5G05

Cryo-EM structure of combined apo phosphorylated APC

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005634	cellular_component	nucleus
A	0005654	cellular_component	nucleoplasm
A	0005680	cellular_component	anaphase-promoting complex
A	0005829	cellular_component	cytosol
A	0007049	biological_process	cell cycle
A	0007091	biological_process	metaphase/anaphase transition of mitotic cell cycle
A	0007346	biological_process	regulation of mitotic cell cycle
A	0016567	biological_process	protein ubiquitination
A	0031145	biological_process	anaphase-promoting complex-dependent catabolic process
A	0051301	biological_process	cell division
A	0051445	biological_process	regulation of meiotic cell cycle
A	0060090	molecular_function	molecular adaptor activity
A	0070979	biological_process	protein K11-linked ubiquitination
B	0000278	biological_process	mitotic cell cycle
B	0004842	molecular_function	ubiquitin-protein transferase activity
B	0005515	molecular_function	protein binding
B	0005634	cellular_component	nucleus
B	0005654	cellular_component	nucleoplasm
B	0005680	cellular_component	anaphase-promoting complex
B	0005730	cellular_component	nucleolus
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0006511	biological_process	ubiquitin-dependent protein catabolic process
B	0007049	biological_process	cell cycle
B	0007346	biological_process	regulation of mitotic cell cycle
B	0008270	molecular_function	zinc ion binding
B	0016567	biological_process	protein ubiquitination
B	0031145	biological_process	anaphase-promoting complex-dependent catabolic process
B	0034450	molecular_function	ubiquitin-ubiquitin ligase activity
B	0045842	biological_process	positive regulation of mitotic metaphase/anaphase transition
B	0046872	molecular_function	metal ion binding
B	0051301	biological_process	cell division
B	0051445	biological_process	regulation of meiotic cell cycle
B	0061630	molecular_function	ubiquitin protein ligase activity
B	0070979	biological_process	protein K11-linked ubiquitination
B	0097602	molecular_function	cullin family protein binding
C	0000278	biological_process	mitotic cell cycle
C	0004842	molecular_function	ubiquitin-protein transferase activity
C	0005515	molecular_function	protein binding
C	0005654	cellular_component	nucleoplasm
C	0005680	cellular_component	anaphase-promoting complex
C	0005829	cellular_component	cytosol
C	0006511	biological_process	ubiquitin-dependent protein catabolic process
C	0007049	biological_process	cell cycle
C	0007080	biological_process	mitotic metaphase chromosome alignment
C	0007091	biological_process	metaphase/anaphase transition of mitotic cell cycle
C	0007096	biological_process	regulation of exit from mitosis
C	0007346	biological_process	regulation of mitotic cell cycle
C	0016567	biological_process	protein ubiquitination
C	0030071	biological_process	regulation of mitotic metaphase/anaphase transition
C	0031145	biological_process	anaphase-promoting complex-dependent catabolic process
C	0045842	biological_process	positive regulation of mitotic metaphase/anaphase transition
C	0051301	biological_process	cell division
C	0051445	biological_process	regulation of meiotic cell cycle
C	0070979	biological_process	protein K11-linked ubiquitination
D	0005515	molecular_function	protein binding
D	0005654	cellular_component	nucleoplasm
D	0005680	cellular_component	anaphase-promoting complex
D	0005829	cellular_component	cytosol
D	0007049	biological_process	cell cycle
D	0007346	biological_process	regulation of mitotic cell cycle
D	0031145	biological_process	anaphase-promoting complex-dependent catabolic process
D	0051301	biological_process	cell division
D	0051445	biological_process	regulation of meiotic cell cycle
D	0090266	biological_process	regulation of mitotic cell cycle spindle assembly checkpoint
E	0000775	cellular_component	chromosome, centromeric region
E	0000776	cellular_component	kinetochore
E	0005515	molecular_function	protein binding
E	0005634	cellular_component	nucleus
E	0005654	cellular_component	nucleoplasm
E	0005680	cellular_component	anaphase-promoting complex
E	0005694	cellular_component	chromosome
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0007049	biological_process	cell cycle
