium ion binding
D	0004497	molecular_function	monooxygenase activity
D	0015977	biological_process	carbon fixation
D	0015979	biological_process	photosynthesis
D	0016491	molecular_function	oxidoreductase activity
D	0016829	molecular_function	lyase activity
D	0016984	molecular_function	ribulose-bisphosphate carboxylase activity
D	0019253	biological_process	reductive pentose-phosphate cycle
D	0046872	molecular_function	metal ion binding
E	0000287	molecular_function	magnesium ion binding
E	0004497	molecular_function	monooxygenase activity
E	0015977	biological_process	carbon fixation
E	0015979	biological_process	photosynthesis
E	0016491	molecular_function	oxidoreductase activity
E	0016829	molecular_function	lyase activity
E	0016984	molecular_function	ribulose-bisphosphate carboxylase activity
E	0019253	biological_process	reductive pentose-phosphate cycle
E	0046872	molecular_function	metal ion binding
F	0000287	molecular_function	magnesium ion binding
F	0004497	molecular_function	monooxygenase activity
F	0015977	biological_process	carbon fixation
F	0015979	biological_process	photosynthesis
F	0016491	molecular_function	oxidoreductase activity
F	0016829	molecular_function	lyase activity
F	0016984	molecular_function	ribulose-bisphosphate carboxylase activity
F	0019253	biological_process	reductive pentose-phosphate cycle
F	0046872	molecular_function	metal ion binding
G	0000287	molecular_function	magnesium ion binding
G	0004497	molecular_function	monooxygenase activity
G	0015977	biological_process	carbon fixation
G	0015979	biological_process	photosynthesis
G	0016491	molecular_function	oxidoreductase activity
G	0016829	molecular_function	lyase activity
G	0016984	molecular_function	ribulose-bisphosphate carboxylase activity
G	0019253	biological_process	reductive pentose-phosphate cycle
G	0046872	molecular_function	metal ion binding
H	0000287	molecular_function	magnesium ion binding
H	0004497	molecular_function	monooxygenase activity
H	0015977	biological_process	carbon fixation
H	0015979	biological_process	photosynthesis
H	0016491	molecular_function	oxidoreductase activity
H	0016829	molecular_function	lyase activity
H	0016984	molecular_function	ribulose-bisphosphate carboxylase activity
H	0019253	biological_process	reductive pentose-phosphate cycle
H	0046872	molecular_function	metal ion binding
I	0000287	molecular_function	magnesium ion binding
I	0004497	molecular_function	monooxygenase activity
I	0015977	biological_process	carbon fixation
I	0015979	biological_process	photosynthesis
I	0016491	molecular_function	oxidoreductase activity
I	0016829	molecular_function	lyase activity
I	0016984	molecular_function	ribulose-bisphosphate carboxylase activity
I	0019253	biological_process	reductive pentose-phosphate cycle
I	0046872	molecular_function	metal ion binding
J	0000287	molecular_function	magnesium ion binding
J	0004497	molecular_function	monooxygenase activity
J	0015977	biological_process	carbon fixation
J	0015979	biological_process	photosynthesis
J	0016491	molecular_function	oxidoreductase activity
J	0016829	molecular_function	lyase activity
J	0016984	molecular_function	ribulose-bisphosphate carboxylase activity
J	0019253	biological_process	reductive pentose-phosphate cycle
J	0046872	molecular_function	metal ion binding
K	0000287	molecular_function	magnesium ion binding
K	0004497	molecular_function	monooxygenase activity
K	0015977	biological_process	carbon fixation
K	0015979	biological_process	photosynthesis
K	0016491	molecular_function	oxidoreductase activity
K	0016829	molecular_function	lyase activity
K	0016984	molecular_function	ribulose-bisphosphate carboxylase activity
K	0019253	biological_process	reductive pentose-phosphate cycle
K	0046872	molecular_function	metal ion binding
L	0000287	molecular_function	magnesium ion binding
L	0004497	molecular_function	monooxygenase activity
L	0015977	biological_process	carbon fixation
L	0015979	biological_process	photosynthesis
L	0016491	molecular_function	oxidoreductase activity
L	0016829	molecular_function	lyase activity
L	0016984	molecular_function	ribulose-bisphosphate carboxylase activity
L	0019253	biological_process	reductive pentose-phosphate cycle
L	0046872	molecular_function	metal ion binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	28
Details	binding site for residue CAP A 500

