5D51
Krypton derivatization of an O2-tolerant membrane-bound [NiFe] hydrogenase reveals a hydrophobic gas tunnel network
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| L | 0005515 | molecular_function | protein binding |
| L | 0005886 | cellular_component | plasma membrane |
| L | 0008901 | molecular_function | ferredoxin hydrogenase activity |
| L | 0016151 | molecular_function | nickel cation binding |
| L | 0016491 | molecular_function | oxidoreductase activity |
| L | 0033748 | molecular_function | hydrogenase (acceptor) activity |
| L | 0046872 | molecular_function | metal ion binding |
| S | 0005515 | molecular_function | protein binding |
| S | 0005886 | cellular_component | plasma membrane |
| S | 0008901 | molecular_function | ferredoxin hydrogenase activity |
| S | 0009055 | molecular_function | electron transfer activity |
| S | 0009061 | biological_process | anaerobic respiration |
| S | 0009375 | cellular_component | ferredoxin hydrogenase complex |
| S | 0016020 | cellular_component | membrane |
| S | 0016491 | molecular_function | oxidoreductase activity |
| S | 0033748 | molecular_function | hydrogenase (acceptor) activity |
| S | 0044569 | cellular_component | [Ni-Fe] hydrogenase complex |
| S | 0046872 | molecular_function | metal ion binding |
| S | 0051536 | molecular_function | iron-sulfur cluster binding |
| S | 0051538 | molecular_function | 3 iron, 4 sulfur cluster binding |
| S | 0051539 | molecular_function | 4 iron, 4 sulfur cluster binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 13 |
| Details | binding site for residue NFV L 701 |
| Chain | Residue |
| L | CYS75 |
| L | PRO552 |
| L | THR553 |
| L | CYS597 |
| L | CYS600 |
| L | CYS78 |
| L | CYS81 |
| L | HIS82 |
| L | ALA528 |
| L | PRO529 |
| L | ARG530 |
| L | LEU533 |
| L | VAL551 |
| site_id | AC2 |
| Number of Residues | 6 |
| Details | binding site for residue MG L 702 |
| Chain | Residue |
| L | GLU56 |
| L | CYS549 |
| L | HIS603 |
| L | HOH806 |
| L | HOH807 |
| L | HOH825 |
| site_id | AC3 |
| Number of Residues | 2 |
| Details | binding site for residue KR L 703 |
| Chain | Residue |
| L | ILE283 |
| L | GLY284 |
| site_id | AC4 |
| Number of Residues | 2 |
| Details | binding site for residue KR L 704 |
| Chain | Residue |
| L | VAL46 |
| L | LEU573 |
| site_id | AC5 |
| Number of Residues | 4 |
| Details | binding site for residue KR L 706 |
| Chain | Residue |
| L | VAL115 |
| L | TRP216 |
| L | PHE271 |
| L | ASN272 |
| site_id | AC6 |
| Number of Residues | 4 |
| Details | binding site for residue KR L 707 |
| Chain | Residue |
| L | ALA442 |
| L | LEU462 |
| L | LEU465 |
| L | LEU474 |
| site_id | AC7 |
| Number of Residues | 3 |
| Details | binding site for residue KR L 708 |
| Chain | Residue |
| L | PHE182 |
| L | TYR206 |
| L | KR713 |
| site_id | AC8 |
| Number of Residues | 3 |
| Details | binding site for residue KR L 709 |
| Chain | Residue |
| L | ALA198 |
| L | ASP279 |
| L | ALA282 |
| site_id | AC9 |
| Number of Residues | 1 |
| Details | binding site for residue KR L 711 |
| Chain | Residue |
| L | PHE182 |
| site_id | AD1 |
| Number of Residues | 2 |
