5EC8

DENGUE 3 NS5 METHYLTRANSFERASE BOUND TO S-ADENOSYL METHIONINE AND COMPOUND BF175

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004482	molecular_function	mRNA 5'-cap (guanine-N7-)-methyltransferase activity
A	0004483	molecular_function	mRNA (nucleoside-2'-O-)-methyltransferase activity
A	0008168	molecular_function	methyltransferase activity
A	0032259	biological_process	methylation
C	0004482	molecular_function	mRNA 5'-cap (guanine-N7-)-methyltransferase activity
C	0004483	molecular_function	mRNA (nucleoside-2'-O-)-methyltransferase activity
C	0008168	molecular_function	methyltransferase activity
C	0032259	biological_process	methylation

Functional Information from PDB Data

site_id	AC1
Number of Residues	20
Details	binding site for residue SAM A 301

Chain	Residue
A	SER56
A	HIS110
A	GLU111
A	LYS130
A	ASP131
A	VAL132
A	PHE133
A	ASP146
A	HOH410
A	HOH413
A	HOH423
A	GLY58
A	HOH588
A	GLY81
A	CYS82
A	GLY83
A	GLY86
A	TRP87
A	THR104
A	LYS105

---

site_id	AC2
Number of Residues	10
Details	binding site for residue 5LP A 302

Chain	Residue
A	ARG84
A	GLY107
A	PRO108
A	GLY109
A	HIS110
A	GLU112
A	MET233
A	HOH451
A	HOH507
A	HOH551

---

site_id	AC3
Number of Residues	20
Details	binding site for residue SAM C 4000

Chain	Residue
C	SER56
C	GLY58
C	GLY81
C	CYS82
C	GLY83
C	GLY86
C	TRP87
C	THR104
C	LYS105
C	HIS110
C	LYS130
C	ASP131
C	VAL132
C	PHE133
C	ASP146
C	ILE147
C	HOH4102
C	HOH4124
C	HOH4169
C	HOH4229

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	8
Details	ACT_SITE: For 2'-O-MTase activity => ECO:0000250|UniProtKB:Q6YMS4

Chain	Residue	Details
A	LYS61
A	ASP146
A	LYS180
A	GLU216
C	LYS61
C	ASP146
C	LYS180
C	GLU216

---

site_id	SWS_FT_FI2
Number of Residues	18
Details	BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00924

Chain	Residue	Details
A	SER56
C	SER56
C	GLY86
C	TRP87
C	THR104
C	LYS105
C	ASP131
C	VAL132
C	ILE147
C	TYR218
A	GLY86
A	TRP87
A	THR104
A	LYS105
A	ASP131
A	VAL132
A	ILE147
A	TYR218

---

site_id	SWS_FT_FI3
Number of Residues	14
Details	SITE: mRNA cap binding => ECO:0000255|PROSITE-ProRule:PRU00924

Chain	Residue	Details
A	LYS14
C	PHE25
C	LYS29
C	SER150
C	ARG211
C	SER213
A	ASN18
A	PHE25
A	LYS29
A	SER150
A	ARG211
A	SER213
C	LYS14
C	ASN18

---

site_id	SWS_FT_FI4
Number of Residues	4
Details	SITE: mRNA cap binding; via carbonyl oxygen => ECO:0000255|PROSITE-ProRule:PRU00924

Chain	Residue	Details
A	LEU17
A	LEU20
C	LEU17
C	LEU20

---

site_id	SWS_FT_FI5
Number of Residues	6
Details	SITE: Essential for 2'-O-methyltransferase activity => ECO:0000255|PROSITE-ProRule:PRU00924

Chain	Residue	Details
A	LYS61
A	LYS180
A	GLU216
C	LYS61
C	LYS180
C	GLU216

---

site_id	SWS_FT_FI6
Number of Residues	2
Details	SITE: Essential for 2'-O-methyltransferase and N-7 methyltransferase activity => ECO:0000255|PROSITE-ProRule:PRU00924

Chain	Residue	Details
A	ASP146
C	ASP146

---

site_id	SWS_FT_FI7
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:P03314

Chain	Residue	Details
A	SER56
C	SER56

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