5A10
The crystal structure of Ta-TFP, a thiocyanate-forming protein involved in glucosinolate breakdown (space group C2)
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0005634 | cellular_component | nucleus |
| A | 0005829 | cellular_component | cytosol |
| A | 0016829 | molecular_function | lyase activity |
| A | 0019760 | biological_process | glucosinolate metabolic process |
| A | 0030234 | molecular_function | enzyme regulator activity |
| A | 0042802 | molecular_function | identical protein binding |
| A | 0042803 | molecular_function | protein homodimerization activity |
| A | 0046872 | molecular_function | metal ion binding |
| A | 0080028 | biological_process | nitrile biosynthetic process |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE ACT A 1349 |
| Chain | Residue |
| A | ARG157 |
| A | GLY185 |
| A | PHE202 |
| A | HOH2318 |
| A | HOH2320 |
| site_id | AC2 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE NA A 1350 |
| Chain | Residue |
| A | ASP33 |
| A | HOH2063 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 1 |
| Details | ACT_SITE: Proton donor => ECO:0000269|PubMed:26260516, ECO:0000305|PubMed:23999604, ECO:0000305|PubMed:28479247, ECO:0000305|PubMed:30900313 |
| Chain | Residue | Details |
| A | ARG94 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 1 |
| Details | ACT_SITE: Proton donor => ECO:0000269|PubMed:26260516, ECO:0000305|PubMed:28479247, ECO:0000305|PubMed:30900313 |
| Chain | Residue | Details |
| A | ARG157 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 1 |
| Details | ACT_SITE: Proton acceptor => ECO:0000305|PubMed:30900313 |
| Chain | Residue | Details |
| A | GLU220 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000305|PubMed:23999604 |
| Chain | Residue | Details |
| A | GLU46 | |
| A | VAL310 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 4 |
| Details | BINDING: BINDING => ECO:0000305|PubMed:23999604, ECO:0000305|PubMed:26260516, ECO:0000305|PubMed:28479247, ECO:0000305|PubMed:30900313 |
| Chain | Residue | Details |
| A | ARG94 | |
| A | GLU266 | |
| A | ASP270 | |
| A | HIS274 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 1 |
| Details | BINDING: BINDING => ECO:0000305|PubMed:28479247 |
| Chain | Residue | Details |
| A | THR129 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 1 |
| Details | BINDING: BINDING => ECO:0000305|PubMed:23999604, ECO:0000305|PubMed:26260516, ECO:0000305|PubMed:30900313 |
| Chain | Residue | Details |
| A | PHE130 |
| site_id | SWS_FT_FI8 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000305|PubMed:26260516, ECO:0000305|PubMed:28479247, ECO:0000305|PubMed:30900313 |
| Chain | Residue | Details |
| A | ARG157 | |
| A | TRP309 |
| site_id | SWS_FT_FI9 |
| Number of Residues | 1 |
| Details | BINDING: BINDING => ECO:0000305|PubMed:23999604, ECO:0000305|PubMed:28479247 |
| Chain | Residue | Details |
| A | ARG269 |
| site_id | SWS_FT_FI10 |
| Number of Residues | 1 |
| Details | SITE: Essential for catalytic activity => ECO:0000269|PubMed:30900313 |
| Chain | Residue | Details |
| A | TYR45 |
| site_id | SWS_FT_FI11 |
| Number of Residues | 1 |
| Details | SITE: Critical for thiocyanate and epithionitrile formation with allylglucosinolate in the presence of myrosinase => ECO:0000269|PubMed:30900313 |
| Chain | Residue | Details |
| A | THR154 |
