5FAY
Y208F mutant of choline TMA-lyase
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003824 | molecular_function | catalytic activity |
| A | 0005829 | cellular_component | cytosol |
| A | 0016829 | molecular_function | lyase activity |
| A | 0016840 | molecular_function | carbon-nitrogen lyase activity |
| A | 0033265 | molecular_function | choline binding |
| A | 0042426 | biological_process | choline catabolic process |
| A | 0042803 | molecular_function | protein homodimerization activity |
| A | 0120525 | molecular_function | choline trimethylamine lyase activity |
| B | 0003824 | molecular_function | catalytic activity |
| B | 0005829 | cellular_component | cytosol |
| B | 0016829 | molecular_function | lyase activity |
| B | 0016840 | molecular_function | carbon-nitrogen lyase activity |
| B | 0033265 | molecular_function | choline binding |
| B | 0042426 | biological_process | choline catabolic process |
| B | 0042803 | molecular_function | protein homodimerization activity |
| B | 0120525 | molecular_function | choline trimethylamine lyase activity |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 6 |
| Details | binding site for residue NA A 901 |
| Chain | Residue |
| A | SER746 |
| A | MET748 |
| A | HOH1415 |
| A | HOH1572 |
| A | HOH1757 |
| B | HOH1672 |
| site_id | AC2 |
| Number of Residues | 7 |
| Details | binding site for residue MLI A 902 |
| Chain | Residue |
| A | HOH1319 |
| A | HOH1406 |
| A | HOH1482 |
| A | HOH1712 |
| A | ARG572 |
| A | LEU689 |
| A | HOH1015 |
| site_id | AC3 |
| Number of Residues | 6 |
| Details | binding site for residue MLI A 903 |
| Chain | Residue |
| A | ARG238 |
| A | THR633 |
| A | GLN636 |
| A | HOH1047 |
| A | HOH1102 |
| A | HOH1338 |
| site_id | AC4 |
| Number of Residues | 5 |
| Details | binding site for residue MLI A 904 |
| Chain | Residue |
| A | LYS102 |
| A | ARG105 |
| A | HOH1074 |
| A | HOH1528 |
| A | HOH1705 |
| site_id | AC5 |
| Number of Residues | 9 |
| Details | binding site for residue MLI A 905 |
| Chain | Residue |
| A | GLY49 |
| A | SER50 |
| A | HIS51 |
| A | HOH1011 |
| A | HOH1019 |
| A | HOH1040 |
| A | HOH1098 |
| A | HOH1122 |
| B | LYS71 |
| site_id | AC6 |
| Number of Residues | 7 |
| Details | binding site for residue MLI A 906 |
| Chain | Residue |
| A | HIS519 |
| A | ASP535 |
| A | LEU536 |
| A | HOH1023 |
| A | HOH1026 |
| A | HOH1103 |
| A | HOH1208 |
| site_id | AC7 |
| Number of Residues | 6 |
| Details | binding site for residue NA A 907 |
| Chain | Residue |
| A | GLU242 |
| A | LEU244 |
| A | HOH1018 |
| A | HOH1225 |
| A | HOH1617 |
| A | HOH1702 |
| site_id | AC8 |
| Number of Residues | 9 |
| Details | binding site for residue CHT A 908 |
| Chain | Residue |
| A | PHE208 |
| A | ASP216 |
| A | THR334 |
| A | GLY488 |
| A | CYS489 |
| A | GLU491 |
| A | THR502 |
| A | TYR506 |
| A | LEU698 |
| site_id | AC9 |
| Number of Residues | 6 |
| Details | binding site for residue NA B 901 |
| Chain | Residue |
| A | HOH1656 |
| B | SER746 |
| B | MET748 |
| B | HOH1427 |
| B | HOH1527 |
| B | HOH1645 |
| site_id | AD1 |
| Number of Residues | 6 |
| Details | binding site for residue MLI B 902 |
| Chain | Residue |
| B | ARG572 |
| B | LEU689 |
| B | HOH1027 |
| B | HOH1147 |
| B | HOH1250 |
| B | HOH1617 |
| site_id | AD2 |
| Number of Residues | 7 |
| Details | binding site for residue MLI B 903 |
| Chain | Residue |
| B | ARG405 |
| B | ARG497 |
| B | HOH1032 |
| B | HOH1065 |
| B | HOH1088 |
| B | HOH1112 |
| B | HOH1638 |
| site_id | AD3 |
| Number of Residues | 5 |
| Details | binding site for residue MLI B 904 |
| Chain | Residue |
| B | HIS519 |
| B | ASP535 |
| B | LEU536 |
| B | SER537 |
| B | HOH1010 |
| site_id | AD4 |
| Number of Residues | 2 |
| Details | binding site for residue MLI B 905 |
| Chain | Residue |
| B | HOH1020 |
| B | HOH1652 |
| site_id | AD5 |
| Number of Residues | 9 |
| Details | binding site for residue CHT B 906 |
| Chain | Residue |
| B | PHE208 |
| B | ASP216 |
| B | GLY488 |
| B | CYS489 |
| B | GLU491 |
| B | THR502 |
| B | TYR506 |
| B | LEU698 |
| B | ILE700 |
Functional Information from PROSITE/UniProt
| site_id | PS00850 |
| Number of Residues | 9 |
| Details | GLY_RADICAL_1 Glycine radical domain signature. VvRVAGYSA |
| Chain | Residue | Details |
| A | VAL816-ALA824 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 658 |
| Details | Domain: {"description":"PFL","evidences":[{"source":"PROSITE-ProRule","id":"PRU00887","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 242 |
| Details | Domain: {"description":"Glycine radical","evidences":[{"source":"PROSITE-ProRule","id":"PRU00493","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 2 |
| Details | Active site: {"description":"Cysteine radical intermediate","evidences":[{"source":"HAMAP-Rule","id":"MF_02058","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"23151509","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"24854437","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 2 |
| Details | Active site: {"description":"Proton acceptor","evidences":[{"source":"HAMAP-Rule","id":"MF_02058","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"24854437","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 2 |
| Details | Modified residue: {"description":"Glycine radical","evidences":[{"source":"HAMAP-Rule","id":"MF_02058","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"23151509","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"24854437","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
