5EKX

DENGUE 3 NS5 METHYLTRANSFERASE BOUND TO S-ADENOSYLMETHIONINE AND FRAGMENT NB2E11

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004482	molecular_function	mRNA 5'-cap (guanine-N7-)-methyltransferase activity
A	0004483	molecular_function	mRNA (nucleoside-2'-O-)-methyltransferase activity
A	0008168	molecular_function	methyltransferase activity
A	0032259	biological_process	methylation
B	0004482	molecular_function	mRNA 5'-cap (guanine-N7-)-methyltransferase activity
B	0004483	molecular_function	mRNA (nucleoside-2'-O-)-methyltransferase activity
B	0008168	molecular_function	methyltransferase activity
B	0032259	biological_process	methylation

Functional Information from PDB Data

site_id	AC1
Number of Residues	19
Details	binding site for residue SAM A 301

Chain	Residue
A	SER56
A	HIS110
A	LYS130
A	ASP131
A	VAL132
A	PHE133
A	ASP146
A	HOH404
A	HOH415
A	HOH437
A	HOH447
A	GLY58
A	GLY81
A	CYS82
A	GLY83
A	GLY86
A	TRP87
A	THR104
A	LYS105

---

site_id	AC2
Number of Residues	5
Details	binding site for residue 2CQ A 302

Chain	Residue
A	GLY107
A	PRO108
A	HIS110
A	GLU112
A	VAL234

---

site_id	AC3
Number of Residues	19
Details	binding site for residue SAM B 4000

Chain	Residue
B	SER56
B	GLY58
B	GLY81
B	CYS82
B	GLY86
B	TRP87
B	THR104
B	LYS105
B	HIS110
B	LYS130
B	ASP131
B	VAL132
B	PHE133
B	ASP146
B	ILE147
B	HOH4106
B	HOH4116
B	HOH4120
B	HOH4139

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	8
Details	ACT_SITE: For 2'-O-MTase activity => ECO:0000250|UniProtKB:Q6YMS4

Chain	Residue	Details
A	LYS61
A	ASP146
A	LYS180
A	GLU216
B	LYS61
B	ASP146
B	LYS180
B	GLU216

---

site_id	SWS_FT_FI2
Number of Residues	18
Details	BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00924

Chain	Residue	Details
A	SER56
B	SER56
B	GLY86
B	TRP87
B	THR104
B	LYS105
B	ASP131
B	VAL132
B	ILE147
B	TYR218
A	GLY86
A	TRP87
A	THR104
A	LYS105
A	ASP131
A	VAL132
A	ILE147
A	TYR218

---

site_id	SWS_FT_FI3
Number of Residues	14
Details	SITE: mRNA cap binding => ECO:0000255|PROSITE-ProRule:PRU00924

Chain	Residue	Details
A	LYS14
B	PHE25
B	LYS29
B	SER150
B	ARG211
B	SER213
A	ASN18
A	PHE25
A	LYS29
A	SER150
A	ARG211
A	SER213
B	LYS14
B	ASN18

---

site_id	SWS_FT_FI4
Number of Residues	4
Details	SITE: mRNA cap binding; via carbonyl oxygen => ECO:0000255|PROSITE-ProRule:PRU00924

Chain	Residue	Details
A	LEU17
A	LEU20
B	LEU17
B	LEU20

---

site_id	SWS_FT_FI5
Number of Residues	6
Details	SITE: Essential for 2'-O-methyltransferase activity => ECO:0000255|PROSITE-ProRule:PRU00924

Chain	Residue	Details
A	LYS61
A	LYS180
A	GLU216
B	LYS61
B	LYS180
B	GLU216

---

site_id	SWS_FT_FI6
Number of Residues	2
Details	SITE: Essential for 2'-O-methyltransferase and N-7 methyltransferase activity => ECO:0000255|PROSITE-ProRule:PRU00924

Chain	Residue	Details
A	ASP146
B	ASP146

---

site_id	SWS_FT_FI7
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:P03314

Chain	Residue	Details
A	SER56
B	SER56

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