5CHW

Structure of ISG15 in space group P212121

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0002682	biological_process	regulation of immune system process
A	0003735	molecular_function	structural constituent of ribosome
A	0005178	molecular_function	integrin binding
A	0005515	molecular_function	protein binding
A	0005576	cellular_component	extracellular region
A	0005634	cellular_component	nucleus
A	0005737	cellular_component	cytoplasm
A	0007229	biological_process	integrin-mediated signaling pathway
A	0009615	biological_process	response to virus
A	0009617	biological_process	response to bacterium
A	0019941	biological_process	modification-dependent protein catabolic process
A	0022626	cellular_component	cytosolic ribosome
A	0030501	biological_process	positive regulation of bone mineralization
A	0031386	molecular_function	protein tag activity
A	0031397	biological_process	negative regulation of protein ubiquitination
A	0032020	biological_process	ISG15-protein conjugation
A	0032461	biological_process	positive regulation of protein oligomerization
A	0032649	biological_process	regulation of type II interferon production
A	0032728	biological_process	positive regulation of interferon-beta production
A	0032729	biological_process	positive regulation of type II interferon production
A	0032733	biological_process	positive regulation of interleukin-10 production
A	0034340	biological_process	response to type I interferon
A	0042742	biological_process	defense response to bacterium
A	0045071	biological_process	negative regulation of viral genome replication
A	0045087	biological_process	innate immune response
A	0045648	biological_process	positive regulation of erythrocyte differentiation
A	0051240	biological_process	positive regulation of multicellular organismal process
A	0051607	biological_process	defense response to virus
A	0060339	biological_process	negative regulation of type I interferon-mediated signaling pathway
A	0070585	biological_process	protein localization to mitochondrion
B	0002682	biological_process	regulation of immune system process
B	0003735	molecular_function	structural constituent of ribosome
B	0005178	molecular_function	integrin binding
B	0005515	molecular_function	protein binding
B	0005576	cellular_component	extracellular region
B	0005634	cellular_component	nucleus
B	0005737	cellular_component	cytoplasm
B	0007229	biological_process	integrin-mediated signaling pathway
B	0009615	biological_process	response to virus
B	0009617	biological_process	response to bacterium
B	0019941	biological_process	modification-dependent protein catabolic process
B	0022626	cellular_component	cytosolic ribosome
B	0030501	biological_process	positive regulation of bone mineralization
B	0031386	molecular_function	protein tag activity
B	0031397	biological_process	negative regulation of protein ubiquitination
B	0032020	biological_process	ISG15-protein conjugation
B	0032461	biological_process	positive regulation of protein oligomerization
B	0032649	biological_process	regulation of type II interferon production
B	0032728	biological_process	positive regulation of interferon-beta production
B	0032729	biological_process	positive regulation of type II interferon production
B	0032733	biological_process	positive regulation of interleukin-10 production
B	0034340	biological_process	response to type I interferon
B	0042742	biological_process	defense response to bacterium
B	0045071	biological_process	negative regulation of viral genome replication
B	0045087	biological_process	innate immune response
B	0045648	biological_process	positive regulation of erythrocyte differentiation
B	0051240	biological_process	positive regulation of multicellular organismal process
B	0051607	biological_process	defense response to virus
B	0060339	biological_process	negative regulation of type I interferon-mediated signaling pathway
B	0070585	biological_process	protein localization to mitochondrion
C	0002682	biological_process	regulation of immune system process
C	0003735	molecular_function	structural constituent of ribosome
C	0005178	molecular_function	integrin binding
C	0005515	molecular_function	protein binding
C	0005576	cellular_component	extracellular region
C	0005634	cellular_component	nucleus
C	0005737	cellular_component	cytoplasm
C	0007229	biological_process	integrin-mediated signaling pathway
C	0009615	biological_process	response to virus
C	0009617	biological_process	response to bacterium
C	0019941	biological_process	modification-dependent protein catabolic process
C	0022626	cellular_component	cytosolic ribosome
C	0030501	biological_process	positive regulation of bone mineralization
C	0031386	molecular_function	protein tag activity
C	0031397	biological_process	negative regulation of protein ubiquitination
C	0032020	biological_process	ISG15-protein conjugation
