5BSF
Crystal structure of Medicago truncatula (delta)1-Pyrroline-5-Carboxylate Reductase (MtP5CR) in complex with NAD+
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000166 | molecular_function | nucleotide binding |
| A | 0004735 | molecular_function | pyrroline-5-carboxylate reductase activity |
| A | 0006561 | biological_process | proline biosynthetic process |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0055129 | biological_process | L-proline biosynthetic process |
| B | 0000166 | molecular_function | nucleotide binding |
| B | 0004735 | molecular_function | pyrroline-5-carboxylate reductase activity |
| B | 0006561 | biological_process | proline biosynthetic process |
| B | 0016491 | molecular_function | oxidoreductase activity |
| B | 0055129 | biological_process | L-proline biosynthetic process |
| C | 0000166 | molecular_function | nucleotide binding |
| C | 0004735 | molecular_function | pyrroline-5-carboxylate reductase activity |
| C | 0006561 | biological_process | proline biosynthetic process |
| C | 0016491 | molecular_function | oxidoreductase activity |
| C | 0055129 | biological_process | L-proline biosynthetic process |
| D | 0000166 | molecular_function | nucleotide binding |
| D | 0004735 | molecular_function | pyrroline-5-carboxylate reductase activity |
| D | 0006561 | biological_process | proline biosynthetic process |
| D | 0016491 | molecular_function | oxidoreductase activity |
| D | 0055129 | biological_process | L-proline biosynthetic process |
| E | 0000166 | molecular_function | nucleotide binding |
| E | 0004735 | molecular_function | pyrroline-5-carboxylate reductase activity |
| E | 0006561 | biological_process | proline biosynthetic process |
| E | 0016491 | molecular_function | oxidoreductase activity |
| E | 0055129 | biological_process | L-proline biosynthetic process |
| F | 0000166 | molecular_function | nucleotide binding |
| F | 0004735 | molecular_function | pyrroline-5-carboxylate reductase activity |
| F | 0006561 | biological_process | proline biosynthetic process |
| F | 0016491 | molecular_function | oxidoreductase activity |
| F | 0055129 | biological_process | L-proline biosynthetic process |
| G | 0000166 | molecular_function | nucleotide binding |
| G | 0004735 | molecular_function | pyrroline-5-carboxylate reductase activity |
| G | 0006561 | biological_process | proline biosynthetic process |
| G | 0016491 | molecular_function | oxidoreductase activity |
| G | 0055129 | biological_process | L-proline biosynthetic process |
| H | 0000166 | molecular_function | nucleotide binding |
| H | 0004735 | molecular_function | pyrroline-5-carboxylate reductase activity |
| H | 0006561 | biological_process | proline biosynthetic process |
| H | 0016491 | molecular_function | oxidoreductase activity |
| H | 0055129 | biological_process | L-proline biosynthetic process |
| I | 0000166 | molecular_function | nucleotide binding |
| I | 0004735 | molecular_function | pyrroline-5-carboxylate reductase activity |
| I | 0006561 | biological_process | proline biosynthetic process |
| I | 0016491 | molecular_function | oxidoreductase activity |
| I | 0055129 | biological_process | L-proline biosynthetic process |
| J | 0000166 | molecular_function | nucleotide binding |
