5FAV
E491Q mutant of choline TMA-lyase
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003824 | molecular_function | catalytic activity |
| A | 0005829 | cellular_component | cytosol |
| A | 0016829 | molecular_function | lyase activity |
| A | 0016840 | molecular_function | carbon-nitrogen lyase activity |
| A | 0033265 | molecular_function | choline binding |
| A | 0042426 | biological_process | choline catabolic process |
| A | 0042803 | molecular_function | protein homodimerization activity |
| A | 0120525 | molecular_function | choline trimethylamine lyase activity |
| B | 0003824 | molecular_function | catalytic activity |
| B | 0005829 | cellular_component | cytosol |
| B | 0016829 | molecular_function | lyase activity |
| B | 0016840 | molecular_function | carbon-nitrogen lyase activity |
| B | 0033265 | molecular_function | choline binding |
| B | 0042426 | biological_process | choline catabolic process |
| B | 0042803 | molecular_function | protein homodimerization activity |
| B | 0120525 | molecular_function | choline trimethylamine lyase activity |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 6 |
| Details | binding site for residue NA A 901 |
| Chain | Residue |
| A | SER746 |
| A | MET748 |
| A | HOH1321 |
| A | HOH1552 |
| A | HOH1750 |
| B | HOH1731 |
| site_id | AC2 |
| Number of Residues | 7 |
| Details | binding site for residue MLI A 902 |
| Chain | Residue |
| A | HOH1049 |
| A | HOH1205 |
| A | HOH1565 |
| A | HOH1619 |
| A | ARG572 |
| A | LEU689 |
| A | HOH1009 |
| site_id | AC3 |
| Number of Residues | 6 |
| Details | binding site for residue MLI A 903 |
| Chain | Residue |
| A | ARG238 |
| A | THR633 |
| A | GLN636 |
| A | HOH1182 |
| A | HOH1186 |
| A | HOH1421 |
| site_id | AC4 |
| Number of Residues | 6 |
| Details | binding site for residue MLI A 904 |
| Chain | Residue |
| A | LYS102 |
| A | ARG105 |
| A | TYR106 |
| A | HOH1074 |
| A | HOH1342 |
| A | HOH1713 |
| site_id | AC5 |
| Number of Residues | 9 |
| Details | binding site for residue MLI A 905 |
| Chain | Residue |
| A | GLY49 |
| A | SER50 |
| A | HIS51 |
| A | HOH1030 |
| A | HOH1048 |
| A | HOH1058 |
| A | HOH1091 |
| A | HOH1187 |
| B | LYS71 |
| site_id | AC6 |
| Number of Residues | 7 |
| Details | binding site for residue MLI A 906 |
| Chain | Residue |
| A | HIS519 |
| A | ASP535 |
| A | LEU536 |
| A | HOH1008 |
| A | HOH1038 |
| A | HOH1051 |
| A | HOH1121 |
| site_id | AC7 |
| Number of Residues | 6 |
| Details | binding site for residue NA A 907 |
| Chain | Residue |
| A | GLU242 |
| A | LEU244 |
| A | HOH1011 |
| A | HOH1110 |
| A | HOH1614 |
| A | HOH1721 |
| site_id | AC8 |
| Number of Residues | 6 |
| Details | binding site for residue NA B 901 |
| Chain | Residue |
| A | HOH1719 |
| B | SER746 |
| B | MET748 |
| B | HOH1448 |
| B | HOH1587 |
| B | HOH1668 |
| site_id | AC9 |
| Number of Residues | 8 |
| Details | binding site for residue MLI B 902 |
| Chain | Residue |
| B | ARG572 |
| B | LEU689 |
| B | HOH1015 |
| B | HOH1072 |
| B | HOH1170 |
| B | HOH1268 |
| B | HOH1504 |
| B | HOH1660 |
| site_id | AD1 |
| Number of Residues | 7 |
| Details | binding site for residue MLI B 903 |
| Chain | Residue |
| B | ARG405 |
| B | ARG497 |
| B | HOH1040 |
| B | HOH1047 |
| B | HOH1057 |
| B | HOH1110 |
| B | HOH1688 |
| site_id | AD2 |
| Number of Residues | 5 |
| Details | binding site for residue MLI B 904 |
| Chain | Residue |
| B | HIS519 |
| B | ASP535 |
| B | LEU536 |
| B | SER537 |
| B | HOH1004 |
| site_id | AD3 |
| Number of Residues | 6 |
| Details | binding site for residue MLI B 905 |
| Chain | Residue |
| B | SER50 |
| B | HIS51 |
| B | MET52 |
| B | GLY53 |
| B | HOH1081 |
| B | HOH1408 |
Functional Information from PROSITE/UniProt
| site_id | PS00850 |
| Number of Residues | 9 |
| Details | GLY_RADICAL_1 Glycine radical domain signature. VvRVAGYSA |
| Chain | Residue | Details |
| A | VAL816-ALA824 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 1316 |
| Details | Domain: {"description":"PFL","evidences":[{"source":"PROSITE-ProRule","id":"PRU00887","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 242 |
| Details | Domain: {"description":"Glycine radical","evidences":[{"source":"PROSITE-ProRule","id":"PRU00493","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 2 |
| Details | Active site: {"description":"Cysteine radical intermediate","evidences":[{"source":"HAMAP-Rule","id":"MF_02058","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"23151509","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"24854437","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 2 |
| Details | Active site: {"description":"Proton acceptor","evidences":[{"source":"HAMAP-Rule","id":"MF_02058","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"24854437","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 2 |
| Details | Modified residue: {"description":"Glycine radical","evidences":[{"source":"HAMAP-Rule","id":"MF_02058","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"23151509","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"24854437","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
