5H8K
Crystal structure of Medicago truncatula N-carbamoylputrescine amidohydrolase (MtCPA) C158S mutant
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0006596 | biological_process | polyamine biosynthetic process |
| A | 0016787 | molecular_function | hydrolase activity |
| A | 0016811 | molecular_function | hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides |
| A | 0033388 | biological_process | putrescine biosynthetic process from arginine |
| A | 0050126 | molecular_function | N-carbamoylputrescine amidase activity |
| B | 0006596 | biological_process | polyamine biosynthetic process |
| B | 0016787 | molecular_function | hydrolase activity |
| B | 0016811 | molecular_function | hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides |
| B | 0033388 | biological_process | putrescine biosynthetic process from arginine |
| B | 0050126 | molecular_function | N-carbamoylputrescine amidase activity |
| C | 0006596 | biological_process | polyamine biosynthetic process |
| C | 0016787 | molecular_function | hydrolase activity |
| C | 0016811 | molecular_function | hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides |
| C | 0033388 | biological_process | putrescine biosynthetic process from arginine |
| C | 0050126 | molecular_function | N-carbamoylputrescine amidase activity |
| D | 0006596 | biological_process | polyamine biosynthetic process |
| D | 0016787 | molecular_function | hydrolase activity |
| D | 0016811 | molecular_function | hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides |
| D | 0033388 | biological_process | putrescine biosynthetic process from arginine |
| D | 0050126 | molecular_function | N-carbamoylputrescine amidase activity |
| E | 0006596 | biological_process | polyamine biosynthetic process |
| E | 0016787 | molecular_function | hydrolase activity |
| E | 0016811 | molecular_function | hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides |
| E | 0033388 | biological_process | putrescine biosynthetic process from arginine |
| E | 0050126 | molecular_function | N-carbamoylputrescine amidase activity |
| F | 0006596 | biological_process | polyamine biosynthetic process |
| F | 0016787 | molecular_function | hydrolase activity |
| F | 0016811 | molecular_function | hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides |
| F | 0033388 | biological_process | putrescine biosynthetic process from arginine |
| F | 0050126 | molecular_function | N-carbamoylputrescine amidase activity |
| G | 0006596 | biological_process | polyamine biosynthetic process |
| G | 0016787 | molecular_function | hydrolase activity |
| G | 0016811 | molecular_function | hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides |
| G | 0033388 | biological_process | putrescine biosynthetic process from arginine |
| G | 0050126 | molecular_function | N-carbamoylputrescine amidase activity |
| H | 0006596 | biological_process | polyamine biosynthetic process |
| H | 0016787 | molecular_function | hydrolase activity |
| H | 0016811 | molecular_function | hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides |
| H | 0033388 | biological_process | putrescine biosynthetic process from arginine |
| H | 0050126 | molecular_function | N-carbamoylputrescine amidase activity |
| I | 0006596 | biological_process | polyamine biosynthetic process |
