5FIP
Discovery and characterization of a novel thermostable and highly halotolerant GH5 cellulase from an Icelandic hot spring isolate
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004553 | molecular_function | hydrolase activity, hydrolyzing O-glycosyl compounds |
| A | 0005975 | biological_process | carbohydrate metabolic process |
| A | 0008810 | molecular_function | cellulase activity |
| A | 0030245 | biological_process | cellulose catabolic process |
| A | 0071704 | biological_process | organic substance metabolic process |
| B | 0004553 | molecular_function | hydrolase activity, hydrolyzing O-glycosyl compounds |
| B | 0005975 | biological_process | carbohydrate metabolic process |
| B | 0008810 | molecular_function | cellulase activity |
| B | 0030245 | biological_process | cellulose catabolic process |
| B | 0071704 | biological_process | organic substance metabolic process |
| C | 0004553 | molecular_function | hydrolase activity, hydrolyzing O-glycosyl compounds |
| C | 0005975 | biological_process | carbohydrate metabolic process |
| C | 0008810 | molecular_function | cellulase activity |
| C | 0030245 | biological_process | cellulose catabolic process |
| C | 0071704 | biological_process | organic substance metabolic process |
| D | 0004553 | molecular_function | hydrolase activity, hydrolyzing O-glycosyl compounds |
| D | 0005975 | biological_process | carbohydrate metabolic process |
| D | 0008810 | molecular_function | cellulase activity |
| D | 0030245 | biological_process | cellulose catabolic process |
| D | 0071704 | biological_process | organic substance metabolic process |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE PEG A 1381 |
| Chain | Residue |
| A | ARG222 |
| A | GLU227 |
| A | ASP251 |
| A | LYS252 |
| A | ASN253 |
| site_id | AC2 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE PEG A 1382 |
| Chain | Residue |
| A | HOH2120 |
| A | GLU197 |
| A | SER198 |
| A | SER198 |
| A | HOH2120 |
| site_id | AC3 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE PEG A 1383 |
| Chain | Residue |
| A | HOH2135 |
| A | HOH2197 |
| A | HOH2198 |
| site_id | AC4 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE PEG A 1384 |
| Chain | Residue |
| A | GLU316 |
| A | ASN319 |
| A | ARG369 |
| site_id | AC5 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE PEG A 1385 |
| Chain | Residue |
| A | TRP91 |
| A | LYS333 |
| A | IPA1394 |
| site_id | AC6 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE PEG A 1386 |
| Chain | Residue |
| A | PRO306 |
| A | TYR307 |
| A | LEU308 |
| A | ASN309 |
| A | GLU310 |
| site_id | AC7 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE PEG A 1387 |
| Chain | Residue |
| A | TRP268 |
| A | LYS273 |
| A | GLU281 |
| A | PHE317 |
| A | ASN321 |
| A | HOH2200 |
| A | HOH2201 |
| C | ARG68 |
| C | PGE1382 |
| site_id | AC8 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE PGE A 1388 |
| Chain | Residue |
| A | ILE62 |
| A | LYS69 |
| A | LYS252 |
| A | GLY287 |
| A | HOH2165 |
| A | HOH2203 |
| site_id | AC9 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE PGE A 1389 |
| Chain | Residue |
| A | ILE65 |
| A | ARG68 |
| A | SER320 |
| A | ASN322 |
| A | HOH2025 |
| A | HOH2204 |
| C | GLU313 |
| site_id | BC1 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE EDO A 1390 |
| Chain | Residue |
| A | LYS169 |
| A | LYS173 |
| site_id | BC2 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE EDO A 1391 |
| Chain | Residue |
| A | HIS75 |
| A | HOH2037 |
| B | VAL353 |
| B | ALA355 |
