5H8L
Crystal structure of Medicago truncatula N-carbamoylputrescine amidohydrolase (MtCPA) C158S mutant in complex with putrescine
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0006596 | biological_process | polyamine biosynthetic process |
| A | 0016787 | molecular_function | hydrolase activity |
| A | 0016811 | molecular_function | hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides |
| A | 0033388 | biological_process | putrescine biosynthetic process from arginine |
| A | 0050126 | molecular_function | N-carbamoylputrescine amidase activity |
| B | 0006596 | biological_process | polyamine biosynthetic process |
| B | 0016787 | molecular_function | hydrolase activity |
| B | 0016811 | molecular_function | hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides |
| B | 0033388 | biological_process | putrescine biosynthetic process from arginine |
| B | 0050126 | molecular_function | N-carbamoylputrescine amidase activity |
| C | 0006596 | biological_process | polyamine biosynthetic process |
| C | 0016787 | molecular_function | hydrolase activity |
| C | 0016811 | molecular_function | hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides |
| C | 0033388 | biological_process | putrescine biosynthetic process from arginine |
| C | 0050126 | molecular_function | N-carbamoylputrescine amidase activity |
| D | 0006596 | biological_process | polyamine biosynthetic process |
| D | 0016787 | molecular_function | hydrolase activity |
| D | 0016811 | molecular_function | hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides |
| D | 0033388 | biological_process | putrescine biosynthetic process from arginine |
| D | 0050126 | molecular_function | N-carbamoylputrescine amidase activity |
| E | 0006596 | biological_process | polyamine biosynthetic process |
| E | 0016787 | molecular_function | hydrolase activity |
| E | 0016811 | molecular_function | hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides |
| E | 0033388 | biological_process | putrescine biosynthetic process from arginine |
| E | 0050126 | molecular_function | N-carbamoylputrescine amidase activity |
| F | 0006596 | biological_process | polyamine biosynthetic process |
| F | 0016787 | molecular_function | hydrolase activity |
| F | 0016811 | molecular_function | hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides |
| F | 0033388 | biological_process | putrescine biosynthetic process from arginine |
| F | 0050126 | molecular_function | N-carbamoylputrescine amidase activity |
| G | 0006596 | biological_process | polyamine biosynthetic process |
| G | 0016787 | molecular_function | hydrolase activity |
| G | 0016811 | molecular_function | hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides |
| G | 0033388 | biological_process | putrescine biosynthetic process from arginine |
| G | 0050126 | molecular_function | N-carbamoylputrescine amidase activity |
| H | 0006596 | biological_process | polyamine biosynthetic process |
| H | 0016787 | molecular_function | hydrolase activity |
| H | 0016811 | molecular_function | hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides |
| H | 0033388 | biological_process | putrescine biosynthetic process from arginine |
| H | 0050126 | molecular_function | N-carbamoylputrescine amidase activity |
| I | 0006596 | biological_process | polyamine biosynthetic process |
| I | 0016787 | molecular_function | hydrolase activity |
| I | 0016811 | molecular_function | hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides |
| I | 0033388 | biological_process | putrescine biosynthetic process from arginine |
| I | 0050126 | molecular_function | N-carbamoylputrescine amidase activity |
| J | 0006596 | biological_process | polyamine biosynthetic process |
