5D40
Crystal structure of the 5-selective H176Y mutant of Cytochrome TxtE
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004497 | molecular_function | monooxygenase activity |
| A | 0005506 | molecular_function | iron ion binding |
| A | 0006707 | biological_process | cholesterol catabolic process |
| A | 0008395 | molecular_function | steroid hydroxylase activity |
| A | 0016705 | molecular_function | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen |
| A | 0020037 | molecular_function | heme binding |
| A | 0036199 | molecular_function | cholest-4-en-3-one 26-monooxygenase activity |
| A | 0046872 | molecular_function | metal ion binding |
| B | 0004497 | molecular_function | monooxygenase activity |
| B | 0005506 | molecular_function | iron ion binding |
| B | 0006707 | biological_process | cholesterol catabolic process |
| B | 0008395 | molecular_function | steroid hydroxylase activity |
| B | 0016705 | molecular_function | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen |
| B | 0020037 | molecular_function | heme binding |
| B | 0036199 | molecular_function | cholest-4-en-3-one 26-monooxygenase activity |
| B | 0046872 | molecular_function | metal ion binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 23 |
| Details | binding site for residue HEM A 501 |
| Chain | Residue |
| A | ARG59 |
| A | SER254 |
| A | THR296 |
| A | ARG298 |
| A | SER349 |
| A | GLY351 |
| A | VAL354 |
| A | HIS355 |
| A | CYS357 |
| A | LEU358 |
| A | ALA359 |
| A | VAL87 |
| A | ILE363 |
| A | TRP502 |
| A | HOH704 |
| A | HOH884 |
| A | MET88 |
| A | HIS95 |
| A | ARG99 |
| A | PHE155 |
| A | ALA245 |
| A | THR250 |
| A | THR251 |
| site_id | AC2 |
| Number of Residues | 11 |
| Details | binding site for residue TRP A 502 |
| Chain | Residue |
| A | ARG59 |
| A | MET88 |
| A | TYR89 |
| A | TYR176 |
| A | ASN293 |
| A | THR296 |
| A | TRP297 |
| A | PHE395 |
| A | HEM501 |
| A | HOH659 |
| A | HOH670 |
| site_id | AC3 |
| Number of Residues | 10 |
| Details | binding site for residue GOL A 503 |
| Chain | Residue |
| A | LEU50 |
| A | ASP52 |
| A | LEU55 |
| A | THR90 |
| A | ASP91 |
| A | PRO92 |
| A | ARG298 |
| A | HIS355 |
| A | HOH677 |
| A | HOH692 |
| site_id | AC4 |
| Number of Residues | 7 |
| Details | binding site for residue GOL A 504 |
| Chain | Residue |
| A | ASN284 |
| A | ALA360 |
| A | GLN361 |
| A | SER364 |
| A | HOH612 |
| A | HOH712 |
| A | HOH895 |
| site_id | AC5 |
| Number of Residues | 23 |
| Details | binding site for residue HEM B 501 |
| Chain | Residue |
| B | ARG59 |
| B | VAL87 |
| B | MET88 |
| B | HIS95 |
| B | ARG99 |
| B | PHE155 |
| B | ALA245 |
| B | THR250 |
| B | THR251 |
| B | SER254 |
| B | THR296 |
| B | ARG298 |
| B | SER349 |
| B | GLY351 |
| B | VAL354 |
| B | HIS355 |
| B | CYS357 |
| B | ALA359 |
| B | ILE363 |
| B | TRP502 |
| B | HOH665 |
| B | HOH671 |
| B | HOH802 |
| site_id | AC6 |
| Number of Residues | 12 |
| Details | binding site for residue TRP B 502 |
| Chain | Residue |
| B | ARG59 |
| B | MET88 |
| B | TYR89 |
| B | TYR176 |
| B | ASN293 |
| B | THR296 |
| B | TRP297 |
| B | PHE395 |
| B | HEM501 |
| B | HOH644 |
| B | HOH665 |
| B | HOH679 |
| site_id | AC7 |
| Number of Residues | 12 |
| Details | binding site for residue GOL B 503 |
| Chain | Residue |
| A | ARG267 |
| B | PRO14 |
| B | TYR17 |
| B | ARG264 |
| B | TYR289 |
| B | ASN290 |
| B | ARG326 |
| B | TRP389 |
| B | ARG396 |
| B | GOL504 |
| B | CL510 |
| B | HOH782 |
| site_id | AC8 |
| Number of Residues | 10 |
| Details | binding site for residue GOL B 504 |
| Chain | Residue |
| A | PRO266 |
| A | ARG267 |
| A | HOH855 |
| A | HOH875 |
| B | ASP15 |
| B | PRO18 |
| B | TYR289 |
| B | ARG326 |
| B | GOL503 |
| A | ARG264 |
| site_id | AC9 |
| Number of Residues | 10 |
| Details | binding site for residue GOL B 505 |
| Chain | Residue |
| B | LEU50 |
| B | ASP52 |
| B | LEU55 |
| B | THR90 |
| B | ASP91 |
| B | PRO92 |
| B | ARG298 |
| B | HIS355 |
| B | HOH678 |
| B | HOH726 |
| site_id | AD1 |
| Number of Residues | 6 |
| Details | binding site for residue GOL B 506 |
| Chain | Residue |
| A | ASP15 |
| A | TYR289 |
| A | ARG326 |
| A | HOH1007 |
| A | HOH1029 |
| B | PRO6 |
| site_id | AD2 |
| Number of Residues | 5 |
| Details | binding site for residue GOL B 507 |
| Chain | Residue |
| A | TRP389 |
| A | HOH1196 |
| B | PRO93 |
| B | ASP96 |
| B | ARG100 |
| site_id | AD3 |
| Number of Residues | 6 |
| Details | binding site for residue GOL B 508 |
| Chain | Residue |
| B | ASN284 |
| B | ALA360 |
| B | GLN361 |
| B | SER364 |
| B | HOH771 |
| B | HOH843 |
| site_id | AD4 |
| Number of Residues | 5 |
| Details | binding site for residue GOL B 509 |
| Chain | Residue |
| A | PRO93 |
| A | ASP96 |
| A | ARG100 |
| A | HOH1022 |
| B | TRP389 |
| site_id | AD5 |
| Number of Residues | 4 |
| Details | binding site for residue CL B 510 |
| Chain | Residue |
| B | ARG264 |
| B | TYR289 |
| B | GOL503 |
| B | HOH834 |
Functional Information from PROSITE/UniProt
| site_id | PS00086 |
| Number of Residues | 10 |
| Details | CYTOCHROME_P450 Cytochrome P450 cysteine heme-iron ligand signature. FGqGVHACLA |
| Chain | Residue | Details |
| A | PHE350-ALA359 |