C	0032461	biological_process	positive regulation of protein oligomerization
C	0032649	biological_process	regulation of type II interferon production
C	0032728	biological_process	positive regulation of interferon-beta production
C	0032729	biological_process	positive regulation of type II interferon production
C	0032733	biological_process	positive regulation of interleukin-10 production
C	0034340	biological_process	response to type I interferon
C	0042742	biological_process	defense response to bacterium
C	0045071	biological_process	negative regulation of viral genome replication
C	0045087	biological_process	innate immune response
C	0045648	biological_process	positive regulation of erythrocyte differentiation
C	0051240	biological_process	positive regulation of multicellular organismal process
C	0051607	biological_process	defense response to virus
C	0060339	biological_process	negative regulation of type I interferon-mediated signaling pathway
C	0070585	biological_process	protein localization to mitochondrion
D	0002682	biological_process	regulation of immune system process
D	0003735	molecular_function	structural constituent of ribosome
D	0005178	molecular_function	integrin binding
D	0005515	molecular_function	protein binding
D	0005576	cellular_component	extracellular region
D	0005634	cellular_component	nucleus
D	0005737	cellular_component	cytoplasm
D	0007229	biological_process	integrin-mediated signaling pathway
D	0009615	biological_process	response to virus
D	0009617	biological_process	response to bacterium
D	0019941	biological_process	modification-dependent protein catabolic process
D	0022626	cellular_component	cytosolic ribosome
D	0030501	biological_process	positive regulation of bone mineralization
D	0031386	molecular_function	protein tag activity
D	0031397	biological_process	negative regulation of protein ubiquitination
D	0032020	biological_process	ISG15-protein conjugation
D	0032461	biological_process	positive regulation of protein oligomerization
D	0032649	biological_process	regulation of type II interferon production
D	0032728	biological_process	positive regulation of interferon-beta production
D	0032729	biological_process	positive regulation of type II interferon production
D	0032733	biological_process	positive regulation of interleukin-10 production
D	0034340	biological_process	response to type I interferon
D	0042742	biological_process	defense response to bacterium
D	0045071	biological_process	negative regulation of viral genome replication
D	0045087	biological_process	innate immune response
D	0045648	biological_process	positive regulation of erythrocyte differentiation
D	0051240	biological_process	positive regulation of multicellular organismal process
D	0051607	biological_process	defense response to virus
D	0060339	biological_process	negative regulation of type I interferon-mediated signaling pathway
D	0070585	biological_process	protein localization to mitochondrion
E	0002682	biological_process	regulation of immune system process
E	0003735	molecular_function	structural constituent of ribosome
E	0005178	molecular_function	integrin binding
E	0005515	molecular_function	protein binding
E	0005576	cellular_component	extracellular region
E	0005634	cellular_component	nucleus
E	0005737	cellular_component	cytoplasm
E	0007229	biological_process	integrin-mediated signaling pathway
E	0009615	biological_process	response to virus
E	0009617	biological_process	response to bacterium
E	0019941	biological_process	modification-dependent protein catabolic process
E	0022626	cellular_component	cytosolic ribosome
E	0030501	biological_process	positive regulation of bone mineralization
E	0031386	molecular_function	protein tag activity
E	0031397	biological_process	negative regulation of protein ubiquitination
E	0032020	biological_process	ISG15-protein conjugation
E	0032461	biological_process	positive regulation of protein oligomerization
E	0032649	biological_process	regulation of type II interferon production
E	0032728	biological_process	positive regulation of interferon-beta production
E	0032729	biological_process	positive regulation of type II interferon production
E	0032733	biological_process	positive regulation of interleukin-10 production
E	0034340	biological_process	response to type I interferon
E	0042742	biological_process	defense response to bacterium
E	0045071	biological_process	negative regulation of viral genome replication
E	0045087	biological_process	innate immune response
E	0045648	biological_process	positive regulation of erythrocyte differentiation
E	0051240	biological_process	positive regulation of multicellular organismal process
E	0051607	biological_process	defense response to virus
E	0060339	biological_process	negative regulation of type I interferon-mediated signaling pathway
