5EPU
X-ray structure uridine phosphorylase from Vibrio cholerae in complex with cytosine at 1.06A.
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003824 | molecular_function | catalytic activity |
| A | 0004731 | molecular_function | purine-nucleoside phosphorylase activity |
| A | 0004850 | molecular_function | uridine phosphorylase activity |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005829 | cellular_component | cytosol |
| A | 0006152 | biological_process | purine nucleoside catabolic process |
| A | 0006218 | biological_process | uridine catabolic process |
| A | 0009116 | biological_process | nucleoside metabolic process |
| A | 0009164 | biological_process | nucleoside catabolic process |
| A | 0009166 | biological_process | nucleotide catabolic process |
| A | 0016757 | molecular_function | glycosyltransferase activity |
| A | 0016763 | molecular_function | pentosyltransferase activity |
| A | 0044206 | biological_process | UMP salvage |
| A | 0046872 | molecular_function | metal ion binding |
| B | 0003824 | molecular_function | catalytic activity |
| B | 0004731 | molecular_function | purine-nucleoside phosphorylase activity |
| B | 0004850 | molecular_function | uridine phosphorylase activity |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005829 | cellular_component | cytosol |
| B | 0006152 | biological_process | purine nucleoside catabolic process |
| B | 0006218 | biological_process | uridine catabolic process |
| B | 0009116 | biological_process | nucleoside metabolic process |
| B | 0009164 | biological_process | nucleoside catabolic process |
| B | 0009166 | biological_process | nucleotide catabolic process |
| B | 0016757 | molecular_function | glycosyltransferase activity |
| B | 0016763 | molecular_function | pentosyltransferase activity |
| B | 0044206 | biological_process | UMP salvage |
| B | 0046872 | molecular_function | metal ion binding |
| C | 0003824 | molecular_function | catalytic activity |
| C | 0004731 | molecular_function | purine-nucleoside phosphorylase activity |
| C | 0004850 | molecular_function | uridine phosphorylase activity |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0005829 | cellular_component | cytosol |
| C | 0006152 | biological_process | purine nucleoside catabolic process |
| C | 0006218 | biological_process | uridine catabolic process |
| C | 0009116 | biological_process | nucleoside metabolic process |
| C | 0009164 | biological_process | nucleoside catabolic process |
| C | 0009166 | biological_process | nucleotide catabolic process |
| C | 0016757 | molecular_function | glycosyltransferase activity |
| C | 0016763 | molecular_function | pentosyltransferase activity |
| C | 0044206 | biological_process | UMP salvage |
| C | 0046872 | molecular_function | metal ion binding |
| D | 0003824 | molecular_function | catalytic activity |
| D | 0004731 | molecular_function | purine-nucleoside phosphorylase activity |
| D | 0004850 | molecular_function | uridine phosphorylase activity |
| D | 0005737 | cellular_component | cytoplasm |
| D | 0005829 | cellular_component | cytosol |
| D | 0006152 | biological_process | purine nucleoside catabolic process |
