Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

5BV2

Crystal structure of E. coli HPII catalase variant

Functional Information from GO Data

Chain	GOid	namespace	contents
P	0004096	molecular_function	catalase activity
P	0004601	molecular_function	peroxidase activity
P	0005506	molecular_function	iron ion binding
P	0005737	cellular_component	cytoplasm
P	0005829	cellular_component	cytosol
P	0006972	biological_process	hyperosmotic response
P	0006974	biological_process	DNA damage response
P	0006979	biological_process	response to oxidative stress
P	0020037	molecular_function	heme binding
P	0042744	biological_process	hydrogen peroxide catabolic process
P	0042802	molecular_function	identical protein binding
P	0046872	molecular_function	metal ion binding
P	0098869	biological_process	cellular oxidant detoxification
Q	0004096	molecular_function	catalase activity
Q	0004601	molecular_function	peroxidase activity
Q	0005506	molecular_function	iron ion binding
Q	0005737	cellular_component	cytoplasm
Q	0005829	cellular_component	cytosol
Q	0006972	biological_process	hyperosmotic response
Q	0006974	biological_process	DNA damage response
Q	0006979	biological_process	response to oxidative stress
Q	0020037	molecular_function	heme binding
Q	0042744	biological_process	hydrogen peroxide catabolic process
Q	0042802	molecular_function	identical protein binding
Q	0046872	molecular_function	metal ion binding
Q	0098869	biological_process	cellular oxidant detoxification
R	0004096	molecular_function	catalase activity
R	0004601	molecular_function	peroxidase activity
R	0005506	molecular_function	iron ion binding
R	0005737	cellular_component	cytoplasm
R	0005829	cellular_component	cytosol
R	0006972	biological_process	hyperosmotic response
R	0006974	biological_process	DNA damage response
R	0006979	biological_process	response to oxidative stress
R	0020037	molecular_function	heme binding
R	0042744	biological_process	hydrogen peroxide catabolic process
R	0042802	molecular_function	identical protein binding
R	0046872	molecular_function	metal ion binding
R	0098869	biological_process	cellular oxidant detoxification
S	0004096	molecular_function	catalase activity
S	0004601	molecular_function	peroxidase activity
S	0005506	molecular_function	iron ion binding
S	0005737	cellular_component	cytoplasm
S	0005829	cellular_component	cytosol
S	0006972	biological_process	hyperosmotic response
S	0006974	biological_process	DNA damage response
S	0006979	biological_process	response to oxidative stress
S	0020037	molecular_function	heme binding
S	0042744	biological_process	hydrogen peroxide catabolic process
S	0042802	molecular_function	identical protein binding
S	0046872	molecular_function	metal ion binding
S	0098869	biological_process	cellular oxidant detoxification

Functional Information from PDB Data

site_id	AC1
Number of Residues	22
Details	binding site for residue HDD P 801

Chain	Residue
P	ARG125
P	PHE214
P	ILE274
P	HIS275
P	PHE391
P	LEU407
P	ARG411
P	SER414
P	TYR415
P	THR418
P	GLN419
P	ILE126
P	GOL802
P	HOH1025
P	HOH1117
P	VAL127
P	HIS128
P	ARG165
P	GLY184
P	VAL199
P	GLY200
P	ASN201

site_id	AC2
Number of Residues	7
Details	binding site for residue GOL P 802

Chain	Residue
P	VAL127
P	HIS128
P	VAL169
P	ASN201
P	PHE206
P	PHE214
P	HDD801

site_id	AC3
Number of Residues	7
Details	binding site for residue EDO P 803

Chain	Residue
P	GLY98
P	ASP99
P	HOH1358
R	HOH1343
S	GLY491
S	ASN492
S	HOH1523

site_id	AC4
Number of Residues	7
Details	binding site for residue EDO P 804

Chain	Residue
P	THR516
P	ALA592
P	HOH921
R	ASP712
R	GLN713
R	HOH1649
R	HOH1974

site_id	AC5
Number of Residues	5
Details	binding site for residue EDO P 805

Chain	Residue
P	PRO517
P	PHE518
P	ARG521
P	HOH940
P	HOH1062

site_id	AC6
Number of Residues	5
Details	binding site for residue EDO P 806

Chain	Residue
P	LYS709
P	PRO752
P	HOH1247
S	ASN682
S	THR707

site_id	AC7
Number of Residues	5
Details	binding site for residue EDO P 807

Chain	Residue
P	TYR634
P	SER635
P	ASP674
P	ASN678
P	HOH1251

site_id	AC8
Number of Residues	6
Details	binding site for residue EDO P 808

Chain	Residue
P	ASP155
P	ASN157
P	LYS158
P	HOH1095
P	HOH1335
P	HOH1524

site_id	AC9
Number of Residues	8
Details	binding site for residue EDO P 809

Chain	Residue
P	VAL179
P	GLN231
P	GLN233
P	THR311
P	HOH936
P	HOH1102
P	HOH1145
S	GLY312

