4YAW
Reduced CYPOR mutant - G141del
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003420 | biological_process | regulation of growth plate cartilage chondrocyte proliferation |
| A | 0003958 | molecular_function | NADPH-hemoprotein reductase activity |
| A | 0004128 | molecular_function | cytochrome-b5 reductase activity, acting on NAD(P)H |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005783 | cellular_component | endoplasmic reticulum |
| A | 0005789 | cellular_component | endoplasmic reticulum membrane |
| A | 0005829 | cellular_component | cytosol |
| A | 0006809 | biological_process | nitric oxide biosynthetic process |
| A | 0007584 | biological_process | response to nutrient |
| A | 0008941 | molecular_function | nitric oxide dioxygenase NAD(P)H activity |
| A | 0009055 | molecular_function | electron transfer activity |
| A | 0009410 | biological_process | response to xenobiotic stimulus |
| A | 0009437 | biological_process | carnitine metabolic process |
| A | 0009725 | biological_process | response to hormone |
| A | 0009812 | biological_process | flavonoid metabolic process |
| A | 0010181 | molecular_function | FMN binding |
| A | 0016020 | cellular_component | membrane |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0016787 | molecular_function | hydrolase activity |
| A | 0019395 | biological_process | fatty acid oxidation |
| A | 0019899 | molecular_function | enzyme binding |
| A | 0022900 | biological_process | electron transport chain |
| A | 0032332 | biological_process | positive regulation of chondrocyte differentiation |
| A | 0043066 | biological_process | negative regulation of apoptotic process |
| A | 0043231 | cellular_component | intracellular membrane-bounded organelle |
| A | 0043602 | biological_process | nitrate catabolic process |
| A | 0045542 | biological_process | positive regulation of cholesterol biosynthetic process |
| A | 0045880 | biological_process | positive regulation of smoothened signaling pathway |
| A | 0046210 | biological_process | nitric oxide catabolic process |
| A | 0047726 | molecular_function | iron-cytochrome-c reductase activity |
| A | 0050660 | molecular_function | flavin adenine dinucleotide binding |
| A | 0050661 | molecular_function | NADP binding |
| A | 0070988 | biological_process | demethylation |
| A | 0071371 | biological_process | cellular response to gonadotropin stimulus |
| A | 0071372 | biological_process | cellular response to follicle-stimulating hormone stimulus |
| A | 0071375 | biological_process | cellular response to peptide hormone stimulus |
| A | 0071548 | biological_process | response to dexamethasone |
| A | 0090031 | biological_process | positive regulation of steroid hormone biosynthetic process |
| A | 0090181 | biological_process | regulation of cholesterol metabolic process |
| A | 0090346 | biological_process | cellular organofluorine metabolic process |
| B | 0003420 | biological_process | regulation of growth plate cartilage chondrocyte proliferation |
| B | 0003958 | molecular_function | NADPH-hemoprotein reductase activity |
| B | 0004128 | molecular_function | cytochrome-b5 reductase activity, acting on NAD(P)H |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005783 | cellular_component | endoplasmic reticulum |
| B | 0005789 | cellular_component | endoplasmic reticulum membrane |
| B | 0005829 | cellular_component | cytosol |
| B | 0006809 | biological_process | nitric oxide biosynthetic process |
| B | 0007584 | biological_process | response to nutrient |
| B | 0008941 | molecular_function | nitric oxide dioxygenase NAD(P)H activity |
| B | 0009055 | molecular_function | electron transfer activity |
| B | 0009410 | biological_process | response to xenobiotic stimulus |
| B | 0009437 | biological_process | carnitine metabolic process |
| B | 0009725 | biological_process | response to hormone |
| B | 0009812 | biological_process | flavonoid metabolic process |
| B | 0010181 | molecular_function | FMN binding |
| B | 0016020 | cellular_component | membrane |