| I | 0016787 | molecular_function | hydrolase activity |
| I | 0016811 | molecular_function | hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides |
| I | 0033388 | biological_process | putrescine biosynthetic process from arginine |
| I | 0050126 | molecular_function | N-carbamoylputrescine amidase activity |
| J | 0006596 | biological_process | polyamine biosynthetic process |
| J | 0016787 | molecular_function | hydrolase activity |
| J | 0016811 | molecular_function | hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides |
| J | 0033388 | biological_process | putrescine biosynthetic process from arginine |
| J | 0050126 | molecular_function | N-carbamoylputrescine amidase activity |
| K | 0006596 | biological_process | polyamine biosynthetic process |
| K | 0016787 | molecular_function | hydrolase activity |
| K | 0016811 | molecular_function | hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides |
| K | 0033388 | biological_process | putrescine biosynthetic process from arginine |
| K | 0050126 | molecular_function | N-carbamoylputrescine amidase activity |
| L | 0006596 | biological_process | polyamine biosynthetic process |
| L | 0016787 | molecular_function | hydrolase activity |
| L | 0016811 | molecular_function | hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides |
| L | 0033388 | biological_process | putrescine biosynthetic process from arginine |
| L | 0050126 | molecular_function | N-carbamoylputrescine amidase activity |
| M | 0006596 | biological_process | polyamine biosynthetic process |
| M | 0016787 | molecular_function | hydrolase activity |
| M | 0016811 | molecular_function | hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides |
| M | 0033388 | biological_process | putrescine biosynthetic process from arginine |
| M | 0050126 | molecular_function | N-carbamoylputrescine amidase activity |
| N | 0006596 | biological_process | polyamine biosynthetic process |
| N | 0016787 | molecular_function | hydrolase activity |
| N | 0016811 | molecular_function | hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides |
| N | 0033388 | biological_process | putrescine biosynthetic process from arginine |
| N | 0050126 | molecular_function | N-carbamoylputrescine amidase activity |
| O | 0006596 | biological_process | polyamine biosynthetic process |
| O | 0016787 | molecular_function | hydrolase activity |
| O | 0016811 | molecular_function | hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides |
| O | 0033388 | biological_process | putrescine biosynthetic process from arginine |
| O | 0050126 | molecular_function | N-carbamoylputrescine amidase activity |
| P | 0006596 | biological_process | polyamine biosynthetic process |
| P | 0016787 | molecular_function | hydrolase activity |
| P | 0016811 | molecular_function | hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides |
| P | 0033388 | biological_process | putrescine biosynthetic process from arginine |
| P | 0050126 | molecular_function | N-carbamoylputrescine amidase activity |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 3 |
| Details | binding site for residue GOL A 401 |
| Chain | Residue |
| A | SER23 |
| A | THR75 |
| A | ARG78 |
| site_id | AC2 |
| Number of Residues | 5 |
| Details | binding site for residue GOL A 402 |