site_id	AD1
Number of Residues	8
Details	binding site for residue EDO P 810

Chain	Residue
P	PRO594
P	ASP595
P	GLY596
P	ALA737
P	HOH961
P	HOH1123
P	HOH1636
P	HOH1784

site_id	AD2
Number of Residues	8
Details	binding site for residue EDO P 811

Chain	Residue
P	PHE347
P	PHE349
P	PHE351
P	SER500
P	HOH1032
P	HOH1073
P	HOH1322
S	HOH1894

site_id	AD3
Number of Residues	5
Details	binding site for residue PGE P 812

Chain	Residue
P	SER613
P	LEU616
P	LEU648
P	HOH1788
R	ASN157

site_id	AD4
Number of Residues	9
Details	binding site for residue GOL P 813

Chain	Residue
P	HOH1325
P	HOH1361
R	HOH957
P	ARG121
P	ALA173
P	PHE382
P	GLU387
P	HOH925
P	HOH938

site_id	AD5
Number of Residues	12
Details	binding site for residue PEG P 814

Chain	Residue
P	ASP305
P	LYS309
P	TYR683
P	LYS690
P	ALA753
P	HOH919
P	HOH950
S	ARG313
S	ASP680
S	TYR683
S	HOH934
S	HOH1356

site_id	AD6
Number of Residues	9
Details	binding site for residue PEG P 815

Chain	Residue
P	PHE349
P	LEU364
P	ASN580
P	HOH933
P	HOH1028
P	HOH1273
S	GLY581
S	LEU582
S	HOH1915

site_id	AD7
Number of Residues	8
Details	binding site for residue PEG P 816

Chain	Residue
P	LYS142
P	ASP146
P	LEU340
P	GLN368
P	VAL370
P	HOH993
P	HOH1220
P	HOH1523

site_id	AD8
Number of Residues	13
Details	binding site for residue PEG P 817

Chain	Residue
P	ARG264
P	LEU302
P	GLU306
P	PHE317
P	ARG601
P	LEU660
P	THR661
P	VAL662
P	ASP663
P	LYS693
P	HIS739
P	ARG740
P	HOH1069

site_id	AD9
Number of Residues	7
Details	binding site for residue SO4 P 818

Chain	Residue
P	ARG72
P	LYS73
P	GLY74
P	TYR78
P	HOH914
P	HOH1646
R	HIS441

site_id	AE1
Number of Residues	22
Details	binding site for residue HDD Q 801

Chain	Residue
Q	ARG125
Q	ILE126
Q	VAL127
Q	HIS128
Q	ARG165
Q	GLY184
Q	VAL199
Q	GLY200
Q	ASN201
Q	PHE214
Q	ILE274
Q	HIS275
Q	PHE391
Q	LEU407
Q	ARG411
Q	SER414
Q	TYR415
Q	THR418
Q	GLN419
Q	GOL802
Q	HOH1004
Q	HOH1123

site_id	AE2
Number of Residues	7
Details	binding site for residue GOL Q 802

Chain	Residue
Q	VAL127
Q	HIS128
Q	VAL169
Q	ASN201
Q	PHE206
Q	PHE214
Q	HDD801

site_id	AE3
Number of Residues	8
Details	binding site for residue EDO Q 803

Chain	Residue
Q	GLU224
Q	ASP237
Q	ARG536
Q	PEG822
Q	HOH904
Q	HOH1118
R	HOH1132
R	HOH1589

site_id	AE4
Number of Residues	6
Details	binding site for residue EDO Q 804

Chain	Residue
Q	GLY98
Q	ASP99
Q	HOH1132
R	GLY491
R	ASN492
R	HOH1376

site_id	AE5
Number of Residues	5
Details	binding site for residue EDO Q 805

Chain	Residue
Q	ARG61
Q	ARG61
Q	ASN66
Q	HOH1042
Q	HOH1399

site_id	AE6
Number of Residues	2
Details	binding site for residue EDO Q 806

Chain	Residue
Q	GLU69
Q	HOH946

site_id	AE7
Number of Residues	5
Details	binding site for residue EDO Q 807

Chain	Residue
Q	ARG542
Q	HOH1066
R	HIS12
R	GLN13
R	GLU32

site_id	AE8
Number of Residues	9
Details	binding site for residue EDO Q 808

Chain	Residue
Q	VAL179
Q	GLN231
Q	TRP304
Q	THR311
Q	HOH1034
Q	HOH1315
R	GLY312
R	HOH1035
R	HOH1268

