4XNB
Crystal Structure of E. coli Aminopeptidase N in complex with L-Beta Homophenylalanine
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004177 | molecular_function | aminopeptidase activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005886 | cellular_component | plasma membrane |
| A | 0006508 | biological_process | proteolysis |
| A | 0008233 | molecular_function | peptidase activity |
| A | 0008237 | molecular_function | metallopeptidase activity |
| A | 0008270 | molecular_function | zinc ion binding |
| A | 0016285 | molecular_function | alanyl aminopeptidase activity |
| A | 0016787 | molecular_function | hydrolase activity |
| A | 0042802 | molecular_function | identical protein binding |
| A | 0046872 | molecular_function | metal ion binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 4 |
| Details | binding site for residue ZN A 901 |
| Chain | Residue |
| A | HIS297 |
| A | HIS301 |
| A | GLU320 |
| A | 3FB902 |
| site_id | AC2 |
| Number of Residues | 15 |
| Details | binding site for residue 3FB A 902 |
| Chain | Residue |
| A | GLU264 |
| A | HIS297 |
| A | GLU298 |
| A | HIS301 |
| A | LYS319 |
| A | GLU320 |
| A | TYR376 |
| A | TYR381 |
| A | ZN901 |
| A | GOL930 |
| A | GLN119 |
| A | GLU121 |
| A | MET260 |
| A | ALA262 |
| A | MET263 |
| site_id | AC3 |
| Number of Residues | 5 |
| Details | binding site for residue NA A 903 |
| Chain | Residue |
| A | GLN19 |
| A | ILE20 |
| A | LEU138 |
| A | HOH1738 |
| A | HOH1906 |
| site_id | AC4 |
| Number of Residues | 5 |
| Details | binding site for residue NA A 904 |
| Chain | Residue |
| A | ASN67 |
| A | THR91 |
| A | HOH1872 |
| A | HOH1887 |
| A | HOH1907 |
| site_id | AC5 |
| Number of Residues | 5 |
| Details | binding site for residue NA A 905 |
| Chain | Residue |
| A | HIS771 |
| A | PHE774 |
| A | HOH1172 |
| A | HOH1716 |
| A | HOH1810 |
| site_id | AC6 |
| Number of Residues | 7 |
| Details | binding site for residue NA A 906 |
| Chain | Residue |
| A | ASP452 |
| A | NA910 |
| A | GOL915 |
| A | HOH1058 |
| A | HOH1683 |
| A | HOH1798 |
| A | HOH1817 |
| site_id | AC7 |
| Number of Residues | 5 |
| Details | binding site for residue NA A 907 |
| Chain | Residue |
| A | ASP453 |
| A | TYR454 |
| A | HOH1247 |
| A | HOH1496 |
| A | HOH1729 |
| site_id | AC8 |
| Number of Residues | 7 |
| Details | binding site for residue NA A 908 |
| Chain | Residue |
| A | SER332 |
| A | ASP333 |
| A | GLY335 |
| A | HOH1038 |
| A | HOH1513 |
| A | HOH1773 |
| A | HOH1846 |
| site_id | AC9 |
| Number of Residues | 6 |
| Details | binding site for residue NA A 909 |
| Chain | Residue |
| A | GLU107 |
| A | GLU371 |
| A | HOH1143 |
| A | HOH1144 |
| A | HOH1228 |
| A | HOH1460 |
| site_id | AD1 |
| Number of Residues | 6 |
| Details | binding site for residue NA A 910 |
| Chain | Residue |
| A | ASP452 |
| A | NA906 |
| A | HOH1037 |
| A | HOH1798 |
| A | HOH1873 |
| A | HOH1879 |
| site_id | AD2 |
| Number of Residues | 9 |
| Details | binding site for residue GOL A 911 |
| Chain | Residue |
| A | LYS8 |
| A | GLU123 |
| A | ASP312 |
| A | TRP313 |
| A | PHE314 |
| A | HOH1140 |
| A | HOH1479 |
| A | HOH1503 |
| A | HOH1645 |
| site_id | AD3 |
| Number of Residues | 8 |
