5EE4
The crystal structure of HpuA from Kingella denitrificans in complex with human haemoglobin
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0009279 | cellular_component | cell outer membrane |
| B | 0009279 | cellular_component | cell outer membrane |
| C | 0004601 | molecular_function | peroxidase activity |
| C | 0005344 | molecular_function | oxygen carrier activity |
| C | 0005506 | molecular_function | iron ion binding |
| C | 0005515 | molecular_function | protein binding |
| C | 0005576 | cellular_component | extracellular region |
| C | 0005615 | cellular_component | extracellular space |
| C | 0005829 | cellular_component | cytosol |
| C | 0005833 | cellular_component | hemoglobin complex |
| C | 0006954 | biological_process | inflammatory response |
| C | 0015670 | biological_process | carbon dioxide transport |
| C | 0015671 | biological_process | oxygen transport |
| C | 0016020 | cellular_component | membrane |
| C | 0019825 | molecular_function | oxygen binding |
| C | 0020037 | molecular_function | heme binding |
| C | 0030185 | biological_process | nitric oxide transport |
| C | 0031720 | molecular_function | haptoglobin binding |
| C | 0031838 | cellular_component | haptoglobin-hemoglobin complex |
| C | 0042542 | biological_process | response to hydrogen peroxide |
| C | 0042744 | biological_process | hydrogen peroxide catabolic process |
| C | 0046872 | molecular_function | metal ion binding |
| C | 0048821 | biological_process | erythrocyte development |
| C | 0070062 | cellular_component | extracellular exosome |
| C | 0071682 | cellular_component | endocytic vesicle lumen |
| C | 0072562 | cellular_component | blood microparticle |
| C | 0098869 | biological_process | cellular oxidant detoxification |
| D | 0004601 | molecular_function | peroxidase activity |
| D | 0005344 | molecular_function | oxygen carrier activity |
| D | 0005515 | molecular_function | protein binding |
| D | 0005576 | cellular_component | extracellular region |
| D | 0005615 | cellular_component | extracellular space |
| D | 0005829 | cellular_component | cytosol |
| D | 0005833 | cellular_component | hemoglobin complex |
| D | 0006954 | biological_process | inflammatory response |
| D | 0008217 | biological_process | regulation of blood pressure |
| D | 0015670 | biological_process | carbon dioxide transport |
| D | 0015671 | biological_process | oxygen transport |
| D | 0019825 | molecular_function | oxygen binding |
| D | 0020037 | molecular_function | heme binding |
| D | 0030185 | biological_process | nitric oxide transport |
| D | 0030492 | molecular_function | hemoglobin binding |
| D | 0031720 | molecular_function | haptoglobin binding |
| D | 0031721 | molecular_function | hemoglobin alpha binding |
| D | 0031838 | cellular_component | haptoglobin-hemoglobin complex |
| D | 0042542 | biological_process | response to hydrogen peroxide |
| D | 0042744 | biological_process | hydrogen peroxide catabolic process |
| D | 0045429 | biological_process | positive regulation of nitric oxide biosynthetic process |
| D | 0046872 | molecular_function | metal ion binding |
| D | 0048821 | biological_process | erythrocyte development |
| D | 0070062 | cellular_component | extracellular exosome |
| D | 0070293 | biological_process | renal absorption |
| D | 0070527 | biological_process | platelet aggregation |
| D | 0071682 | cellular_component | endocytic vesicle lumen |
| D | 0072562 | cellular_component | blood microparticle |
| D | 0097746 | biological_process | blood vessel diameter maintenance |
| D | 0098869 | biological_process | cellular oxidant detoxification |
| D | 1904724 | cellular_component | tertiary granule lumen |
| D | 1904813 | cellular_component | ficolin-1-rich granule lumen |
| E | 0004601 | molecular_function | peroxidase activity |
| E | 0005344 | molecular_function | oxygen carrier activity |
| E | 0005506 | molecular_function | iron ion binding |
| E | 0005515 | molecular_function | protein binding |
| E | 0005576 | cellular_component | extracellular region |
| E | 0005615 | cellular_component | extracellular space |
| E | 0005829 | cellular_component | cytosol |
| E | 0005833 | cellular_component | hemoglobin complex |
| E | 0006954 | biological_process | inflammatory response |
| E | 0015670 | biological_process | carbon dioxide transport |
| E | 0015671 | biological_process | oxygen transport |
| E | 0016020 | cellular_component | membrane |
| E | 0019825 | molecular_function | oxygen binding |
| E | 0020037 | molecular_function | heme binding |
| E | 0030185 | biological_process | nitric oxide transport |
| E | 0031720 | molecular_function | haptoglobin binding |