| J | 0004735 | molecular_function | pyrroline-5-carboxylate reductase activity |
| J | 0006561 | biological_process | proline biosynthetic process |
| J | 0016491 | molecular_function | oxidoreductase activity |
| J | 0055129 | biological_process | L-proline biosynthetic process |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 23 |
| Details | binding site for residue NAD A 301 |
| Chain | Residue |
| A | GLY17 |
| A | PRO81 |
| A | LEU83 |
| A | VAL87 |
| A | VAL104 |
| A | ALA105 |
| A | ALA106 |
| A | PRO127 |
| A | MPO302 |
| A | HOH415 |
| A | HOH421 |
| A | GLY19 |
| A | HOH423 |
| A | HOH471 |
| A | HOH480 |
| A | HOH489 |
| A | LYS20 |
| A | MET21 |
| A | HIS45 |
| A | ASN65 |
| A | SER78 |
| A | VAL79 |
| A | LYS80 |
| site_id | AC2 |
| Number of Residues | 12 |
| Details | binding site for residue MPO A 302 |
| Chain | Residue |
| A | PRO81 |
| A | ALA106 |
| A | MET126 |
| A | GLY180 |
| A | NAD301 |
| A | HOH417 |
| A | HOH423 |
| A | HOH530 |
| B | SER238 |
| B | THR242 |
| B | CL303 |
| B | HOH517 |
| site_id | AC3 |
| Number of Residues | 5 |
| Details | binding site for residue CL A 303 |
| Chain | Residue |
| A | VAL236 |
| A | SER238 |
| A | THR243 |
| A | HOH427 |
| B | MPO302 |
| site_id | AC4 |
| Number of Residues | 24 |
| Details | binding site for residue NAD B 301 |
| Chain | Residue |
| B | GLY17 |
| B | GLY19 |
| B | LYS20 |
| B | MET21 |
| B | HIS45 |
| B | SER46 |
| B | ASN65 |
| B | SER78 |
| B | VAL79 |
| B | LYS80 |
| B | PRO81 |
| B | VAL87 |
| B | VAL104 |
| B | ALA105 |
| B | ALA106 |
| B | PRO127 |
| B | THR129 |
| B | MPO302 |
| B | HOH423 |
| B | HOH437 |
| B | HOH454 |
| B | HOH473 |
| B | HOH495 |
| B | HOH498 |
| site_id | AC5 |
| Number of Residues | 12 |
| Details | binding site for residue MPO B 302 |
| Chain | Residue |
| A | SER238 |
| A | THR242 |
| A | THR243 |
| A | CL303 |
| B | LYS80 |
| B | PRO81 |
| B | ALA106 |
| B | MET126 |
| B | GLY180 |
| B | SER181 |
| B | NAD301 |
| B | HOH459 |
| site_id | AC6 |
| Number of Residues | 5 |
| Details | binding site for residue CL B 303 |
| Chain | Residue |
| A | MPO302 |
| B | VAL236 |
| B | SER238 |
| B | THR243 |
| B | HOH431 |
| site_id | AC7 |
| Number of Residues | 25 |
| Details | binding site for residue NAD C 301 |
| Chain | Residue |
| C | MPO302 |
| C | HOH408 |
| C | HOH413 |
| C | HOH430 |
| C | HOH464 |
| C | HOH467 |
| C | HOH473 |
| C | GLY17 |
| C | GLY19 |
| C | LYS20 |
| C | MET21 |
| C | HIS45 |
| C | ASN47 |
| C | ASN65 |
| C | SER78 |
| C | VAL79 |
| C | LYS80 |
| C | PRO81 |
| C | LEU83 |
| C | VAL87 |
| C | VAL104 |
| C | ALA105 |
| C | ALA106 |
| C | PRO127 |
| C | THR129 |
| site_id | AC8 |
| Number of Residues | 9 |
| Details | binding site for residue MPO C 302 |
| Chain | Residue |
| C | PRO81 |
| C | ALA106 |
| C | MET126 |
| C | GLY180 |
| C | NAD301 |
| C | HOH417 |
| D | THR242 |
| D | THR243 |
| D | CL303 |
| site_id | AC9 |
| Number of Residues | 5 |
| Details | binding site for residue CL C 303 |
| Chain | Residue |
| C | SER238 |
| C | THR242 |
| C | THR243 |
| C | HOH436 |
| D | MPO302 |
| site_id | AD1 |
| Number of Residues | 23 |
| Details | binding site for residue NAD D 301 |
| Chain | Residue |
| D | GLY17 |
| D | ALA18 |
| D | GLY19 |
| D | LYS20 |
| D | MET21 |
| D | HIS45 |
| D | ASN65 |