| B | 0016491 | molecular_function | oxidoreductase activity |
| B | 0016787 | molecular_function | hydrolase activity |
| B | 0019395 | biological_process | fatty acid oxidation |
| B | 0019899 | molecular_function | enzyme binding |
| B | 0022900 | biological_process | electron transport chain |
| B | 0032332 | biological_process | positive regulation of chondrocyte differentiation |
| B | 0043066 | biological_process | negative regulation of apoptotic process |
| B | 0043231 | cellular_component | intracellular membrane-bounded organelle |
| B | 0043602 | biological_process | nitrate catabolic process |
| B | 0045542 | biological_process | positive regulation of cholesterol biosynthetic process |
| B | 0045880 | biological_process | positive regulation of smoothened signaling pathway |
| B | 0046210 | biological_process | nitric oxide catabolic process |
| B | 0047726 | molecular_function | iron-cytochrome-c reductase activity |
| B | 0050660 | molecular_function | flavin adenine dinucleotide binding |
| B | 0050661 | molecular_function | NADP binding |
| B | 0070988 | biological_process | demethylation |
| B | 0071371 | biological_process | cellular response to gonadotropin stimulus |
| B | 0071372 | biological_process | cellular response to follicle-stimulating hormone stimulus |
| B | 0071375 | biological_process | cellular response to peptide hormone stimulus |
| B | 0071548 | biological_process | response to dexamethasone |
| B | 0090031 | biological_process | positive regulation of steroid hormone biosynthetic process |
| B | 0090181 | biological_process | regulation of cholesterol metabolic process |
| B | 0090346 | biological_process | cellular organofluorine metabolic process |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 22 |
| Details | binding site for residue FMN A 701 |
| Chain | Residue |
| A | SER86 |
| A | GLU142 |
| A | GLY143 |
| A | LEU173 |
| A | GLY174 |
| A | ASN175 |
| A | TYR178 |
| A | HIS180 |
| A | PHE181 |
| A | ASN182 |
| A | ASP208 |
| A | GLN87 |
| A | LEU212 |
| A | HOH1013 |
| A | HOH1036 |
| A | THR88 |
| A | GLY89 |
| A | THR90 |
| A | ALA91 |
| A | ALA138 |
| A | THR139 |
| A | TYR140 |
| site_id | AC2 |
| Number of Residues | 26 |
| Details | binding site for residue FAD A 702 |
| Chain | Residue |
| A | HIS319 |
| A | ARG424 |
| A | ARG454 |
| A | TYR455 |
| A | TYR456 |
| A | SER457 |
| A | CYS472 |
| A | ALA473 |
| A | VAL474 |
| A | TYR478 |
| A | GLY488 |
| A | VAL489 |
| A | ALA490 |
| A | THR491 |
| A | TRP677 |
| A | HOH840 |
| A | HOH846 |
| A | HOH905 |
| A | HOH907 |
| A | HOH924 |
| A | HOH949 |
| A | HOH989 |
| A | HOH1064 |
| A | HOH1153 |
| A | HOH1201 |
| A | HOH1242 |
| site_id | AC3 |
| Number of Residues | 16 |
| Details | binding site for residue 2AM A 703 |
| Chain | Residue |
| A | GLY534 |
| A | CYS566 |
| A | ARG567 |
| A | SER596 |
| A | ARG597 |
| A | LYS602 |
| A | TYR604 |
| A | GLN606 |
| A | MET636 |
| A | ASP639 |
| A | PO4706 |
| A | HOH961 |
| A | HOH979 |
| A | HOH1082 |
| A | HOH1087 |
| B | HOH825 |
| site_id | AC4 |
| Number of Residues | 8 |
| Details | binding site for residue PO4 A 704 |
| Chain | Residue |
| A | ASN293 |
| A | GLY295 |
| A | THR296 |
| A | GLU297 |
| A | ARG298 |
| A | ARG567 |
| A | GLU571 |
| A | HOH1045 |
| site_id | AC5 |
| Number of Residues | 6 |
| Details | binding site for residue PO4 A 705 |
| Chain | Residue |
| A | HIS465 |
| A | PRO466 |
| A | ASN467 |
| A | SER468 |
| A | HOH1092 |
| A | HOH1246 |
| site_id | AC6 |
| Number of Residues | 4 |
| Details | binding site for residue PO4 A 706 |
| Chain | Residue |
| A | GLY534 |
| A | THR535 |
| A | 2AM703 |
| A | HOH865 |
| site_id | AC7 |
| Number of Residues | 22 |
| Details | binding site for residue FMN B 701 |
| Chain | Residue |
| B | ASN182 |
| B | ASP208 |
| B | LEU212 |
| B | HOH950 |
| B | HOH988 |
| B | SER86 |
| B | GLN87 |
| B | THR88 |
| B | GLY89 |
| B | THR90 |
| B | ALA91 |
| B | ALA138 |
| B | THR139 |
| B | TYR140 |
| B | GLU142 |
| B | GLY143 |
| B | LEU173 |
| B | GLY174 |
| B | ASN175 |
| B | TYR178 |
