Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

5AQP

Fragment-based screening of HSP70 sheds light on the functional role of ATP-binding site residues

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005524	molecular_function	ATP binding
A	0016887	molecular_function	ATP hydrolysis activity
B	0051087	molecular_function	protein-folding chaperone binding
C	0005524	molecular_function	ATP binding
C	0016887	molecular_function	ATP hydrolysis activity
D	0051087	molecular_function	protein-folding chaperone binding
E	0005524	molecular_function	ATP binding
E	0016887	molecular_function	ATP hydrolysis activity
F	0051087	molecular_function	protein-folding chaperone binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE 1LQ C 1382

Chain	Residue
C	ARG272
C	SER275
C	GLY339
C	ARG342
C	ILE343
C	HOH2155

site_id	AC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE 1LQ A 1382

Chain	Residue
A	ARG342
A	HOH2139
A	ARG272
A	SER275
A	GLY339

site_id	AC3
Number of Residues	5
Details	BINDING SITE FOR RESIDUE 1LQ E 1382

Chain	Residue
E	ARG272
E	SER275
E	GLY339
E	ARG342
E	HOH2075

site_id	AC4
Number of Residues	7
Details	BINDING SITE FOR RESIDUE GOL C 1383

Chain	Residue
C	ASN256
C	ARG258
C	ARG262
C	LEU287
D	LEU218
D	GLN245
D	GOL1262

site_id	AC5
Number of Residues	5
Details	BINDING SITE FOR RESIDUE GOL D 1260

Chain	Residue
D	SER150
D	GLU155
D	ASN229
D	LYS231
D	ASP232

site_id	AC6
Number of Residues	8
Details	BINDING SITE FOR RESIDUE GOL F 1260

Chain	Residue
F	LEU148
F	SER150
F	ASN151
F	GLU155
F	ASN229
F	PHE230
F	LYS231
F	ASP232

site_id	AC7
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GOL A 1383

Chain	Residue
A	ASN256
A	ARG258
A	ARG262
A	SER286
A	LEU287
B	GLN245

site_id	AC8
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GOL B 1261

Chain	Residue
B	ASN151
B	GLU155
B	ASN229
B	PHE230
B	LYS231
B	ASP232

site_id	AC9
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GOL D 1261

Chain	Residue
D	LYS242
D	ALA246
D	ALA246
D	GLU250
D	HOH2032
D	HOH2033

site_id	BC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GOL D 1262

Chain	Residue
C	ARG258
C	GOL1383
C	HOH2138
C	HOH2208
D	GLN245
D	ALA249

site_id	BC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE GOL C 1384

Chain	Residue
C	ARG76
C	TYR149
C	GLN154
C	HOH2082

site_id	BC3
Number of Residues	5
Details	BINDING SITE FOR RESIDUE GOL E 1383

Chain	Residue
E	ASN235
E	ASN239
E	ILE242
E	VAL260
E	ARG264

site_id	BC4
Number of Residues	7
Details	BINDING SITE FOR RESIDUE GOL E 1384

Chain	Residue
E	ASP10
E	GLY12
E	LYS71
E	ASP199
E	GLY201
E	GLY338
E	HOH2004

site_id	BC5
Number of Residues	4
Details	BINDING SITE FOR RESIDUE GOL C 1385

Chain	Residue
C	TYR15
C	THR37
C	ASP366
C	GOL1386

site_id	BC6
Number of Residues	7
Details	BINDING SITE FOR RESIDUE GOL C 1386

Chain	Residue
C	THR14
C	TYR15
C	GLY202
C	ASP366
C	GOL1385
C	TRS1389
C	HOH2009

site_id	BC7
Number of Residues	4
Details	BINDING SITE FOR RESIDUE GOL D 1263

Chain	Residue
C	ALA60
C	HOH2144
D	LYS208
D	GLU255

site_id	BC8
Number of Residues	5
Details	BINDING SITE FOR RESIDUE GOL E 1385

Chain	Residue
E	ASN256
E	ARG262
E	LEU287
F	LEU218
F	GLN245

site_id	BC9
Number of Residues	5
Details	BINDING SITE FOR RESIDUE DMS A 1384

Chain	Residue
A	TYR149
A	HOH2041
A	LYS71
A	ARG72
A	ARG76

site_id	CC1
Number of Residues	5
Details	BINDING SITE FOR RESIDUE DMS C 1387

Chain	Residue
C	LYS71
C	ARG72
C	ARG76
C	TYR149
C	HOH2036

site_id	CC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE DMS C 1388

Chain	Residue
C	ILE28
C	ILE29
C	ALA30
C	ARG36
C	TYR134

site_id	CC3
Number of Residues	5
Details	BINDING SITE FOR RESIDUE DMS A 1385

Chain	Residue
A	ILE28
A	ILE29
A	ALA30
A	ARG36
A	TYR134

site_id	CC4
Number of Residues	5
Details	BINDING SITE FOR RESIDUE DMS E 1386

Chain	Residue
E	ILE28
E	ILE29
E	ALA30
E	ARG36
E	TYR134

site_id	CC5
Number of Residues	7
Details	BINDING SITE FOR RESIDUE TRS A 1386

Chain	Residue
A	ASP10
A	ASP199
A	GLY201
A	GLY338
A	VAL369
A	HOH2014
A	HOH2177

site_id	CC6
Number of Residues	11
Details	BINDING SITE FOR RESIDUE TRS C 1389

Chain	Residue
C	ASP10
C	GLY12
C	LYS71
C	GLU175
C	ASP199
C	GLY201
C	GLY338
C	VAL369
C	GOL1386
C	HOH2014
C	HOH2206

Functional Information from PROSITE/UniProt

site_id	PS00297
Number of Residues	8
Details	HSP70_1 Heat shock hsp70 proteins family signature 1. IDLGTTyS

Chain	Residue	Details
A	ILE9-SER16

site_id	PS00329
Number of Residues	14
Details	HSP70_2 Heat shock hsp70 proteins family signature 2. IFDLGGGTfdvSIL

Chain	Residue	Details
A	ILE197-LEU210

site_id	PS01036
Number of Residues	15
Details	HSP70_3 Heat shock hsp70 proteins family signature 3. IvLvGGsTRIPkIqK

Chain	Residue	Details
A	ILE334-LYS348

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	573
Details	Region: {"description":"Interaction with BAG1"}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	48
Details	Binding site: {}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	21
Details	Binding site: {"evidences":[{"source":"UniProtKB","id":"P19120","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI4
Number of Residues	3
Details	Modified residue: {"description":"N-acetylserine","evidences":[{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"submission","publicationDate":"MAR-2009","submissionDatabase":"UniProtKB","authors":["Bienvenut W.V.","Waridel P.","Quadroni M."]}},{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"submission","publicationDate":"JAN-2010","submissionDatabase":"UniProtKB","authors":["Bienvenut W.V.","Lilla S.","von Kriegsheim A.","Lempens A.","Kolch W.","Bilsland A.E.","Keith W.N."]}},{"source":"PubMed","id":"19413330","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI5
Number of Residues	6
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"UniProtKB","id":"P63017","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI6
Number of Residues	3
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"17525332","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI7
Number of Residues	3
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"PubMed","id":"19608861","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI8
Number of Residues	3
Details	Modified residue: {"description":"N6-succinyllysine; alternate","evidences":[{"source":"UniProtKB","id":"P63017","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI9
Number of Residues	3
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI10
Number of Residues	6
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"20068231","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI11
Number of Residues	240
Details	Domain: {"description":"BAG","evidences":[{"source":"PROSITE-ProRule","id":"PRU00369","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)