| D | SER78 |
| D | VAL79 |
| D | LYS80 |
| D | PRO81 |
| D | LEU83 |
| D | VAL87 |
| D | VAL104 |
| D | ALA105 |
| D | ALA106 |
| D | PRO127 |
| D | THR129 |
| D | MPO302 |
| D | HOH408 |
| D | HOH428 |
| D | HOH464 |
| D | HOH474 |
| site_id | AD2 |
| Number of Residues | 8 |
| Details | binding site for residue MPO D 302 |
| Chain | Residue |
| C | SER238 |
| C | THR242 |
| C | CL303 |
| D | PRO81 |
| D | MET126 |
| D | GLY180 |
| D | NAD301 |
| D | HOH459 |
| site_id | AD3 |
| Number of Residues | 5 |
| Details | binding site for residue CL D 303 |
| Chain | Residue |
| C | MPO302 |
| D | VAL236 |
| D | SER238 |
| D | THR243 |
| D | HOH415 |
| site_id | AD4 |
| Number of Residues | 23 |
| Details | binding site for residue NAD E 301 |
| Chain | Residue |
| E | GLY17 |
| E | GLY19 |
| E | LYS20 |
| E | MET21 |
| E | HIS45 |
| E | ASN65 |
| E | SER78 |
| E | VAL79 |
| E | LYS80 |
| E | PRO81 |
| E | LEU83 |
| E | VAL87 |
| E | VAL104 |
| E | ALA105 |
| E | ALA106 |
| E | PRO127 |
| E | THR129 |
| E | MPO302 |
| E | HOH418 |
| E | HOH436 |
| E | HOH439 |
| E | HOH497 |
| E | HOH509 |
| site_id | AD5 |
| Number of Residues | 11 |
| Details | binding site for residue MPO E 302 |
| Chain | Residue |
| E | PRO81 |
| E | ALA106 |
| E | MET126 |
| E | THR176 |
| E | GLY180 |
| E | SER181 |
| E | NAD301 |
| F | SER238 |
| F | THR242 |
| F | CL303 |
| F | HOH522 |
| site_id | AD6 |
| Number of Residues | 5 |
| Details | binding site for residue CL E 303 |
| Chain | Residue |
| E | VAL236 |
| E | SER238 |
| E | THR243 |
| E | HOH426 |
| F | MPO302 |
| site_id | AD7 |
| Number of Residues | 26 |
| Details | binding site for residue NAD F 301 |
| Chain | Residue |
| F | GLY17 |
| F | ALA18 |
| F | GLY19 |
| F | LYS20 |
| F | MET21 |
| F | HIS45 |
| F | ASN65 |
| F | SER78 |
| F | VAL79 |
| F | LYS80 |
| F | PRO81 |
| F | LEU83 |
| F | VAL87 |
| F | VAL104 |
| F | ALA105 |
| F | ALA106 |
| F | PRO127 |
| F | THR129 |
| F | MPO302 |
| F | HOH408 |
| F | HOH416 |
| F | HOH419 |
| F | HOH478 |
| F | HOH490 |
| F | HOH497 |
| F | HOH521 |
| site_id | AD8 |
| Number of Residues | 14 |
| Details | binding site for residue MPO F 302 |
| Chain | Residue |
| E | SER238 |
| E | THR242 |
| E | THR243 |
| E | CL303 |
| F | LYS80 |
| F | PRO81 |
| F | ALA106 |
| F | MET126 |
| F | GLY180 |
| F | SER181 |
| F | NAD301 |
| F | HOH420 |
| F | HOH433 |
| F | HOH492 |
| site_id | AD9 |
| Number of Residues | 6 |
| Details | binding site for residue CL F 303 |
| Chain | Residue |
| E | MPO302 |
| F | SER238 |
| F | THR242 |
| F | THR243 |
| F | HOH436 |
| F | HOH536 |
| site_id | AE1 |
| Number of Residues | 20 |
| Details | binding site for residue NAD G 301 |
| Chain | Residue |
| G | GLY17 |
| G | GLY19 |
| G | LYS20 |
| G | MET21 |
| G | HIS45 |
| G | ASN65 |
| G | SER78 |
| G | VAL79 |
| G | LYS80 |
| G | PRO81 |
| G | LEU83 |
| G | VAL87 |
| G | VAL104 |
| G | ALA105 |
| G | ALA106 |
| G | PRO127 |
| G | THR129 |
| G | MPO302 |
| G | HOH408 |
| G | HOH409 |
| site_id | AE2 |
| Number of Residues | 10 |
| Details | binding site for residue MPO G 302 |
| Chain | Residue |
| G | LYS80 |
| G | PRO81 |
| G | ALA106 |
| G | MET126 |
| G | GLY180 |
| G | NAD301 |
| H | SER238 |
| H | THR242 |
| H | THR243 |
| H | CL303 |
| site_id | AE3 |
| Number of Residues | 4 |
