4ZP1
Crystal structure of Zymomonas mobilis pyruvate decarboxylase variant Glu473Ala
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000287 | molecular_function | magnesium ion binding |
| A | 0000949 | biological_process | aromatic amino acid family catabolic process to alcohol via Ehrlich pathway |
| A | 0003824 | molecular_function | catalytic activity |
| A | 0004737 | molecular_function | pyruvate decarboxylase activity |
| A | 0005829 | cellular_component | cytosol |
| A | 0016831 | molecular_function | carboxy-lyase activity |
| A | 0019752 | biological_process | carboxylic acid metabolic process |
| A | 0030976 | molecular_function | thiamine pyrophosphate binding |
| A | 0046872 | molecular_function | metal ion binding |
| B | 0000287 | molecular_function | magnesium ion binding |
| B | 0000949 | biological_process | aromatic amino acid family catabolic process to alcohol via Ehrlich pathway |
| B | 0003824 | molecular_function | catalytic activity |
| B | 0004737 | molecular_function | pyruvate decarboxylase activity |
| B | 0005829 | cellular_component | cytosol |
| B | 0016831 | molecular_function | carboxy-lyase activity |
| B | 0019752 | biological_process | carboxylic acid metabolic process |
| B | 0030976 | molecular_function | thiamine pyrophosphate binding |
| B | 0046872 | molecular_function | metal ion binding |
| C | 0000287 | molecular_function | magnesium ion binding |
| C | 0000949 | biological_process | aromatic amino acid family catabolic process to alcohol via Ehrlich pathway |
| C | 0003824 | molecular_function | catalytic activity |
| C | 0004737 | molecular_function | pyruvate decarboxylase activity |
| C | 0005829 | cellular_component | cytosol |
| C | 0016831 | molecular_function | carboxy-lyase activity |
| C | 0019752 | biological_process | carboxylic acid metabolic process |
| C | 0030976 | molecular_function | thiamine pyrophosphate binding |
| C | 0046872 | molecular_function | metal ion binding |
| D | 0000287 | molecular_function | magnesium ion binding |
| D | 0000949 | biological_process | aromatic amino acid family catabolic process to alcohol via Ehrlich pathway |
| D | 0003824 | molecular_function | catalytic activity |
| D | 0004737 | molecular_function | pyruvate decarboxylase activity |
| D | 0005829 | cellular_component | cytosol |
| D | 0016831 | molecular_function | carboxy-lyase activity |
| D | 0019752 | biological_process | carboxylic acid metabolic process |
| D | 0030976 | molecular_function | thiamine pyrophosphate binding |
| D | 0046872 | molecular_function | metal ion binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 5 |
| Details | binding site for residue MG A 601 |
| Chain | Residue |
| A | ASP440 |
| A | ASN467 |
| A | GLY469 |
| A | TPP603 |
| A | HOH795 |
| site_id | AC2 |
| Number of Residues | 4 |
| Details | binding site for residue NI A 602 |
| Chain | Residue |
| A | HIS106 |
| A | HIS122 |
| C | HIS106 |
| C | HIS122 |
| site_id | AC3 |
| Number of Residues | 22 |
| Details | binding site for residue TPP A 603 |
| Chain | Residue |
| A | ASP390 |
| A | GLY413 |
| A | HIS414 |
| A | ILE415 |
| A | GLY439 |
| A | ASP440 |
| A | GLY441 |
| A | SER442 |
| A | ASN467 |
| A | GLY469 |
| A | TYR470 |
| A | THR471 |
| A | ILE472 |
| A | MG601 |
| A | HOH727 |
| A | HOH795 |
| A | HOH801 |
| B | ALA25 |
| B | GLY26 |
| B | GLU50 |
| B | VAL75 |