E	0070585	biological_process	protein localization to mitochondrion
F	0002682	biological_process	regulation of immune system process
F	0003735	molecular_function	structural constituent of ribosome
F	0005178	molecular_function	integrin binding
F	0005515	molecular_function	protein binding
F	0005576	cellular_component	extracellular region
F	0005634	cellular_component	nucleus
F	0005737	cellular_component	cytoplasm
F	0007229	biological_process	integrin-mediated signaling pathway
F	0009615	biological_process	response to virus
F	0009617	biological_process	response to bacterium
F	0019941	biological_process	modification-dependent protein catabolic process
F	0022626	cellular_component	cytosolic ribosome
F	0030501	biological_process	positive regulation of bone mineralization
F	0031386	molecular_function	protein tag activity
F	0031397	biological_process	negative regulation of protein ubiquitination
F	0032020	biological_process	ISG15-protein conjugation
F	0032461	biological_process	positive regulation of protein oligomerization
F	0032649	biological_process	regulation of type II interferon production
F	0032728	biological_process	positive regulation of interferon-beta production
F	0032729	biological_process	positive regulation of type II interferon production
F	0032733	biological_process	positive regulation of interleukin-10 production
F	0034340	biological_process	response to type I interferon
F	0042742	biological_process	defense response to bacterium
F	0045071	biological_process	negative regulation of viral genome replication
F	0045087	biological_process	innate immune response
F	0045648	biological_process	positive regulation of erythrocyte differentiation
F	0051240	biological_process	positive regulation of multicellular organismal process
F	0051607	biological_process	defense response to virus
F	0060339	biological_process	negative regulation of type I interferon-mediated signaling pathway
F	0070585	biological_process	protein localization to mitochondrion
G	0002682	biological_process	regulation of immune system process
G	0003735	molecular_function	structural constituent of ribosome
G	0005178	molecular_function	integrin binding
G	0005515	molecular_function	protein binding
G	0005576	cellular_component	extracellular region
G	0005634	cellular_component	nucleus
G	0005737	cellular_component	cytoplasm
G	0007229	biological_process	integrin-mediated signaling pathway
G	0009615	biological_process	response to virus
G	0009617	biological_process	response to bacterium
G	0019941	biological_process	modification-dependent protein catabolic process
G	0022626	cellular_component	cytosolic ribosome
G	0030501	biological_process	positive regulation of bone mineralization
G	0031386	molecular_function	protein tag activity
G	0031397	biological_process	negative regulation of protein ubiquitination
G	0032020	biological_process	ISG15-protein conjugation
G	0032461	biological_process	positive regulation of protein oligomerization
G	0032649	biological_process	regulation of type II interferon production
G	0032728	biological_process	positive regulation of interferon-beta production
G	0032729	biological_process	positive regulation of type II interferon production
G	0032733	biological_process	positive regulation of interleukin-10 production
G	0034340	biological_process	response to type I interferon
G	0042742	biological_process	defense response to bacterium
G	0045071	biological_process	negative regulation of viral genome replication
G	0045087	biological_process	innate immune response
G	0045648	biological_process	positive regulation of erythrocyte differentiation
G	0051240	biological_process	positive regulation of multicellular organismal process
G	0051607	biological_process	defense response to virus
G	0060339	biological_process	negative regulation of type I interferon-mediated signaling pathway
G	0070585	biological_process	protein localization to mitochondrion
H	0002682	biological_process	regulation of immune system process
H	0003735	molecular_function	structural constituent of ribosome
H	0005178	molecular_function	integrin binding
H	0005515	molecular_function	protein binding
H	0005576	cellular_component	extracellular region
H	0005634	cellular_component	nucleus
H	0005737	cellular_component	cytoplasm
H	0007229	biological_process	integrin-mediated signaling pathway
H	0009615	biological_process	response to virus
H	0009617	biological_process	response to bacterium
H	0019941	biological_process	modification-dependent protein catabolic process
H	0022626	cellular_component	cytosolic ribosome
H	0030501	biological_process	positive regulation of bone mineralization
H	0031386	molecular_function	protein tag activity
H	0031397	biological_process	negative regulation of protein ubiquitination
H	0032020	biological_process	ISG15-protein conjugation
H	0032461	biological_process	positive regulation of protein oligomerization
