5DDI
Crystal structure of glyceraldehyde-3-phosphate dehydrogenase from pig muscle - holo enzyme - at 2.40 Angstrom resolution
Functional Information from GO Data
Chain | GOid | namespace | contents |
P | 0000226 | biological_process | microtubule cytoskeleton organization |
P | 0004365 | molecular_function | glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity |
P | 0005634 | cellular_component | nucleus |
P | 0005737 | cellular_component | cytoplasm |
P | 0005829 | cellular_component | cytosol |
P | 0005856 | cellular_component | cytoskeleton |
P | 0006006 | biological_process | glucose metabolic process |
P | 0006096 | biological_process | glycolytic process |
P | 0006417 | biological_process | regulation of translation |
P | 0006915 | biological_process | apoptotic process |
P | 0008017 | molecular_function | microtubule binding |
P | 0015630 | cellular_component | microtubule cytoskeleton |
P | 0016491 | molecular_function | oxidoreductase activity |
P | 0016620 | molecular_function | oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor |
P | 0016740 | molecular_function | transferase activity |
P | 0032481 | biological_process | positive regulation of type I interferon production |
P | 0035605 | molecular_function | peptidyl-cysteine S-nitrosylase activity |
P | 0035606 | biological_process | peptidyl-cysteine S-trans-nitrosylation |
P | 0043123 | biological_process | positive regulation of canonical NF-kappaB signal transduction |
P | 0045087 | biological_process | innate immune response |
P | 0050661 | molecular_function | NADP binding |
P | 0050821 | biological_process | protein stabilization |
P | 0051287 | molecular_function | NAD binding |
P | 0051402 | biological_process | neuron apoptotic process |
P | 0097452 | cellular_component | GAIT complex |
R | 0000226 | biological_process | microtubule cytoskeleton organization |
R | 0004365 | molecular_function | glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity |
R | 0005634 | cellular_component | nucleus |
R | 0005737 | cellular_component | cytoplasm |
R | 0005829 | cellular_component | cytosol |
R | 0005856 | cellular_component | cytoskeleton |
R | 0006006 | biological_process | glucose metabolic process |
R | 0006096 | biological_process | glycolytic process |
R | 0006417 | biological_process | regulation of translation |
R | 0006915 | biological_process | apoptotic process |
R | 0008017 | molecular_function | microtubule binding |
R | 0015630 | cellular_component | microtubule cytoskeleton |
R | 0016491 | molecular_function | oxidoreductase activity |
R | 0016620 | molecular_function | oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor |
R | 0016740 | molecular_function | transferase activity |
R | 0032481 | biological_process | positive regulation of type I interferon production |
R | 0035605 | molecular_function | peptidyl-cysteine S-nitrosylase activity |
R | 0035606 | biological_process | peptidyl-cysteine S-trans-nitrosylation |
R | 0043123 | biological_process | positive regulation of canonical NF-kappaB signal transduction |
R | 0045087 | biological_process | innate immune response |
R | 0050661 | molecular_function | NADP binding |
R | 0050821 | biological_process | protein stabilization |
R | 0051287 | molecular_function | NAD binding |
R | 0051402 | biological_process | neuron apoptotic process |
R | 0097452 | cellular_component | GAIT complex |
S | 0000226 | biological_process | microtubule cytoskeleton organization |
S | 0004365 | molecular_function | glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity |
S | 0005634 | cellular_component | nucleus |
S | 0005737 | cellular_component | cytoplasm |
S | 0005829 | cellular_component | cytosol |
S | 0005856 | cellular_component | cytoskeleton |
S | 0006006 | biological_process | glucose metabolic process |