Chain	Residue
A	ILE165
A	LYS330
A	SER369
A	GLY370
A	GLY371
A	GLY394
A	GLY395
A	MG501
A	HOH616
A	HOH629
A	HOH632
A	LYS167
A	HOH633
A	HOH642
A	HOH671
A	HOH755
B	GLU49
B	THR54
B	ASN112
B	HOH638
B	HOH685
A	LYS169
A	KCX192
A	ASP194
A	GLU195
A	HIS288
A	ARG289
A	HIS322

site_id	AC2
Number of Residues	4
Details	binding site for residue MG A 501

Chain	Residue
A	KCX192
A	ASP194
A	GLU195
A	CAP500

site_id	AC3
Number of Residues	27
Details	binding site for residue CAP B 500

Chain	Residue
A	GLU49
A	THR54
A	ASN112
A	HOH621
B	ILE165
B	LYS167
B	LYS169
B	KCX192
B	ASP194
B	GLU195
B	HIS288
B	ARG289
B	HIS322
B	LYS330
B	SER369
B	GLY370
B	GLY371
B	GLY394
B	GLY395
B	MG501
B	HOH631
B	HOH643
B	HOH644
B	HOH645
B	HOH648
B	HOH653
B	HOH673

site_id	AC4
Number of Residues	4
Details	binding site for residue MG B 501

Chain	Residue
B	KCX192
B	ASP194
B	GLU195
B	CAP500

site_id	AC5
Number of Residues	25
Details	binding site for residue CAP C 500

Chain	Residue
C	ILE165
C	LYS167
C	LYS169
C	KCX192
C	ASP194
C	GLU195
C	HIS288
C	ARG289
C	HIS322
C	LYS330
C	SER369
C	GLY370
C	GLY371
C	GLY394
C	GLY395
C	MG501
C	HOH616
C	HOH640
C	HOH658
C	HOH662
C	HOH682
D	GLU49
D	THR54
D	ASN112
D	HOH619

site_id	AC6
Number of Residues	4
Details	binding site for residue MG C 501

Chain	Residue
C	KCX192
C	ASP194
C	GLU195
C	CAP500

site_id	AC7
Number of Residues	28
Details	binding site for residue CAP D 500

Chain	Residue
D	LYS169
D	KCX192
D	ASP194
D	GLU195
D	HIS288
D	ARG289
D	HIS322
D	LYS330
D	SER369
D	GLY370
D	GLY371
D	GLY394
D	GLY395
D	MG501
D	HOH621
D	HOH637
D	HOH638
D	HOH660
D	HOH684
D	HOH687
D	HOH739
C	GLU49
C	THR54
C	ASN112
C	HOH603
C	HOH692
D	ILE165
D	LYS167

site_id	AC8
Number of Residues	4
Details	binding site for residue MG D 501

Chain	Residue
D	KCX192
D	ASP194
D	GLU195
D	CAP500

site_id	AC9
Number of Residues	27
Details	binding site for residue CAP E 500

Chain	Residue
E	ILE165
E	LYS167
E	LYS169
E	KCX192
E	ASP194
E	GLU195
E	HIS288
E	ARG289
E	HIS322
E	LYS330
E	SER369
E	GLY370
E	GLY371
E	GLY394
E	GLY395
E	MG501
E	HOH612
E	HOH614
E	HOH626
E	HOH664
E	HOH684
E	HOH698
E	HOH743
F	GLU49
F	THR54
F	ASN112
F	HOH617

site_id	AD1
Number of Residues	4
Details	binding site for residue MG E 501

Chain	Residue
E	KCX192
E	ASP194
E	GLU195
E	CAP500

site_id	AD2
Number of Residues	25
Details	binding site for residue CAP F 500

Chain	Residue
E	GLU49
E	THR54
E	ASN112
F	ILE165
F	LYS167
F	LYS169
F	KCX192
F	ASP194
F	GLU195
F	HIS288
F	ARG289
F	HIS322
F	LYS330
F	SER369
F	GLY370
F	GLY371
F	GLY394
F	GLY395
F	MG501
F	HOH627
F	HOH631
F	HOH664
F	HOH669
F	HOH689
F	HOH711

site_id	AD3
Number of Residues	4
Details	binding site for residue MG F 501

Chain	Residue
F	KCX192
F	ASP194
F	GLU195
F	CAP500

site_id	AD4
Number of Residues	27
Details	binding site for residue CAP G 500

Chain	Residue
G	ILE165
G	LYS167
G	LYS169
G	KCX192
G	ASP194
G	GLU195
G	HIS288
G	ARG289
G	HIS322
G	LYS330
G	SER369
G	GLY370
G	GLY371
G	GLY394
G	GLY395
G	MG501
G	HOH612
G	HOH624
G	HOH638
G	HOH675
G	HOH685
G	HOH693
G	HOH703
H	GLU49
H	THR54
H	ASN112
H	HOH677

