5D51

Krypton derivatization of an O2-tolerant membrane-bound [NiFe] hydrogenase reveals a hydrophobic gas tunnel network

Functional Information from GO Data

Chain	GOid	namespace	contents
L	0005515	molecular_function	protein binding
L	0005886	cellular_component	plasma membrane
L	0008901	molecular_function	ferredoxin hydrogenase activity
L	0016151	molecular_function	nickel cation binding
L	0016491	molecular_function	oxidoreductase activity
L	0033748	molecular_function	hydrogenase (acceptor) activity
L	0046872	molecular_function	metal ion binding
S	0005515	molecular_function	protein binding
S	0005886	cellular_component	plasma membrane
S	0008901	molecular_function	ferredoxin hydrogenase activity
S	0009055	molecular_function	electron transfer activity
S	0009061	biological_process	anaerobic respiration
S	0009375	cellular_component	ferredoxin hydrogenase complex
S	0016020	cellular_component	membrane
S	0016491	molecular_function	oxidoreductase activity
S	0033748	molecular_function	hydrogenase (acceptor) activity
S	0044569	cellular_component	[Ni-Fe] hydrogenase complex
S	0046872	molecular_function	metal ion binding
S	0051536	molecular_function	iron-sulfur cluster binding
S	0051538	molecular_function	3 iron, 4 sulfur cluster binding
S	0051539	molecular_function	4 iron, 4 sulfur cluster binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	13
Details	binding site for residue NFV L 701

Chain	Residue
L	CYS75
L	PRO552
L	THR553
L	CYS597
L	CYS600
L	CYS78
L	CYS81
L	HIS82
L	ALA528
L	PRO529
L	ARG530
L	LEU533
L	VAL551

---

site_id	AC2
Number of Residues	6
Details	binding site for residue MG L 702

Chain	Residue
L	GLU56
L	CYS549
L	HIS603
L	HOH806
L	HOH807
L	HOH825

---

site_id	AC3
Number of Residues	2
Details	binding site for residue KR L 703

Chain	Residue
L	ILE283
L	GLY284

---

site_id	AC4
Number of Residues	2
Details	binding site for residue KR L 704

Chain	Residue
L	VAL46
L	LEU573

---

site_id	AC5
Number of Residues	4
Details	binding site for residue KR L 706

Chain	Residue
L	VAL115
L	TRP216
L	PHE271
L	ASN272

---

site_id	AC6
Number of Residues	4
Details	binding site for residue KR L 707

Chain	Residue
L	ALA442
L	LEU462
L	LEU465
L	LEU474

---

site_id	AC7
Number of Residues	3
Details	binding site for residue KR L 708

Chain	Residue
L	PHE182
L	TYR206
L	KR713

---

site_id	AC8
Number of Residues	3
Details	binding site for residue KR L 709

Chain	Residue
L	ALA198
L	ASP279
L	ALA282

---

site_id	AC9
Number of Residues	1
Details	binding site for residue KR L 711

Chain	Residue
L	PHE182

---

site_id	AD1
Number of Residues	2
Details	binding site for residue KR L 712

Chain	Residue
L	LEU170
L	KR713

---

site_id	AD2
Number of Residues	3
Details	binding site for residue KR L 713

Chain	Residue
L	LEU207
L	KR708
L	KR712

---

site_id	AD3
Number of Residues	5
Details	binding site for residue KR L 714

Chain	Residue
L	ALA442
L	ASN446
L	TYR460
L	THR461
L	LEU465

---

site_id	AD4
Number of Residues	4
Details	binding site for residue CL L 715

Chain	Residue
L	ARG88
L	ARG106
L	HOH824
L	HOH1148

---

site_id	AD5
Number of Residues	6
Details	binding site for residue SF4 S 1001

Chain	Residue
S	HIS187
S	CYS190
S	ARG193
S	CYS215
S	LEU216
S	CYS221

---

site_id	AD6
Number of Residues	9
Details	binding site for residue F3S S 1002

Chain	Residue
L	LYS226
S	THR226
S	ASN228
S	CYS230
S	TRP235
S	PRO242
S	CYS249
S	ILE250
S	CYS252

---

site_id	AD7
Number of Residues	10
Details	binding site for residue F4S S 1003

Chain	Residue
S	CYS17
S	THR18
S	CYS19
S	CYS20
S	GLU76
S	SER114
S	CYS115
S	CYS120
S	GLY148
S	CYS149

---

site_id	AD8
Number of Residues	1
Details	binding site for residue KR S 1004

Chain	Residue
S	THR47

---

site_id	AD9
Number of Residues	2
Details	binding site for residue KR S 1006

Chain	Residue
S	PHE24
S	KR1009

---

site_id	AE1
Number of Residues	3
Details	binding site for residue KR S 1007

Chain	Residue
L	VAL77
S	GLU22
S	THR47

---

site_id	AE2
Number of Residues	3
Details	binding site for residue KR S 1008

Chain	Residue
S	TRP11
S	ILE40
S	ILE160

---

site_id	AE3
Number of Residues	3
Details	binding site for residue KR S 1009

Chain	Residue
L	LEU210
S	VAL36
S	KR1006

---

site_id	AE4
Number of Residues	3
Details	binding site for residue KR S 1010

Chain	Residue
S	ASP167
S	ARG168
S	HOH1198

---

site_id	AE5
Number of Residues	3
Details	binding site for residue CL S 1011

Chain	Residue
S	TRP118
S	CYS120
S	GLY256

---

Functional Information from PROSITE/UniProt

site_id	PS00507
Number of Residues	26
Details	NI_HGENASE_L_1 Nickel-dependent hydrogenases large subunit signature 1. RGLEvilkgrdprdawafvERiCGVC

Chain	Residue	Details
L	ARG53-CYS78

---

site_id	PS00508
Number of Residues	10
Details	NI_HGENASE_L_2 Nickel-dependent hydrogenases large subunit signature 2. FDPCLACst.H

Chain	Residue	Details
L	PHE594-HIS603

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	Binding site: {"evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	11
Details	Binding site: {"evidences":[{"source":"UniProtKB","id":"P21853","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---