4WLE

Crystal structure of citrate bound MDH2

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003723	molecular_function	RNA binding
A	0003824	molecular_function	catalytic activity
A	0005634	cellular_component	nucleus
A	0005737	cellular_component	cytoplasm
A	0005739	cellular_component	mitochondrion
A	0005759	cellular_component	mitochondrial matrix
A	0006094	biological_process	gluconeogenesis
A	0006099	biological_process	tricarboxylic acid cycle
A	0006107	biological_process	oxaloacetate metabolic process
A	0006108	biological_process	malate metabolic process
A	0006734	biological_process	NADH metabolic process
A	0009060	biological_process	aerobic respiration
A	0016020	cellular_component	membrane
A	0016491	molecular_function	oxidoreductase activity
A	0016615	molecular_function	malate dehydrogenase activity
A	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
A	0019752	biological_process	carboxylic acid metabolic process
A	0030060	molecular_function	L-malate dehydrogenase activity
A	0043490	biological_process	malate-aspartate shuttle
A	0043621	molecular_function	obsolete protein self-association
A	0046554	molecular_function	malate dehydrogenase (NADP+) activity
A	0070062	cellular_component	extracellular exosome
B	0003723	molecular_function	RNA binding
B	0003824	molecular_function	catalytic activity
B	0005634	cellular_component	nucleus
B	0005737	cellular_component	cytoplasm
B	0005739	cellular_component	mitochondrion
B	0005759	cellular_component	mitochondrial matrix
B	0006094	biological_process	gluconeogenesis
B	0006099	biological_process	tricarboxylic acid cycle
B	0006107	biological_process	oxaloacetate metabolic process
B	0006108	biological_process	malate metabolic process
B	0006734	biological_process	NADH metabolic process
B	0009060	biological_process	aerobic respiration
B	0016020	cellular_component	membrane
B	0016491	molecular_function	oxidoreductase activity
B	0016615	molecular_function	malate dehydrogenase activity
B	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
B	0019752	biological_process	carboxylic acid metabolic process
B	0030060	molecular_function	L-malate dehydrogenase activity
B	0043490	biological_process	malate-aspartate shuttle
B	0043621	molecular_function	obsolete protein self-association
B	0046554	molecular_function	malate dehydrogenase (NADP+) activity
B	0070062	cellular_component	extracellular exosome
C	0003723	molecular_function	RNA binding
C	0003824	molecular_function	catalytic activity
C	0005634	cellular_component	nucleus
C	0005737	cellular_component	cytoplasm
C	0005739	cellular_component	mitochondrion
C	0005759	cellular_component	mitochondrial matrix
C	0006094	biological_process	gluconeogenesis
C	0006099	biological_process	tricarboxylic acid cycle
C	0006107	biological_process	oxaloacetate metabolic process
C	0006108	biological_process	malate metabolic process
C	0006734	biological_process	NADH metabolic process
C	0009060	biological_process	aerobic respiration
C	0016020	cellular_component	membrane
C	0016491	molecular_function	oxidoreductase activity
C	0016615	molecular_function	malate dehydrogenase activity
C	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
C	0019752	biological_process	carboxylic acid metabolic process
C	0030060	molecular_function	L-malate dehydrogenase activity
C	0043490	biological_process	malate-aspartate shuttle
C	0043621	molecular_function	obsolete protein self-association
C	0046554	molecular_function	malate dehydrogenase (NADP+) activity
C	0070062	cellular_component	extracellular exosome
D	0003723	molecular_function	RNA binding
D	0003824	molecular_function	catalytic activity
D	0005634	cellular_component	nucleus
D	0005737	cellular_component	cytoplasm
D	0005739	cellular_component	mitochondrion
D	0005759	cellular_component	mitochondrial matrix
D	0006094	biological_process	gluconeogenesis
D	0006099	biological_process	tricarboxylic acid cycle
D	0006107	biological_process	oxaloacetate metabolic process
D	0006108	biological_process	malate metabolic process
D	0006734	biological_process	NADH metabolic process
D	0009060	biological_process	aerobic respiration
D	0016020	cellular_component	membrane
D	0016491	molecular_function	oxidoreductase activity
D	0016615	molecular_function	malate dehydrogenase activity
D	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
D	0019752	biological_process	carboxylic acid metabolic process
D	0030060	molecular_function	L-malate dehydrogenase activity
D	0043490	biological_process	malate-aspartate shuttle
D	0043621	molecular_function	obsolete protein self-association
D	0046554	molecular_function	malate dehydrogenase (NADP+) activity
D	0070062	cellular_component	extracellular exosome