| J | 0016787 | molecular_function | hydrolase activity |
| J | 0016811 | molecular_function | hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides |
| J | 0033388 | biological_process | putrescine biosynthetic process from arginine |
| J | 0050126 | molecular_function | N-carbamoylputrescine amidase activity |
| K | 0006596 | biological_process | polyamine biosynthetic process |
| K | 0016787 | molecular_function | hydrolase activity |
| K | 0016811 | molecular_function | hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides |
| K | 0033388 | biological_process | putrescine biosynthetic process from arginine |
| K | 0050126 | molecular_function | N-carbamoylputrescine amidase activity |
| L | 0006596 | biological_process | polyamine biosynthetic process |
| L | 0016787 | molecular_function | hydrolase activity |
| L | 0016811 | molecular_function | hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides |
| L | 0033388 | biological_process | putrescine biosynthetic process from arginine |
| L | 0050126 | molecular_function | N-carbamoylputrescine amidase activity |
| M | 0006596 | biological_process | polyamine biosynthetic process |
| M | 0016787 | molecular_function | hydrolase activity |
| M | 0016811 | molecular_function | hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides |
| M | 0033388 | biological_process | putrescine biosynthetic process from arginine |
| M | 0050126 | molecular_function | N-carbamoylputrescine amidase activity |
| N | 0006596 | biological_process | polyamine biosynthetic process |
| N | 0016787 | molecular_function | hydrolase activity |
| N | 0016811 | molecular_function | hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides |
| N | 0033388 | biological_process | putrescine biosynthetic process from arginine |
| N | 0050126 | molecular_function | N-carbamoylputrescine amidase activity |
| O | 0006596 | biological_process | polyamine biosynthetic process |
| O | 0016787 | molecular_function | hydrolase activity |
| O | 0016811 | molecular_function | hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides |
| O | 0033388 | biological_process | putrescine biosynthetic process from arginine |
| O | 0050126 | molecular_function | N-carbamoylputrescine amidase activity |
| P | 0006596 | biological_process | polyamine biosynthetic process |
| P | 0016787 | molecular_function | hydrolase activity |
| P | 0016811 | molecular_function | hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides |
| P | 0033388 | biological_process | putrescine biosynthetic process from arginine |
| P | 0050126 | molecular_function | N-carbamoylputrescine amidase activity |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 2 |
| Details | binding site for residue GOL A 401 |
| Chain | Residue |
| A | THR75 |
| A | ARG78 |
| site_id | AC2 |
| Number of Residues | 2 |
| Details | binding site for residue GOL A 402 |
| Chain | Residue |
| A | GLN57 |
| A | GLY185 |
| site_id | AC3 |
| Number of Residues | 6 |
| Details | binding site for residue EDO A 403 |
| Chain | Residue |
| C | HOH615 |
| A | ARG283 |
| A | PRO285 |
| A | ASP286 |
| A | HOH526 |
| A | HOH558 |
| site_id | AC4 |
| Number of Residues | 9 |
| Details | binding site for residue PUT B 401 |
| Chain | Residue |
| A | TRP277 |
| B | TYR130 |
| B | SER158 |
| B | ALA183 |
| B | GLU187 |
| B | HOH506 |
| B | HOH523 |
| B | HOH560 |
| B | HOH593 |
| site_id | AC5 |
| Number of Residues | 6 |
| Details | binding site for residue GOL B 402 |
| Chain | Residue |
| A | MET273 |
| A | TRP277 |
| B | HIS198 |
| B | HOH517 |
| B | HOH535 |
| B | HOH731 |
| site_id | AC6 |
| Number of Residues | 6 |
| Details | binding site for residue GOL B 403 |
| Chain | Residue |