E	0007346	biological_process	regulation of mitotic cell cycle
E	0016567	biological_process	protein ubiquitination
E	0031145	biological_process	anaphase-promoting complex-dependent catabolic process
E	0051301	biological_process	cell division
E	0051445	biological_process	regulation of meiotic cell cycle
F	0005515	molecular_function	protein binding
F	0005634	cellular_component	nucleus
F	0005654	cellular_component	nucleoplasm
F	0005680	cellular_component	anaphase-promoting complex
F	0005737	cellular_component	cytoplasm
F	0005813	cellular_component	centrosome
F	0005819	cellular_component	spindle
F	0005829	cellular_component	cytosol
F	0005856	cellular_component	cytoskeleton
F	0007091	biological_process	metaphase/anaphase transition of mitotic cell cycle
F	0007346	biological_process	regulation of mitotic cell cycle
F	0016567	biological_process	protein ubiquitination
F	0019903	molecular_function	protein phosphatase binding
F	0031145	biological_process	anaphase-promoting complex-dependent catabolic process
F	0051301	biological_process	cell division
F	0051445	biological_process	regulation of meiotic cell cycle
F	0070979	biological_process	protein K11-linked ubiquitination
F	0072686	cellular_component	mitotic spindle
G	0005515	molecular_function	protein binding
G	0005634	cellular_component	nucleus
G	0005654	cellular_component	nucleoplasm
G	0005680	cellular_component	anaphase-promoting complex
G	0005829	cellular_component	cytosol
G	0007049	biological_process	cell cycle
G	0007346	biological_process	regulation of mitotic cell cycle
G	0016567	biological_process	protein ubiquitination
G	0031145	biological_process	anaphase-promoting complex-dependent catabolic process
G	0051301	biological_process	cell division
G	0051445	biological_process	regulation of meiotic cell cycle
G	0070979	biological_process	protein K11-linked ubiquitination
H	0005515	molecular_function	protein binding
H	0005634	cellular_component	nucleus
H	0005654	cellular_component	nucleoplasm
H	0005680	cellular_component	anaphase-promoting complex
H	0005737	cellular_component	cytoplasm
H	0005813	cellular_component	centrosome
H	0005819	cellular_component	spindle
H	0005829	cellular_component	cytosol
H	0005856	cellular_component	cytoskeleton
H	0007091	biological_process	metaphase/anaphase transition of mitotic cell cycle
H	0007346	biological_process	regulation of mitotic cell cycle
H	0016567	biological_process	protein ubiquitination
H	0019903	molecular_function	protein phosphatase binding
H	0031145	biological_process	anaphase-promoting complex-dependent catabolic process
H	0051301	biological_process	cell division
H	0051445	biological_process	regulation of meiotic cell cycle
H	0070979	biological_process	protein K11-linked ubiquitination
H	0072686	cellular_component	mitotic spindle
I	0004842	molecular_function	ubiquitin-protein transferase activity
I	0005515	molecular_function	protein binding
I	0005634	cellular_component	nucleus
I	0005654	cellular_component	nucleoplasm
I	0005680	cellular_component	anaphase-promoting complex
I	0005829	cellular_component	cytosol
I	0007049	biological_process	cell cycle
I	0007346	biological_process	regulation of mitotic cell cycle
I	0016567	biological_process	protein ubiquitination
I	0019903	molecular_function	protein phosphatase binding
I	0030071	biological_process	regulation of mitotic metaphase/anaphase transition
I	0031145	biological_process	anaphase-promoting complex-dependent catabolic process
I	0034399	cellular_component	nuclear periphery
I	0051301	biological_process	cell division
I	0051445	biological_process	regulation of meiotic cell cycle
I	0070979	biological_process	protein K11-linked ubiquitination
J	0005515	molecular_function	protein binding
J	0005634	cellular_component	nucleus
J	0005654	cellular_component	nucleoplasm
J	0005680	cellular_component	anaphase-promoting complex
J	0005737	cellular_component	cytoplasm
J	0005813	cellular_component	centrosome
J	0005819	cellular_component	spindle
J	0005829	cellular_component	cytosol
J	0005856	cellular_component	cytoskeleton
J	0007049	biological_process	cell cycle
J	0007346	biological_process	regulation of mitotic cell cycle
J	0016567	biological_process	protein ubiquitination