| Details | binding site for residue GOL A 912 |
| Chain | Residue |
| A | PRO473 |
| A | ASP474 |
| A | GLN475 |
| A | ALA476 |
| A | HOH1065 |
| A | HOH1090 |
| A | HOH1181 |
| A | HOH1557 |
| site_id | AD4 |
| Number of Residues | 3 |
| Details | binding site for residue GOL A 913 |
| Chain | Residue |
| A | LYS144 |
| A | TRP173 |
| A | HOH1015 |
| site_id | AD5 |
| Number of Residues | 10 |
| Details | binding site for residue GOL A 914 |
| Chain | Residue |
| A | SER63 |
| A | VAL64 |
| A | TRP71 |
| A | TRP74 |
| A | ARG669 |
| A | VAL670 |
| A | GLU671 |
| A | GOL923 |
| A | MLI933 |
| A | HOH1422 |
| site_id | AD6 |
| Number of Residues | 11 |
| Details | binding site for residue GOL A 915 |
| Chain | Residue |
| A | NA906 |
| A | HOH1022 |
| A | HOH1023 |
| A | HOH1025 |
| A | HOH1058 |
| A | HOH1517 |
| A | HOH1683 |
| A | ASP452 |
| A | TYR544 |
| A | LYS545 |
| A | GLN550 |
| site_id | AD7 |
| Number of Residues | 8 |
| Details | binding site for residue GOL A 916 |
| Chain | Residue |
| A | LEU542 |
| A | GLU543 |
| A | TYR544 |
| A | TRP546 |
| A | HOH1035 |
| A | HOH1195 |
| A | HOH1364 |
| A | HOH1524 |
| site_id | AD8 |
| Number of Residues | 6 |
| Details | binding site for residue GOL A 917 |
| Chain | Residue |
| A | THR284 |
| A | ASP285 |
| A | LYS286 |
| A | HOH1013 |
| A | HOH1123 |
| A | HOH1534 |
| site_id | AD9 |
| Number of Residues | 4 |
| Details | binding site for residue GOL A 918 |
| Chain | Residue |
| A | GLU292 |
| A | VAL670 |
| A | HIS672 |
| A | HOH1462 |
| site_id | AE1 |
| Number of Residues | 6 |
| Details | binding site for residue GOL A 919 |
| Chain | Residue |
| A | THR393 |
| A | CYS534 |
| A | GLU535 |
| A | HOH1047 |
| A | HOH1098 |
| A | HOH1577 |
| site_id | AE2 |
| Number of Residues | 7 |
| Details | binding site for residue GOL A 920 |
| Chain | Residue |
| A | ARG346 |
| A | THR347 |
| A | LEU351 |
| A | SER621 |
| A | ASN623 |
| A | GLU624 |
| A | HOH1082 |
| site_id | AE3 |
| Number of Residues | 4 |
| Details | binding site for residue GOL A 921 |
| Chain | Residue |
| A | LEU532 |
| A | TRP546 |
| A | ASP566 |
| A | SER570 |
| site_id | AE4 |
| Number of Residues | 5 |
| Details | binding site for residue GOL A 922 |
| Chain | Residue |
| A | ARG14 |
| A | ALA15 |
| A | PRO16 |
| A | ASP17 |
| A | HOH1227 |
| site_id | AE5 |
| Number of Residues | 6 |
| Details | binding site for residue GOL A 923 |
| Chain | Residue |
| A | THR72 |
| A | TRP74 |
| A | VAL670 |
| A | GOL914 |
| A | HOH1462 |
| A | HOH1794 |
| site_id | AE6 |
| Number of Residues | 6 |
| Details | binding site for residue GOL A 924 |
| Chain | Residue |
| A | GLU409 |
| A | ASP412 |
| A | SER590 |
| A | LEU591 |
| A | HOH1300 |
| A | HOH1629 |
| site_id | AE7 |
| Number of Residues | 5 |
| Details | binding site for residue GOL A 925 |
| Chain | Residue |
| A | TYR376 |
| A | LEU378 |
| A | GLU382 |
| A | GOL930 |
| A | MLI934 |
| site_id | AE8 |
| Number of Residues | 8 |
| Details | binding site for residue GOL A 926 |
| Chain | Residue |
| A | ARG641 |
| A | THR645 |
| A | ARG686 |
| A | PHE690 |
| A | ALA722 |
| A | GOL928 |
| A | HOH1029 |
| A | HOH1272 |
| site_id | AE9 |
| Number of Residues | 9 |