| D | 0006218 | biological_process | uridine catabolic process |
| D | 0009116 | biological_process | nucleoside metabolic process |
| D | 0009164 | biological_process | nucleoside catabolic process |
| D | 0009166 | biological_process | nucleotide catabolic process |
| D | 0016757 | molecular_function | glycosyltransferase activity |
| D | 0016763 | molecular_function | pentosyltransferase activity |
| D | 0044206 | biological_process | UMP salvage |
| D | 0046872 | molecular_function | metal ion binding |
| E | 0003824 | molecular_function | catalytic activity |
| E | 0004731 | molecular_function | purine-nucleoside phosphorylase activity |
| E | 0004850 | molecular_function | uridine phosphorylase activity |
| E | 0005737 | cellular_component | cytoplasm |
| E | 0005829 | cellular_component | cytosol |
| E | 0006152 | biological_process | purine nucleoside catabolic process |
| E | 0006218 | biological_process | uridine catabolic process |
| E | 0009116 | biological_process | nucleoside metabolic process |
| E | 0009164 | biological_process | nucleoside catabolic process |
| E | 0009166 | biological_process | nucleotide catabolic process |
| E | 0016757 | molecular_function | glycosyltransferase activity |
| E | 0016763 | molecular_function | pentosyltransferase activity |
| E | 0044206 | biological_process | UMP salvage |
| E | 0046872 | molecular_function | metal ion binding |
| F | 0003824 | molecular_function | catalytic activity |
| F | 0004731 | molecular_function | purine-nucleoside phosphorylase activity |
| F | 0004850 | molecular_function | uridine phosphorylase activity |
| F | 0005737 | cellular_component | cytoplasm |
| F | 0005829 | cellular_component | cytosol |
| F | 0006152 | biological_process | purine nucleoside catabolic process |
| F | 0006218 | biological_process | uridine catabolic process |
| F | 0009116 | biological_process | nucleoside metabolic process |
| F | 0009164 | biological_process | nucleoside catabolic process |
| F | 0009166 | biological_process | nucleotide catabolic process |
| F | 0016757 | molecular_function | glycosyltransferase activity |
| F | 0016763 | molecular_function | pentosyltransferase activity |
| F | 0044206 | biological_process | UMP salvage |
| F | 0046872 | molecular_function | metal ion binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 12 |
| Details | binding site for residue CYT A 301 |
| Chain | Residue |
| A | THR94 |
| A | HOH413 |
| A | HOH414 |
| A | HOH554 |
| A | GLY95 |
| A | GLN165 |
| A | ARG167 |
| A | GLU195 |
| A | MET196 |
| A | GOL303 |
| A | HOH403 |
| A | HOH409 |
| site_id | AC2 |
| Number of Residues | 6 |
| Details | binding site for residue GOL A 302 |
| Chain | Residue |
| A | ASP169 |
| A | HOH455 |
| A | HOH476 |
| A | HOH540 |
| A | HOH562 |
| A | HOH609 |
| site_id | AC3 |
| Number of Residues | 14 |
| Details | binding site for residue GOL A 303 |
| Chain | Residue |
| A | ILE68 |
| A | THR93 |
| A | PHE161 |
| A | GLU195 |
| A | MET196 |
| A | GLU197 |
| A | CYT301 |
| A | HOH413 |
| A | HOH416 |
| A | HOH533 |
| A | HOH578 |
| B | HIS7 |
| B | ARG47 |
| B | HOH478 |
| site_id | AC4 |
| Number of Residues | 5 |