J	0031145	biological_process	anaphase-promoting complex-dependent catabolic process
J	0045842	biological_process	positive regulation of mitotic metaphase/anaphase transition
J	0051301	biological_process	cell division
J	0051445	biological_process	regulation of meiotic cell cycle
J	0070979	biological_process	protein K11-linked ubiquitination
J	0072686	cellular_component	mitotic spindle
K	0005515	molecular_function	protein binding
K	0005634	cellular_component	nucleus
K	0005654	cellular_component	nucleoplasm
K	0005680	cellular_component	anaphase-promoting complex
K	0005737	cellular_component	cytoplasm
K	0005813	cellular_component	centrosome
K	0005819	cellular_component	spindle
K	0005829	cellular_component	cytosol
K	0005856	cellular_component	cytoskeleton
K	0007049	biological_process	cell cycle
K	0007346	biological_process	regulation of mitotic cell cycle
K	0016567	biological_process	protein ubiquitination
K	0031145	biological_process	anaphase-promoting complex-dependent catabolic process
K	0045842	biological_process	positive regulation of mitotic metaphase/anaphase transition
K	0051301	biological_process	cell division
K	0051445	biological_process	regulation of meiotic cell cycle
K	0070979	biological_process	protein K11-linked ubiquitination
K	0072686	cellular_component	mitotic spindle
L	0005515	molecular_function	protein binding
L	0005654	cellular_component	nucleoplasm
L	0005680	cellular_component	anaphase-promoting complex
L	0005737	cellular_component	cytoplasm
L	0005829	cellular_component	cytosol
L	0007049	biological_process	cell cycle
L	0007346	biological_process	regulation of mitotic cell cycle
L	0016567	biological_process	protein ubiquitination
L	0031145	biological_process	anaphase-promoting complex-dependent catabolic process
L	0051301	biological_process	cell division
L	0051445	biological_process	regulation of meiotic cell cycle
L	0070979	biological_process	protein K11-linked ubiquitination
M	0005634	cellular_component	nucleus
M	0005680	cellular_component	anaphase-promoting complex
M	0007049	biological_process	cell cycle
M	0016567	biological_process	protein ubiquitination
M	0051301	biological_process	cell division
M	0070979	biological_process	protein K11-linked ubiquitination
N	0005515	molecular_function	protein binding
N	0005654	cellular_component	nucleoplasm
N	0005680	cellular_component	anaphase-promoting complex
N	0005829	cellular_component	cytosol
N	0006511	biological_process	ubiquitin-dependent protein catabolic process
N	0007049	biological_process	cell cycle
N	0007091	biological_process	metaphase/anaphase transition of mitotic cell cycle
N	0007346	biological_process	regulation of mitotic cell cycle
N	0007399	biological_process	nervous system development
N	0010629	biological_process	negative regulation of gene expression
N	0016567	biological_process	protein ubiquitination
N	0030154	biological_process	cell differentiation
N	0031145	biological_process	anaphase-promoting complex-dependent catabolic process
N	0031461	cellular_component	cullin-RING ubiquitin ligase complex
N	0031625	molecular_function	ubiquitin protein ligase binding
N	0031915	biological_process	positive regulation of synaptic plasticity
N	0045773	biological_process	positive regulation of axon extension
N	0050775	biological_process	positive regulation of dendrite morphogenesis
N	0051301	biological_process	cell division
N	0051445	biological_process	regulation of meiotic cell cycle
N	0070979	biological_process	protein K11-linked ubiquitination
N	0090129	biological_process	positive regulation of synapse maturation
O	0005634	cellular_component	nucleus
O	0005654	cellular_component	nucleoplasm
O	0005680	cellular_component	anaphase-promoting complex
O	0005737	cellular_component	cytoplasm
O	0005819	cellular_component	spindle
O	0005829	cellular_component	cytosol
O	0005856	cellular_component	cytoskeleton
O	0007049	biological_process	cell cycle
O	0007346	biological_process	regulation of mitotic cell cycle
O	0016567	biological_process	protein ubiquitination
O	0019903	molecular_function	protein phosphatase binding
O	0031145	biological_process	anaphase-promoting complex-dependent catabolic process