| site_id | BC3 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE EDO A 1392 |
| Chain | Residue |
| A | LYS106 |
| A | LYS143 |
| A | ASN144 |
| site_id | BC4 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE EDO A 1393 |
| Chain | Residue |
| A | ASP159 |
| A | ASN161 |
| A | ASP199 |
| A | LYS209 |
| site_id | BC5 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE IPA A 1394 |
| Chain | Residue |
| A | HIS87 |
| A | TRP328 |
| A | LYS333 |
| A | GLU335 |
| A | PEG1385 |
| site_id | BC6 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE IPA A 1395 |
| Chain | Residue |
| A | ALA265 |
| A | SER266 |
| A | GLU269 |
| A | HIS275 |
| A | ILE276 |
| A | HOH2150 |
| site_id | BC7 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE PEG B 1382 |
| Chain | Residue |
| B | ASN139 |
| B | LYS178 |
| B | PEG1383 |
| site_id | BC8 |
| Number of Residues | 1 |
| Details | BINDING SITE FOR RESIDUE PEG B 1383 |
| Chain | Residue |
| B | PEG1382 |
| site_id | BC9 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE PGE B 1384 |
| Chain | Residue |
| B | GLU192 |
| B | TRP237 |
| B | TYR261 |
| B | GLU294 |
| B | ALA300 |
| B | EDO1388 |
| B | IPA1399 |
| site_id | CC1 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE EDO B 1385 |
| Chain | Residue |
| A | GLY351 |
| B | PRO54 |
| B | SER55 |
| B | LEU56 |
| B | HIS75 |
| site_id | CC2 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE EDO B 1386 |
| Chain | Residue |
| B | LYS169 |
| B | LYS173 |
| site_id | CC3 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE EDO B 1387 |
| Chain | Residue |
| B | TRP91 |
| B | LYS333 |
| B | IPA1399 |
| B | HOH2172 |
| site_id | CC4 |
| Number of Residues | 1 |
| Details | BINDING SITE FOR RESIDUE EDO B 1388 |
| Chain | Residue |
| B | PGE1384 |
| site_id | CC5 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE EDO B 1389 |
| Chain | Residue |
| B | LYS69 |
| B | HOH2017 |
| site_id | CC6 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE EDO B 1390 |
| Chain | Residue |
| B | PRO248 |
| B | GLY287 |
| site_id | CC7 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE EDO B 1391 |
| Chain | Residue |
| B | TYR124 |
| B | SER125 |
| site_id | CC8 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE EDO B 1392 |
| Chain | Residue |
| B | THR262 |
| B | GLY263 |
| B | ALA265 |
| B | SER266 |
| site_id | CC9 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE PG6 B 1393 |
| Chain | Residue |
| B | ILE65 |
| B | ARG68 |
| B | SER320 |
| B | ASN322 |
| B | HOH2024 |
| D | TRP268 |
| D | GLU313 |
| D | GLU316 |
| site_id | DC1 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE IPA B 1394 |
| Chain | Residue |
| B | ALA265 |
| B | SER266 |
| B | GLU269 |
| B | HIS275 |
| B | ILE276 |
| B | HOH2139 |
| site_id | DC2 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE IPA B 1395 |
| Chain | Residue |
| A | LYS209 |
| A | HOH2128 |
| B | LYS211 |
| B | GLU215 |
| B | ASP250 |
| site_id | DC3 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE IPA B 1396 |
| Chain | Residue |
| B | GLU316 |
| B | ARG369 |
| B | HOH2165 |
| site_id | DC4 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE IPA B 1397 |
| Chain | Residue |
| A | HOH2009 |
| B | ARG367 |
| site_id | DC5 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE IPA B 1399 |
| Chain | Residue |
| B | HIS87 |
| B | TRP328 |
| B | LYS333 |
| B | GLU335 |
| B | PGE1384 |
| B | EDO1387 |
| site_id | DC6 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE PEG C 1381 |
| Chain | Residue |
| C | TRP91 |
| C | PHE92 |
| C | LYS333 |