S | 0006096 | biological_process | glycolytic process |
S | 0006417 | biological_process | regulation of translation |
S | 0006915 | biological_process | apoptotic process |
S | 0008017 | molecular_function | microtubule binding |
S | 0015630 | cellular_component | microtubule cytoskeleton |
S | 0016491 | molecular_function | oxidoreductase activity |
S | 0016620 | molecular_function | oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor |
S | 0016740 | molecular_function | transferase activity |
S | 0032481 | biological_process | positive regulation of type I interferon production |
S | 0035605 | molecular_function | peptidyl-cysteine S-nitrosylase activity |
S | 0035606 | biological_process | peptidyl-cysteine S-trans-nitrosylation |
S | 0043123 | biological_process | positive regulation of canonical NF-kappaB signal transduction |
S | 0045087 | biological_process | innate immune response |
S | 0050661 | molecular_function | NADP binding |
S | 0050821 | biological_process | protein stabilization |
S | 0051287 | molecular_function | NAD binding |
S | 0051402 | biological_process | neuron apoptotic process |
S | 0097452 | cellular_component | GAIT complex |
Functional Information from PDB Data
site_id | AC1 |
Number of Residues | 6 |
Details | binding site for residue SO4 P 501 |
Chain | Residue |
P | SER148 |
P | THR208 |
P | GLY209 |
P | ALA210 |
P | HOH620 |
P | HOH640 |
site_id | AC2 |
Number of Residues | 3 |
Details | binding site for residue SO4 P 502 |
Chain | Residue |
P | THR179 |
P | THR181 |
P | ARG231 |
site_id | AC3 |
Number of Residues | 16 |
Details | binding site for residue NAD P 503 |
Chain | Residue |
P | PHE8 |
P | GLY9 |
P | ARG10 |
P | ILE11 |
P | ASP32 |
P | PRO33 |
P | PHE34 |
P | ARG77 |
P | THR96 |
P | GLY97 |
P | SER119 |
P | ASN313 |
P | HOH605 |
P | HOH613 |
P | HOH657 |
R | HOH610 |
site_id | AC4 |
Number of Residues | 4 |
Details | binding site for residue SO4 R 501 |
Chain | Residue |
R | SER148 |
R | THR208 |
R | GLY209 |
R | ALA210 |
site_id | AC5 |
Number of Residues | 4 |
Details | binding site for residue SO4 R 502 |
Chain | Residue |
R | THR179 |
R | THR181 |
R | ARG231 |
R | NAD503 |
site_id | AC6 |
Number of Residues | 13 |
Details | binding site for residue NAD R 503 |
Chain | Residue |
R | GLY9 |
R | ARG10 |
R | ILE11 |
R | ASN31 |
R | ASP32 |
R | PRO33 |
R | ARG77 |
R | SER95 |
R | THR96 |
R | GLY97 |
R | SER119 |
R | ASN313 |
R | SO4502 |
site_id | AC7 |
Number of Residues | 3 |
Details | binding site for residue SO4 S 501 |
Chain | Residue |
S | THR208 |
S | GLY209 |
S | ALA210 |
site_id | AC8 |
Number of Residues | 6 |
Details | binding site for residue SO4 S 502 |
Chain | Residue |
S | THR179 |
S | THR181 |
S | ARG231 |
S | NAD503 |
S | HOH615 |
S | HOH624 |
site_id | AC9 |
Number of Residues | 18 |
Details | binding site for residue NAD S 503 |
Chain | Residue |
S | PHE8 |
S | GLY9 |
S | ARG10 |
S | ILE11 |
S | ASP32 |
S | PRO33 |
S | PHE34 |
S | ARG77 |
S | THR96 |
S | GLY97 |
S | SER119 |
S | ALA120 |
S | ASN313 |
S | SO4502 |
S | HOH620 |
S | HOH624 |
S | HOH625 |
S | HOH666 |
Functional Information from PROSITE/UniProt
site_id | PS00071 |
Number of Residues | 8 |
Details | GAPDH Glyceraldehyde 3-phosphate dehydrogenase active site. ASCTTNcL |
Chain | Residue | Details |
P | ALA147-LEU154 |
Functional Information from SwissProt/UniProt
site_id | SWS_FT_FI1 |
Number of Residues | 3 |
Details | ACT_SITE: Nucleophile => ECO:0000255|PROSITE-ProRule:PRU10009 |
Chain | Residue | Details |
P | CYS149 | |
R | CYS149 | |
S | CYS149 |
site_id | SWS_FT_FI2 |
Number of Residues | 15 |
Details | BINDING: BINDING => ECO:0000250|UniProtKB:P04406 |
Chain | Residue | Details |
P | ARG10 | |
R | ASN313 | |
S | ARG10 | |
S | ASP32 | |
S | ARG77 | |
S | SER119 | |
S | ASN313 | |
P | ASP32 | |
P | ARG77 | |
P | SER119 | |
P | ASN313 | |
