5E9W
Crystal structure of mRNA cap guanine-N7 methyltransferase obtained by limited proteolysis
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004482 | molecular_function | mRNA 5'-cap (guanine-N7-)-methyltransferase activity |
| A | 0006370 | biological_process | 7-methylguanosine mRNA capping |
| B | 0004482 | molecular_function | mRNA 5'-cap (guanine-N7-)-methyltransferase activity |
| B | 0006370 | biological_process | 7-methylguanosine mRNA capping |
| C | 0004482 | molecular_function | mRNA 5'-cap (guanine-N7-)-methyltransferase activity |
| C | 0006370 | biological_process | 7-methylguanosine mRNA capping |
| D | 0004482 | molecular_function | mRNA 5'-cap (guanine-N7-)-methyltransferase activity |
| D | 0006370 | biological_process | 7-methylguanosine mRNA capping |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 16 |
| Details | binding site for residue SAH A 500 |
| Chain | Residue |
| A | LYS180 |
| A | PHE285 |
| A | VAL286 |
| A | TYR289 |
| A | HOH609 |
| A | HOH615 |
| A | HOH623 |
| A | HOH634 |
| A | GLY205 |
| A | CYS206 |
| A | GLY207 |
| A | ASP227 |
| A | ILE228 |
| A | ASP261 |
| A | SER262 |
| A | GLN284 |
| site_id | AC2 |
| Number of Residues | 19 |
| Details | binding site for residue SAH B 501 |
| Chain | Residue |
| B | LYS180 |
| B | GLY205 |
| B | CYS206 |
| B | GLY207 |
| B | ASP227 |
| B | ILE228 |
| B | ASP261 |
| B | SER262 |
| B | GLN284 |
| B | PHE285 |
| B | VAL286 |
| B | TYR289 |
| B | HOH607 |
| B | HOH623 |
| B | HOH634 |
| B | HOH635 |
| B | HOH640 |
| B | HOH646 |
| B | HOH655 |
| site_id | AC3 |
| Number of Residues | 16 |
| Details | binding site for residue SAH C 501 |
| Chain | Residue |
| C | LYS180 |
| C | GLY205 |
| C | CYS206 |
| C | GLY207 |
| C | ASP227 |
| C | ILE228 |
| C | ASP261 |
| C | SER262 |
| C | GLN284 |
| C | PHE285 |
| C | VAL286 |
| C | TYR289 |
| C | HOH612 |
| C | HOH613 |
| C | HOH627 |
| C | HOH629 |
| site_id | AC4 |
| Number of Residues | 16 |
| Details | binding site for residue SAH D 501 |
| Chain | Residue |
| D | LYS180 |
| D | GLY205 |
| D | GLY207 |
| D | ASP227 |
| D | ILE228 |
| D | ASP261 |
| D | SER262 |
| D | GLN284 |
| D | PHE285 |
| D | VAL286 |
| D | TYR289 |
| D | HOH616 |
| D | HOH619 |
| D | HOH644 |
| D | HOH647 |
| D | HOH662 |
| site_id | AC5 |
| Number of Residues | 26 |
| Details | binding site for Di-peptide ARG C 246 and GLN D 416 |
| Chain | Residue |
| B | ARG272 |
| C | PHE170 |
| C | ASN174 |
| C | ASP242 |
| C | LYS244 |
| C | ASN245 |
| C | ARG247 |
| C | LEU412 |
| C | LYS413 |
| C | ARG414 |
| C | MET415 |
| D | PHE170 |
| D | PHE170 |
| D | TYR171 |
| D | ASP242 |
| D | MET243 |
| D | LYS244 |
| D | ASN245 |
| D | ARG247 |
| D | ARG247 |
| D | ASP248 |
| D | SER249 |
| D | LEU412 |
| D | LYS413 |
| D | ARG414 |
| D | MET415 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 4 |
| Details | BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00895 |
| Chain | Residue | Details |
| A | ASN176 | |
| B | ASN176 | |
| C | ASN176 | |
| D | ASN176 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 12 |
| Details | BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00895, ECO:0000269|PubMed:27422871, ECO:0007744|PDB:3BGV, ECO:0007744|PDB:5E9J, ECO:0007744|PDB:5E9W |
| Chain | Residue | Details |
| A | LYS180 | |
| D | LYS180 | |
| D | GLY205 | |
| D | ASP227 | |
| A | GLY205 | |
| A | ASP227 | |
| B | LYS180 | |
| B | GLY205 | |
| B | ASP227 | |
| C | LYS180 | |
| C | GLY205 | |
| C | ASP227 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 12 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:27422871, ECO:0007744|PDB:3BGV, ECO:0007744|PDB:5E9J, ECO:0007744|PDB:5E9W |
| Chain | Residue | Details |
| A | ASP261 | |
| D | ASP261 | |
| D | GLN284 | |
| D | TYR289 | |
| A | GLN284 | |
| A | TYR289 | |
| B | ASP261 | |
| B | GLN284 | |
| B | TYR289 | |
| C | ASP261 | |
| C | GLN284 | |
| C | TYR289 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 24 |
| Details | SITE: mRNA cap binding => ECO:0000255|PROSITE-ProRule:PRU00895 |
| Chain | Residue | Details |
| A | LYS208 | |
| B | HIS288 | |
| B | GLU370 | |
| B | TYR467 | |
| C | LYS208 | |
| C | LYS214 | |
| C | ARG239 | |
| C | HIS288 | |
| C | GLU370 | |
| C | TYR467 | |
| D | LYS208 | |
| A | LYS214 | |
| D | LYS214 | |
| D | ARG239 | |
| D | HIS288 | |
| D | GLU370 | |
| D | TYR467 | |
| A | ARG239 | |
| A | HIS288 | |
| A | GLU370 | |
| A | TYR467 | |
| B | LYS208 | |
| B | LYS214 | |
| B | ARG239 |
