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5CV6

Crystal structure of Staphylococcal nuclease variant Delta+PHS V66H/I92E at cryogenic temperature

Functional Information from GO Data
ChainGOidnamespacecontents
A0003676molecular_functionnucleic acid binding
A0004518molecular_functionnuclease activity
Functional Information from PDB Data
site_idAC1
Number of Residues7
Detailsbinding site for residue CA A 201
ChainResidue
AASP21
AASP40
ATHR41
AGLU43
ATHP202
AHOH304
AHOH334

site_idAC2
Number of Residues22
Detailsbinding site for residue THP A 202
ChainResidue
ALYS84
ATYR85
AARG87
ALEU89
ATYR113
ATYR115
ALYS127
ACA201
AHOH304
AHOH305
AHOH307
AHOH310
AHOH328
AHOH330
AHOH335
AHOH336
AHOH352
AHOH363
AHOH368
AHOH376
AARG35
AASP40

Functional Information from PROSITE/UniProt
site_idPS01123
Number of Residues25
DetailsTNASE_1 Thermonuclease family signature 1. DGDTVkLmykgqpmtf.RLllVDtPE
ChainResidueDetails
AASP19-GLU43

Functional Information from SwissProt/UniProt
site_idSWS_FT_FI1
Number of Residues3
DetailsACT_SITE: ACT_SITE => ECO:0000269|PubMed:288045
ChainResidueDetails
AARG35
AGLU43
ATYR93

site_idSWS_FT_FI2
Number of Residues3
DetailsBINDING:
ChainResidueDetails
AASP21
AASP40
ATHR41

Catalytic Information from CSA
site_idMCSA1
Number of Residues6
DetailsM-CSA 165
ChainResidueDetails
AASP21metal ligand
AARG35electrostatic stabiliser, hydrogen bond donor
AASP40metal ligand
ATHR41metal ligand
AGLU43hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
ATYR93electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor

226707

PDB entries from 2024-10-30

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