| Details | binding site for residue MG A 304 |
| Chain | Residue |
| A | HOH436 |
| A | HOH560 |
| A | HOH645 |
| A | HOH716 |
| A | HOH738 |
| site_id | AC5 |
| Number of Residues | 6 |
| Details | binding site for residue MG A 305 |
| Chain | Residue |
| A | HOH457 |
| A | HOH515 |
| A | HOH688 |
| A | HOH742 |
| A | HOH753 |
| B | HOH722 |
| site_id | AC6 |
| Number of Residues | 6 |
| Details | binding site for residue NA A 306 |
| Chain | Residue |
| A | GLU48 |
| A | ILE68 |
| A | SER72 |
| B | GLU48 |
| B | ILE68 |
| B | SER72 |
| site_id | AC7 |
| Number of Residues | 14 |
| Details | binding site for residue GOL B 301 |
| Chain | Residue |
| A | HIS7 |
| A | ARG47 |
| A | ILE75 |
| B | ILE68 |
| B | THR93 |
| B | PHE161 |
| B | MET196 |
| B | GLU197 |
| B | CYT304 |
| B | HOH421 |
| B | HOH422 |
| B | HOH434 |
| B | HOH500 |
| B | HOH613 |
| site_id | AC8 |
| Number of Residues | 2 |
| Details | binding site for residue CL B 302 |
| Chain | Residue |
| B | ASN221 |
| B | LYS225 |
| site_id | AC9 |
| Number of Residues | 5 |
| Details | binding site for residue MG B 303 |
| Chain | Residue |
| B | HOH426 |
| B | HOH648 |
| F | HOH414 |
| F | HOH566 |
| F | HOH765 |
| site_id | AD1 |
| Number of Residues | 11 |
| Details | binding site for residue CYT B 304 |
| Chain | Residue |
| B | THR93 |
| B | THR94 |
| B | GLY95 |
| B | PHE161 |
| B | GLN165 |
| B | ARG167 |
| B | GLU195 |
| B | MET196 |
| B | ILE220 |
| B | GOL301 |
| B | HOH503 |
| site_id | AD2 |
| Number of Residues | 15 |
| Details | binding site for residue TRS B 305 |
| Chain | Residue |
| B | ARG178 |
| B | SER182 |
| B | GLU185 |
| B | TRP186 |
| B | HOH402 |
| B | HOH404 |
| B | HOH406 |
| B | HOH409 |
| B | HOH512 |
| B | HOH564 |
| B | HOH668 |
| C | PRO124 |
| C | GLU126 |
| C | HOH402 |
| D | ARG177 |
| site_id | AD3 |
| Number of Residues | 2 |
| Details | binding site for residue CL C 301 |
| Chain | Residue |
| C | ASN221 |
| C | LYS225 |
| site_id | AD4 |
| Number of Residues | 11 |
| Details | binding site for residue CYT C 302 |
| Chain | Residue |
| C | THR94 |
| C | GLY95 |
| C | PHE161 |
| C | GLN165 |
| C | ARG167 |
| C | GLU195 |
| C | MET196 |
| C | ILE220 |
| C | EDO303 |
| C | HOH490 |
| C | THR93 |
| site_id | AD5 |
| Number of Residues | 10 |
| Details | binding site for residue EDO C 303 |
| Chain | Residue |
| C | ILE68 |
| C | THR93 |
| C | GLU197 |
| C | CYT302 |
| C | HOH415 |
| C | HOH418 |
| C | HOH521 |
| C | HOH634 |
| D | HIS7 |
| D | ARG47 |
| site_id | AD6 |
| Number of Residues | 6 |
| Details | binding site for residue NA C 304 |
| Chain | Residue |
| C | GLU48 |
| C | ILE68 |
| C | SER72 |
| D | GLU48 |
| D | ILE68 |
| D | SER72 |
| site_id | AD7 |
| Number of Residues | 10 |
| Details | binding site for residue GOL D 301 |
| Chain | Residue |
| C | HIS7 |
| C | ARG47 |
| D | ILE68 |
| D | THR93 |
| D | MET196 |
| D | GLU197 |
| D | CYT302 |
| D | HOH401 |
| D | HOH423 |
| D | HOH461 |
| site_id | AD8 |
| Number of Residues | 14 |
| Details | binding site for residue CYT D 302 |
| Chain | Residue |
| D | THR93 |
| D | THR94 |
| D | GLY95 |
| D | PHE161 |
| D | GLN165 |
| D | ARG167 |
| D | GLU195 |