| Chain | Residue |
| A | TYR54 |
| A | TYR130 |
| A | ALA183 |
| A | ILE184 |
| A | GLY185 |
| site_id | AC3 |
| Number of Residues | 6 |
| Details | binding site for residue EDO A 403 |
| Chain | Residue |
| A | ASP286 |
| A | HOH538 |
| C | PRO138 |
| D | HOH583 |
| D | HOH618 |
| A | PRO285 |
| site_id | AC4 |
| Number of Residues | 7 |
| Details | binding site for residue GOL B 401 |
| Chain | Residue |
| A | TRP277 |
| B | ASP126 |
| B | TYR130 |
| B | GLU187 |
| B | HOH505 |
| B | HOH572 |
| B | HOH604 |
| site_id | AC5 |
| Number of Residues | 6 |
| Details | binding site for residue GOL B 402 |
| Chain | Residue |
| A | MET273 |
| A | TRP277 |
| B | SER195 |
| B | HIS198 |
| B | HOH559 |
| B | HOH561 |
| site_id | AC6 |
| Number of Residues | 3 |
| Details | binding site for residue GOL B 403 |
| Chain | Residue |
| B | ALA171 |
| B | LEU172 |
| B | ARG274 |
| site_id | AC7 |
| Number of Residues | 4 |
| Details | binding site for residue GOL B 404 |
| Chain | Residue |
| B | GLU51 |
| B | ARG66 |
| B | HOH551 |
| B | HOH566 |
| site_id | AC8 |
| Number of Residues | 4 |
| Details | binding site for residue GOL B 405 |
| Chain | Residue |
| B | TYR70 |
| B | HOH537 |
| B | HOH571 |
| B | HOH633 |
| site_id | AC9 |
| Number of Residues | 8 |
| Details | binding site for residue GOL C 401 |
| Chain | Residue |
| C | ASP126 |
| C | TRP159 |
| C | GLU187 |
| C | HOH501 |
| C | HOH544 |
| C | HOH599 |
| C | HOH643 |
| D | TRP277 |
| site_id | AD1 |
| Number of Residues | 4 |
| Details | binding site for residue GOL C 402 |
| Chain | Residue |
| C | MET273 |
| C | TRP277 |
| C | HOH615 |
| D | HIS198 |
| site_id | AD2 |
| Number of Residues | 4 |
| Details | binding site for residue GOL C 403 |
| Chain | Residue |
| C | SER23 |
| C | THR75 |
| C | ARG78 |
| C | HOH527 |
| site_id | AD3 |
| Number of Residues | 4 |
| Details | binding site for residue PEG C 404 |
| Chain | Residue |
| C | TYR70 |
| C | ASP115 |
| C | LEU116 |
| C | HOH683 |
| site_id | AD4 |
| Number of Residues | 6 |
| Details | binding site for residue EDO C 405 |
| Chain | Residue |
| C | PRO285 |
| C | ASP286 |
| C | HOH579 |
| C | HOH616 |
| E | HOH632 |
| F | HOH531 |
| site_id | AD5 |
| Number of Residues | 6 |
| Details | binding site for residue GOL D 401 |
| Chain | Residue |
| C | TRP277 |
| D | ASP126 |
| D | TYR130 |
| D | GLU187 |
| D | HOH501 |
| D | HOH648 |
| site_id | AD6 |
| Number of Residues | 6 |
| Details | binding site for residue GOL D 402 |
| Chain | Residue |
| C | ILE193 |
| C | HIS198 |
| D | MET273 |
| D | TRP277 |
| D | HOH542 |
| D | HOH562 |
| site_id | AD7 |
| Number of Residues | 4 |
| Details | binding site for residue GOL D 403 |
| Chain | Residue |
| D | SER23 |
| D | THR75 |
| D | ARG78 |
| D | HOH647 |
| site_id | AD8 |
| Number of Residues | 6 |
| Details | binding site for residue GOL D 404 |
| Chain | Residue |
| C | ARG201 |
| C | HOH602 |
| D | GLN204 |
| D | GLU248 |
| D | ILE249 |
| D | HOH554 |
| site_id | AD9 |
| Number of Residues | 6 |
| Details | binding site for residue EDO D 405 |
| Chain | Residue |
| A | HOH521 |
| C | VAL300 |
| D | PRO285 |
| D | ASP286 |
| D | HOH543 |
| D | HOH679 |
| site_id | AE1 |
| Number of Residues | 6 |
| Details | binding site for residue GOL E 401 |
| Chain | Residue |