O	0045842	biological_process	positive regulation of mitotic metaphase/anaphase transition
O	0051301	biological_process	cell division
O	0051445	biological_process	regulation of meiotic cell cycle
O	0070979	biological_process	protein K11-linked ubiquitination
P	0000278	biological_process	mitotic cell cycle
P	0004842	molecular_function	ubiquitin-protein transferase activity
P	0005515	molecular_function	protein binding
P	0005654	cellular_component	nucleoplasm
P	0005680	cellular_component	anaphase-promoting complex
P	0005829	cellular_component	cytosol
P	0006511	biological_process	ubiquitin-dependent protein catabolic process
P	0007049	biological_process	cell cycle
P	0007080	biological_process	mitotic metaphase chromosome alignment
P	0007091	biological_process	metaphase/anaphase transition of mitotic cell cycle
P	0007096	biological_process	regulation of exit from mitosis
P	0007346	biological_process	regulation of mitotic cell cycle
P	0016567	biological_process	protein ubiquitination
P	0030071	biological_process	regulation of mitotic metaphase/anaphase transition
P	0031145	biological_process	anaphase-promoting complex-dependent catabolic process
P	0045842	biological_process	positive regulation of mitotic metaphase/anaphase transition
P	0051301	biological_process	cell division
P	0051445	biological_process	regulation of meiotic cell cycle
P	0070979	biological_process	protein K11-linked ubiquitination
W	0005515	molecular_function	protein binding
W	0005634	cellular_component	nucleus
W	0005654	cellular_component	nucleoplasm
W	0005680	cellular_component	anaphase-promoting complex
W	0005829	cellular_component	cytosol
W	0007049	biological_process	cell cycle
W	0007346	biological_process	regulation of mitotic cell cycle
W	0016567	biological_process	protein ubiquitination
W	0031145	biological_process	anaphase-promoting complex-dependent catabolic process
W	0051301	biological_process	cell division
W	0051445	biological_process	regulation of meiotic cell cycle
W	0070979	biological_process	protein K11-linked ubiquitination
X	0000792	cellular_component	heterochromatin
X	0005634	cellular_component	nucleus
X	0005654	cellular_component	nucleoplasm
X	0005680	cellular_component	anaphase-promoting complex
X	0005737	cellular_component	cytoplasm
X	0005819	cellular_component	spindle
X	0005829	cellular_component	cytosol
X	0005856	cellular_component	cytoskeleton
X	0007049	biological_process	cell cycle
X	0007346	biological_process	regulation of mitotic cell cycle
X	0007420	biological_process	brain development
X	0015630	cellular_component	microtubule cytoskeleton
X	0016567	biological_process	protein ubiquitination
X	0019903	molecular_function	protein phosphatase binding
X	0031145	biological_process	anaphase-promoting complex-dependent catabolic process
X	0045842	biological_process	positive regulation of mitotic metaphase/anaphase transition
X	0051301	biological_process	cell division
X	0051445	biological_process	regulation of meiotic cell cycle
X	0070979	biological_process	protein K11-linked ubiquitination
X	0140767	molecular_function	enzyme-substrate adaptor activity
Y	0000792	cellular_component	heterochromatin
Y	0005634	cellular_component	nucleus
Y	0005654	cellular_component	nucleoplasm
Y	0005680	cellular_component	anaphase-promoting complex
Y	0005737	cellular_component	cytoplasm
Y	0005819	cellular_component	spindle
Y	0005829	cellular_component	cytosol
Y	0005856	cellular_component	cytoskeleton
Y	0007049	biological_process	cell cycle
Y	0007346	biological_process	regulation of mitotic cell cycle
Y	0007420	biological_process	brain development
Y	0015630	cellular_component	microtubule cytoskeleton
Y	0016567	biological_process	protein ubiquitination
Y	0019903	molecular_function	protein phosphatase binding
Y	0031145	biological_process	anaphase-promoting complex-dependent catabolic process
Y	0045842	biological_process	positive regulation of mitotic metaphase/anaphase transition
Y	0051301	biological_process	cell division
Y	0051445	biological_process	regulation of meiotic cell cycle
Y	0070979	biological_process	protein K11-linked ubiquitination
Y	0140767	molecular_function	enzyme-substrate adaptor activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN B 1085