| B | HIS180 |
| B | PHE181 |
| site_id | AC8 |
| Number of Residues | 26 |
| Details | binding site for residue FAD B 702 |
| Chain | Residue |
| B | HIS319 |
| B | ARG424 |
| B | ARG454 |
| B | TYR455 |
| B | TYR456 |
| B | SER457 |
| B | CYS472 |
| B | ALA473 |
| B | VAL474 |
| B | TYR478 |
| B | GLY488 |
| B | VAL489 |
| B | ALA490 |
| B | THR491 |
| B | TRP677 |
| B | HOH843 |
| B | HOH873 |
| B | HOH883 |
| B | HOH887 |
| B | HOH908 |
| B | HOH926 |
| B | HOH935 |
| B | HOH965 |
| B | HOH1018 |
| B | HOH1035 |
| B | HOH1151 |
| site_id | AC9 |
| Number of Residues | 18 |
| Details | binding site for residue 2AM B 703 |
| Chain | Residue |
| B | GLY534 |
| B | CYS566 |
| B | ARG567 |
| B | SER596 |
| B | ARG597 |
| B | LYS602 |
| B | TYR604 |
| B | GLN606 |
| B | MET636 |
| B | ASP639 |
| B | PO4706 |
| B | HOH845 |
| B | HOH865 |
| B | HOH953 |
| B | HOH994 |
| B | HOH1046 |
| B | HOH1078 |
| B | HOH1108 |
| site_id | AD1 |
| Number of Residues | 8 |
| Details | binding site for residue PO4 B 704 |
| Chain | Residue |
| B | ASN293 |
| B | GLY295 |
| B | THR296 |
| B | GLU297 |
| B | ARG298 |
| B | ARG567 |
| B | GLU571 |
| B | HOH1113 |
| site_id | AD2 |
| Number of Residues | 4 |
| Details | binding site for residue PO4 B 705 |
| Chain | Residue |
| B | HIS465 |
| B | PRO466 |
| B | ASN467 |
| B | SER468 |
| site_id | AD3 |
| Number of Residues | 4 |
| Details | binding site for residue PO4 B 706 |
| Chain | Residue |
| B | GLY534 |
| B | THR535 |
| B | 2AM703 |
| B | HOH866 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 20 |
| Details | BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_03212, ECO:0000269|PubMed:11371558, ECO:0000269|PubMed:19171935, ECO:0000269|PubMed:21345800, ECO:0000269|PubMed:9237990, ECO:0000269|Ref.8 |
| Chain | Residue | Details |
| A | SER86 | |
| A | SER678 | |
| B | SER86 | |
| B | ALA138 | |
| B | GLY174 | |
| B | TYR455 | |
| B | GLU479 | |
| B | VAL489 | |
| B | GLY536 | |
| B | ARG597 | |
| B | VAL603 | |
| A | ALA138 | |
| B | SER678 | |
| A | GLY174 | |
| A | TYR455 | |
| A | GLU479 | |
| A | VAL489 | |
| A | GLY536 | |
| A | ARG597 | |
| A | VAL603 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_03212, ECO:0000269|PubMed:11371558, ECO:0000269|PubMed:21345800, ECO:0000269|PubMed:9237990 |
| Chain | Residue | Details |
| A | ASP209 | |
| B | ASP209 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 4 |
| Details | BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_03212, ECO:0000269|PubMed:11371558, ECO:0000269|PubMed:19171935, ECO:0000269|PubMed:21345800, ECO:0000269|PubMed:9237990 |
| Chain | Residue | Details |
| A | HIS299 | |
| A | VAL640 | |
| B | HIS299 | |
| B | VAL640 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_03212, ECO:0000269|PubMed:11371558, ECO:0000269|PubMed:19171935, ECO:0000269|PubMed:21345800, ECO:0000269|Ref.8 |
| Chain | Residue | Details |
| A | ARG425 | |
| B | ARG425 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_03212, ECO:0000269|PubMed:11371558, ECO:0000269|PubMed:19171935, ECO:0000269|PubMed:9237990, ECO:0000269|Ref.8 |
| Chain | Residue | Details |
| A | ALA473 | |
| B | ALA473 |
Catalytic Information from CSA
| site_id | MCSA1 |
| Number of Residues | 4 |
| Details | M-CSA 117 |
| Chain | Residue | Details |
| A | ILE458 | hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor, proton relay, radical stabiliser |
| A | GLY631 | electrostatic stabiliser, hydrogen bond donor, proton acceptor, proton donor, proton relay, steric role, van der waals interaction |
| A | VAL676 | hydrogen bond acceptor, modifies pKa |
| A | SER678 | steric role |
| site_id | MCSA2 |
| Number of Residues | 4 |
| Details | M-CSA 117 |
| Chain | Residue | Details |
| B | ILE458 | hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor, proton relay, radical stabiliser |
| B | GLY631 | electrostatic stabiliser, hydrogen bond donor, proton acceptor, proton donor, proton relay, steric role, van der waals interaction |
| B | VAL676 | hydrogen bond acceptor, modifies pKa |
| B | SER678 | steric role |