| E | ASP126 |
| E | TYR130 |
| E | GLU187 |
| E | HOH517 |
| E | HOH542 |
| F | TRP277 |
| site_id | AE2 |
| Number of Residues | 4 |
| Details | binding site for residue GOL E 402 |
| Chain | Residue |
| E | TRP277 |
| E | HOH524 |
| F | HIS198 |
| E | MET273 |
| site_id | AE3 |
| Number of Residues | 5 |
| Details | binding site for residue GOL E 403 |
| Chain | Residue |
| E | SER23 |
| E | PRO74 |
| E | THR75 |
| E | ARG78 |
| E | HOH588 |
| site_id | AE4 |
| Number of Residues | 1 |
| Details | binding site for residue GOL E 404 |
| Chain | Residue |
| E | GLY113 |
| site_id | AE5 |
| Number of Residues | 9 |
| Details | binding site for residue GOL F 401 |
| Chain | Residue |
| E | TRP277 |
| E | HOH649 |
| F | ASP126 |
| F | TYR130 |
| F | TRP159 |
| F | GLU187 |
| F | HOH518 |
| F | HOH581 |
| F | HOH627 |
| site_id | AE6 |
| Number of Residues | 5 |
| Details | binding site for residue GOL F 402 |
| Chain | Residue |
| E | HIS198 |
| F | MET273 |
| F | TRP277 |
| F | HOH503 |
| F | HOH534 |
| site_id | AE7 |
| Number of Residues | 5 |
| Details | binding site for residue GOL F 403 |
| Chain | Residue |
| F | VAL22 |
| F | SER23 |
| F | PRO74 |
| F | THR75 |
| F | ARG78 |
| site_id | AE8 |
| Number of Residues | 7 |
| Details | binding site for residue GOL G 401 |
| Chain | Residue |
| G | PRO125 |
| G | ASP126 |
| G | TYR130 |
| G | TRP159 |
| G | GLU187 |
| G | HOH540 |
| H | TRP277 |
| site_id | AE9 |
| Number of Residues | 4 |
| Details | binding site for residue GOL G 402 |
| Chain | Residue |
| G | SER195 |
| G | HIS198 |
| H | MET273 |
| H | TRP277 |
| site_id | AF1 |
| Number of Residues | 6 |
| Details | binding site for residue GOL G 403 |
| Chain | Residue |
| G | LYS256 |
| G | GLU257 |
| G | HOH519 |
| G | HOH529 |
| L | LEU32 |
| L | GLU258 |
| site_id | AF2 |
| Number of Residues | 2 |
| Details | binding site for residue PEG G 404 |
| Chain | Residue |
| G | ARG7 |
| G | GLN147 |
| site_id | AF3 |
| Number of Residues | 6 |
| Details | binding site for residue GOL I 401 |
| Chain | Residue |
| I | MET273 |
| I | TRP277 |
| I | HOH514 |
| I | HOH540 |
| I | HOH584 |
| J | HIS198 |
| site_id | AF4 |
| Number of Residues | 4 |
| Details | binding site for residue GOL I 402 |
| Chain | Residue |
| I | SER23 |
| I | PRO74 |
| I | THR75 |
| I | ARG78 |
| site_id | AF5 |
| Number of Residues | 5 |
| Details | binding site for residue EDO I 403 |
| Chain | Residue |
| I | PRO285 |
| I | ASP286 |
| I | HOH532 |
| K | PRO138 |
| L | HOH513 |
| site_id | AF6 |
| Number of Residues | 6 |
| Details | binding site for residue GOL J 401 |
| Chain | Residue |
| I | TRP277 |
| I | HOH565 |
| J | ASP126 |
| J | GLU187 |
| J | HOH527 |
| J | HOH588 |
| site_id | AF7 |
| Number of Residues | 5 |
| Details | binding site for residue GOL J 402 |
| Chain | Residue |
| J | ALA171 |
| J | ARG274 |
| J | PHE280 |
| J | HOH572 |
| J | HOH606 |
| site_id | AF8 |
| Number of Residues | 1 |
| Details | binding site for residue PEG J 403 |
| Chain | Residue |
| J | ILE249 |
| site_id | AF9 |
| Number of Residues | 7 |
| Details | binding site for residue GOL K 401 |
| Chain | Residue |
| K | ASP126 |
| K | TYR130 |
| K | GLU187 |
| K | HOH509 |
| K | HOH597 |
| K | HOH652 |
| L | TRP277 |
| site_id | AG1 |
| Number of Residues | 6 |
| Details | binding site for residue GOL K 402 |