| Details | binding site for residue CL G 303 |
| Chain | Residue |
| G | SER238 |
| G | THR243 |
| G | HOH425 |
| H | MPO302 |
| site_id | AE4 |
| Number of Residues | 23 |
| Details | binding site for residue NAD H 301 |
| Chain | Residue |
| H | GLY17 |
| H | GLY19 |
| H | LYS20 |
| H | MET21 |
| H | HIS45 |
| H | ASN65 |
| H | SER78 |
| H | VAL79 |
| H | LYS80 |
| H | PRO81 |
| H | LEU83 |
| H | VAL87 |
| H | VAL104 |
| H | ALA105 |
| H | ALA106 |
| H | PRO127 |
| H | THR129 |
| H | MPO302 |
| H | HOH407 |
| H | HOH424 |
| H | HOH480 |
| H | HOH498 |
| H | HOH499 |
| site_id | AE5 |
| Number of Residues | 14 |
| Details | binding site for residue MPO H 302 |
| Chain | Residue |
| G | SER238 |
| G | THR242 |
| G | THR243 |
| G | CL303 |
| H | LYS80 |
| H | PRO81 |
| H | ALA106 |
| H | MET126 |
| H | THR176 |
| H | GLY180 |
| H | SER181 |
| H | NAD301 |
| H | HOH403 |
| H | HOH473 |
| site_id | AE6 |
| Number of Residues | 5 |
| Details | binding site for residue CL H 303 |
| Chain | Residue |
| G | MPO302 |
| H | VAL236 |
| H | SER238 |
| H | THR243 |
| H | HOH441 |
| site_id | AE7 |
| Number of Residues | 29 |
| Details | binding site for residue NAD I 301 |
| Chain | Residue |
| I | GLY17 |
| I | ALA18 |
| I | GLY19 |
| I | LYS20 |
| I | MET21 |
| I | HIS45 |
| I | ASN65 |
| I | SER78 |
| I | VAL79 |
| I | LYS80 |
| I | PRO81 |
| I | LEU83 |
| I | VAL87 |
| I | VAL104 |
| I | ALA105 |
| I | ALA106 |
| I | PRO127 |
| I | THR129 |
| I | MPO302 |
| I | HOH408 |
| I | HOH413 |
| I | HOH420 |
| I | HOH446 |
| I | HOH485 |
| I | HOH495 |
| I | HOH502 |
| I | HOH513 |
| I | HOH531 |
| I | HOH535 |
| site_id | AE8 |
| Number of Residues | 13 |
| Details | binding site for residue MPO I 302 |
| Chain | Residue |
| I | LYS80 |
| I | PRO81 |
| I | ALA106 |
| I | MET126 |
| I | GLY180 |
| I | SER181 |
| I | NAD301 |
| I | HOH437 |
| I | HOH527 |
| J | SER238 |
| J | THR242 |
| J | THR243 |
| J | CL303 |
| site_id | AE9 |
| Number of Residues | 5 |
| Details | binding site for residue CL I 303 |
| Chain | Residue |
| I | VAL236 |
| I | SER238 |
| I | THR243 |
| I | HOH443 |
| J | MPO302 |
| site_id | AF1 |
| Number of Residues | 24 |
| Details | binding site for residue NAD J 301 |
| Chain | Residue |
| J | GLY17 |
| J | ALA18 |
| J | GLY19 |
| J | LYS20 |
| J | MET21 |
| J | HIS45 |
| J | ASN65 |
| J | SER78 |
| J | VAL79 |
| J | LYS80 |
| J | PRO81 |
| J | LEU83 |
| J | VAL87 |
| J | VAL104 |
| J | ALA105 |
| J | ALA106 |
| J | PRO127 |
| J | THR129 |
| J | MPO302 |
| J | HOH408 |
| J | HOH416 |
| J | HOH420 |
| J | HOH448 |
| J | HOH478 |
| site_id | AF2 |
| Number of Residues | 11 |
| Details | binding site for residue MPO J 302 |
| Chain | Residue |
| I | SER238 |
| I | THR242 |
| I | THR243 |
| I | CL303 |
| J | LYS80 |
| J | PRO81 |
| J | ALA106 |
| J | MET126 |
| J | GLY180 |
| J | SER181 |
| J | NAD301 |
| site_id | AF3 |
| Number of Residues | 4 |
| Details | binding site for residue CL J 303 |
| Chain | Residue |
| I | MPO302 |
| J | SER238 |
| J | THR243 |
| J | HOH414 |
Functional Information from PROSITE/UniProt
| site_id | PS00521 |
| Number of Residues | 23 |
| Details | P5CR Delta 1-pyrroline-5-carboxylate reductase signature. Pgq.LkddVTSpGGtTiaGVheLE |
| Chain | Residue | Details |
| A | PRO229-GLU251 |
