4XFP

Crystal Structure of Highly Active Mutant of Bacillus sp. TB-90 Urate Oxidase

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004846	molecular_function	urate oxidase activity
A	0006144	biological_process	purine nucleobase metabolic process
B	0004846	molecular_function	urate oxidase activity
B	0006144	biological_process	purine nucleobase metabolic process
C	0004846	molecular_function	urate oxidase activity
C	0006144	biological_process	purine nucleobase metabolic process
D	0004846	molecular_function	urate oxidase activity
D	0006144	biological_process	purine nucleobase metabolic process

Functional Information from PDB Data

site_id	AC1
Number of Residues	13
Details	binding site for residue AZA A 401

Chain	Residue
A	PHE184
A	HOH696
B	TYR11
B	ALA72
B	THR73
A	LEU195
A	ARG201
A	SER248
A	ILE249
A	GLN250
A	ASN276
A	CL402
A	HOH622

---

site_id	AC2
Number of Residues	5
Details	binding site for residue CL A 402

Chain	Residue
A	ASN276
A	GLY302
A	GLN304
A	AZA401
B	THR73

---

site_id	AC3
Number of Residues	2
Details	binding site for residue SO4 A 403

Chain	Residue
A	ARG298
C	ARG298

---

site_id	AC4
Number of Residues	11
Details	binding site for residue AZA B 401

Chain	Residue
A	ALA72
A	THR73
B	PHE184
B	LEU195
B	ARG201
B	SER248
B	ILE249
B	GLN250
B	ASN276
B	CL402
B	HOH588

---

site_id	AC5
Number of Residues	6
Details	binding site for residue CL B 402

Chain	Residue
A	THR73
A	HOH710
B	ASN276
B	GLY302
B	GLN304
B	AZA401

---

site_id	AC6
Number of Residues	10
Details	binding site for residue AZA C 401

Chain	Residue
C	PHE184
C	ARG201
C	SER248
C	ILE249
C	GLN250
C	ASN276
C	CL402
C	HOH573
D	ALA72
D	THR73

---

site_id	AC7
Number of Residues	5
Details	binding site for residue CL C 402

Chain	Residue
C	ASN276
C	GLY302
C	GLN304
C	AZA401
D	THR73

---

site_id	AC8
Number of Residues	2
Details	binding site for residue SO4 D 401

Chain	Residue
B	ARG298
D	ARG298

---

site_id	AC9
Number of Residues	11
Details	binding site for residue AZA D 402

Chain	Residue
C	ALA72
C	THR73
C	HOH701
D	PHE184
D	ARG201
D	SER248
D	ILE249
D	GLN250
D	ASN276
D	CL403
D	HOH592

---

site_id	AD1
Number of Residues	6
Details	binding site for residue CL D 403

Chain	Residue
C	THR73
D	ASN276
D	GLY302
D	GLN304
D	AZA402
D	HOH698

---

Functional Information from PROSITE/UniProt

site_id	PS00366
Number of Residues	28
Details	URICASE Uricase signature. LqLIKVSgNsFvgFirdeYttLpedsnR

Chain	Residue	Details
A	LEU174-ARG201

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	8
Details	ACT_SITE: Charge relay system; for urate oxidase activity => ECO:0000250|UniProtKB:D0VWQ1

Chain	Residue	Details
A	LYS13
A	THR73
B	LYS13
B	THR73
C	LYS13
C	THR73
D	LYS13
D	THR73

---

site_id	SWS_FT_FI2
Number of Residues	4
Details	ACT_SITE: Charge relay system => ECO:0000250|UniProtKB:Q00511

Chain	Residue	Details
A	LYS24
B	LYS24
C	LYS24
D	LYS24

---

site_id	SWS_FT_FI3
Number of Residues	20
Details	BINDING: BINDING => ECO:0000250|UniProtKB:Q00511

Chain	Residue	Details
A	THR73
B	ASN276
C	THR73
C	ASP74
C	ILE249
C	GLN250
C	ASN276
D	THR73
D	ASP74
D	ILE249
D	GLN250
A	ASP74
D	ASN276
A	ILE249
A	GLN250
A	ASN276
B	THR73
B	ASP74
B	ILE249
B	GLN250

---

site_id	SWS_FT_FI4
Number of Residues	8
Details	BINDING: BINDING => ECO:0000305|Ref.3

Chain	Residue	Details
A	PHE184
A	ARG201
B	PHE184
B	ARG201
C	PHE184
C	ARG201
D	PHE184
D	ARG201

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