4YNG

Twinned pyruvate kinase from E. coli in the T-state

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000287	molecular_function	magnesium ion binding
A	0003824	molecular_function	catalytic activity
A	0004743	molecular_function	pyruvate kinase activity
A	0005524	molecular_function	ATP binding
A	0006096	biological_process	glycolytic process
A	0016301	molecular_function	kinase activity
A	0030955	molecular_function	potassium ion binding
A	0046872	molecular_function	metal ion binding
B	0000287	molecular_function	magnesium ion binding
B	0003824	molecular_function	catalytic activity
B	0004743	molecular_function	pyruvate kinase activity
B	0005524	molecular_function	ATP binding
B	0006096	biological_process	glycolytic process
B	0016301	molecular_function	kinase activity
B	0030955	molecular_function	potassium ion binding
B	0046872	molecular_function	metal ion binding
C	0000287	molecular_function	magnesium ion binding
C	0003824	molecular_function	catalytic activity
C	0004743	molecular_function	pyruvate kinase activity
C	0005524	molecular_function	ATP binding
C	0006096	biological_process	glycolytic process
C	0016301	molecular_function	kinase activity
C	0030955	molecular_function	potassium ion binding
C	0046872	molecular_function	metal ion binding
D	0000287	molecular_function	magnesium ion binding
D	0003824	molecular_function	catalytic activity
D	0004743	molecular_function	pyruvate kinase activity
D	0005524	molecular_function	ATP binding
D	0006096	biological_process	glycolytic process
D	0016301	molecular_function	kinase activity
D	0030955	molecular_function	potassium ion binding
D	0046872	molecular_function	metal ion binding
E	0000287	molecular_function	magnesium ion binding
E	0003824	molecular_function	catalytic activity
E	0004743	molecular_function	pyruvate kinase activity
E	0005524	molecular_function	ATP binding
E	0006096	biological_process	glycolytic process
E	0016301	molecular_function	kinase activity
E	0030955	molecular_function	potassium ion binding
E	0046872	molecular_function	metal ion binding
F	0000287	molecular_function	magnesium ion binding
F	0003824	molecular_function	catalytic activity
F	0004743	molecular_function	pyruvate kinase activity
F	0005524	molecular_function	ATP binding
F	0006096	biological_process	glycolytic process
F	0016301	molecular_function	kinase activity
F	0030955	molecular_function	potassium ion binding
F	0046872	molecular_function	metal ion binding
G	0000287	molecular_function	magnesium ion binding
G	0003824	molecular_function	catalytic activity
G	0004743	molecular_function	pyruvate kinase activity
G	0005524	molecular_function	ATP binding
G	0006096	biological_process	glycolytic process
G	0016301	molecular_function	kinase activity
G	0030955	molecular_function	potassium ion binding
G	0046872	molecular_function	metal ion binding
H	0000287	molecular_function	magnesium ion binding
H	0003824	molecular_function	catalytic activity
H	0004743	molecular_function	pyruvate kinase activity
H	0005524	molecular_function	ATP binding
H	0006096	biological_process	glycolytic process
H	0016301	molecular_function	kinase activity
H	0030955	molecular_function	potassium ion binding
H	0046872	molecular_function	metal ion binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	8
Details	binding site for residue SO4 A 501

Chain	Residue
A	THR378
A	GLN379
A	GLY380
A	GLY381
A	LYS382
A	SER383
A	SER459
A	GLY460

---

site_id	AC2
Number of Residues	9
Details	binding site for residue SO4 B 501

Chain	Residue
B	GLN379
B	GLY380
B	GLY381
B	LYS382
B	SER383
B	SER459
B	GLY460
B	HOH741
B	THR378

---

site_id	AC3
Number of Residues	9
Details	binding site for residue SO4 B 502

Chain	Residue
B	THR9
B	ARG32
B	ASN34
B	SER312
B	GLY313
B	LYS317
B	HOH678
B	HOH778
B	HOH887

---

site_id	AC4
Number of Residues	8
Details	binding site for residue SO4 C 501

Chain	Residue
C	THR378
C	GLN379
C	GLY380
C	GLY381
C	LYS382
C	SER383
C	GLY460
C	HOH694

---

site_id	AC5
Number of Residues	8
Details	binding site for residue SO4 D 501

Chain	Residue
D	THR378
D	GLN379
D	GLY380
D	GLY381
D	LYS382
D	SER383
D	GLY460
D	HOH668