| Details | binding site for residue KR L 712 |
| Chain | Residue |
| L | LEU170 |
| L | KR713 |
| site_id | AD2 |
| Number of Residues | 3 |
| Details | binding site for residue KR L 713 |
| Chain | Residue |
| L | LEU207 |
| L | KR708 |
| L | KR712 |
| site_id | AD3 |
| Number of Residues | 5 |
| Details | binding site for residue KR L 714 |
| Chain | Residue |
| L | ALA442 |
| L | ASN446 |
| L | TYR460 |
| L | THR461 |
| L | LEU465 |
| site_id | AD4 |
| Number of Residues | 4 |
| Details | binding site for residue CL L 715 |
| Chain | Residue |
| L | ARG88 |
| L | ARG106 |
| L | HOH824 |
| L | HOH1148 |
| site_id | AD5 |
| Number of Residues | 6 |
| Details | binding site for residue SF4 S 1001 |
| Chain | Residue |
| S | HIS187 |
| S | CYS190 |
| S | ARG193 |
| S | CYS215 |
| S | LEU216 |
| S | CYS221 |
| site_id | AD6 |
| Number of Residues | 9 |
| Details | binding site for residue F3S S 1002 |
| Chain | Residue |
| L | LYS226 |
| S | THR226 |
| S | ASN228 |
| S | CYS230 |
| S | TRP235 |
| S | PRO242 |
| S | CYS249 |
| S | ILE250 |
| S | CYS252 |
| site_id | AD7 |
| Number of Residues | 10 |
| Details | binding site for residue F4S S 1003 |
| Chain | Residue |
| S | CYS17 |
| S | THR18 |
| S | CYS19 |
| S | CYS20 |
| S | GLU76 |
| S | SER114 |
| S | CYS115 |
| S | CYS120 |
| S | GLY148 |
| S | CYS149 |
| site_id | AD8 |
| Number of Residues | 1 |
| Details | binding site for residue KR S 1004 |
| Chain | Residue |
| S | THR47 |
| site_id | AD9 |
| Number of Residues | 2 |
| Details | binding site for residue KR S 1006 |
| Chain | Residue |
| S | PHE24 |
| S | KR1009 |
| site_id | AE1 |
| Number of Residues | 3 |
| Details | binding site for residue KR S 1007 |
| Chain | Residue |
| L | VAL77 |
| S | GLU22 |
| S | THR47 |
| site_id | AE2 |
| Number of Residues | 3 |
| Details | binding site for residue KR S 1008 |
| Chain | Residue |
| S | TRP11 |
| S | ILE40 |
| S | ILE160 |
| site_id | AE3 |
| Number of Residues | 3 |
| Details | binding site for residue KR S 1009 |
| Chain | Residue |
| L | LEU210 |
| S | VAL36 |
| S | KR1006 |
| site_id | AE4 |
| Number of Residues | 3 |
| Details | binding site for residue KR S 1010 |
| Chain | Residue |
| S | ASP167 |
| S | ARG168 |
| S | HOH1198 |
| site_id | AE5 |
| Number of Residues | 3 |
| Details | binding site for residue CL S 1011 |
| Chain | Residue |
| S | TRP118 |
| S | CYS120 |
| S | GLY256 |
Functional Information from PROSITE/UniProt
| site_id | PS00507 |
| Number of Residues | 26 |
| Details | NI_HGENASE_L_1 Nickel-dependent hydrogenases large subunit signature 1. RGLEvilkgrdprdawafvERiCGVC |
| Chain | Residue | Details |
| L | ARG53-CYS78 |
| site_id | PS00508 |
| Number of Residues | 10 |
| Details | NI_HGENASE_L_2 Nickel-dependent hydrogenases large subunit signature 2. FDPCLACst.H |
| Chain | Residue | Details |
| L | PHE594-HIS603 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 11 |
| Details | BINDING: BINDING => ECO:0000250|UniProtKB:P21853 |
| Chain | Residue | Details |
| S | CYS17 | |
| S | CYS249 | |
| S | CYS252 | |
| S | CYS20 | |
| S | CYS115 | |
| S | CYS149 | |
| S | HIS187 | |
| S | CYS190 | |
| S | CYS215 | |
| S | CYS221 | |
| S | CYS230 |