| Chain | Residue |
| K | MET273 |
| K | TRP277 |
| K | HOH525 |
| K | HOH639 |
| L | SER195 |
| L | HIS198 |
| site_id | AG2 |
| Number of Residues | 5 |
| Details | binding site for residue GOL K 403 |
| Chain | Residue |
| K | SER23 |
| K | THR75 |
| K | ARG78 |
| K | HOH502 |
| K | HOH529 |
| site_id | AG3 |
| Number of Residues | 7 |
| Details | binding site for residue EDO K 404 |
| Chain | Residue |
| K | ARG283 |
| K | PRO285 |
| K | ASP286 |
| K | HOH538 |
| K | HOH584 |
| M | HOH631 |
| N | HOH513 |
| site_id | AG4 |
| Number of Residues | 9 |
| Details | binding site for residue GOL L 401 |
| Chain | Residue |
| K | TRP277 |
| L | ASP126 |
| L | TYR130 |
| L | TRP159 |
| L | GLU187 |
| L | HOH507 |
| L | HOH517 |
| L | HOH603 |
| L | HOH677 |
| site_id | AG5 |
| Number of Residues | 5 |
| Details | binding site for residue GOL L 402 |
| Chain | Residue |
| K | HIS198 |
| L | MET273 |
| L | TRP277 |
| L | HOH557 |
| L | HOH560 |
| site_id | AG6 |
| Number of Residues | 6 |
| Details | binding site for residue GOL L 403 |
| Chain | Residue |
| L | SER23 |
| L | THR75 |
| L | ARG78 |
| L | HOH512 |
| L | HOH543 |
| L | HOH549 |
| site_id | AG7 |
| Number of Residues | 2 |
| Details | binding site for residue PEG L 404 |
| Chain | Residue |
| L | LYS81 |
| L | ASP112 |
| site_id | AG8 |
| Number of Residues | 7 |
| Details | binding site for residue EDO L 405 |
| Chain | Residue |
| J | HOH616 |
| K | VAL300 |
| L | ARG283 |
| L | PRO285 |
| L | ASP286 |
| L | HOH514 |
| L | HOH579 |
| site_id | AG9 |
| Number of Residues | 7 |
| Details | binding site for residue GOL M 401 |
| Chain | Residue |
| M | ASP126 |
| M | TYR130 |
| M | TRP159 |
| M | GLU187 |
| M | HOH514 |
| M | HOH595 |
| N | TRP277 |
| site_id | AH1 |
| Number of Residues | 6 |
| Details | binding site for residue GOL M 402 |
| Chain | Residue |
| M | SER195 |
| M | HIS198 |
| M | HOH506 |
| M | HOH596 |
| N | MET273 |
| N | TRP277 |
| site_id | AH2 |
| Number of Residues | 5 |
| Details | binding site for residue GOL M 403 |
| Chain | Residue |
| M | VAL22 |
| M | SER23 |
| M | THR75 |
| M | ARG78 |
| M | HOH524 |
| site_id | AH3 |
| Number of Residues | 8 |
| Details | binding site for residue GOL N 401 |
| Chain | Residue |
| M | TRP277 |
| M | HOH579 |
| N | ASP126 |
| N | TYR130 |
| N | TRP159 |
| N | GLU187 |
| N | HOH518 |
| N | HOH559 |
| site_id | AH4 |
| Number of Residues | 4 |
| Details | binding site for residue GOL N 402 |
| Chain | Residue |
| M | TRP277 |
| N | HIS198 |
| N | HOH515 |
| N | HOH557 |
| site_id | AH5 |
| Number of Residues | 6 |
| Details | binding site for residue GOL N 403 |
| Chain | Residue |
| N | SER23 |
| N | PRO74 |
| N | THR75 |
| N | ARG78 |
| N | HOH578 |
| N | HOH609 |
| site_id | AH6 |
| Number of Residues | 1 |
| Details | binding site for residue GOL N 404 |
| Chain | Residue |
| N | HOH509 |
| site_id | AH7 |
| Number of Residues | 8 |
| Details | binding site for residue GOL O 401 |
| Chain | Residue |
| O | ASP126 |
| O | TRP159 |
| O | GLU187 |
| O | HOH533 |
| O | HOH580 |
| O | HOH619 |
| P | TRP277 |
| P | HOH527 |
| site_id | AH8 |
| Number of Residues | 5 |
| Details | binding site for residue GOL P 401 |
| Chain | Residue |
| O | SER195 |
| O | HIS198 |
| P | MET273 |
| P | TRP277 |
| P | HOH509 |
