521P

THREE-DIMENSIONAL STRUCTURES OF H-RAS P21 MUTANTS: MOLECULAR BASIS FOR THEIR INABILITY TO FUNCTION AS SIGNAL SWITCH MOLECULES

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003924	molecular_function	GTPase activity
A	0005525	molecular_function	GTP binding
A	0007165	biological_process	signal transduction
A	0016020	cellular_component	membrane

Functional Information from PDB Data

site_id	AC1
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG A 168

Chain	Residue
A	SER17
A	THR35
A	GTP167

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site_id	AC2
Number of Residues	21
Details	BINDING SITE FOR RESIDUE GTP A 167

Chain	Residue
A	SER17
A	ALA18
A	PHE28
A	VAL29
A	ASP30
A	TYR32
A	PRO34
A	THR35
A	ASN116
A	LYS117
A	ASP119
A	SER145
A	ALA146
A	LYS147
A	MG168
A	ALA11
A	VAL12
A	GLY13
A	VAL14
A	GLY15
A	LYS16

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	5
Details	BINDING: BINDING => ECO:0000269|PubMed:16698776

Chain	Residue	Details
A	GLY13
A	VAL29
A	THR59
A	ASN116
A	SER145

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site_id	SWS_FT_FI2
Number of Residues	1
Details	MOD_RES: N-acetylmethionine; in GTPase HRas; alternate => ECO:0000269|Ref.12

Chain	Residue	Details
A	MET1

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site_id	SWS_FT_FI3
Number of Residues	1
Details	MOD_RES: N-acetylthreonine; in GTPase HRas, N-terminally processed => ECO:0000269|Ref.12

Chain	Residue	Details
A	THR2

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site_id	SWS_FT_FI4
Number of Residues	1
Details	MOD_RES: S-nitrosocysteine => ECO:0000269|PubMed:9020151

Chain	Residue	Details
A	CYS118

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site_id	SWS_FT_FI5
Number of Residues	1
Details	CARBOHYD: (Microbial infection) O-linked (Glc) threonine; by P.sordellii toxin TcsL => ECO:0000269|PubMed:19744486, ECO:0000269|PubMed:8626575, ECO:0000269|PubMed:8626586, ECO:0000269|PubMed:9632667

Chain	Residue	Details
A	THR35

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