Functional Information from PDB Data

site_id	AC1
Number of Residues	11
Details	binding site for residue CIT A 401

Chain	Residue
A	ARG104
A	HOH512
A	HOH536
A	ARG110
A	ILE140
A	ASN142
A	ARG176
A	HIS200
A	GLY234
A	ALA247
A	MET251

---

site_id	AC2
Number of Residues	9
Details	binding site for residue CIT B 401

Chain	Residue
B	ARG104
B	ARG110
B	ASN142
B	ARG176
B	HIS200
B	GLY234
B	ALA247
B	MET251
B	HOH512

---

site_id	AC3
Number of Residues	9
Details	binding site for residue CIT C 401

Chain	Residue
C	ARG104
C	ARG110
C	ILE140
C	ASN142
C	ARG176
C	HIS200
C	GLY234
C	ALA247
C	MET251

---

site_id	AC4
Number of Residues	10
Details	binding site for residue CIT D 401

Chain	Residue
D	ARG104
D	ARG110
D	ILE140
D	ASN142
D	ARG176
D	HIS200
D	GLY234
D	ALA247
D	HOH504
D	HOH507

---

Functional Information from PROSITE/UniProt

site_id	PS00068
Number of Residues	13
Details	MDH Malate dehydrogenase active site signature. VTTLDivRAntfV

Chain	Residue	Details
A	VAL169-VAL181

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	ACT_SITE: Proton acceptor => ECO:0000250|UniProtKB:P00346

Chain	Residue	Details
A	HIS200
B	HIS200
C	HIS200
D	HIS200

---

site_id	SWS_FT_FI2
Number of Residues	20
Details	BINDING: BINDING => ECO:0000269|Ref.16

Chain	Residue	Details
A	MET251
B	GLY31
B	ASP57
B	ASN117
B	ILE140
B	MET251
C	GLY31
C	ASP57
C	ASN117
C	ILE140
C	MET251
D	GLY31
D	ASP57
D	ASN117
D	ILE140
D	MET251
A	GLY31
A	ASP57
A	ASN117
A	ILE140

---

site_id	SWS_FT_FI3
Number of Residues	16
Details	BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU10004, ECO:0000269|Ref.16

Chain	Residue	Details
D	ARG104
D	ARG110
D	ASN142
D	ARG176
B	ASN142
A	ARG104
A	ARG110
A	ASN142
A	ARG176
B	ARG104
B	ARG110
B	ARG176
C	ARG104
C	ARG110
C	ASN142
C	ARG176

---

site_id	SWS_FT_FI4
Number of Residues	36
Details	MOD_RES: N6-succinyllysine; alternate => ECO:0000250|UniProtKB:P08249

Chain	Residue	Details
B	LYS324
B	LYS335
C	LYS78
C	LYS91
C	LYS185
C	LYS215
C	LYS296
C	LYS307
C	LYS314
C	LYS324
C	LYS335
D	LYS78
D	LYS91
D	LYS185
D	LYS215
D	LYS296
D	LYS307
D	LYS314
D	LYS324
D	LYS335
A	LYS78
A	LYS91
A	LYS185
A	LYS215
A	LYS296
A	LYS307
A	LYS314
A	LYS324
A	LYS335
B	LYS78
B	LYS91
B	LYS185
B	LYS215
B	LYS296
B	LYS307
B	LYS314

---

site_id	SWS_FT_FI5
Number of Residues	4
Details	MOD_RES: N6-acetyllysine => ECO:0007744|PubMed:19608861

Chain	Residue	Details
A	LYS165
B	LYS165
C	LYS165
D	LYS165

---

site_id	SWS_FT_FI6
Number of Residues	8
Details	MOD_RES: N6-succinyllysine => ECO:0000250|UniProtKB:P08249

Chain	Residue	Details
C	LYS203
C	LYS269
D	LYS203
D	LYS269
A	LYS203
A	LYS269
B	LYS203
B	LYS269

---

site_id	SWS_FT_FI7
Number of Residues	12
Details	MOD_RES: N6-succinyllysine; alternate => ECO:0000250|UniProtKB:Q32LG3

Chain	Residue	Details
C	LYS328
D	LYS239
D	LYS301
D	LYS328
A	LYS239
A	LYS301
A	LYS328
B	LYS239
B	LYS301
B	LYS328
C	LYS239
C	LYS301

---

site_id	SWS_FT_FI8
Number of Residues	8
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:23186163

Chain	Residue	Details
A	SER246
A	SER326
C	SER246
C	SER326
D	SER246
D	SER326
B	SER246
B	SER326

---

site_id	SWS_FT_FI9
Number of Residues	4
Details	MOD_RES: N6-malonyllysine; alternate => ECO:0000250|UniProtKB:Q32LG3

Chain	Residue	Details
A	LYS329
D	LYS329
B	LYS329
C	LYS329

---

site_id	SWS_FT_FI10
Number of Residues	4
Details	CARBOHYD: O-linked (GlcNAc) serine => ECO:0000250|UniProtKB:P04636

Chain	Residue	Details
A	SER33
B	SER33
C	SER33
D	SER33

---