5AQK

Fragment-based screening of HSP70 sheds light on the functional role of ATP-binding site residues

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005524	molecular_function	ATP binding
A	0016887	molecular_function	ATP hydrolysis activity
B	0051087	molecular_function	protein-folding chaperone binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	16
Details	BINDING SITE FOR RESIDUE GDP A 1382

Chain	Residue
A	THR14
A	SER340
A	ARG342
A	ILE343
A	TRS1386
A	HOH2046
A	HOH2067
A	HOH2068
A	GLY201
A	GLY202
A	GLY230
A	GLU268
A	LYS271
A	SER275
A	GLY338
A	GLY339

---

site_id	AC2
Number of Residues	10
Details	BINDING SITE FOR RESIDUE GDP B 1260

Chain	Residue
A	ASN239
A	ILE242
A	SER254
A	HOH2053
B	GLU156
B	LEU159
B	LYS160
B	LYS163
B	HIS164
B	HOH2007

---

site_id	AC3
Number of Residues	7
Details	BINDING SITE FOR RESIDUE GOL A 1383

Chain	Residue
A	ASN256
A	ARG258
A	ARG262
A	LEU287
A	GOL1384
B	LEU218
B	GLN245

---

site_id	AC4
Number of Residues	4
Details	BINDING SITE FOR RESIDUE GOL A 1384

Chain	Residue
A	ASN256
A	ARG258
A	GOL1383
B	GLN245

---

site_id	AC5
Number of Residues	4
Details	BINDING SITE FOR RESIDUE GOL A 1385

Chain	Residue
A	MET87
A	TRP90
A	PHE92
A	VAL94

---

site_id	AC6
Number of Residues	9
Details	BINDING SITE FOR RESIDUE TRS A 1386

Chain	Residue
A	ASP10
A	GLY12
A	LYS71
A	GLU175
A	ASP199
A	VAL369
A	GDP1382
A	HOH2009
A	HOH2094

---

site_id	AC7
Number of Residues	5
Details	BINDING SITE FOR RESIDUE DMS A 1387

Chain	Residue
A	GLU27
A	ILE28
A	ILE29
A	ALA30
A	TYR134

---

Functional Information from PROSITE/UniProt

site_id	PS00297
Number of Residues	8
Details	HSP70_1 Heat shock hsp70 proteins family signature 1. IDLGTTyS

Chain	Residue	Details
A	ILE9-SER16

---

site_id	PS00329
Number of Residues	14
Details	HSP70_2 Heat shock hsp70 proteins family signature 2. IFDLGGGTfdvSIL

Chain	Residue	Details
A	ILE197-LEU210

---

site_id	PS01036
Number of Residues	15
Details	HSP70_3 Heat shock hsp70 proteins family signature 3. IvLvGGsTRIPkIqK

Chain	Residue	Details
A	ILE334-LYS348

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	191
Details	Region: {"description":"Interaction with BAG1"}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	16
Details	Binding site: {}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	7
Details	Binding site: {"evidences":[{"source":"UniProtKB","id":"P19120","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"UniProtKB","id":"P63017","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	1
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"17525332","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	1
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"PubMed","id":"19608861","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	1
Details	Modified residue: {"description":"N6-succinyllysine; alternate","evidences":[{"source":"UniProtKB","id":"P63017","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	1
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	2
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"20068231","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	80
Details	Domain: {"description":"BAG","evidences":[{"source":"PROSITE-ProRule","id":"PRU00369","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---