H	0032649	biological_process	regulation of type II interferon production
H	0032728	biological_process	positive regulation of interferon-beta production
H	0032729	biological_process	positive regulation of type II interferon production
H	0032733	biological_process	positive regulation of interleukin-10 production
H	0034340	biological_process	response to type I interferon
H	0042742	biological_process	defense response to bacterium
H	0045071	biological_process	negative regulation of viral genome replication
H	0045087	biological_process	innate immune response
H	0045648	biological_process	positive regulation of erythrocyte differentiation
H	0051240	biological_process	positive regulation of multicellular organismal process
H	0051607	biological_process	defense response to virus
H	0060339	biological_process	negative regulation of type I interferon-mediated signaling pathway
H	0070585	biological_process	protein localization to mitochondrion
I	0002682	biological_process	regulation of immune system process
I	0003735	molecular_function	structural constituent of ribosome
I	0005178	molecular_function	integrin binding
I	0005515	molecular_function	protein binding
I	0005576	cellular_component	extracellular region
I	0005634	cellular_component	nucleus
I	0005737	cellular_component	cytoplasm
I	0007229	biological_process	integrin-mediated signaling pathway
I	0009615	biological_process	response to virus
I	0009617	biological_process	response to bacterium
I	0019941	biological_process	modification-dependent protein catabolic process
I	0022626	cellular_component	cytosolic ribosome
I	0030501	biological_process	positive regulation of bone mineralization
I	0031386	molecular_function	protein tag activity
I	0031397	biological_process	negative regulation of protein ubiquitination
I	0032020	biological_process	ISG15-protein conjugation
I	0032461	biological_process	positive regulation of protein oligomerization
I	0032649	biological_process	regulation of type II interferon production
I	0032728	biological_process	positive regulation of interferon-beta production
I	0032729	biological_process	positive regulation of type II interferon production
I	0032733	biological_process	positive regulation of interleukin-10 production
I	0034340	biological_process	response to type I interferon
I	0042742	biological_process	defense response to bacterium
I	0045071	biological_process	negative regulation of viral genome replication
I	0045087	biological_process	innate immune response
I	0045648	biological_process	positive regulation of erythrocyte differentiation
I	0051240	biological_process	positive regulation of multicellular organismal process
I	0051607	biological_process	defense response to virus
I	0060339	biological_process	negative regulation of type I interferon-mediated signaling pathway
I	0070585	biological_process	protein localization to mitochondrion
J	0002682	biological_process	regulation of immune system process
J	0003735	molecular_function	structural constituent of ribosome
J	0005178	molecular_function	integrin binding
J	0005515	molecular_function	protein binding
J	0005576	cellular_component	extracellular region
J	0005634	cellular_component	nucleus
J	0005737	cellular_component	cytoplasm
J	0007229	biological_process	integrin-mediated signaling pathway
J	0009615	biological_process	response to virus
J	0009617	biological_process	response to bacterium
J	0019941	biological_process	modification-dependent protein catabolic process
J	0022626	cellular_component	cytosolic ribosome
J	0030501	biological_process	positive regulation of bone mineralization
J	0031386	molecular_function	protein tag activity
J	0031397	biological_process	negative regulation of protein ubiquitination
J	0032020	biological_process	ISG15-protein conjugation
J	0032461	biological_process	positive regulation of protein oligomerization
J	0032649	biological_process	regulation of type II interferon production
J	0032728	biological_process	positive regulation of interferon-beta production
J	0032729	biological_process	positive regulation of type II interferon production
J	0032733	biological_process	positive regulation of interleukin-10 production
J	0034340	biological_process	response to type I interferon
J	0042742	biological_process	defense response to bacterium
J	0045071	biological_process	negative regulation of viral genome replication
J	0045087	biological_process	innate immune response
J	0045648	biological_process	positive regulation of erythrocyte differentiation
J	0051240	biological_process	positive regulation of multicellular organismal process
J	0051607	biological_process	defense response to virus
J	0060339	biological_process	negative regulation of type I interferon-mediated signaling pathway
J	0070585	biological_process	protein localization to mitochondrion