| D | MET196 |
| D | GOL301 |
| D | HOH403 |
| D | HOH409 |
| D | HOH412 |
| D | HOH414 |
| D | HOH523 |
| site_id | AD9 |
| Number of Residues | 6 |
| Details | binding site for residue MG E 301 |
| Chain | Residue |
| B | HOH446 |
| B | HOH550 |
| B | HOH683 |
| B | HOH688 |
| B | HOH721 |
| B | HOH724 |
| site_id | AE1 |
| Number of Residues | 11 |
| Details | binding site for residue GOL E 302 |
| Chain | Residue |
| E | ILE68 |
| E | THR93 |
| E | GLU195 |
| E | MET196 |
| E | GLU197 |
| E | CYT304 |
| E | HOH418 |
| E | HOH421 |
| E | HOH451 |
| F | HIS7 |
| F | ARG47 |
| site_id | AE2 |
| Number of Residues | 4 |
| Details | binding site for residue MG E 303 |
| Chain | Residue |
| E | ASN102 |
| E | HOH414 |
| E | HOH624 |
| E | HOH703 |
| site_id | AE3 |
| Number of Residues | 10 |
| Details | binding site for residue CYT E 304 |
| Chain | Residue |
| E | THR94 |
| E | GLY95 |
| E | PHE161 |
| E | GLN165 |
| E | ARG167 |
| E | GLU195 |
| E | MET196 |
| E | ILE220 |
| E | GOL302 |
| E | HOH532 |
| site_id | AE4 |
| Number of Residues | 6 |
| Details | binding site for residue EDO E 305 |
| Chain | Residue |
| E | ARG167 |
| E | TYR168 |
| E | ASP169 |
| E | HOH430 |
| E | HOH449 |
| E | HOH600 |
| site_id | AE5 |
| Number of Residues | 6 |
| Details | binding site for residue NA E 306 |
| Chain | Residue |
| E | GLU48 |
| E | ILE68 |
| E | SER72 |
| F | GLU48 |
| F | ILE68 |
| F | SER72 |
| site_id | AE6 |
| Number of Residues | 11 |
| Details | binding site for residue GOL F 301 |
| Chain | Residue |
| E | HIS7 |
| E | ARG47 |
| F | ILE68 |
| F | THR93 |
| F | PHE161 |
| F | GLU197 |
| F | CYT304 |
| F | HOH413 |
| F | HOH423 |
| F | HOH494 |
| F | HOH610 |
| site_id | AE7 |
| Number of Residues | 7 |
| Details | binding site for residue MG F 302 |
| Chain | Residue |
| F | MG303 |
| F | HOH575 |
| F | HOH692 |
| F | HOH706 |
| F | HOH746 |
| F | HOH775 |
| F | HOH789 |
| site_id | AE8 |
| Number of Residues | 8 |
| Details | binding site for residue MG F 303 |
| Chain | Residue |
| F | ASN102 |
| F | MG302 |
| F | HOH418 |
| F | HOH575 |
| F | HOH647 |
| F | HOH692 |
| F | HOH702 |
| F | HOH746 |
| site_id | AE9 |
| Number of Residues | 10 |
| Details | binding site for residue CYT F 304 |
| Chain | Residue |
| F | THR94 |
| F | GLY95 |
| F | PHE161 |
| F | GLN165 |
| F | ARG167 |
| F | GLU195 |
| F | MET196 |
| F | ILE220 |
| F | GOL301 |
| F | HOH470 |
| site_id | AF1 |
| Number of Residues | 6 |
| Details | binding site for residue EDO F 305 |
| Chain | Residue |
| F | ARG167 |
| F | TYR168 |
| F | ASP169 |
| F | HOH435 |
| F | HOH454 |
| F | HOH611 |
| site_id | AF2 |
| Number of Residues | 14 |
| Details | binding site for residue TRS F 306 |
| Chain | Residue |
| A | PRO124 |
| A | GLU126 |
| A | HOH613 |
| B | ARG177 |
| F | ARG178 |
| F | GLU185 |
| F | HOH401 |
| F | HOH404 |
| F | HOH408 |
| F | HOH409 |
| F | HOH425 |
| F | HOH517 |
| F | HOH520 |
| F | HOH630 |
Functional Information from PROSITE/UniProt
| site_id | PS01232 |
| Number of Residues | 16 |
| Details | PNP_UDP_1 Purine and other phosphorylases family 1 signature. StGIGgPStSIaveEL |
| Chain | Residue | Details |
| A | SER65-LEU80 |