---

site_id	AC6
Number of Residues	4
Details	binding site for residue SO4 D 502

Chain	Residue
D	LYS68
D	LYS220
D	GLU222
D	HOH946

---

site_id	AC7
Number of Residues	6
Details	binding site for residue SO4 E 501

Chain	Residue
E	THR378
E	GLN379
E	GLY380
E	GLY381
E	SER383
E	GLY460

---

site_id	AC8
Number of Residues	10
Details	binding site for residue SO4 F 501

Chain	Residue
F	THR378
F	GLN379
F	GLY380
F	GLY381
F	LYS382
F	SER383
F	SER459
F	THR462
F	HOH654
F	HOH768

---

site_id	AC9
Number of Residues	3
Details	binding site for residue SO4 F 502

Chain	Residue
F	LYS317
F	HOH772
F	HOH846

---

site_id	AD1
Number of Residues	11
Details	binding site for residue SO4 G 501

Chain	Residue
G	THR378
G	GLN379
G	GLY380
G	GLY381
G	LYS382
G	SER383
G	SER459
G	GLY460
G	HOH650
G	HOH712
G	HOH764

---

site_id	AD2
Number of Residues	9
Details	binding site for residue SO4 H 501

Chain	Residue
H	THR378
H	GLN379
H	GLY380
H	GLY381
H	LYS382
H	SER383
H	SER459
H	GLY460
H	HOH665

---

site_id	AD3
Number of Residues	5
Details	binding site for residue SO4 H 502

Chain	Residue
H	LYS68
H	GLU71
H	GLU222
H	HOH733
H	HOH813

---

Functional Information from PROSITE/UniProt

site_id	PS00110
Number of Residues	13
Details	PYRUVATE_KINASE Pyruvate kinase active site signature. IhIISKIENqEGL

Chain	Residue	Details
A	ILE215-LEU227

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	72
Details	BINDING: BINDING => ECO:0000250

Chain	Residue	Details
A	ARG32
B	ARG32
B	ASN34
B	SER36
B	ASP66
B	THR67
B	GLU222
B	GLY245
B	ASP246
B	THR278
C	ARG32
A	ASN34
C	ASN34
C	SER36
C	ASP66
C	THR67
C	GLU222
C	GLY245
C	ASP246
C	THR278
D	ARG32
D	ASN34
A	SER36
D	SER36
D	ASP66
D	THR67
D	GLU222
D	GLY245
D	ASP246
D	THR278
E	ARG32
E	ASN34
E	SER36
A	ASP66
E	ASP66
E	THR67
E	GLU222
E	GLY245
E	ASP246
E	THR278
F	ARG32
F	ASN34
F	SER36
F	ASP66
A	THR67
F	THR67
F	GLU222
F	GLY245
F	ASP246
F	THR278
G	ARG32
G	ASN34
G	SER36
G	ASP66
G	THR67
A	GLU222
G	GLU222
G	GLY245
G	ASP246
G	THR278
H	ARG32
H	ASN34
H	SER36
H	ASP66
H	THR67
H	GLU222
A	GLY245
H	GLY245
H	ASP246
H	THR278
A	ASP246
A	THR278

---

site_id	SWS_FT_FI2
Number of Residues	16
Details	BINDING: BINDING => ECO:0000250|UniProtKB:P14618

Chain	Residue	Details
A	ARG73
E	LYS156
F	ARG73
F	LYS156
G	ARG73
G	LYS156
H	ARG73
H	LYS156
A	LYS156
B	ARG73
B	LYS156
C	ARG73
C	LYS156
D	ARG73
D	LYS156
E	ARG73

---

site_id	SWS_FT_FI3
Number of Residues	8
Details	SITE: Transition state stabilizer => ECO:0000250

Chain	Residue	Details
A	LYS220
B	LYS220
C	LYS220
D	LYS220
E	LYS220
F	LYS220
G	LYS220
H	LYS220

---

site_id	SWS_FT_FI4
Number of Residues	16
Details	MOD_RES: N6-acetyllysine => ECO:0000250

Chain	Residue	Details
A	LYS76
E	LYS319
F	LYS76
F	LYS319
G	LYS76
G	LYS319
H	LYS76
H	LYS319
A	LYS319
B	LYS76
B	LYS319
C	LYS76
C	LYS319
D	LYS76
D	LYS319
E	LYS76

---