| E | 0031838 | cellular_component | haptoglobin-hemoglobin complex |
| E | 0042542 | biological_process | response to hydrogen peroxide |
| E | 0042744 | biological_process | hydrogen peroxide catabolic process |
| E | 0046872 | molecular_function | metal ion binding |
| E | 0048821 | biological_process | erythrocyte development |
| E | 0070062 | cellular_component | extracellular exosome |
| E | 0071682 | cellular_component | endocytic vesicle lumen |
| E | 0072562 | cellular_component | blood microparticle |
| E | 0098869 | biological_process | cellular oxidant detoxification |
| F | 0004601 | molecular_function | peroxidase activity |
| F | 0005344 | molecular_function | oxygen carrier activity |
| F | 0005515 | molecular_function | protein binding |
| F | 0005576 | cellular_component | extracellular region |
| F | 0005615 | cellular_component | extracellular space |
| F | 0005829 | cellular_component | cytosol |
| F | 0005833 | cellular_component | hemoglobin complex |
| F | 0006954 | biological_process | inflammatory response |
| F | 0008217 | biological_process | regulation of blood pressure |
| F | 0015670 | biological_process | carbon dioxide transport |
| F | 0015671 | biological_process | oxygen transport |
| F | 0019825 | molecular_function | oxygen binding |
| F | 0020037 | molecular_function | heme binding |
| F | 0030185 | biological_process | nitric oxide transport |
| F | 0030492 | molecular_function | hemoglobin binding |
| F | 0031720 | molecular_function | haptoglobin binding |
| F | 0031721 | molecular_function | hemoglobin alpha binding |
| F | 0031838 | cellular_component | haptoglobin-hemoglobin complex |
| F | 0042542 | biological_process | response to hydrogen peroxide |
| F | 0042744 | biological_process | hydrogen peroxide catabolic process |
| F | 0045429 | biological_process | positive regulation of nitric oxide biosynthetic process |
| F | 0046872 | molecular_function | metal ion binding |
| F | 0048821 | biological_process | erythrocyte development |
| F | 0070062 | cellular_component | extracellular exosome |
| F | 0070293 | biological_process | renal absorption |
| F | 0070527 | biological_process | platelet aggregation |
| F | 0071682 | cellular_component | endocytic vesicle lumen |
| F | 0072562 | cellular_component | blood microparticle |
| F | 0097746 | biological_process | blood vessel diameter maintenance |
| F | 0098869 | biological_process | cellular oxidant detoxification |
| F | 1904724 | cellular_component | tertiary granule lumen |
| F | 1904813 | cellular_component | ficolin-1-rich granule lumen |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 4 |
| Details | binding site for residue GOL A 401 |
| Chain | Residue |
| A | GLU269 |
| A | HIS278 |
| A | GLY282 |
| A | ARG304 |
| site_id | AC2 |
| Number of Residues | 13 |
| Details | binding site for residue HEM C 201 |
| Chain | Residue |
| C | LEU86 |
| C | HIS87 |
| C | LEU91 |
| C | ASN97 |
| C | PHE98 |
| C | OXY202 |
| C | HOH306 |
| C | HOH327 |
| C | TYR42 |
| C | PHE43 |
| C | PHE46 |
| C | HIS58 |
| C | LEU83 |
| site_id | AC3 |
| Number of Residues | 3 |
| Details | binding site for residue OXY C 202 |
| Chain | Residue |
| C | HIS58 |
| C | VAL62 |
| C | HEM201 |
| site_id | AC4 |
| Number of Residues | 11 |
| Details | binding site for residue HEM D 201 |
| Chain | Residue |
| D | PHE41 |
| D | PHE42 |
| D | HIS63 |
| D | LEU88 |
| D | HIS92 |
| D | LEU96 |
| D | ASN102 |
| D | LEU106 |
| D | VAL137 |
| D | LEU141 |
| D | OXY202 |
| site_id | AC5 |
| Number of Residues | 3 |
| Details | binding site for residue OXY D 202 |
| Chain | Residue |
| D | HIS63 |
| D | VAL67 |
| D | HEM201 |
| site_id | AC6 |
| Number of Residues | 6 |
| Details | binding site for residue GOL D 203 |
| Chain | Residue |
| D | VAL1 |
| D | LEU78 |
| D | ASP79 |
| D | ASN80 |
| D | LEU81 |
| D | LYS82 |
| site_id | AC7 |
| Number of Residues | 14 |
| Details | binding site for residue HEM E 201 |
| Chain | Residue |
| E | TYR42 |
| E | PHE43 |
| E | HIS58 |
| E | LYS61 |
| E | LEU83 |
| E | HIS87 |
| E | LEU91 |
| E | ASN97 |
| E | PHE98 |
| E | LEU101 |
| E | LEU136 |
| E | OXY202 |
| E | HOH320 |
| E | HOH339 |
| site_id | AC8 |
| Number of Residues | 3 |
| Details | binding site for residue OXY E 202 |
| Chain | Residue |
| E | HIS58 |
| E | VAL62 |
| E | HEM201 |
| site_id | AC9 |
| Number of Residues | 13 |
| Details | binding site for residue HEM F 201 |
| Chain | Residue |
| F | PHE41 |
| F | PHE42 |
| F | HIS63 |
| F | PHE71 |
| F | LEU88 |
| F | LEU91 |
| F | HIS92 |
| F | LEU96 |
| F | ASN102 |
| F | LEU106 |
| F | VAL137 |
| F | LEU141 |
| F | OXY202 |
| site_id | AD1 |
| Number of Residues | 3 |
| Details | binding site for residue OXY F 202 |
