5A7H
Comparison of the structure and activity of glycosylated and aglycosylated Human Carboxylesterase 1
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004771 | molecular_function | sterol ester esterase activity |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005783 | cellular_component | endoplasmic reticulum |
| A | 0005788 | cellular_component | endoplasmic reticulum lumen |
| A | 0005811 | cellular_component | lipid droplet |
| A | 0005829 | cellular_component | cytosol |
| A | 0006629 | biological_process | lipid metabolic process |
| A | 0006695 | biological_process | cholesterol biosynthetic process |
| A | 0008203 | biological_process | cholesterol metabolic process |
| A | 0009636 | biological_process | response to toxic substance |
| A | 0010875 | biological_process | positive regulation of cholesterol efflux |
| A | 0010887 | biological_process | negative regulation of cholesterol storage |
| A | 0016787 | molecular_function | hydrolase activity |
| A | 0019626 | biological_process | short-chain fatty acid catabolic process |
| A | 0030855 | biological_process | epithelial cell differentiation |
| A | 0042572 | biological_process | retinol metabolic process |
| A | 0042632 | biological_process | cholesterol homeostasis |
| A | 0043691 | biological_process | reverse cholesterol transport |
| A | 0047374 | molecular_function | methylumbelliferyl-acetate deacetylase activity |
| A | 0050253 | molecular_function | retinyl-palmitate esterase activity |
| A | 0051791 | biological_process | medium-chain fatty acid metabolic process |
| A | 0052689 | molecular_function | carboxylic ester hydrolase activity |
| A | 0070857 | biological_process | regulation of bile acid biosynthetic process |
| A | 0071397 | biological_process | cellular response to cholesterol |
| A | 0071404 | biological_process | cellular response to low-density lipoprotein particle stimulus |
| A | 0090122 | biological_process | cholesterol ester hydrolysis involved in cholesterol transport |
| A | 0090205 | biological_process | positive regulation of cholesterol metabolic process |
| A | 0106435 | molecular_function | carboxylesterase activity |
| A | 0120188 | biological_process | regulation of bile acid secretion |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE IOD A 1554 |
| Chain | Residue |
| A | SER98 |
| A | ILE108 |
| A | LEU110 |
| A | SER150 |
| A | THR151 |
| A | HOH2047 |
| site_id | AC2 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE IOD A 1555 |
| Chain | Residue |
| A | GLN95 |
| A | LEU110 |
| site_id | AC3 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE IOD A 1557 |
| Chain | Residue |
| A | PRO46 |
| A | ALA48 |
| A | ALA125 |
| A | HOH2061 |
| A | GLN45 |
| site_id | AC4 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE IOD A 1558 |
| Chain | Residue |
| A | SER75 |
| A | IOD1559 |
| site_id | AC5 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE IOD A 1559 |
| Chain | Residue |
| A | SER75 |
| A | ASP182 |
| A | GLU183 |
| A | IOD1558 |
| site_id | AC6 |
| Number of Residues | 1 |
| Details | BINDING SITE FOR RESIDUE IOD A 1560 |
| Chain | Residue |
| A | MET326 |
Functional Information from PROSITE/UniProt
| site_id | PS00122 |
| Number of Residues | 16 |
| Details | CARBOXYLESTERASE_B_1 Carboxylesterases type-B serine active site. FGGnpgsVtIfGeSAG |
| Chain | Residue | Details |
| A | PHE208-GLY223 |
| site_id | PS00941 |
| Number of Residues | 11 |
| Details | CARBOXYLESTERASE_B_2 Carboxylesterases type-B signature 2. EDCLYLNIYtP |
| Chain | Residue | Details |
| A | GLU114-PRO124 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 1 |
| Details | Active site: {"description":"Acyl-ester intermediate","evidences":[{"source":"PROSITE-ProRule","id":"PRU10039","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"12022871","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 2 |
| Details | Active site: {"description":"Charge relay system","evidences":[{"source":"PubMed","id":"12022871","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 1 |
| Details | Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"24275569","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 1 |
| Details | Glycosylation: {"description":"N-linked (GlcNAc...) asparagine","evidences":[{"source":"PubMed","id":"12022871","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