| site_id | DC7 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE PGE C 1382 |
| Chain | Residue |
| A | TRP268 |
| A | GLU313 |
| A | PEG1387 |
| A | HOH2202 |
| C | ILE65 |
| C | ARG68 |
| C | SER320 |
| C | ASN322 |
| site_id | DC8 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE EDO C 1383 |
| Chain | Residue |
| C | GLU316 |
| C | ASN319 |
| site_id | DC9 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE IPA C 1384 |
| Chain | Residue |
| C | ALA265 |
| C | SER266 |
| C | GLU269 |
| C | HIS275 |
| C | ILE276 |
| site_id | EC1 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE PEG D 1384 |
| Chain | Residue |
| D | LYS69 |
| D | LYS252 |
| D | HOH2064 |
| site_id | EC2 |
| Number of Residues | 1 |
| Details | BINDING SITE FOR RESIDUE EDO D 1385 |
| Chain | Residue |
| A | ASP127 |
| site_id | EC3 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE EDO D 1386 |
| Chain | Residue |
| D | GLU316 |
| D | ASN319 |
| D | ARG369 |
| site_id | EC4 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE EDO D 1387 |
| Chain | Residue |
| B | HOH2146 |
| D | LYS69 |
| D | GLY287 |
| site_id | EC5 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE EDO D 1388 |
| Chain | Residue |
| D | ASN139 |
| D | LYS178 |
| site_id | EC6 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE EDO D 1389 |
| Chain | Residue |
| D | ASN272 |
| D | HOH2057 |
| site_id | EC7 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE PG4 D 1390 |
| Chain | Residue |
| B | TRP268 |
| B | GLU313 |
| D | ILE65 |
| D | ARG68 |
| D | ASN319 |
| D | SER320 |
| D | ASN322 |
| site_id | EC8 |
| Number of Residues | 10 |
| Details | BINDING SITE FOR RESIDUE IPA D 1391 |
| Chain | Residue |
| A | THR126 |
| A | PRO128 |
| D | ALA265 |
| D | SER266 |
| D | GLU269 |
| D | GLY274 |
| D | HIS275 |
| D | ILE276 |
| D | HOH2054 |
| D | HOH2056 |
| site_id | EC9 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE IPA D 1392 |
| Chain | Residue |
| B | ARG278 |
| D | LYS282 |
| D | ASN286 |
| D | HOH2089 |
| site_id | FC1 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE IPA D 1393 |
| Chain | Residue |
| B | GLU281 |
| B | HOH2023 |
| B | HOH2025 |
| D | LYS273 |
| D | GLU281 |
| D | PHE317 |
| D | ASN321 |
| site_id | FC2 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE IPA D 1394 |
| Chain | Residue |
| B | ASN286 |
| D | ARG278 |
| D | LYS282 |
| site_id | FC3 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE CL A 1396 |
| Chain | Residue |
| A | ARG369 |
| A | PRO374 |
| A | TYR375 |
| site_id | FC4 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE CL C 1385 |
| Chain | Residue |
| C | ARG369 |
| C | TYR375 |
| site_id | FC5 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE CL B 1400 |
| Chain | Residue |
| B | ARG369 |
| B | TYR375 |
| site_id | FC6 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE CL B 1401 |
| Chain | Residue |
| B | TYR307 |
| B | LEU308 |
| B | ASN309 |
| B | GLU310 |
| B | HOH2163 |
| site_id | FC7 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE CL A 1400 |
| Chain | Residue |
| A | ARG278 |
| A | HOH2157 |
| site_id | FC8 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE CL A 1397 |
| Chain | Residue |
| A | GLU197 |
| A | THR201 |
| A | ASN202 |
| site_id | FC9 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE CL D 1395 |
| Chain | Residue |
| D | ARG369 |
| D | TYR375 |
Functional Information from PROSITE/UniProt
| site_id | PS00659 |
| Number of Residues | 10 |
| Details | GLYCOSYL_HYDROL_F5 Glycosyl hydrolases family 5 signature. IIYELANEPN |
| Chain | Residue | Details |
| A | ILE185-ASN194 |
