5CDN

2.8A structure of etoposide with S.aureus DNA gyrase and DNA

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003677	molecular_function	DNA binding
A	0003918	molecular_function	DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity
A	0005524	molecular_function	ATP binding
A	0006259	biological_process	DNA metabolic process
A	0006265	biological_process	DNA topological change
B	0003677	molecular_function	DNA binding
B	0003918	molecular_function	DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity
B	0005524	molecular_function	ATP binding
B	0006265	biological_process	DNA topological change
C	0003677	molecular_function	DNA binding
C	0003918	molecular_function	DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity
C	0005524	molecular_function	ATP binding
C	0006259	biological_process	DNA metabolic process
C	0006265	biological_process	DNA topological change
D	0003677	molecular_function	DNA binding
D	0003918	molecular_function	DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity
D	0005524	molecular_function	ATP binding
D	0006265	biological_process	DNA topological change
R	0003677	molecular_function	DNA binding
R	0003918	molecular_function	DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity
R	0005524	molecular_function	ATP binding
R	0006259	biological_process	DNA metabolic process
R	0006265	biological_process	DNA topological change
S	0003677	molecular_function	DNA binding
S	0003918	molecular_function	DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity
S	0005524	molecular_function	ATP binding
S	0006265	biological_process	DNA topological change
T	0003677	molecular_function	DNA binding
T	0003918	molecular_function	DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity
T	0005524	molecular_function	ATP binding
T	0006259	biological_process	DNA metabolic process
T	0006265	biological_process	DNA topological change
U	0003677	molecular_function	DNA binding
U	0003918	molecular_function	DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity
U	0005524	molecular_function	ATP binding
U	0006265	biological_process	DNA topological change

Functional Information from PDB Data

site_id	AC1
Number of Residues	6
Details	binding site for residue SO4 A 501

Chain	Residue
A	PRO44
A	ARG47
A	ARG48
A	ILE147
A	GLU156
A	SER158

---

site_id	AC2
Number of Residues	7
Details	binding site for residue MN B 1001

Chain	Residue
B	HOH1102
B	HOH1106
B	HOH1109
B	HOH1123
B	GLU435
B	ASP508
B	ASP510

---

site_id	AC3
Number of Residues	4
Details	binding site for residue GOL C 501

Chain	Residue
C	MET113
C	GLY115
C	VAL268
C	ASN269

---

site_id	AC4
Number of Residues	4
Details	binding site for residue NA C 502

Chain	Residue
C	TYR322
C	LYS323
C	THR325
C	GLN328

---

site_id	AC5
Number of Residues	2
Details	binding site for residue GOL C 503

Chain	Residue
C	ARG377
C	ARG432

---

site_id	AC6
Number of Residues	7
Details	binding site for residue MN D 1001

Chain	Residue
D	GLU435
D	ASP508
D	ASP510
D	HOH1103
D	HOH1108
D	HOH1111
E	HOH204

---

site_id	AC7
Number of Residues	5
Details	binding site for residue GOL E 101

Chain	Residue
E	DC4
E	DG5
E	DT6
E	DA7
O	DT2014

---

site_id	AC8
Number of Residues	11
Details	binding site for residue EVP G 2101

Chain	Residue
A	SER84
A	GLU88
B	GLY436
B	ASP437
B	ARG458
B	GLY459
F	DC8
G	DC2012
G	DG2013
O	DG2009
O	DG2010

---

site_id	AC9
Number of Residues	10
Details	binding site for residue EVP O 2101

Chain	Residue
A	PTR123
C	SER84
D	GLY436
D	ASP437
D	ARG458
D	GLY459
E	DC8
G	DG2009
O	DC2012
O	DG2013

---

site_id	AD1
Number of Residues	7
Details	binding site for residue MN S 6001

Chain	Residue
S	GLU435
S	ASP508
S	ASP510
S	HOH6102
S	HOH6108
W	HOH102
W	HOH106

---

site_id	AD2
Number of Residues	5
Details	binding site for residue GOL T 501

Chain	Residue
T	VAL31
T	PRO44
T	ARG47
T	ILE147
T	GLU156

---

site_id	AD3
Number of Residues	7
Details	binding site for residue MN U 1001

Chain	Residue
U	GLU435
U	ASP508
U	ASP510
U	HOH1102
U	HOH1105
V	HOH102
V	HOH104

---

site_id	AD4
Number of Residues	11
Details	binding site for residue EVP N 2101

Chain	Residue
N	DC2012
N	DG2013
P	DG2009
P	DG2010
R	SER84
R	GLU88
S	GLY436
S	ASP437
S	ARG458
S	GLY459
W	DC8

---

site_id	AD5
Number of Residues	12
Details	binding site for residue EVP P 2101

Chain	Residue
N	DG2009
N	DG2010
P	DC2012
P	DG2013
R	PTR123
T	SER84
T	GLU88
U	GLY436
U	ASP437
U	ARG458
U	GLY459
V	DC8

---

Functional Information from PROSITE/UniProt

site_id	PS00177
Number of Residues	9
Details	TOPOISOMERASE_II DNA topoisomerase II signature. LVEGDSAGG

Chain	Residue	Details
B	LEU433-GLY441

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	Active site: {"description":"O-(5'-phospho-DNA)-tyrosine intermediate","evidences":[{"source":"HAMAP-Rule","id":"MF_01897","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	456
Details	Domain: {"description":"Toprim","evidences":[{"source":"HAMAP-Rule","id":"MF_01898","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	12
Details	Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_01898","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	8
Details	Site: {"description":"Interaction with DNA","evidences":[{"source":"HAMAP-Rule","id":"MF_01898","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---