| B | GLU51 |
| B | PHE55 |
| B | ARG66 |
| B | HOH536 |
| B | HOH594 |
| B | HOH621 |
| site_id | AC7 |
| Number of Residues | 1 |
| Details | binding site for residue EDO B 404 |
| Chain | Residue |
| B | ILE249 |
| site_id | AC8 |
| Number of Residues | 4 |
| Details | binding site for residue EDO B 405 |
| Chain | Residue |
| A | HOH564 |
| B | GLN204 |
| B | GLU248 |
| B | ILE249 |
| site_id | AC9 |
| Number of Residues | 9 |
| Details | binding site for residue PUT C 401 |
| Chain | Residue |
| C | TYR130 |
| C | SER158 |
| C | ALA183 |
| C | GLU187 |
| C | HOH523 |
| C | HOH533 |
| C | HOH564 |
| C | HOH573 |
| D | TRP277 |
| site_id | AD1 |
| Number of Residues | 7 |
| Details | binding site for residue GOL C 402 |
| Chain | Residue |
| C | MET273 |
| C | TRP277 |
| C | HOH540 |
| C | HOH639 |
| C | HOH747 |
| D | SER195 |
| D | HIS198 |
| site_id | AD2 |
| Number of Residues | 4 |
| Details | binding site for residue GOL C 403 |
| Chain | Residue |
| C | THR75 |
| C | ARG78 |
| C | HOH542 |
| C | HOH602 |
| site_id | AD3 |
| Number of Residues | 6 |
| Details | binding site for residue EDO C 404 |
| Chain | Residue |
| C | PRO285 |
| C | ASP286 |
| C | HOH530 |
| C | HOH584 |
| C | HOH742 |
| F | HOH615 |
| site_id | AD4 |
| Number of Residues | 7 |
| Details | binding site for residue GOL D 401 |
| Chain | Residue |
| C | HIS198 |
| D | MET273 |
| D | TRP277 |
| D | HOH502 |
| D | HOH648 |
| D | HOH654 |
| D | HOH760 |
| site_id | AD5 |
| Number of Residues | 9 |
| Details | binding site for residue PUT D 402 |
| Chain | Residue |
| C | TRP277 |
| D | SER158 |
| D | TRP159 |
| D | ALA183 |
| D | GLU187 |
| D | HOH539 |
| D | HOH554 |
| D | HOH564 |
| D | HOH635 |
| site_id | AD6 |
| Number of Residues | 6 |
| Details | binding site for residue GOL D 403 |
| Chain | Residue |
| C | ARG201 |
| D | GLN204 |
| D | GLU248 |
| D | ILE249 |
| D | HOH639 |
| D | HOH686 |
| site_id | AD7 |
| Number of Residues | 6 |
| Details | binding site for residue EDO D 404 |
| Chain | Residue |
| A | HOH557 |
| C | VAL300 |
| D | PRO285 |
| D | ASP286 |
| D | HOH631 |
| D | HOH700 |
| site_id | AD8 |
| Number of Residues | 2 |
| Details | binding site for residue EDO D 405 |
| Chain | Residue |
| D | HOH590 |
| D | HOH703 |
| site_id | AD9 |
| Number of Residues | 4 |
| Details | binding site for residue PEG D 406 |
| Chain | Residue |
| D | ASP112 |
| D | THR114 |
| D | HOH574 |
| D | HOH592 |
| site_id | AE1 |
| Number of Residues | 8 |
| Details | binding site for residue PUT E 401 |
| Chain | Residue |
| E | HOH540 |
| E | HOH552 |
| E | HOH570 |
| F | TRP277 |
| F | HOH510 |
| E | SER158 |
| E | ALA183 |
| E | GLU187 |
| site_id | AE2 |
| Number of Residues | 6 |
| Details | binding site for residue GOL E 402 |
| Chain | Residue |
| E | HIS198 |
| E | HOH505 |
| E | HOH521 |
| E | HOH667 |
| F | MET273 |
| F | TRP277 |
| site_id | AE3 |
| Number of Residues | 5 |
| Details | binding site for residue GOL E 403 |
| Chain | Residue |
| E | MET273 |
| E | TRP277 |
| E | HOH510 |
| F | ILE193 |
| F | HIS198 |
| site_id | AE4 |
| Number of Residues | 5 |
| Details | binding site for residue GOL E 404 |
| Chain | Residue |
| E | SER23 |
| E | THR75 |
| E | ARG78 |
| E | HOH516 |
| E | HOH677 |
| site_id | AE5 |
| Number of Residues | 1 |
| Details | binding site for residue GOL E 405 |
| Chain | Residue |
| E | GLY113 |
| site_id | AE6 |
| Number of Residues | 7 |
| Details | binding site for residue PUT F 401 |
| Chain | Residue |
| F | TYR130 |
| F | SER158 |
| F | ALA183 |
| F | GLU187 |
| F | HOH502 |
| F | HOH539 |
| F | HOH566 |
| site_id | AE7 |
| Number of Residues | 4 |
| Details | binding site for residue GOL F 402 |
| Chain | Residue |
| F | SER23 |
| F | PRO74 |