| Chain | Residue |
| F | HIS63 |
| F | VAL67 |
| F | HEM201 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 16 |
| Details | Peptide: {"description":"Hemopressin","featureId":"PRO_0000455882","evidences":[{"source":"PubMed","id":"18077343","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 424 |
| Details | Domain: {"description":"Globin","evidences":[{"source":"PROSITE-ProRule","id":"PRU00238","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 2 |
| Details | Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU00238","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 2 |
| Details | Binding site: {"description":"proximal binding residue","evidences":[{"source":"PROSITE-ProRule","id":"PRU00238","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 66 |
| Details | Site: {"description":"(Microbial infection) Cleavage; by N.americanus apr-2","evidences":[{"source":"PubMed","id":"12552433","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 16 |
| Details | Site: {"description":"Not glycated","evidences":[{"source":"PubMed","id":"7358733","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI7 |
| Number of Residues | 10 |
| Details | Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"24275569","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI8 |
| Number of Residues | 6 |
| Details | Modified residue: {"description":"N6-succinyllysine; alternate","evidences":[{"source":"UniProtKB","id":"P01942","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI9 |
| Number of Residues | 8 |
| Details | Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"PubMed","id":"24275569","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI10 |
| Number of Residues | 2 |
| Details | Modified residue: {"description":"N6-succinyllysine","evidences":[{"source":"UniProtKB","id":"P01942","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI11 |
| Number of Residues | 2 |
| Details | Modified residue: {"description":"Phosphotyrosine","evidences":[{"source":"PubMed","id":"24275569","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI12 |
| Number of Residues | 8 |
| Details | Modified residue: {"description":"Phosphoserine","evidences":[{"source":"UniProtKB","id":"P01942","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI13 |
| Number of Residues | 6 |
| Details | Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"UniProtKB","id":"P01942","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI14 |
| Number of Residues | 8 |
| Details | Glycosylation: {"description":"N-linked (Glc) (glycation) lysine; alternate","evidences":[{"source":"PubMed","id":"7358733","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI15 |
| Number of Residues | 10 |
| Details | Glycosylation: {"description":"N-linked (Glc) (glycation) lysine","evidences":[{"source":"PubMed","id":"7358733","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI16 |
| Number of Residues | 18 |
| Details | Peptide: {"description":"LVV-hemorphin-7","featureId":"PRO_0000296641"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI17 |
| Number of Residues | 12 |
| Details | Peptide: {"description":"Spinorphin","featureId":"PRO_0000424226"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI18 |
| Number of Residues | 6 |
| Details | Binding site: {} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI19 |
| Number of Residues | 2 |
| Details | Binding site: {"description":"distal binding residue"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI20 |
| Number of Residues | 2 |
| Details | Binding site: {"description":"proximal binding residue"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI21 |
| Number of Residues | 1 |
| Details | Modified residue: {"description":"N-pyruvate 2-iminyl-valine; in Hb A1b"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI22 |
| Number of Residues | 4 |
| Details | Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"PubMed","id":"4531009","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI23 |
| Number of Residues | 2 |
| Details | Modified residue: {"description":"S-nitrosocysteine","evidences":[{"source":"PubMed","id":"8637569","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"9843411","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI24 |
| Number of Residues | 2 |
| Details | Modified residue: {"description":"N6-acetyllysine; alternate","evidences":[{"source":"PubMed","id":"4531009","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI25 |
| Number of Residues | 1 |
| Details | Glycosylation: {"description":"N-linked (Glc) (glycation) valine; in Hb A1c","evidences":[{"source":"PubMed","id":"635569","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
