5EIB

Crystal structure of CPAP PN2-3 C-terminal loop-helix in complex with DARPin-tubulin

Functional Information from GO Data

Chain	GOid	namespace	contents
C	0000226	biological_process	microtubule cytoskeleton organization
C	0000278	biological_process	mitotic cell cycle
C	0003924	molecular_function	GTPase activity
C	0005200	molecular_function	structural constituent of cytoskeleton
C	0005525	molecular_function	GTP binding
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0005856	cellular_component	cytoskeleton
C	0005874	cellular_component	microtubule
C	0007017	biological_process	microtubule-based process
C	0015630	cellular_component	microtubule cytoskeleton
C	0016787	molecular_function	hydrolase activity
C	0046872	molecular_function	metal ion binding
D	0000226	biological_process	microtubule cytoskeleton organization
D	0000278	biological_process	mitotic cell cycle
D	0003924	molecular_function	GTPase activity
D	0005200	molecular_function	structural constituent of cytoskeleton
D	0005525	molecular_function	GTP binding
D	0005737	cellular_component	cytoplasm
D	0005856	cellular_component	cytoskeleton
D	0005874	cellular_component	microtubule
D	0007017	biological_process	microtubule-based process
D	0007399	biological_process	nervous system development
D	0015630	cellular_component	microtubule cytoskeleton
D	0046872	molecular_function	metal ion binding
D	0046982	molecular_function	protein heterodimerization activity
D	1902669	biological_process	positive regulation of axon guidance

Functional Information from PDB Data

site_id	AC1
Number of Residues	27
Details	binding site for residue GTP C 501

Chain	Residue
C	GLY10
C	GLY144
C	THR145
C	GLY146
C	VAL177
C	GLU183
C	ASN206
C	TYR224
C	ASN228
C	ILE231
C	MG502
C	GLN11
C	HOH654
C	HOH661
C	HOH687
C	HOH709
C	HOH733
C	HOH750
C	HOH777
D	LYS254
C	ALA12
C	GLN15
C	ASP98
C	ALA99
C	ASN101
C	SER140
C	GLY143

---

site_id	AC2
Number of Residues	6
Details	binding site for residue MG C 502

Chain	Residue
C	GTP501
C	HOH661
C	HOH666
C	HOH733
C	HOH777
D	LYS254

---

site_id	AC3
Number of Residues	27
Details	binding site for residue GTP D 501

Chain	Residue
D	GLY10
D	GLN11
D	CYS12
D	GLN15
D	ALA99
D	GLY100
D	ASN101
D	SER140
D	GLY142
D	GLY143
D	GLY144
D	THR145
D	GLY146
D	VAL177
D	SER178
D	GLU183
D	ASN206
D	TYR224
D	ASN228
D	MG502
D	HOH615
D	HOH625
D	HOH626
D	HOH628
D	HOH640
D	HOH657
D	HOH683

---

site_id	AC4
Number of Residues	5
Details	binding site for residue MG D 502

Chain	Residue
D	GTP501
D	HOH610
D	HOH615
D	HOH625
D	HOH628

---

Functional Information from PROSITE/UniProt

site_id	PS00227
Number of Residues	7
Details	TUBULIN Tubulin subunits alpha, beta, and gamma signature. GGGTGSG

Chain	Residue	Details
D	GLY142-GLY148
C	GLY142-GLY148

---

site_id	PS00228
Number of Residues	4
Details	TUBULIN_B_AUTOREG Tubulin-beta mRNA autoregulation signal. MREI

Chain	Residue	Details
D	MET1-ILE4

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	7
Details	BINDING: BINDING => ECO:0000250|UniProtKB:Q13509

Chain	Residue	Details
D	GLN11
D	SER140
D	GLY144
D	THR145
D	GLY146
D	ASN206
D	ASN228

---

site_id	SWS_FT_FI2
Number of Residues	1
Details	BINDING: BINDING => ECO:0000250|UniProtKB:P68363

Chain	Residue	Details
D	GLU71
C	GLU71
C	SER140
C	GLY144
C	THR145
C	THR179
C	ASN206
C	ASN228

---

site_id	SWS_FT_FI3
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:P99024

Chain	Residue	Details
D	SER40

---

site_id	SWS_FT_FI4
Number of Residues	1
Details	MOD_RES: Phosphothreonine => ECO:0000250|UniProtKB:Q3KRE8

Chain	Residue	Details
D	THR57

---

site_id	SWS_FT_FI5
Number of Residues	1
Details	MOD_RES: N6-succinyllysine; alternate => ECO:0000250|UniProtKB:P99024

Chain	Residue	Details
D	LYS60
C	SER232

---

site_id	SWS_FT_FI6
Number of Residues	1
Details	MOD_RES: Phosphoserine; by CDK1 => ECO:0000250|UniProtKB:Q9BVA1

Chain	Residue	Details
D	SER174

---

site_id	SWS_FT_FI7
Number of Residues	2
Details	MOD_RES: Phosphothreonine => ECO:0000250|UniProtKB:P07437

Chain	Residue	Details
D	THR287
D	THR292

---

site_id	SWS_FT_FI8
Number of Residues	1
Details	MOD_RES: Omega-N-methylarginine => ECO:0000250|UniProtKB:P07437

Chain	Residue	Details
D	ARG320

---

site_id	SWS_FT_FI9
Number of Residues	1
Details	MOD_RES: 5-glutamyl polyglutamate => ECO:0000250|UniProtKB:Q2T9S0

Chain	Residue	Details
D	GLU448

---

site_id	SWS_FT_FI10
Number of Residues	1
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternate => ECO:0000250|UniProtKB:P07437

Chain	Residue	Details
D	LYS60

---

site_id	SWS_FT_FI11
Number of Residues	2
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000250|UniProtKB:P07437

Chain	Residue	Details
C	TYR451
D	LYS326

---

site_id	SWS_FT_FI12
Number of Residues	3
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000250|UniProtKB:P68363

Chain	Residue	Details
C	LYS326
C	LYS370

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