Chain	Residue
B	CYS23
B	CYS26
B	HIS56
B	CYS59

---

site_id	AC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN B 1086

Chain	Residue
B	CYS51
B	HIS53
B	CYS73
B	CYS76

---

site_id	AC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN B 1087

Chain	Residue
B	CYS37
B	CYS44
B	HIS58
B	CYS34

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	MOD_RES: N6-acetyllysine => ECO:0000250|UniProtKB:Q9WVM3

Chain	Residue	Details
X	LYS263
Y	LYS263
J	SER595
K	SER112
K	SER490
K	SER595

---

site_id	SWS_FT_FI2
Number of Residues	1
Details	MOD_RES: Phosphothreonine => ECO:0007744|PubMed:23186163

Chain	Residue	Details
O	THR232
B	CYS59
B	CYS73
B	CYS76
K	SER560
F	THR313
F	THR430
H	THR209
H	THR244
H	THR313
H	THR430
B	HIS58

---

site_id	SWS_FT_FI3
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:19690332

Chain	Residue	Details
N	SER470
K	THR581
H	SER291
H	SER435

---

site_id	SWS_FT_FI4
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0000269|PubMed:14657031, ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:23186163

Chain	Residue	Details
N	SER534
K	THR599
H	SER339
H	SER438

---

site_id	SWS_FT_FI5
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:Q8BZQ7

Chain	Residue	Details
N	SER697
H	THR366
I	LYS798

---

site_id	SWS_FT_FI6
Number of Residues	1
Details	MOD_RES: Phosphotyrosine => ECO:0000250|UniProtKB:Q8BZQ7

Chain	Residue	Details
N	TYR810
H	SER379

---

site_id	SWS_FT_FI7
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:20068231

Chain	Residue	Details
F	SER386
H	SER386

---

site_id	SWS_FT_FI8
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0000269|PubMed:14657031, ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:23186163

Chain	Residue	Details
F	SER426
H	SER426

---

site_id	SWS_FT_FI9
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0000269|PubMed:22139841

Chain	Residue	Details
F	SER821
H	SER821

---

site_id	SWS_FT_FI10
Number of Residues	2
Details	MOD_RES: Phosphothreonine => ECO:0007744|PubMed:16964243, ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:19690332, ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:21406692, ECO:0007744|PubMed:23186163, ECO:0007744|PubMed:24275569

Chain	Residue	Details
C	THR596
P	THR596

---

site_id	SWS_FT_FI11
Number of Residues	4
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744|PubMed:25218447, ECO:0007744|PubMed:28112733

Chain	Residue	Details
C	LYS147
P	LYS147

---

site_id	SWS_FT_FI12
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:18691976, ECO:0007744|PubMed:19690332, ECO:0007744|PubMed:20068231

Chain	Residue	Details
A	SER555

---

site_id	SWS_FT_FI13
Number of Residues	1
Details	MOD_RES: Phosphotyrosine => ECO:0000269|PubMed:14657031

Chain	Residue	Details
A	TYR571

---

site_id	SWS_FT_FI14
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:20068231

Chain	Residue	Details
A	SER686

---

site_id	SWS_FT_FI15
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0000269|PubMed:14657031, ECO:0007744|PubMed:19690332, ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:21406692, ECO:0007744|PubMed:23186163, ECO:0007744|PubMed:24275569

Chain	Residue	Details
A	SER688

---