site_id	AE9
Number of Residues	7
Details	binding site for residue EDO Q 809

Chain	Residue
Q	LEU620
Q	LYS624
Q	VAL628
Q	ALA630
Q	HOH935
Q	HOH1054
Q	HOH1639

site_id	AF1
Number of Residues	8
Details	binding site for residue EDO Q 810

Chain	Residue
Q	ARG471
Q	GLU472
Q	THR473
Q	HOH965
Q	HOH1238
Q	HOH1616
S	ALA79
S	ARG87

site_id	AF2
Number of Residues	7
Details	binding site for residue EDO Q 811

Chain	Residue
Q	GLN139
Q	PRO140
Q	TYR141
Q	ASP155
Q	ASN157
Q	HOH1878
Q	HOH1972

site_id	AF3
Number of Residues	6
Details	binding site for residue EDO Q 812

Chain	Residue
Q	ASP578
Q	HOH1023
Q	HOH1198
Q	HOH1389
Q	HOH1414
R	ASP350

site_id	AF4
Number of Residues	5
Details	binding site for residue EDO Q 813

Chain	Residue
Q	SER145
Q	ASN281
Q	ALA282
Q	SER283
Q	GOL821

site_id	AF5
Number of Residues	4
Details	binding site for residue EDO Q 814

Chain	Residue
Q	HIS441
S	ARG72
S	LYS73
S	GLY74

site_id	AF6
Number of Residues	4
Details	binding site for residue EDO Q 815

Chain	Residue
Q	ARG37
Q	ALA39
Q	HOH964
Q	HOH1255

site_id	AF7
Number of Residues	9
Details	binding site for residue EDO Q 816

Chain	Residue
Q	SER35
Q	ALA47
Q	GLN48
Q	PRO49
Q	HOH911
Q	HOH976
Q	HOH1236
R	VAL535
R	HOH978

site_id	AF8
Number of Residues	1
Details	binding site for residue EDO Q 817

Chain	Residue
Q	PHE347

site_id	AF9
Number of Residues	3
Details	binding site for residue EDO Q 818

Chain	Residue
Q	THR707
Q	HOH1049
Q	HOH1464

site_id	AG1
Number of Residues	4
Details	binding site for residue EDO Q 819

Chain	Residue
Q	LYS626
Q	ASP730
Q	LEU733
Q	THR734

site_id	AG2
Number of Residues	6
Details	binding site for residue GOL Q 820

Chain	Residue
Q	THR574
Q	PRO575
Q	PRO577
Q	HOH1015
Q	HOH1488
Q	HOH1605

site_id	AG3
Number of Residues	4
Details	binding site for residue GOL Q 821

Chain	Residue
Q	ALA282
Q	PRO477
Q	EDO813
Q	HOH1254

site_id	AG4
Number of Residues	11
Details	binding site for residue PEG Q 822

Chain	Residue
Q	ILE229
Q	GLN233
Q	ALA235
Q	HIS236
Q	EDO803
Q	HOH904
Q	HOH1118
Q	HOH1392
Q	HOH1769
R	ARG320
R	HOH1137

site_id	AG5
Number of Residues	5
Details	binding site for residue PEG Q 823

Chain	Residue
Q	ASN77
Q	TYR78
Q	ALA79
Q	ARG87
Q	HOH1499

site_id	AG6
Number of Residues	8
Details	binding site for residue PEG Q 824

Chain	Residue
Q	LYS299
Q	GLU362
Q	SER587
Q	ILE593
Q	ASP595
Q	HOH931
Q	HOH1110
Q	HOH1381

site_id	AG7
Number of Residues	5
Details	binding site for residue PEG Q 825

Chain	Residue
Q	LYS142
Q	GLN368
Q	ARG369
Q	HOH923
Q	HOH1446

site_id	AG8
Number of Residues	23
Details	binding site for residue HDD R 801

Chain	Residue
R	ARG125
R	ILE126
R	VAL127
R	HIS128
R	ARG165
R	GLY184
R	VAL199
R	GLY200
R	ASN201
R	PHE214
R	ILE274
R	HIS275
R	PHE391
R	LEU407
R	ARG411
R	SER414
R	TYR415
R	THR418
R	GLN419
R	GOL802
R	HOH972
R	HOH1136
R	HOH1203