site_id	AD5
Number of Residues	4
Details	binding site for residue MG G 501

Chain	Residue
G	KCX192
G	ASP194
G	GLU195
G	CAP500

site_id	AD6
Number of Residues	28
Details	binding site for residue CAP H 500

Chain	Residue
G	GLU49
G	THR54
G	ASN112
G	HOH642
G	HOH665
H	ILE165
H	LYS167
H	LYS169
H	KCX192
H	ASP194
H	GLU195
H	HIS288
H	ARG289
H	HIS322
H	LYS330
H	SER369
H	GLY370
H	GLY371
H	GLY394
H	GLY395
H	MG501
H	HOH605
H	HOH628
H	HOH643
H	HOH647
H	HOH666
H	HOH669
H	HOH723

site_id	AD7
Number of Residues	5
Details	binding site for residue MG H 501

Chain	Residue
H	LYS169
H	KCX192
H	ASP194
H	GLU195
H	CAP500

site_id	AD8
Number of Residues	29
Details	binding site for residue CAP I 500

Chain	Residue
I	ILE165
I	LYS167
I	LYS169
I	KCX192
I	ASP194
I	GLU195
I	HIS288
I	ARG289
I	HIS322
I	LYS330
I	SER369
I	GLY370
I	GLY371
I	GLY394
I	GLY395
I	MG501
I	HOH610
I	HOH616
I	HOH618
I	HOH640
I	HOH651
I	HOH680
I	HOH696
I	HOH757
J	GLU49
J	THR54
J	ASN112
J	HOH627
J	HOH646

site_id	AD9
Number of Residues	4
Details	binding site for residue MG I 501

Chain	Residue
I	KCX192
I	ASP194
I	GLU195
I	CAP500

site_id	AE1
Number of Residues	28
Details	binding site for residue CAP J 500

Chain	Residue
I	GLU49
I	THR54
I	ASN112
I	HOH622
I	HOH663
J	ILE165
J	LYS167
J	LYS169
J	KCX192
J	ASP194
J	GLU195
J	HIS288
J	ARG289
J	HIS322
J	LYS330
J	SER369
J	GLY370
J	GLY371
J	GLY394
J	GLY395
J	MG501
J	HOH633
J	HOH640
J	HOH647
J	HOH648
J	HOH674
J	HOH693
J	HOH703

site_id	AE2
Number of Residues	4
Details	binding site for residue MG J 501

Chain	Residue
J	KCX192
J	ASP194
J	GLU195
J	CAP500

site_id	AE3
Number of Residues	24
Details	binding site for residue CAP K 500

Chain	Residue
K	ILE165
K	LYS167
K	LYS169
K	KCX192
K	ASP194
K	GLU195
K	HIS288
K	ARG289
K	HIS322
K	LYS330
K	SER369
K	GLY370
K	GLY371
K	GLY394
K	GLY395
K	MG501
K	HOH632
K	HOH638
K	HOH640
K	HOH693
L	GLU49
L	THR54
L	ASN112
L	HOH682

site_id	AE4
Number of Residues	4
Details	binding site for residue MG K 501

Chain	Residue
K	KCX192
K	ASP194
K	GLU195
K	CAP500

site_id	AE5
Number of Residues	25
Details	binding site for residue CAP L 500

Chain	Residue
K	GLU49
K	THR54
K	ASN112
K	HOH652
K	HOH680
L	ILE165
L	LYS167
L	LYS169
L	KCX192
L	ASP194
L	GLU195
L	HIS288
L	ARG289
L	HIS322
L	LYS330
L	SER369
L	GLY370
L	GLY371
L	GLY394
L	GLY395
L	MG501
L	HOH630
L	HOH645
L	HOH648
L	HOH670

site_id	AE6
Number of Residues	4
Details	binding site for residue MG L 501

Chain	Residue
L	KCX192
L	ASP194
L	GLU195
L	CAP500

Functional Information from PROSITE/UniProt

site_id	PS00157
Number of Residues	9
Details	RUBISCO_LARGE Ribulose bisphosphate carboxylase large chain active site. GgDFiKnDE

Chain	Residue	Details
A	GLY187-GLU195

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	24
Details	Active site: {"description":"Proton acceptor","evidences":[{"source":"HAMAP-Rule","id":"MF_01339","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	12
Details	Binding site: {"description":"in homodimeric partner","evidences":[{"source":"HAMAP-Rule","id":"MF_01339","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	72
Details	Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_01339","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI4
Number of Residues	12
Details	Binding site: {"description":"via carbamate group","evidences":[{"source":"HAMAP-Rule","id":"MF_01339","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI5
Number of Residues	12
Details	Site: {"description":"Transition state stabilizer","evidences":[{"source":"HAMAP-Rule","id":"MF_01339","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI6
Number of Residues	12
Details	Modified residue: {"description":"N6-carboxylysine","evidences":[{"source":"HAMAP-Rule","id":"MF_01339","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)