| B | HIS114 |
| site_id | AC4 |
| Number of Residues | 5 |
| Details | binding site for residue MG B 601 |
| Chain | Residue |
| B | ASP440 |
| B | ASN467 |
| B | GLY469 |
| B | TPP604 |
| B | HOH713 |
| site_id | AC5 |
| Number of Residues | 5 |
| Details | binding site for residue NI B 602 |
| Chain | Residue |
| B | HIS106 |
| B | HIS122 |
| B | HOH811 |
| D | HIS106 |
| D | HIS122 |
| site_id | AC6 |
| Number of Residues | 9 |
| Details | binding site for residue GOL B 603 |
| Chain | Residue |
| B | ALA89 |
| B | GLU90 |
| B | ASN91 |
| B | SER218 |
| B | ALA245 |
| B | TYR408 |
| B | MET410 |
| B | GLN411 |
| B | HOH714 |
| site_id | AC7 |
| Number of Residues | 21 |
| Details | binding site for residue TPP B 604 |
| Chain | Residue |
| A | ALA25 |
| A | GLY26 |
| A | GLU50 |
| A | VAL75 |
| A | HIS114 |
| B | GLY389 |
| B | ASP390 |
| B | GLY413 |
| B | HIS414 |
| B | ILE415 |
| B | GLY439 |
| B | ASP440 |
| B | GLY441 |
| B | SER442 |
| B | ASN467 |
| B | GLY469 |
| B | TYR470 |
| B | THR471 |
| B | ILE472 |
| B | MG601 |
| B | HOH713 |
| site_id | AC8 |
| Number of Residues | 5 |
| Details | binding site for residue MG C 601 |
| Chain | Residue |
| C | ASP440 |
| C | ASN467 |
| C | GLY469 |
| C | TPP603 |
| C | HOH751 |
| site_id | AC9 |
| Number of Residues | 3 |
| Details | binding site for residue GOL C 602 |
| Chain | Residue |
| C | TYR425 |
| C | ALA429 |
| C | GLU431 |
| site_id | AD1 |
| Number of Residues | 23 |
| Details | binding site for residue TPP C 603 |
| Chain | Residue |
| D | GLU50 |
| D | VAL75 |
| D | HIS114 |
| C | GLY389 |
| C | ASP390 |
| C | GLY413 |
| C | HIS414 |
| C | ILE415 |
| C | GLY439 |
| C | ASP440 |
| C | GLY441 |
| C | SER442 |
| C | ASN467 |
| C | GLY469 |
| C | TYR470 |
| C | THR471 |
| C | ILE472 |
| C | MG601 |
| C | HOH751 |
| C | HOH776 |
| C | HOH827 |
| D | ALA25 |
| D | GLY26 |
| site_id | AD2 |
| Number of Residues | 4 |
| Details | binding site for residue MG D 601 |
| Chain | Residue |
| D | ASP440 |
| D | ASN467 |
| D | GLY469 |
| D | TPP602 |
| site_id | AD3 |
| Number of Residues | 20 |
| Details | binding site for residue TPP D 602 |
| Chain | Residue |
| C | ALA25 |
| C | GLU50 |
| C | VAL75 |
| C | HIS114 |
| D | GLY389 |
| D | ASP390 |
| D | GLY413 |
| D | HIS414 |
| D | ILE415 |
| D | GLY439 |
| D | ASP440 |
| D | GLY441 |
| D | SER442 |
| D | ASN467 |
| D | GLY469 |
| D | TYR470 |
| D | THR471 |
| D | ILE472 |
| D | MG601 |
| D | HOH794 |
Functional Information from PROSITE/UniProt
| site_id | PS00187 |
| Number of Residues | 20 |
| Details | TPP_ENZYMES Thiamine pyrophosphate enzymes signature. FGyavgaPerrnIlMvGDGS |
| Chain | Residue | Details |
| A | PHE423-SER442 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 12 |
| Details | BINDING: BINDING => ECO:0000250 |
| Chain | Residue | Details |
| A | ASP27 | |
| D | ASP27 | |
| D | HIS114 | |
| D | ALA473 | |
| A | HIS114 | |
| A | ALA473 | |
| B | ASP27 | |
| B | HIS114 | |
| B | ALA473 | |
| C | ASP27 | |
| C | HIS114 | |
| C | ALA473 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 16 |
| Details | BINDING: |
| Chain | Residue | Details |
| A | GLU50 | |
| C | ASP440 | |
| C | ASN467 | |
| C | GLY469 | |
| D | GLU50 | |
| D | ASP440 | |
| D | ASN467 | |
| D | GLY469 | |
| A | ASP440 | |
| A | ASN467 | |
| A | GLY469 | |
| B | GLU50 | |
| B | ASP440 | |
| B | ASN467 | |
| B | GLY469 | |
| C | GLU50 |