| F | THR75 |
| F | ARG78 |
| site_id | AE8 |
| Number of Residues | 7 |
| Details | binding site for residue EDO F 403 |
| Chain | Residue |
| D | HOH671 |
| E | VAL300 |
| F | PRO285 |
| F | ASP286 |
| F | HOH553 |
| F | HOH615 |
| F | HOH650 |
| site_id | AE9 |
| Number of Residues | 3 |
| Details | binding site for residue EDO F 404 |
| Chain | Residue |
| F | THR24 |
| F | THR27 |
| F | HOH575 |
| site_id | AF1 |
| Number of Residues | 9 |
| Details | binding site for residue PUT G 401 |
| Chain | Residue |
| G | TYR130 |
| G | SER158 |
| G | ALA183 |
| G | GLU187 |
| G | HOH524 |
| G | HOH541 |
| G | HOH570 |
| G | HOH581 |
| H | TRP277 |
| site_id | AF2 |
| Number of Residues | 3 |
| Details | binding site for residue GOL G 402 |
| Chain | Residue |
| G | HIS198 |
| H | MET273 |
| H | TRP277 |
| site_id | AF3 |
| Number of Residues | 7 |
| Details | binding site for residue GOL G 403 |
| Chain | Residue |
| G | LYS256 |
| G | GLU257 |
| G | HOH517 |
| G | HOH590 |
| L | LEU32 |
| L | GLU258 |
| L | HOH630 |
| site_id | AF4 |
| Number of Residues | 4 |
| Details | binding site for residue GOL I 401 |
| Chain | Residue |
| I | SER23 |
| I | THR75 |
| I | ARG78 |
| I | HOH551 |
| site_id | AF5 |
| Number of Residues | 5 |
| Details | binding site for residue EDO I 402 |
| Chain | Residue |
| I | PRO285 |
| I | ASP286 |
| I | HOH549 |
| I | HOH620 |
| J | VAL300 |
| site_id | AF6 |
| Number of Residues | 2 |
| Details | binding site for residue EDO I 403 |
| Chain | Residue |
| I | GLY185 |
| I | HOH628 |
| site_id | AF7 |
| Number of Residues | 9 |
| Details | binding site for residue PUT J 401 |
| Chain | Residue |
| I | TRP277 |
| I | HOH535 |
| J | TYR130 |
| J | SER158 |
| J | ALA183 |
| J | GLU187 |
| J | HOH545 |
| J | HOH546 |
| J | HOH581 |
| site_id | AF8 |
| Number of Residues | 6 |
| Details | binding site for residue GOL J 402 |
| Chain | Residue |
| I | MET273 |
| I | TRP277 |
| J | HIS198 |
| J | HOH514 |
| J | HOH618 |
| J | HOH723 |
| site_id | AF9 |
| Number of Residues | 5 |
| Details | binding site for residue GOL J 403 |
| Chain | Residue |
| J | ALA171 |
| J | ARG274 |
| J | PHE280 |
| J | HOH509 |
| J | HOH638 |
| site_id | AG1 |
| Number of Residues | 6 |
| Details | binding site for residue GOL J 404 |
| Chain | Residue |
| J | GLU51 |
| J | PHE55 |
| J | ARG66 |
| J | HOH503 |
| J | HOH550 |
| J | HOH552 |
| site_id | AG2 |
| Number of Residues | 9 |
| Details | binding site for residue PUT K 401 |
| Chain | Residue |
| K | TYR130 |
| K | SER158 |
| K | ALA183 |
| K | GLU187 |
| K | HOH506 |
| K | HOH549 |
| K | HOH551 |
| K | HOH590 |
| L | TRP277 |
| site_id | AG3 |
| Number of Residues | 6 |
| Details | binding site for residue GOL K 402 |
| Chain | Residue |
| K | HIS198 |
| K | HOH543 |
| K | HOH580 |
| K | HOH726 |
| L | MET273 |
| L | TRP277 |
| site_id | AG4 |
| Number of Residues | 6 |
| Details | binding site for residue GOL K 403 |
| Chain | Residue |
| K | MET273 |
| K | TRP277 |
| K | HOH505 |
| K | HOH527 |
| L | SER195 |
| L | HIS198 |
| site_id | AG5 |
| Number of Residues | 6 |
| Details | binding site for residue GOL K 404 |
| Chain | Residue |
| K | SER23 |
| K | PRO74 |
| K | THR75 |
| K | ARG78 |
| K | HOH504 |
| K | HOH568 |
| site_id | AG6 |
| Number of Residues | 8 |
| Details | binding site for residue EDO K 405 |
| Chain | Residue |
| K | ARG283 |
| K | PRO285 |
| K | ASP286 |
| K | HOH548 |
| K | HOH555 |
| K | HOH632 |
| K | HOH685 |
| N | HOH548 |
| site_id | AG7 |
| Number of Residues | 3 |
| Details | binding site for residue PEG K 406 |
| Chain | Residue |
| K | LYS84 |
| K | ASP112 |
| K | GLY113 |
| site_id | AG8 |
| Number of Residues | 9 |
| Details | binding site for residue PUT L 401 |