| Details | binding site for residue GOL A 927 |
| Chain | Residue |
| A | VAL622 |
| A | THR755 |
| A | PRO757 |
| A | SER792 |
| A | ASN793 |
| A | GOL928 |
| A | HOH1029 |
| A | HOH1298 |
| A | HOH1523 |
| site_id | AF1 |
| Number of Residues | 10 |
| Details | binding site for residue GOL A 928 |
| Chain | Residue |
| A | ALA638 |
| A | GLU642 |
| A | PHE690 |
| A | ALA722 |
| A | PRO757 |
| A | GOL926 |
| A | GOL927 |
| A | HOH1006 |
| A | HOH1029 |
| A | HOH1034 |
| site_id | AF2 |
| Number of Residues | 8 |
| Details | binding site for residue GOL A 929 |
| Chain | Residue |
| A | ARG681 |
| A | GLN703 |
| A | ALA707 |
| A | ASN708 |
| A | ASN709 |
| A | ASP712 |
| A | HOH1095 |
| A | HOH1545 |
| site_id | AF3 |
| Number of Residues | 8 |
| Details | binding site for residue GOL A 930 |
| Chain | Residue |
| A | ARG293 |
| A | HIS297 |
| A | GLU298 |
| A | 3FB902 |
| A | GOL925 |
| A | HOH1070 |
| A | HOH1121 |
| A | HOH1166 |
| site_id | AF4 |
| Number of Residues | 5 |
| Details | binding site for residue GOL A 931 |
| Chain | Residue |
| A | LYS402 |
| A | GLN405 |
| A | GLU409 |
| A | HOH1041 |
| A | HOH1042 |
| site_id | AF5 |
| Number of Residues | 11 |
| Details | binding site for residue GOL A 932 |
| Chain | Residue |
| A | LEU289 |
| A | ASP290 |
| A | ARG293 |
| A | ASN340 |
| A | HOH1002 |
| A | HOH1017 |
| A | HOH1055 |
| A | HOH1137 |
| A | HOH1277 |
| A | HOH1349 |
| A | HOH1693 |
| site_id | AF6 |
| Number of Residues | 10 |
| Details | binding site for residue MLI A 933 |
| Chain | Residue |
| A | LEU61 |
| A | VAL62 |
| A | VAL64 |
| A | TRP74 |
| A | ARG669 |
| A | GLU671 |
| A | GOL914 |
| A | HOH1014 |
| A | HOH1226 |
| A | HOH1512 |
| site_id | AF7 |
| Number of Residues | 8 |
| Details | binding site for residue MLI A 934 |
| Chain | Residue |
| A | MET260 |
| A | GLY261 |
| A | LYS274 |
| A | TYR275 |
| A | ARG783 |
| A | ARG825 |
| A | GOL925 |
| A | HOH1121 |
| site_id | AF8 |
| Number of Residues | 5 |
| Details | binding site for residue MLI A 935 |
| Chain | Residue |
| A | SER441 |
| A | GLN442 |
| A | GLN475 |
| A | GLU477 |
| A | GLN479 |
| site_id | AF9 |
| Number of Residues | 9 |
| Details | binding site for residue MLI A 936 |
| Chain | Residue |
| A | GLY787 |
| A | GLY791 |
| A | ARG825 |
| A | LEU826 |
| A | PRO829 |
| A | HOH1084 |
| A | HOH1190 |
| A | HOH1338 |
| A | HOH1725 |
Functional Information from PROSITE/UniProt
| site_id | PS00142 |
| Number of Residues | 10 |
| Details | ZINC_PROTEASE Neutral zinc metallopeptidases, zinc-binding region signature. VIGHEYFHNW |
| Chain | Residue | Details |
| A | VAL294-TRP303 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 1 |
| Details | Active site: {"description":"Proton acceptor","evidences":[{"source":"PubMed","id":"16885166","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"18416562","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"19622865","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 8 |
| Details | Binding site: {} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 1 |
| Details | Site: {"description":"Transition state stabilizer","evidences":[{"evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