site_id	AG9
Number of Residues	8
Details	binding site for residue GOL R 802

Chain	Residue
R	VAL127
R	HIS128
R	VAL169
R	ASN201
R	PHE206
R	PHE214
R	HDD801
R	HOH1052

site_id	AH1
Number of Residues	7
Details	binding site for residue EDO R 803

Chain	Residue
R	ARG521
R	ILE745
R	PRO746
R	ILE748
R	ASP749
R	PGE807
R	HOH1112

site_id	AH2
Number of Residues	3
Details	binding site for residue EDO R 804

Chain	Residue
R	HOH936
R	HOH1023
R	HOH1389

site_id	AH3
Number of Residues	7
Details	binding site for residue EDO R 805

Chain	Residue
Q	GLY312
R	VAL179
R	GLN231
R	THR311
R	HOH906
R	HOH1188
R	HOH1196

site_id	AH4
Number of Residues	5
Details	binding site for residue EDO R 806

Chain	Residue
R	SER613
R	ALA614
R	LEU617
R	ASP644
R	ASP644

site_id	AH5
Number of Residues	14
Details	binding site for residue PGE R 807

Chain	Residue
R	VAL303
R	ASP305
R	ARG521
R	HIS522
R	LYS690
R	LEU692
R	ILE745
R	ILE748
R	EDO803
R	HOH919
R	HOH935
R	HOH1193
R	HOH1265
R	HOH1686

site_id	AH6
Number of Residues	10
Details	binding site for residue PGE R 808

Chain	Residue
R	LYS149
R	ALA282
R	PRO475
R	GLY476
R	PRO477
R	GLU483
R	GLN486
R	HOH1093
R	HOH1332
R	HOH1594

site_id	AH7
Number of Residues	11
Details	binding site for residue GOL R 809

Chain	Residue
Q	LYS583
R	PHE347
R	PHE349
R	PHE351
R	ASP352
R	SER500
R	HOH925
R	HOH1067
R	HOH1149
R	HOH1320
R	HOH1569

site_id	AH8
Number of Residues	9
Details	binding site for residue GOL R 810

Chain	Residue
P	HOH1257
Q	GLY491
Q	ASN492
Q	GLU496
Q	HOH1374
R	GLY98
R	ASP99
R	HOH915
R	HOH977

site_id	AH9
Number of Residues	7
Details	binding site for residue MG R 811

Chain	Residue
R	GLU63
R	HOH922
R	HOH1058
R	HOH1808
R	HOH1871
R	HOH1884
R	HOH1961

site_id	AI1
Number of Residues	9
Details	binding site for residue PG4 R 812

Chain	Residue
P	ASP644
P	GLY645
P	THR646
R	SER143
R	SER145
R	SER154
R	LYS478
R	HOH938
R	HOH1363

site_id	AI2
Number of Residues	23
Details	binding site for residue HDD S 801

Chain	Residue
S	ARG125
S	ILE126
S	VAL127
S	HIS128
S	ARG165
S	GLY184
S	VAL199
S	GLY200
S	ASN201
S	PHE214
S	ILE274
S	HIS275
S	PHE391
S	LEU407
S	ARG411
S	SER414
S	TYR415
S	THR418
S	GLN419
S	GOL802
S	HOH1021
S	HOH1160
S	HOH1181

site_id	AI3
Number of Residues	7
Details	binding site for residue GOL S 802

Chain	Residue
S	VAL127
S	HIS128
S	VAL169
S	ASN201
S	PHE206
S	PHE214
S	HDD801

site_id	AI4
Number of Residues	6
Details	binding site for residue EDO S 803

Chain	Residue
Q	ALA79
Q	ARG87
S	ARG471
S	GLU472
S	THR473
S	HOH964

site_id	AI5
Number of Residues	8
Details	binding site for residue EDO S 804

Chain	Residue
S	VAL303
S	ASP305
S	LYS690
S	HIS691
S	ILE748
S	EDO805
S	HOH994
S	HOH1255

site_id	AI6
Number of Residues	9
Details	binding site for residue EDO S 805

Chain	Residue
S	ARG521
S	HIS522
S	ILE745
S	ILE748
S	EDO804
S	HOH910
S	HOH1038
S	HOH1255
S	HOH1661

site_id	AI7
Number of Residues	3
Details	binding site for residue EDO S 806

Chain	Residue
S	ALA630
S	HOH1482
S	HOH1615

site_id	AI8
Number of Residues	4
Details	binding site for residue EDO S 807

Chain	Residue
S	TYR485
S	GLN486
S	HOH1192
S	HOH1669

site_id	AI9
Number of Residues	8
Details	binding site for residue EDO S 808

Chain	Residue
Q	VAL71
Q	LYS73
S	GLU430
S	ARG435
S	TYR440
S	MET451
S	HOH947
S	HOH1289