| Chain | Residue |
| K | TRP277 |
| L | TYR130 |
| L | SER158 |
| L | TRP159 |
| L | ALA183 |
| L | GLU187 |
| L | HOH529 |
| L | HOH576 |
| L | HOH597 |
| site_id | AG9 |
| Number of Residues | 3 |
| Details | binding site for residue GOL L 402 |
| Chain | Residue |
| L | THR75 |
| L | ARG78 |
| L | HOH505 |
| site_id | AH1 |
| Number of Residues | 5 |
| Details | binding site for residue EDO L 403 |
| Chain | Residue |
| J | HOH556 |
| L | ARG283 |
| L | ASP286 |
| L | HOH527 |
| L | HOH558 |
| site_id | AH2 |
| Number of Residues | 5 |
| Details | binding site for residue EDO L 404 |
| Chain | Residue |
| B | ARG34 |
| L | ASP190 |
| L | HOH544 |
| L | HOH672 |
| L | HOH730 |
| site_id | AH3 |
| Number of Residues | 1 |
| Details | binding site for residue PEG L 405 |
| Chain | Residue |
| L | HOH615 |
| site_id | AH4 |
| Number of Residues | 9 |
| Details | binding site for residue PUT M 401 |
| Chain | Residue |
| M | TYR130 |
| M | SER158 |
| M | ALA183 |
| M | GLU187 |
| M | HOH520 |
| M | HOH531 |
| M | HOH551 |
| M | HOH554 |
| N | TRP277 |
| site_id | AH5 |
| Number of Residues | 7 |
| Details | binding site for residue GOL M 402 |
| Chain | Residue |
| M | SER23 |
| M | PRO74 |
| M | THR75 |
| M | ARG78 |
| M | HOH515 |
| M | HOH532 |
| M | HOH552 |
| site_id | AH6 |
| Number of Residues | 7 |
| Details | binding site for residue EDO M 403 |
| Chain | Residue |
| M | ARG283 |
| M | ASP286 |
| M | HOH548 |
| M | HOH636 |
| M | HOH644 |
| M | HOH651 |
| P | HOH509 |
| site_id | AH7 |
| Number of Residues | 7 |
| Details | binding site for residue GOL N 401 |
| Chain | Residue |
| M | SER195 |
| M | HIS198 |
| N | MET273 |
| N | TRP277 |
| N | HOH519 |
| N | HOH530 |
| N | HOH639 |
| site_id | AH8 |
| Number of Residues | 9 |
| Details | binding site for residue PUT N 402 |
| Chain | Residue |
| M | TRP277 |
| M | HOH559 |
| N | TYR130 |
| N | SER158 |
| N | ALA183 |
| N | GLU187 |
| N | HOH504 |
| N | HOH533 |
| N | HOH571 |
| site_id | AH9 |
| Number of Residues | 5 |
| Details | binding site for residue GOL N 403 |
| Chain | Residue |
| M | MET273 |
| M | TRP277 |
| N | HIS198 |
| N | HOH514 |
| N | HOH540 |
| site_id | AI1 |
| Number of Residues | 6 |
| Details | binding site for residue GOL N 404 |
| Chain | Residue |
| N | SER23 |
| N | THR75 |
| N | ARG78 |
| N | HOH536 |
| N | HOH554 |
| N | HOH575 |
| site_id | AI2 |
| Number of Residues | 4 |
| Details | binding site for residue GOL N 405 |
| Chain | Residue |
| N | LYS84 |
| N | ASP112 |
| N | GLY113 |
| N | HOH653 |
| site_id | AI3 |
| Number of Residues | 5 |
| Details | binding site for residue EDO N 406 |
| Chain | Residue |
| K | HOH529 |
| N | PRO285 |
| N | ASP286 |
| N | HOH525 |
| N | HOH613 |
| site_id | AI4 |
| Number of Residues | 9 |
| Details | binding site for residue PUT O 401 |
| Chain | Residue |
| O | TYR130 |
| O | SER158 |
| O | ALA183 |
| O | GLU187 |
| O | HOH523 |
| O | HOH550 |
| O | HOH569 |
| O | HOH598 |
| P | TRP277 |
| site_id | AI5 |
| Number of Residues | 7 |
| Details | binding site for residue GOL O 402 |
| Chain | Residue |
| O | SER195 |
| O | HIS198 |
| O | HOH555 |
| O | HOH570 |
| P | LEU211 |
| P | MET273 |
| P | TRP277 |
| site_id | AI6 |
| Number of Residues | 4 |
| Details | binding site for residue GOL P 401 |
| Chain | Residue |
| P | VAL22 |
| P | PRO74 |
| P | THR75 |
| P | ARG78 |
| site_id | AI7 |
| Number of Residues | 4 |
| Details | binding site for residue EDO P 402 |
| Chain | Residue |
| P | ASN100 |
| P | HIS102 |
| P | LYS133 |
| P | ASN137 |
| site_id | AI8 |
| Number of Residues | 6 |
| Details | binding site for residue EDO P 403 |
| Chain | Residue |
| M | HOH636 |
| N | PRO138 |
| P | PRO285 |
| P | ASP286 |
| P | HOH520 |
| P | HOH568 |
