5FYD

Structural and biochemical insights into 7beta-hydroxysteroid dehydrogenase stereoselectivity

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0016042	biological_process	lipid catabolic process
A	0016491	molecular_function	oxidoreductase activity
A	0030573	biological_process	bile acid catabolic process
A	0047022	molecular_function	7-beta-hydroxysteroid dehydrogenase (NADP+) activity
B	0000166	molecular_function	nucleotide binding
B	0016042	biological_process	lipid catabolic process
B	0016491	molecular_function	oxidoreductase activity
B	0030573	biological_process	bile acid catabolic process
B	0047022	molecular_function	7-beta-hydroxysteroid dehydrogenase (NADP+) activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	10
Details	BINDING SITE FOR RESIDUE GOL A 1260

Chain	Residue
A	GLU74
B	HOH2078
A	TRP105
A	HOH2062
A	HOH2063
B	GLU74
B	PHE77
B	LYS121
B	HIS124
B	HIS125

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	ACT_SITE: Proton acceptor => ECO:0000305|PubMed:27006087

Chain	Residue	Details
A	TYR156
B	TYR156

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site_id	SWS_FT_FI2
Number of Residues	8
Details	BINDING: BINDING => ECO:0000269|PubMed:28471355, ECO:0007744|PDB:5GT9

Chain	Residue	Details
A	THR17
A	ARG40
A	ASP66
A	SER240
B	THR17
B	ARG40
B	ASP66
B	SER240

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site_id	SWS_FT_FI3
Number of Residues	2
Details	SITE: Transition state stabilizer => ECO:0000305|PubMed:27006087

Chain	Residue	Details
A	SER143
B	SER143

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site_id	SWS_FT_FI4
Number of Residues	2
Details	SITE: Lowers pKa of active site Tyr => ECO:0000250|UniProtKB:P0AET8

Chain	Residue	Details
A	LYS160
B	LYS160

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