site_id	AJ1
Number of Residues	5
Details	binding site for residue EDO S 809

Chain	Residue
S	ASN8
S	PRO9
S	HIS10
S	GLN11
S	HIS12

site_id	AJ2
Number of Residues	5
Details	binding site for residue EDO S 810

Chain	Residue
P	PRO537
S	SER35
S	ALA47
S	PRO49
S	HOH1581

site_id	AJ3
Number of Residues	2
Details	binding site for residue EDO S 811

Chain	Residue
S	PRO517
S	HOH1022

site_id	AJ4
Number of Residues	5
Details	binding site for residue GOL S 812

Chain	Residue
S	ASP609
S	TYR634
S	ARG636
S	HOH922
S	HOH1040

site_id	AJ5
Number of Residues	10
Details	binding site for residue GOL S 813

Chain	Residue
P	GLY491
P	ASN492
P	GLU496
P	HOH1378
S	GLY98
S	ASP99
S	HOH911
S	HOH938
S	HOH954
S	HOH1891

site_id	AJ6
Number of Residues	8
Details	binding site for residue SO4 S 814

Chain	Residue
P	ARG542
S	HIS12
S	PRO31
S	GLU32
S	HOH926
S	HOH1140
S	HOH1214
S	HOH1311

site_id	AJ7
Number of Residues	6
Details	binding site for residue MG S 815

Chain	Residue
S	HOH1448
S	HOH1725
S	HOH1732
S	HOH1973
S	HOH2101
S	HOH2108

site_id	AJ8
Number of Residues	13
Details	binding site for residue PEG S 816

Chain	Residue
P	ARG320
P	HOH1291
S	TRP227
S	ILE229
S	PRO230
S	GLN233
S	ALA235
S	HIS236
S	HOH907
S	HOH1019
S	HOH1110
S	HOH1507
S	HOH1735

site_id	AJ9
Number of Residues	9
Details	binding site for residue PEG S 817

Chain	Residue
S	LYS149
S	ALA282
S	PRO475
S	GLY476
S	PRO477
S	GLU483
S	HOH997
S	HOH1350
S	HOH1768

Functional Information from PROSITE/UniProt

site_id	PS00437
Number of Residues	9
Details	CATALASE_1 Catalase proximal heme-ligand signature. RLFSYtDTQ

Chain	Residue	Details
P	ARG411-GLN419

site_id	PS00438
Number of Residues	17
Details	CATALASE_2 Catalase proximal active site signature. FdHeripERivHarGSA

Chain	Residue	Details
P	PHE117-ALA133

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	8
Details	ACT_SITE: ACT_SITE => ECO:0000255\|PROSITE-ProRule:PRU10013

Chain	Residue	Details
P	HIS128
P	ASN201
Q	HIS128
Q	ASN201
R	HIS128
R	ASN201
S	HIS128
S	ASN201

site_id	SWS_FT_FI2
Number of Residues	4
Details	BINDING: axial binding residue

Chain	Residue	Details
P	TYR415
Q	TYR415
R	TYR415
S	TYR415

site_id	SWS_FT_FI3
Number of Residues	8
Details	CROSSLNK: 3'-histidyl-3-tyrosine (His-Tyr)

Chain	Residue	Details
P	HIS392
P	TYR415
Q	HIS392
Q	TYR415
R	HIS392
R	TYR415
S	HIS392
S	TYR415

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	3
Details	M-CSA 573

Chain	Residue	Details
P	HIS128	proton shuttle (general acid/base)
P	ASN201	electrostatic stabiliser
P	HIS392	proton shuttle (general acid/base)

site_id	MCSA2
Number of Residues	3
Details	M-CSA 573

Chain	Residue	Details
Q	HIS128	proton shuttle (general acid/base)
Q	ASN201	electrostatic stabiliser
Q	HIS392	proton shuttle (general acid/base)

site_id	MCSA3
Number of Residues	3
Details	M-CSA 573

Chain	Residue	Details
R	HIS128	proton shuttle (general acid/base)
R	ASN201	electrostatic stabiliser
R	HIS392	proton shuttle (general acid/base)

site_id	MCSA4
Number of Residues	3
Details	M-CSA 573

Chain	Residue	Details
S	HIS128	proton shuttle (general acid/base)
S	ASN201	electrostatic stabiliser
S	HIS392	proton shuttle (general acid/base)

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)