R | ARG10 | |
R | ASP32 | |
R | ARG77 | |
R | SER119 |
site_id | SWS_FT_FI3 |
Number of Residues | 12 |
Details | BINDING: BINDING => ECO:0000250|UniProtKB:P22513 |
Chain | Residue | Details |
P | SER148 | |
S | THR179 | |
S | THR208 | |
S | ARG231 | |
P | THR179 | |
P | THR208 | |
P | ARG231 | |
R | SER148 | |
R | THR179 | |
R | THR208 | |
R | ARG231 | |
S | SER148 |
site_id | SWS_FT_FI4 |
Number of Residues | 3 |
Details | SITE: Activates thiol group during catalysis => ECO:0000250|UniProtKB:P04406 |
Chain | Residue | Details |
P | HIS176 | |
R | HIS176 | |
S | HIS176 |
site_id | SWS_FT_FI5 |
Number of Residues | 15 |
Details | MOD_RES: N6,N6-dimethyllysine => ECO:0000250|UniProtKB:P04406 |
Chain | Residue | Details |
P | LYS2 | |
R | LYS331 | |
S | LYS2 | |
S | LYS63 | |
S | GLN257 | |
S | LYS260 | |
S | LYS331 | |
P | LYS63 | |
P | GLN257 | |
P | LYS260 | |
P | LYS331 | |
R | LYS2 | |
R | LYS63 | |
R | GLN257 | |
R | LYS260 |
site_id | SWS_FT_FI6 |
Number of Residues | 21 |
Details | MOD_RES: Deamidated asparagine => ECO:0000250|UniProtKB:P04406 |
Chain | Residue | Details |
P | ASN6 | |
R | ASN67 | |
R | ASN146 | |
R | ASN152 | |
R | ASN222 | |
R | ASN313 | |
S | ASN6 | |
S | ASN61 | |
S | ASN67 | |
S | ASN146 | |
S | ASN152 | |
P | ASN61 | |
S | ASN222 | |
S | ASN313 | |
P | ASN67 | |
P | ASN146 | |
P | ASN152 | |
P | ASN222 | |
P | ASN313 | |
R | ASN6 | |
R | ASN61 |
site_id | SWS_FT_FI7 |
Number of Residues | 3 |
Details | MOD_RES: Phosphotyrosine => ECO:0000250|UniProtKB:P04406 |
Chain | Residue | Details |
P | TYR39 | |
R | TYR39 | |
S | TYR39 |
site_id | SWS_FT_FI8 |
Number of Residues | 9 |
Details | MOD_RES: N6-acetyllysine => ECO:0000250|UniProtKB:P04406 |
Chain | Residue | Details |
P | LYS58 | |
P | LYS216 | |
P | LYS251 | |
R | LYS58 | |
R | LYS216 | |
R | LYS251 | |
S | LYS58 | |
S | LYS216 | |
S | LYS251 |
site_id | SWS_FT_FI9 |
Number of Residues | 24 |
Details | MOD_RES: Phosphothreonine => ECO:0000250|UniProtKB:P04406 |
Chain | Residue | Details |
P | THR72 | |
R | THR150 | |
R | THR174 | |
R | THR179 | |
R | THR181 | |
R | THR208 | |
R | THR226 | |
R | THR234 | |
S | THR72 | |
S | THR150 | |
S | THR174 | |
P | THR150 | |
S | THR179 | |
S | THR181 | |
S | THR208 | |
S | THR226 | |
S | THR234 | |
P | THR174 | |
P | THR179 | |
P | THR181 | |
P | THR208 | |
P | THR226 | |
P | THR234 | |
R | THR72 |
site_id | SWS_FT_FI10 |
Number of Residues | 18 |
Details | MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:P04406 |
Chain | Residue | Details |
P | SER119 | |
R | SER238 | |
R | SER309 | |
R | SER330 | |
S | SER119 | |
S | SER145 | |
S | SER148 | |
S | SER238 | |
S | SER309 | |
S | SER330 | |
P | SER145 | |
P | SER148 | |
P | SER238 | |
P | SER309 | |
P | SER330 | |
R | SER119 | |
R | SER145 | |
R | SER148 |
site_id | SWS_FT_FI11 |
Number of Residues | 3 |
Details | MOD_RES: S-nitrosocysteine; in reversibly inhibited form => ECO:0000250|UniProtKB:P04797 |
Chain | Residue | Details |
P | CYS149 | |
R | CYS149 | |
S | CYS149 |
site_id | SWS_FT_FI12 |
Number of Residues | 6 |
Details | MOD_RES: N6-malonyllysine; alternate => ECO:0000250|UniProtKB:P04406 |
Chain | Residue | Details |
P | LYS191 | |
P | LYS212 | |
R | LYS191 | |
R | LYS212 | |
S | LYS191 | |
S | LYS212 |
site_id | SWS_FT_FI13 |
Number of Residues | 3 |
Details | MOD_RES: N6-acetyllysine; alternate => ECO:0000250|UniProtKB:P04406 |
Chain | Residue | Details |
P | LYS224 | |
R | LYS224 | |
S | LYS224 |
site_id | SWS_FT_FI14 |
Number of Residues | 3 |
Details | MOD_RES: S-nitrosocysteine => ECO:0000250|UniProtKB:P04406 |
Chain | Residue | Details |
P | CYS244 | |
R | CYS244 | |
S | CYS244 |
site_id | SWS_FT_FI15 |
Number of Residues | 6 |
Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0000250|UniProtKB:P04406 |
Chain | Residue | Details |
P | LYS183 | |
R | LYS183 | |
S | LYS183 |