4WR3

Y274F alanine racemase from E. coli

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003824	molecular_function	catalytic activity
A	0005829	cellular_component	cytosol
A	0006522	biological_process	alanine metabolic process
A	0008360	biological_process	regulation of cell shape
A	0008784	molecular_function	alanine racemase activity
A	0009252	biological_process	peptidoglycan biosynthetic process
A	0016853	molecular_function	isomerase activity
A	0030170	molecular_function	pyridoxal phosphate binding
A	0030632	biological_process	D-alanine biosynthetic process
A	0042803	molecular_function	protein homodimerization activity
A	0071555	biological_process	cell wall organization
B	0003824	molecular_function	catalytic activity
B	0005829	cellular_component	cytosol
B	0006522	biological_process	alanine metabolic process
B	0008360	biological_process	regulation of cell shape
B	0008784	molecular_function	alanine racemase activity
B	0009252	biological_process	peptidoglycan biosynthetic process
B	0016853	molecular_function	isomerase activity
B	0030170	molecular_function	pyridoxal phosphate binding
B	0030632	biological_process	D-alanine biosynthetic process
B	0042803	molecular_function	protein homodimerization activity
B	0071555	biological_process	cell wall organization
C	0003824	molecular_function	catalytic activity
C	0005829	cellular_component	cytosol
C	0006522	biological_process	alanine metabolic process
C	0008360	biological_process	regulation of cell shape
C	0008784	molecular_function	alanine racemase activity
C	0009252	biological_process	peptidoglycan biosynthetic process
C	0016853	molecular_function	isomerase activity
C	0030170	molecular_function	pyridoxal phosphate binding
C	0030632	biological_process	D-alanine biosynthetic process
C	0042803	molecular_function	protein homodimerization activity
C	0071555	biological_process	cell wall organization
D	0003824	molecular_function	catalytic activity
D	0005829	cellular_component	cytosol
D	0006522	biological_process	alanine metabolic process
D	0008360	biological_process	regulation of cell shape
D	0008784	molecular_function	alanine racemase activity
D	0009252	biological_process	peptidoglycan biosynthetic process
D	0016853	molecular_function	isomerase activity
D	0030170	molecular_function	pyridoxal phosphate binding
D	0030632	biological_process	D-alanine biosynthetic process
D	0042803	molecular_function	protein homodimerization activity
D	0071555	biological_process	cell wall organization

Functional Information from PDB Data

site_id	AC1
Number of Residues	11
Details	binding site for residue PLP A 1001

Chain	Residue
A	TYR38
A	HOH1210
B	GOL1004
A	HIS159
A	ALA193
A	SER194
A	ARG209
A	GLY211
A	ILE212
A	TYR343
A	HOH1161

---

site_id	AC2
Number of Residues	3
Details	binding site for residue SO4 A 1002

Chain	Residue
A	ARG129
A	LEU130
A	HIS159

---

site_id	AC3
Number of Residues	2
Details	binding site for residue SO4 A 1003

Chain	Residue
A	ARG266
B	ARG19

---

site_id	AC4
Number of Residues	5
Details	binding site for residue GOL A 1004

Chain	Residue
A	TYR255
A	ALA302
A	MET303
A	ASP304
B	ARG129

---

site_id	AC5
Number of Residues	11
Details	binding site for residue PLP B 1001

Chain	Residue
B	TYR38
B	HIS159
B	ALA193
B	SER194
B	ARG209
B	GLY211
B	ILE212
B	TYR343
B	HOH1164
B	HOH1222
B	HOH1239

---

site_id	AC6
Number of Residues	4
Details	binding site for residue SO4 B 1002

Chain	Residue
B	ARG129
B	LEU130
B	HIS159
B	HOH1251

---

site_id	AC7
Number of Residues	4
Details	binding site for residue SO4 B 1003

Chain	Residue
A	ARG19
B	ARG266
B	HOH1244
B	HOH1262

---

site_id	AC8
Number of Residues	7
Details	binding site for residue GOL B 1004

Chain	Residue
A	ARG129
A	PLP1001
B	TYR255
B	ALA302
B	MET303
B	ASP304
B	MET305

---

site_id	AC9
Number of Residues	9
Details	binding site for residue PLP C 1001

Chain	Residue
C	TYR38
C	ALA193
C	SER194
C	ARG209
C	GLY211
C	ILE212
C	TYR343
C	HOH1105
D	GOL1002

---

site_id	AD1
Number of Residues	2
Details	binding site for residue SO4 C 1002

Chain	Residue
C	ARG19
D	ARG266

---

site_id	AD2
Number of Residues	7
Details	binding site for residue PLP D 1001

Chain	Residue
D	TYR38
D	ALA193
D	SER194
D	ARG209
D	GLY211
D	ILE212
D	TYR343

---

site_id	AD3
Number of Residues	7
Details	binding site for residue GOL D 1002

Chain	Residue
C	ARG129
C	PLP1001
D	TYR255
D	ALA302
D	MET303
D	ASP304
D	MET305

---

Functional Information from PROSITE/UniProt

site_id	PS00395
Number of Residues	11
Details	ALANINE_RACEMASE Alanine racemase pyridoxal-phosphate attachment site. AVvKANAYGHG

Chain	Residue	Details
A	ALA31-GLY41

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	Active site: {"description":"Proton acceptor; specific for D-alanine","evidences":[{"source":"HAMAP-Rule","id":"MF_01201","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	4
Details	Active site: {"description":"Proton acceptor; specific for L-alanine","evidences":[{"source":"HAMAP-Rule","id":"MF_01201","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	8
Details	Binding site: {"evidences":[{"source":"PubMed","id":"18434499","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	4
Details	Modified residue: {"description":"N6-(pyridoxal phosphate)lysine","evidences":[{"source":"PubMed","id":"18434499","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	4
Details	Modified residue: {"description":"N6-carboxylysine","evidences":[{"source":"PubMed","id":"18434499","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---