5DFJ

Human APE1 E96Q/D210N mismatch substrate complex

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003677	molecular_function	DNA binding
A	0003824	molecular_function	catalytic activity
A	0004518	molecular_function	nuclease activity
A	0004519	molecular_function	endonuclease activity
A	0006281	biological_process	DNA repair
B	0003677	molecular_function	DNA binding
B	0003824	molecular_function	catalytic activity
B	0004518	molecular_function	nuclease activity
B	0004519	molecular_function	endonuclease activity
B	0006281	biological_process	DNA repair

Functional Information from PDB Data

site_id	AC1
Number of Residues	3
Details	binding site for residue CL A 401

Chain	Residue
A	GLN96
A	SER129
A	TYR171

---

site_id	AC2
Number of Residues	3
Details	binding site for residue CL A 402

Chain	Residue
A	GLU154
A	ARG156
A	TRP188

---

site_id	AC3
Number of Residues	5
Details	binding site for residue CL A 403

Chain	Residue
A	HOH638
A	HOH706
A	PHE253
A	ARG281
A	ASP283

---

site_id	AC4
Number of Residues	4
Details	binding site for residue CL A 404

Chain	Residue
A	HIS215
A	ASP251
A	HOH583
A	HOH800

---

site_id	AC5
Number of Residues	4
Details	binding site for residue CL A 405

Chain	Residue
A	ASP47
A	TYR257
A	SER298
A	LYS299

---

site_id	AC6
Number of Residues	5
Details	binding site for residue EDO A 406

Chain	Residue
A	ASP189
A	PHE192
A	GLY239
A	PHE240
A	HOH541

---

site_id	AC7
Number of Residues	6
Details	binding site for residue EDO A 407

Chain	Residue
A	LEU44
A	TYR45
A	ASN277
A	HOH570
A	HOH589
A	HOH675

---

site_id	AC8
Number of Residues	3
Details	binding site for residue CL B 401

Chain	Residue
A	HOH702
B	HIS116
B	GLN117

---

site_id	AC9
Number of Residues	3
Details	binding site for residue CL B 402

Chain	Residue
B	GLY145
B	ARG156
B	VAL157

---

site_id	AD1
Number of Residues	3
Details	binding site for residue CL V 101

Chain	Residue
A	MET271
V	DG11
V	DG12

---

Functional Information from PROSITE/UniProt

site_id	PS00727
Number of Residues	17
Details	AP_NUCLEASE_F1_2 AP endonucleases family 1 signature 2. DSFRHlypntpyaYTFW

Chain	Residue	Details
A	ASP251-TRP267

---

site_id	PS00728
Number of Residues	12
Details	AP_NUCLEASE_F1_3 AP endonucleases family 1 signature 3. NvGwRLDYfLlS

Chain	Residue	Details
A	ASN277-SER288

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	58
Details	Region: {"description":"Mitochondrial targeting sequence (MTS)"}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	32
Details	Motif: {"description":"Nuclear export signal (NES)"}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	Active site: {"evidences":[{"source":"PubMed","id":"15380100","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	Active site: {"description":"Proton donor/acceptor","evidences":[{"source":"PubMed","id":"9351835","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1BIX","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	2
Details	Active site: {"description":"Proton acceptor","evidences":[{"source":"PROSITE-ProRule","id":"PRU00764","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	12
Details	Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU00764","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	2
Details	Site: {"description":"Transition state stabilizer","evidences":[{"source":"PubMed","id":"8932375","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	2
Details	Site: {"description":"Important for catalytic activity","evidences":[{"source":"PubMed","id":"21762700","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"9804799","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	2
Details	Site: {"description":"Interaction with DNA substrate"}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	2
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI11
Number of Residues	4
Details	Modified residue: {"description":"S-nitrosocysteine; alternate","evidences":[{"source":"PubMed","id":"17403694","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI12
Number of Residues	2
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"PubMed","id":"19608861","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI13
Number of Residues	2
Details	Modified residue: {"description":"Phosphothreonine; by CDK5","evidences":[{"source":"UniProtKB","id":"P28352","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI14
Number of Residues	2
Details	Modified residue: {"description":"S-nitrosocysteine","evidences":[{"source":"PubMed","id":"17403694","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	8
Details	M-CSA 510

Chain	Residue	Details
A	ASP70	metal ligand
A	GLN96	metal ligand
A	TYR171	electrostatic stabiliser, metal ligand
A	ASN210	increase nucleophilicity, metal ligand, proton acceptor
A	ASN212
A	ASP283	electrostatic stabiliser
A	ASP308	metal ligand
A	HIS309	electrostatic stabiliser, metal ligand

---

site_id	MCSA2
Number of Residues	8
Details	M-CSA 510

Chain	Residue	Details
B	ASP70	metal ligand
B	GLN96	metal ligand
B	TYR171	electrostatic stabiliser, metal ligand
B	ASN210	increase nucleophilicity, metal ligand, proton acceptor
B	ASN212
B	ASP283	electrostatic stabiliser
B	ASP308	metal ligand
B	HIS309	electrostatic stabiliser, metal ligand

---