5C4H

Crystal structure of Staphylococcal nuclease variant Delta+PHS I92H at cryogenic temperature

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003676	molecular_function	nucleic acid binding
A	0004518	molecular_function	nuclease activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	7
Details	binding site for residue CA A 201

Chain	Residue
A	ASP21
A	ASP40
A	THR41
A	GLU43
A	THP202
A	HOH308
A	HOH335

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site_id	AC2
Number of Residues	22
Details	binding site for residue THP A 202

Chain	Residue
A	LYS84
A	TYR85
A	ARG87
A	LEU89
A	TYR113
A	TYR115
A	LYS127
A	CA201
A	HOH308
A	HOH309
A	HOH316
A	HOH318
A	HOH325
A	HOH341
A	HOH344
A	HOH360
A	HOH365
A	HOH369
A	HOH372
A	HOH373
A	ARG35
A	ASP40

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Functional Information from PROSITE/UniProt

site_id	PS01123
Number of Residues	25
Details	TNASE_1 Thermonuclease family signature 1. DGDTVkLmykgqpmtf.RLllVDtPE

Chain	Residue	Details
A	ASP19-GLU43

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	16
Details	Compositional bias: {"description":"Basic and acidic residues","evidences":[{"source":"SAM","id":"MobiDB-lite","evidenceCode":"ECO:0000256"}]}

Chain

Residue

Details

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site_id	SWS_FT_FI2
Number of Residues	3
Details	Active site: {"evidences":[{"source":"PubMed","id":"288045","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

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site_id	SWS_FT_FI3
Number of Residues	3
Details	Binding site: {}

Chain

Residue

Details

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Catalytic Information from CSA

site_id	MCSA1
Number of Residues	6
Details	M-CSA 165

Chain	Residue	Details
A	ASP21	metal ligand
A	ARG35	electrostatic stabiliser, hydrogen bond donor
A	ASP40	metal ligand
A	THR41	metal ligand
A	GLU43	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
A	ARG87	electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor

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