Functional Information from PDB Data

site_id	AC1
Number of Residues	5
Details	binding site for residue SO4 A 201

Chain	Residue
A	HIS47
A	GLN48
A	SER66
A	SER67
A	THR68

---

site_id	AC2
Number of Residues	6
Details	binding site for residue GOL A 202

Chain	Residue
B	GLY136
B	GLU137
A	GLU95
A	HOH309
B	ALA40
B	PHE41

---

site_id	AC3
Number of Residues	5
Details	binding site for residue GOL A 203

Chain	Residue
A	LYS29
A	LEU52
A	GLN53
A	ASP54
B	GLU130

---

site_id	AC4
Number of Residues	5
Details	binding site for residue SO4 B 201

Chain	Residue
B	HIS47
B	GLN48
B	SER66
B	SER67
B	THR68

---

site_id	AC5
Number of Residues	4
Details	binding site for residue GOL B 202

Chain	Residue
B	VAL73
B	GLN74
B	ASN75
B	SER76

---

site_id	AC6
Number of Residues	4
Details	binding site for residue GOL B 203

Chain	Residue
A	ARG88
B	GLY11
B	ASN13
E	ASN21

---

site_id	AC7
Number of Residues	4
Details	binding site for residue GOL B 204

Chain	Residue
B	GLU95
C	PRO39
C	ALA40
C	GLY136

---

site_id	AC8
Number of Residues	8
Details	binding site for residue SO4 C 201

Chain	Residue
C	HIS47
C	GLN48
C	SER67
C	THR68
C	HOH304
C	HOH310
I	ARG44
I	VAL72

---

site_id	AC9
Number of Residues	4
Details	binding site for residue SO4 D 201

Chain	Residue
D	LYS8
D	GLN119
D	ARG151
D	ARG153

---

site_id	AD1
Number of Residues	9
Details	binding site for residue SO4 D 202

Chain	Residue
A	GLU125
D	THR57
D	SER59
D	SER60
D	HOH305
D	HOH312
D	HOH314
F	ARG151
F	LEU152

---

site_id	AD2
Number of Residues	6
Details	binding site for residue SO4 D 203

Chain	Residue
A	HIS149
A	HOH310
A	HOH318
D	THR20
D	ASN21
D	SER22

---

site_id	AD3
Number of Residues	5
Details	binding site for residue SO4 D 204

Chain	Residue
D	HIS47
D	GLN48
D	SER67
D	THR68
D	ARG153

---

site_id	AD4
Number of Residues	5
Details	binding site for residue GOL D 205

Chain	Residue
D	ALA33
D	ALA40
D	PHE41
D	GLY136
H	GLU95

---

site_id	AD5
Number of Residues	4
Details	binding site for residue SO4 E 201

Chain	Residue
A	ARG88
A	HIS90
E	PRO65
E	SER66

---

site_id	AD6
Number of Residues	7
Details	binding site for residue SO4 E 202

Chain	Residue
A	HIS116
A	GLN119
A	ARG153
E	SER60
E	HOH301
E	HOH307
G	ARG153

---

site_id	AD7
Number of Residues	4
Details	binding site for residue SO4 E 203

Chain	Residue
E	HIS47
E	GLN48
E	SER67
E	THR68

---

site_id	AD8
Number of Residues	6
Details	binding site for residue SO4 G 201

Chain	Residue
A	ARG153
G	SER60
G	HIS116
G	GLN119
G	ARG153
G	HOH319

---

site_id	AD9
Number of Residues	3
Details	binding site for residue SO4 G 202

Chain	Residue
G	HIS47
G	GLN48
G	THR68

---

site_id	AE1
Number of Residues	7
Details	binding site for residue GOL G 203

Chain	Residue
G	GLU95
G	HOH318
A	ALA40
A	PHE41
A	GLY136
A	GLU137
G	PRO79

---

site_id	AE2
Number of Residues	6
Details	binding site for residue GOL G 204

Chain	Residue
A	ARG127
A	GLU130
G	SER76
G	GLU78
G	PRO79
G	HOH315

---

site_id	AE3
Number of Residues	4
Details	binding site for residue SO4 H 201

Chain	Residue
H	HIS47
H	GLN48
H	SER67
H	THR68

---

site_id	AE4
Number of Residues	4
Details	binding site for residue GOL I 201

Chain	Residue
I	HIS47
I	GLN48
I	SER67
I	THR68

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	666
Details	Domain: {"description":"Ubiquitin-like 1","evidences":[{"source":"PROSITE-ProRule","id":"PRU00214","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	312
Details	Domain: {"description":"Ubiquitin-like 2","evidences":[{"source":"PROSITE-ProRule","id":"PRU00214","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	16
Details	Region: {"description":"Involved in the ligation of specific target proteins","evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	20
Details	Motif: {"description":"LRLRGG","evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	8
Details	Site: {"description":"Interacts with activating enzyme","evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	20
Details	Modified residue: {"description":"S-nitrosocysteine","evidences":[{"source":"PubMed","id":"18606809","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	8
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)","evidences":[{"source":"PROSITE-ProRule","id":"PRU00214","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---